Status
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Gene information
General information
TC007081 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9978886..9986300
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007081
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04270

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007081 RA
      MPLFCVILV LANFFNCVS LEHVWHSYQ YDPNHQNRE 
      ELTITNITA DDQFKFNYV AKSWKSLCD DQICYLVGF 
      SNKNVFISL VTLNKEKNE FLQQKSQEI NFEENITDI 
      AIFQNWNSS LQDLEIVIV IALTTELFW YKLGEPGPF 
      LFWVWPINK NISHINFFE IEYDTHILI INQHFSQTT 
      ADLYQFELK DSPNFWLRQ KLQLSDFAQ SSAVVTSGP 
      DYFMAIPQK SLNQLSIYK TSDNHFISY KNLTSGGIT 
      SVVSFTCGH KSFFAINGM NAGIYRLVK RGFLKENLS 
      NSHFSGVNF WLPMDLSVY GDDFMLIAQ RSLFHGGHK 
      SFAVEVIAY NGDKFEEHD DIHCLYYGE EVLGLSCLP 
      DEGGIEGSA LINIDNNLG LVVPRKNRS SDLFLIKTE 
      IRQIPNPLD QQLQDLIKR KNEINNLIN IQEERYNEL 
      VAKSQQVFD LDSVSAKLD EMNANVLIM KEQIDSSPT 
      HYTSVIFNN VVEIKSNVT AGEVQVGEI GEELANTLL 
      EDIVLRDNI GVISGPKFI KNLETEDLL FENINGINS 
      NQLLYDSEN ITIDGDLVL ENHLIVRDL TTDQVNGAK 
      FEDQVINLE APIDEEIIF ENVKTDKAA VQKLNDHFI 
      PTEAQINES GQLEFESLF IPLNVTVDQ VNGENFDSF 
      LSQLCLRNI RNYIPGLVN IEGDVIVEG DARIEYLNN 
      LKFPYEYVF TDNIFPNIT GVKTFNKLV TSEVNCEGT 
      INGINTSQF ITRTTDQSI PQTTTFKNL KITESLNVD 
      GEIIGEHAS KFLPNPTLL KTDFVTAKC HFKNLEVEG 
      LIVLSDSFN GKNFKSMLS DVVYSSEEE PFIEGLKTF 
      PEGLTVRKN LIISSNSVT NVNLDSIVT KNTDQVLNL 
      SVLDGIVTF GNVEIGGFY DSVNITKLD EDLVKLDGE 
      QYISSTLIF EDELTVNKL EAEIVRRKD RFFDALDVE 
      NCVVEGDFE GKIQGFDLE EFNQNRMSF TEDQTVEAD 
      FELGHSFVT SISVNKLNN VDCSEFSSD SLQLKLVSK 
      IVAGKVDIT NLSTKSLEV DKIDNKSTM SLGGALDIT 
      KHNLTNLVF NDDVSFETV EIEKFGGVD FTTFVTNIV 
      YKNETHVTF SGAKTFPEG FTVHKLIET QKINNVVLP 
      NILRNYGVQ EIAGPLTVE GNVQFGENL TVGTLNDVS 
      VQAITDSFQ VNDSQIRIK GDVLFARLP HIHNLHINS 
      AQQFLDGMI YTDDNFRLQ GRTTFKNLA NFEKNIRVV 
      GKINNIDLP SFVSDMVMI TKDATIKST ARFTKPTFI 
      DHMIVNNNL KTHDLNNVN LTSWVVDAI FVNQGLMKG 
      KFTFENVTI VDNLRTDFI NDVNIDTLV PLKTQQNLT 
      QLCFKTINL FEDLPVGNS TNFHNLPKE FENTVLLNT 
      VQEIDSDVE FAGNVFIEN DLQLTGLLN EMEIERIVT 
      TNTDQELTG VYHFKSKVN IGDDLIVEG LVNGINVTE 
      WDSEGVKIS APYTQNITE NWVIEQDLV LEETTGSGT 
      INGLNFAKF SREIAEKRS YKYAMEKAL VDDYTNICK 
      DLTYMRDQL QNQIYKFSY LENLQMLNF QNEIEQVFS 
      FEDRDRVFL LISERKNCE SYLFTFGDK FRFVQSLHF 
      GLVAQIIPV RVNNLLYLV VRNQAFYST CKVQGTNLW 
      QVIDNHFVH VANIDEQEL LQESLVPGV FYGISGNEV 
      TEFKVLTEL DTVVRYRKW QVDEDNLAF VPRGFATGL 
      ALRTGRKLI RLQREDPIV EDFNYETEK IIRGNCTEE 
      FNPFFPGRK GSDLVVMKV GIRSNKRSL LAVASHEET 
      SVRGSLDFI KIYEDVFEG KMFHKVATY KPSSLQSLE 
      FKNGETFLV FLEDQSVLQ VYEYKGIEG FKHKLSSKI 
      QGTQLITIG VSERKLIGV VDKNKLSFM EAIMLGNQL 
      EENLQCTL
    CDS sequence
    • >TC007081 RA
      atgccgctg ttctgtgta attttagtg ttggcaaat 
      ttttttaat tgtgtgagt ttggaacat gtgtggcat 
      tcttatcaa tacgatccc aaccaccaa aaccgtgaa 
      gaactcaca attacaaac atcacagcc gacgaccaa 
      ttcaagttt aattatgtt gcaaaaagc tggaaaagt 
      ctgtgtgat gaccagatc tgttacctt gttggtttt 
      tcaaataaa aacgtgttc atttccctt gtaaccctc 
      aacaaggag aaaaacgaa tttttgcaa caaaagtcg 
      caagaaatt aattttgag gaaaatatc accgacatt 
      gcaattttc caaaactgg aactccagt ttacaagat 
      ttggaaatt gttattgtg attgcgtta acaaccgaa 
      ctgttttgg tataaattg ggcgaacct ggccctttt 
      cttttttgg gtttggccc attaacaaa aatataagt 
      cacatcaac tttttcgaa atcgagtat gacactcac 
      atattgata ataaaccaa catttttca caaaccacc 
      gccgatttg tatcaattt gaactaaaa gactcgccg 
      aatttttgg cttcgccag aaattacag ttgtctgat 
      tttgcacaa agttcagcc gtggtcact tcaggccct 
      gattatttc atggcaatt ccgcaaaaa tcgctcaac 
      cagttatca atttacaaa acgagcgat aatcatttt 
      attagttac aaaaattta accagtggt ggcattact 
      agcgttgtt tctttcacc tgtggccat aaatcattt 
      tttgccatc aacggcatg aatgcgggg atttaccga 
      ttggttaaa aggggtttc ctgaaggag aatctgtcc 
      aatagtcac ttcagcgga gtgaatttc tggttgcct 
      atggacttg tccgtttac ggtgacgat tttatgttg 
      attgctcaa cgttcgctg tttcacgga ggccataaa 
      tctttcgcc gtcgaagtg attgcctat aatggggat 
      aaattcgag gagcatgat gatatccat tgtttgtat 
      tacggggag gaggttttg ggtttatct tgtctgccg 
      gacgagggt ggcattgaa ggctctgct ttgataaac 
      attgataat aatttgggt ctggtggtt ccgcgtaaa 
      aatcgctcg agcgatttg tttttgatc aaaactgaa 
      attaggcaa attccgaac cctcttgac caacaatta 
      caagatcta atcaagcga aaaaatgaa ataaataat 
      ttgataaat attcaagaa gaaagatat aacgaattg 
      gtggcaaag tcacaacag gtgtttgat ttggattca 
      gttagcgca aaattggat gaaatgaat gcaaatgtt 
      ttgattatg aaagaacaa atcgattcg agtccgacg 
      cattacact tcggtcatt tttaacaat gttgttgaa 
      atcaaaagt aacgtaaca gctggagaa gtgcaagtt 
      ggggaaata ggcgaagaa ttggctaac actcttctt 
      gaagatatt gtcttgaga gataacatt ggggtcatt 
      tcgggccca aaatttatc aaaaatctg gaaactgaa 
      gatttgctt tttgaaaat atcaacgga attaactca 
      aatcaactt ctttacgat agtgaaaat atcacaatt 
      gatggcgat ttggtttta gagaatcat ttaattgtg 
      cgagatctc acaactgat caagtaaat ggagccaaa 
      tttgaggac caagttatc aatttggag gcgcctatt 
      gatgaggaa attattttc gaaaatgtg aaaacagac 
      aaagctgct gttcaaaaa ttaaacgac cactttatt 
      ccaacagag gcccaaatt aacgagtcg ggtcaactt 
      gaatttgag tcacttttc attccgttg aatgttaca 
      gttgatcaa gtaaacggc gaaaatttt gatagtttt 
      ttgagccag ttatgtctc cgcaatatt aggaattat 
      attcccgga ttagtaaat attgaaggg gacgtaatc 
      gttgaagga gacgccaga attgaatat ttaaacaat 
      ttgaaattc ccttatgaa tacgttttt actgataac 
      atttttcca aacattacc ggggttaaa acattcaac 
      aaattggta acgtctgaa gtcaattgc gagggaacg 
      atcaatggc atcaataca tcacaattt ataactcgc 
      accactgac caaagcata ccacaaact acaactttc 
      aaaaacctg aaaataaca gaaagtctg aacgttgac 
      ggtgaaata ataggcgaa catgcgagt aaattcctc 
      ccaaatccc actctccta aaaaccgat tttgtgacg 
      gccaaatgc cacttcaaa aatttggaa gttgaaggg 
      ttgattgtt ttatcggac agtttcaac gggaaaaat 
      ttcaagtca atgttgagc gatgtggtg tactctagt 
      gaagaggaa ccctttatt gaaggctta aaaaccttc 
      cctgagggt ttaaccgtt cgcaaaaac ttaataatt 
      agctcgaac agtgtcaca aacgtaaac ttggacagt 
      attgttaca aaaaacacc gaccaggtc ctaaatttg 
      agtgtcctt gatgggatt gtcactttt ggtaatgtt 
      gaaattgga ggtttttac gattctgtc aatatcacg 
      aaactggat gaggacttg gtcaagtta gacggggaa 
      caatacatt tcctcaact ctaattttc gaggatgaa 
      ttgactgtt aataaactt gaggctgaa attgtgcgc 
      cgaaaagac cggtttttt gacgcactt gatgttgaa 
      aattgcgtc gttgaggga gattttgag ggtaaaatc 
      caaggtttt gatttggag gaatttaac caaaatcgg 
      atgagtttc actgaagac caaacagtg gaggctgac 
      ttcgaattg ggtcattct ttcgtcact tcaatttca 
      gtcaataaa ttaaataac gttgattgc agcgagttt 
      agttcagat tcgttacaa ctgaagcta gtttcaaaa 
      attgtcgcc ggaaaggtg gacattaca aacttgtct 
      acaaaatcg cttgaagtt gataaaatt gataacaaa 
      tcaactatg agtcttggc ggtgccttg gacatcacg 
      aagcacaat ttgaccaac ctggtcttc aatgatgat 
      gtttccttt gaaaccgtc gaaattgag aaatttggt 
      ggtgttgat ttcactaca tttgttaca aatatagtt 
      tacaagaac gaaacacat gtgactttt tcgggcgct 
      aaaacattc cctgaaggt ttcacagtt cacaaattg 
      atagaaact caaaaaatt aacaacgtg gttttgccc 
      aacatttta agaaattat ggagttcag gaaattgca 
      ggcccactt actgtagag ggaaacgtc cagtttggt 
      gaaaatctc actgttgga accctcaac gatgtttca 
      gttcaagca ataacagat tcgtttcaa gtcaacgat 
      agtcaaatt cgcatcaaa ggagacgtt cttttcgcc 
      cgcttgccc cacatccac aacctccac atcaattcg 
      gcccagcaa tttttagac ggaatgatt tacaccgac 
      gacaatttc agactacaa gggcggacc actttcaaa 
      aacctggca aattttgag aaaaatatc cgagtggtt 
      ggaaaaatc aacaacatc gaccttccg tcatttgtg 
      tccgatatg gtaatgatc acgaaggac gctaccata 
      aagagcact gccagattc actaaacct actttcatc 
      gaccacatg atcgtaaac aataatctc aaaacgcac 
      gacttgaac aatgttaat ctcacttct tgggtggtt 
      gatgccatc ttcgtaaat caaggactc atgaaaggc 
      aaattcacg tttgaaaac gttacaatt gtcgataat 
      ctacgaacc gattttata aacgacgta aacatcgac 
      actttagtg ccgttaaag acccaacaa aacctaaca 
      caattgtgc ttcaagaca attaactta ttcgaagac 
      ttgcctgtg ggcaactcc accaatttt cacaattta 
      ccaaaagag ttcgaaaac acggtcctg cttaacacg 
      gtgcaagaa attgactcg gatgttgaa ttcgctggt 
      aatgttttc atcgaaaac gacttgcaa ttaacgggt 
      ttgctcaac gaaatggaa atcgagcgt attgtcaca 
      acaaacacg gaccaggaa ttgaccgga gtttatcat 
      tttaaatca aaagtcaat atcggggac gatttaatc 
      gttgaaggt ctagttaat gggattaac gtcacggag 
      tgggactca gaaggggtt aaaatatca gcaccgtac 
      acccaaaat atcaccgag aactgggtc atcgagcaa 
      gatttggtg ttggaagaa acgaccggt tctggaaca 
      ataaacggc ctcaacttt gccaagttc agtcgcgag 
      attgcggaa aaacgcagc tacaaatac gcaatggaa 
      aaggcgctc gttgacgat tacaccaac atttgcaaa 
      gacttgact tacatgcgt gaccagcta caaaaccaa 
      atctacaag ttcagttat ttggaaaat ctacaaatg 
      ctcaatttc caaaacgaa atcgagcaa gtttttagt 
      tttgaggac cgagacagg gtctttttg ttgataagt 
      gagcggaaa aattgcgag tcatattta ttcactttt 
      ggtgataag ttccgtttt gtgcaaagc ctccacttt 
      ggtttagtt gcacagatt atcccagtc cgtgttaac 
      aatttattg tatttagtc gtacgtaac caggctttc 
      tattccact tgtaaagtt caagggacc aatctttgg 
      caagtaatc gacaatcat ttcgtgcat gttgccaac 
      atcgatgag caagagctg ttgcaagag tcactagtc 
      cctggagtt ttttacggg attagtggc aacgaagtc 
      accgaattt aaggtttta acagagctg gacaccgtg 
      gtcaggtac aggaagtgg caagttgat gaagacaac 
      ttggcattc gttcctaga ggcttcgcc accggcttg 
      gcgttgcgg actgggaga aaattaatt agattgcag 
      cgtgaagac cccatcgtt gaggatttc aactacgag 
      actgaaaaa ataattaga ggtaactgt actgaagaa 
      ttcaatccg tttttcccc ggtcgtaaa ggcagtgat 
      cttgttgta atgaaagtc gggattaga agcaataaa 
      aggagttta ctggctgtt gctagtcac gaggagacc 
      agtgttagg ggcagttta gactttatt aagatttac 
      gaagatgtg ttcgaagga aaaatgttt cataaagtc 
      gcgacttac aaaccgtcg tccctacaa tcactcgag 
      tttaaaaac ggcgaaacg ttcctagtg ttcttggaa 
      gatcaaagc gttttacag gtgtacgaa tataaggga 
      atcgaaggt ttcaaacat aaattgtcc tccaaaatc 
      caaggaacg caactaatc acaattgga gtgtcggaa 
      cgcaaacta ataggggtc gtggataag aataaacta 
      agttttatg gaagctata atgttggga aaccaacta 
      gaggagaat ttacaatgt actttgtaa
    mRNA sequence
    • >TC007081 RA
      cttcttttc tgttttatc tctccaaat gtgtgtgtg 
      caaaatgcc gctgttctg tgtaatttt agtgttggc 
      aaatttttt taattgtgt gagtttgga acatgtgtg 
      gcattctta tcaatacga tcccaacca ccaaaaccg 
      tgaagaact cacaattac aaacatcac agccgacga 
      ccaattcaa gtttaatta tgttgcaaa aagctggaa 
      aagtctgtg tgatgacca gatctgtta ccttgttgg 
      tttttcaaa taaaaacgt gttcatttc ccttgtaac 
      cctcaacaa ggagaaaaa cgaattttt gcaacaaaa 
      gtcgcaaga aattaattt tgaggaaaa tatcaccga 
      cattgcaat tttccaaaa ctggaactc cagtttaca 
      agatttgga aattgttat tgtgattgc gttaacaac 
      cgaactgtt ttggtataa attgggcga acctggccc 
      ttttctttt ttgggtttg gcccattaa caaaaatat 
      aagtcacat caacttttt cgaaatcga gtatgacac 
      tcacatatt gataataaa ccaacattt ttcacaaac 
      caccgccga tttgtatca atttgaact aaaagactc 
      gccgaattt ttggcttcg ccagaaatt acagttgtc 
      tgattttgc acaaagttc agccgtggt cacttcagg 
      ccctgatta tttcatggc aattccgca aaaatcgct 
      caaccagtt atcaattta caaaacgag cgataatca 
      ttttattag ttacaaaaa tttaaccag tggtggcat 
      tactagcgt tgtttcttt cacctgtgg ccataaatc 
      attttttgc catcaacgg catgaatgc ggggattta 
      ccgattggt taaaagggg tttcctgaa ggagaatct 
      gtccaatag tcacttcag cggagtgaa tttctggtt 
      gcctatgga cttgtccgt ttacggtga cgattttat 
      gttgattgc tcaacgttc gctgtttca cggaggcca 
      taaatcttt cgccgtcga agtgattgc ctataatgg 
      ggataaatt cgaggagca tgatgatat ccattgttt 
      gtattacgg ggaggaggt tttgggttt atcttgtct 
      gccggacga gggtggcat tgaaggctc tgctttgat 
      aaacattga taataattt gggtctggt ggttccgcg 
      taaaaatcg ctcgagcga tttgttttt gatcaaaac 
      tgaaattag gcaaattcc gaaccctct tgaccaaca 
      attacaaga tctaatcaa gcgaaaaaa tgaaataaa 
      taatttgat aaatattca agaagaaag atataacga 
      attggtggc aaagtcaca acaggtgtt tgatttgga 
      ttcagttag cgcaaaatt ggatgaaat gaatgcaaa 
      tgttttgat tatgaaaga acaaatcga ttcgagtcc 
      gacgcatta cacttcggt catttttaa caatgttgt 
      tgaaatcaa aagtaacgt aacagctgg agaagtgca 
      agttgggga aataggcga agaattggc taacactct 
      tcttgaaga tattgtctt gagagataa cattggggt 
      catttcggg cccaaaatt tatcaaaaa tctggaaac 
      tgaagattt gctttttga aaatatcaa cggaattaa 
      ctcaaatca acttcttta cgatagtga aaatatcac 
      aattgatgg cgatttggt tttagagaa tcatttaat 
      tgtgcgaga tctcacaac tgatcaagt aaatggagc 
      caaatttga ggaccaagt tatcaattt ggaggcgcc 
      tattgatga ggaaattat tttcgaaaa tgtgaaaac 
      agacaaagc tgctgttca aaaattaaa cgaccactt 
      tattccaac agaggccca aattaacga gtcgggtca 
      acttgaatt tgagtcact tttcattcc gttgaatgt 
      tacagttga tcaagtaaa cggcgaaaa ttttgatag 
      ttttttgag ccagttatg tctccgcaa tattaggaa 
      ttatattcc cggattagt aaatattga aggggacgt 
      aatcgttga aggagacgc cagaattga atatttaaa 
      caatttgaa attccctta tgaatacgt ttttactga 
      taacatttt tccaaacat taccggggt taaaacatt 
      caacaaatt ggtaacgtc tgaagtcaa ttgcgaggg 
      aacgatcaa tggcatcaa tacatcaca atttataac 
      tcgcaccac tgaccaaag cataccaca aactacaac 
      tttcaaaaa cctgaaaat aacagaaag tctgaacgt 
      tgacggtga aataatagg cgaacatgc gagtaaatt 
      cctcccaaa tcccactct cctaaaaac cgattttgt 
      gacggccaa atgccactt caaaaattt ggaagttga 
      agggttgat tgttttatc ggacagttt caacgggaa 
      aaatttcaa gtcaatgtt gagcgatgt ggtgtactc 
      tagtgaaga ggaaccctt tattgaagg cttaaaaac 
      cttccctga gggtttaac cgttcgcaa aaacttaat 
      aattagctc gaacagtgt cacaaacgt aaacttgga 
      cagtattgt tacaaaaaa caccgacca ggtcctaaa 
      tttgagtgt ccttgatgg gattgtcac ttttggtaa 
      tgttgaaat tggaggttt ttacgattc tgtcaatat 
      cacgaaact ggatgagga cttggtcaa gttagacgg 
      ggaacaata catttcctc aactctaat tttcgagga 
      tgaattgac tgttaataa acttgaggc tgaaattgt 
      gcgccgaaa agaccggtt ttttgacgc acttgatgt 
      tgaaaattg cgtcgttga gggagattt tgagggtaa 
      aatccaagg ttttgattt ggaggaatt taaccaaaa 
      tcggatgag tttcactga agaccaaac agtggaggc 
      tgacttcga attgggtca ttctttcgt cacttcaat 
      ttcagtcaa taaattaaa taacgttga ttgcagcga 
      gtttagttc agattcgtt acaactgaa gctagtttc 
      aaaaattgt cgccggaaa ggtggacat tacaaactt 
      gtctacaaa atcgcttga agttgataa aattgataa 
      caaatcaac tatgagtct tggcggtgc cttggacat 
      cacgaagca caatttgac caacctggt cttcaatga 
      tgatgtttc ctttgaaac cgtcgaaat tgagaaatt 
      tggtggtgt tgatttcac tacatttgt tacaaatat 
      agtttacaa gaacgaaac acatgtgac tttttcggg 
      cgctaaaac attccctga aggtttcac agttcacaa 
      attgataga aactcaaaa aattaacaa cgtggtttt 
      gcccaacat tttaagaaa ttatggagt tcaggaaat 
      tgcaggccc acttactgt agagggaaa cgtccagtt 
      tggtgaaaa tctcactgt tggaaccct caacgatgt 
      ttcagttca agcaataac agattcgtt tcaagtcaa 
      cgatagtca aattcgcat caaaggaga cgttctttt 
      cgcccgctt gccccacat ccacaacct ccacatcaa 
      ttcggccca gcaattttt agacggaat gatttacac 
      cgacgacaa tttcagact acaagggcg gaccacttt 
      caaaaacct ggcaaattt tgagaaaaa tatccgagt 
      ggttggaaa aatcaacaa catcgacct tccgtcatt 
      tgtgtccga tatggtaat gatcacgaa ggacgctac 
      cataaagag cactgccag attcactaa acctacttt 
      catcgacca catgatcgt aaacaataa tctcaaaac 
      gcacgactt gaacaatgt taatctcac ttcttgggt 
      ggttgatgc catcttcgt aaatcaagg actcatgaa 
      aggcaaatt cacgtttga aaacgttac aattgtcga 
      taatctacg aaccgattt tataaacga cgtaaacat 
      cgacacttt agtgccgtt aaagaccca acaaaacct 
      aacacaatt gtgcttcaa gacaattaa cttattcga 
      agacttgcc tgtgggcaa ctccaccaa ttttcacaa 
      tttaccaaa agagttcga aaacacggt cctgcttaa 
      cacggtgca agaaattga ctcggatgt tgaattcgc 
      tggtaatgt tttcatcga aaacgactt gcaattaac 
      gggtttgct caacgaaat ggaaatcga gcgtattgt 
      cacaacaaa cacggacca ggaattgac cggagttta 
      tcattttaa atcaaaagt caatatcgg ggacgattt 
      aatcgttga aggtctagt taatgggat taacgtcac 
      ggagtggga ctcagaagg ggttaaaat atcagcacc 
      gtacaccca aaatatcac cgagaactg ggtcatcga 
      gcaagattt ggtgttgga agaaacgac cggttctgg 
      aacaataaa cggcctcaa ctttgccaa gttcagtcg 
      cgagattgc ggaaaaacg cagctacaa atacgcaat 
      ggaaaaggc gctcgttga cgattacac caacatttg 
      caaagactt gacttacat gcgtgacca gctacaaaa 
      ccaaatcta caagttcag ttatttgga aaatctaca 
      aatgctcaa tttccaaaa cgaaatcga gcaagtttt 
      tagttttga ggaccgaga cagggtctt tttgttgat 
      aagtgagcg gaaaaattg cgagtcata tttattcac 
      ttttggtga taagttccg ttttgtgca aagcctcca 
      ctttggttt agttgcaca gattatccc agtccgtgt 
      taacaattt attgtattt agtcgtacg taaccaggc 
      tttctattc cacttgtaa agttcaagg gaccaatct 
      ttggcaagt aatcgacaa tcatttcgt gcatgttgc 
      caacatcga tgagcaaga gctgttgca agagtcact 
      agtccctgg agtttttta cgggattag tggcaacga 
      agtcaccga atttaaggt tttaacaga gctggacac 
      cgtggtcag gtacaggaa gtggcaagt tgatgaaga 
      caacttggc attcgttcc tagaggctt cgccaccgg 
      cttggcgtt gcggactgg gagaaaatt aattagatt 
      gcagcgtga agaccccat cgttgagga tttcaacta 
      cgagactga aaaaataat tagaggtaa ctgtactga 
      agaattcaa tccgttttt ccccggtcg taaaggcag 
      tgatcttgt tgtaatgaa agtcgggat tagaagcaa 
      taaaaggag tttactggc tgttgctag tcacgagga 
      gaccagtgt taggggcag tttagactt tattaagat 
      ttacgaaga tgtgttcga aggaaaaat gtttcataa 
      agtcgcgac ttacaaacc gtcgtccct acaatcact 
      cgagtttaa aaacggcga aacgttcct agtgttctt 
      ggaagatca aagcgtttt acaggtgta cgaatataa 
      gggaatcga aggtttcaa acataaatt gtcctccaa 
      aatccaagg aacgcaact aatcacaat tggagtgtc 
      ggaacgcaa actaatagg ggtcgtgga taagaataa 
      actaagttt tatggaagc tataatgtt gggaaacca 
      actagagga gaatttaca atgtacttt gtaatcttt 
      attaataaa atttattgt tggca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY