Status
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Gene information
General information
TC007061 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10266892..10268785
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007061
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04265

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007061 RA
      MVVVEESLN HSLNLGNSV LISLGVVAV ILAVYHFWL 
      QSLRYTKLG NKIPGYDPL PIIGNAHMV MNKNPTQVM 
      ELALRVASE KGSVVRFWF GSKLGVALL DPRDIELIL 
      GSNVHLEKS SEYRFFEPW LGDGLLISK GDKWRSHRK 
      MIAPTFHQS ILKTFVPVF NKNAMDLVE QLRNEALDQ 
      ICDVHDYLS GATVDVLLE TVMGVKKTK EARTSYKYA 
      KAVMDMCTI LHFRHVKLW LRSDWIFSF TKLFKEQTS 
      LLRIIHNLT DRVIKQKKK AYFERVKDG DVSLYNNAV 
      KETEEENLK IKNEQTFNF GSGLRDDLD ENDENLGEK 
      KRLAFLDFM VEASQTEGN KLNDEEIRE EVNTIMFEG 
      HDTTAAASS FFICILGVY PEIQEKVYQ ELRDIFQDS 
      DRPITFNDT LQMKYLERV LLETLRMYP PVPIITRVI 
      NEEVKLASG DYTLPVGTT VGIGQFLVH RNPKYFPNP 
      DKFDPDNFL PERCQQRHY YSFIPFSAG PRSCVGRKY 
      AMLKLKILL ASIVRNFKI KSVVKEKDF QLQADIILK 
      RADGFRVIL TSRT
    CDS sequence
    • >TC007061 RA
      atggttgtg gttgaagaa tccctaaac cattcgctc 
      aatttgggc aactctgtg ctgatttct ctgggtgtg 
      gtggctgta attttggcg gtttatcac ttttggctc 
      caatctttg cgttacaca aagctgggc aataaaata 
      cccggatat gaccccttg cccatcatt ggcaacgcc 
      catatggtc atgaacaag aatcccaca caagtcatg 
      gagctagcg ttgagagta gcctcggaa aaaggaagt 
      gttgtgagg ttttggttc gggagtaag ctgggagtt 
      gctttgcta gaccccaga gatatcgaa ttgattttg 
      ggtagtaat gttcatttg gaaaaaagt tccgagtac 
      cgatttttc gagccttgg ctcggcgac ggccttctg 
      attagtaaa ggtgacaag tggcgttcg caccgaaaa 
      atgatagcg ccaacgttc caccaatcc attttaaaa 
      actttcgtt cctgttttc aataaaaac gcgatggat 
      ttggtcgaa caattgcga aacgaagcc cttgatcaa 
      atttgtgac gttcacgac tacttgagt ggtgccact 
      gtggacgtc cttttggaa acagtgatg ggcgttaaa 
      aagaccaaa gaagcaaga actagttac aagtacgcc 
      aaagccgtg atggacatg tgcacaatt ctccatttc 
      cggcacgtc aaattatgg ctgcgttca gattggatt 
      ttcagcttt acaaaattg tttaaggag cagacttca 
      ctcttgagg attatccac aatctcacg gaccgggtc 
      atcaaacaa aaaaagaaa gcttatttc gaacgtgtc 
      aaagacggg gatgttagt ttgtacaat aacgcggtc 
      aaggaaacg gaagaggag aatctgaaa attaaaaac 
      gagcaaact tttaatttc gggagcggg cttcgggac 
      gatttggac gaaaacgat gagaatctc ggtgagaaa 
      aagcgactc gcgtttttg gatttcatg gtcgaggcg 
      agccagacc gagggaaat aaactaaac gatgaggaa 
      attcgggaa gaagttaac acaattatg ttcgaaggg 
      catgacaca acggcagct gcgtcgagt tttttcatc 
      tgtatttta ggagtctac cctgaaata caggagaaa 
      gtgtatcag gaattgagg gacattttc caagacagt 
      gacagaccg atcactttt aacgatact ttacaaatg 
      aaatattta gagcgcgtt cttttggaa accttgaga 
      atgtaccca ccggtccct atcatcaca cgagtcata 
      aacgaggaa gtcaagctt gcttcgggc gattatacg 
      ctacctgtg ggcaccacg gtggggatt ggtcaattt 
      ttggttcac cgaaatccc aaatatttc cccaatccg 
      gataagttc gatccggac aatttccta cctgaacgc 
      tgccaacag agacattac tacagtttt atccctttc 
      agtgcaggc ccccgcagt tgcgtcggc cgtaaatac 
      gcaatgctc aaactgaaa attcttctc gcttcgatc 
      gtgcgaaac ttcaagatc aaatcggtc gtcaaagaa 
      aaagatttc cagctgcag gccgacatt atcctgaaa 
      agggctgac ggtttcagg gtcatttta accagcaga 
      acatga
    mRNA sequence
    • >TC007061 RA
      gtgcgggac aattcgtgt aaaatggtt gtggttgaa 
      gaatcccta aaccattcg ctcaatttg ggcaactct 
      gtgctgatt tctctgggt gtggtggct gtaattttg 
      gcggtttat cacttttgg ctccaatct ttgcgttac 
      acaaagctg ggcaataaa atacccgga tatgacccc 
      ttgcccatc attggcaac gcccatatg gtcatgaac 
      aagaatccc acacaagtc atggagcta gcgttgaga 
      gtagcctcg gaaaaagga agtgttgtg aggttttgg 
      ttcgggagt aagctggga gttgctttg ctagacccc 
      agagatatc gaattgatt ttgggtagt aatgttcat 
      ttggaaaaa agttccgag taccgattt ttcgagcct 
      tggctcggc gacggcctt ctgattagt aaaggtgac 
      aagtggcgt tcgcaccga aaaatgata gcgccaacg 
      ttccaccaa tccatttta aaaactttc gttcctgtt 
      ttcaataaa aacgcgatg gatttggtc gaacaattg 
      cgaaacgaa gcccttgat caaatttgt gacgttcac 
      gactacttg agtggtgcc actgtggac gtccttttg 
      gaaacagtg atgggcgtt aaaaagacc aaagaagca 
      agaactagt tacaagtac gccaaagcc gtgatggac 
      atgtgcaca attctccat ttccggcac gtcaaatta 
      tggctgcgt tcagattgg attttcagc tttacaaaa 
      ttgtttaag gagcagact tcactcttg aggattatc 
      cacaatctc acggaccgg gtcatcaaa caaaaaaag 
      aaagcttat ttcgaacgt gtcaaagac ggggatgtt 
      agtttgtac aataacgcg gtcaaggaa acggaagag 
      gagaatctg aaaattaaa aacgagcaa acttttaat 
      ttcgggagc gggcttcgg gacgatttg gacgaaaac 
      gatgagaat ctcggtgag aaaaagcga ctcgcgttt 
      ttggatttc atggtcgag gcgagccag accgaggga 
      aataaacta aacgatgag gaaattcgg gaagaagtt 
      aacacaatt atgttcgaa gggcatgac acaacggca 
      gctgcgtcg agttttttc atctgtatt ttaggagtc 
      taccctgaa atacaggag aaagtgtat caggaattg 
      agggacatt ttccaagac agtgacaga ccgatcact 
      tttaacgat actttacaa atgaaatat ttagagcgc 
      gttcttttg gaaaccttg agaatgtac ccaccggtc 
      cctatcatc acacgagtc ataaacgag gaagtcaag 
      cttgcttcg ggcgattat acgctacct gtgggcacc 
      acggtgggg attggtcaa tttttggtt caccgaaat 
      cccaaatat ttccccaat ccggataag ttcgatccg 
      gacaatttc ctacctgaa cgctgccaa cagagacat 
      tactacagt tttatccct ttcagtgca ggcccccgc 
      agttgcgtc ggccgtaaa tacgcaatg ctcaaactg 
      aaaattctt ctcgcttcg atcgtgcga aacttcaag 
      atcaaatcg gtcgtcaaa gaaaaagat ttccagctg 
      caggccgac attatcctg aaaagggct gacggtttc 
      agggtcatt ttaaccagc agaacatga aattattag 
      tttataact gtgaatatt ttattgtgc taagaagtt 
      aaggattta ataataaat tatataatc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY