Status
Please wait ...
Gene information
General information
TC032930
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007059,TC007058
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10326709..10344545
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032930
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04264

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032930 RA
      MECDGATDE QKGPAGWEM APLGKDGPE QPWTGVDHL 
      SGPLSPLCI TDANHEILR PKPRRLSNN SKSDFNHQG 
      ETPRVNTPA NIDEGMVSP KRKVEHFQH LYIANNVKT 
      ATFNEQTDK NNIISNKNN LNNEYKNNM VPERDTDSK 
      TTPEVGLNL SPQGTSNSR QHHHVTIQE LRLQQQQRT 
      QNCNSSSDE NRSSGHASM SDNGTSSPR GTDRLTAGV 
      MQKSLKNRA SAQHNRARH RATPARLHV PWTGSGGLE 
      DIKLALQQL TMRSHTSSS TYSSISAGS ESSEPAVRR 
      LMRHSSLET INTNITNAD EFVWVDSHN RLVELQHLP 
      WTNHCILRV LQTGRFRDH VSRVSVETI PRLSYLLQR 
      ALVRIGREI QRLSSTLGI CTKHDITVS FRIVLCPPL 
      ADSCIKASL RAAAMLAMS GDCALKQSK SLRAGLQFH 
      IGRFHRWMT DIKLARFVH EYAAVYLCA GLENLLEEI 
      LLQCLPSDS EVTLTATLL EHAIANNGD LWGLLQPYA 
      HLNAGRIAS GALALPRWA SMSSIGSGR DSRTPEPSL 
      MTTCVGSLK ELQDLISRM QPRVPLCPK AMRTLFYFM 
      RCSQLEHVE QGASAVQEL CYERAYVVL PPLVEWLRV 
      ATAHSEHRF APLIDKDDV MQAARLLLP GVDCPVRNL 
      GEEMLRIRK NTGNEDDHI EASRQIQVE LAFRLLLTG 
      RPELIQHAV SLLPVSTRL DTLNLQGHT ALMLAAIND 
      DDIALTALL DAGASVDVE TPITNPNFS AVNGETQHW 
      TALTYAAAK GHARCCRIL LERGANVEG GARLSEDKC 
      TLTPLQVAC GTGNSEVVS LLLAHGAHP FFSTQIKDS 
      LCYSASAQR GCYSAISVA AAHGQRAIL QKLLAQPLI 
      PVNKEVLSL EEILAEGTT PNCSSSSNS TPHFTKTQV 
      KSLQEAMYH SAENNHLDI TVEIRALGV PWTLHCWMH 
      SLGAAHEAR LDCVIDQLL QDFLQVCPD DYSSQFVQE 
      CLPLLFNIF RYSKKEGTT LLLADIFST CFGWEPIKP 
      IKNCVPSAT SSSRIDPKY VNNPELSDV TFRVEGRLF 
      YAHKIVLVT ASSRLRSML SSKLCEGGL PTVQINDIR 
      YDIFQIVMQ FLYQGGCQT LEPAQDDIL ELMAAANFF 
      QLDGLLRYC ESRCASMLA LDNVVSMYI HAKVYNAVQ 
      LLEYCQGFL LQNMVALLT YDDSVKRLL FAKKLPNHD 
      VLAGLLNTL QNRIKTRKT QNNFKLNQN LCK
    CDS sequence
    • >TC032930 RA
      atggaatgc gatggagca acagatgag cagaagggc 
      cccgcgggc tgggagatg gccccgctt gggaaagac 
      ggccccgag cagccctgg acgggggtg gaccacctg 
      tcggggcct ctgagcccc ctgtgcatc accgatgcc 
      aaccatgag atactaagg cccaaaccg agacgatta 
      tcgaacaat tcgaaaagc gatttcaat catcaaggg 
      gagacgcca cgagtcaac actccggca aatatcgac 
      gaagggatg gtctcaccg aagcgcaaa gtcgagcat 
      ttccaacac ctctacatc gcaaataat gtgaaaacg 
      gccactttc aacgaacaa accgacaaa aacaacatt 
      atcagtaat aaaaataac ttaaacaat gagtataag 
      aataatatg gtcccggaa cgggacacc gattcgaag 
      actacccct gaagttggg ctaaatctc tcgcctcaa 
      gggacctcg aactcgcga cagcaccac catgtgaca 
      atccaggag ctccgctta caacagcaa caacgaacg 
      caaaattgt aacagttcc tccgacgaa aaccgttct 
      tcaggccat gctagcatg tccgataat ggcacaagt 
      tcccctcga ggaacagat cgtctaacg gccggagtt 
      atgcaaaag tcgctcaaa aatcgagct tcggctcaa 
      cacaatcgg gcaagacat cgagctaca ccagccagg 
      ttgcatgtt ccctggacc ggaagcgga ggtcttgaa 
      gacatcaaa ttagcgcta caacagctc acaatgcgc 
      tcacacact tcctcaagc acttactct agcattagt 
      gctgggtcc gagagctcg gaacctgca gtcaggagg 
      ctaatgagg cactcctct ctcgagacg attaacaca 
      aatattacc aacgctgat gagtttgtc tgggtcgat 
      tcgcataat cgcctcgtt gagctgcag cacctaccc 
      tggaccaac cactgcatt ttgcgggtg ttgcaaacg 
      ggtcgcttt cgcgaccat gtctccaga gtcagtgtt 
      gagacaatc cccagactt tcctacctg cttcaacgt 
      gcgttggtt cggattgga cgcgaaatc caacgcctg 
      tcgtcaact ttgggcatt tgcaccaaa cacgacatc 
      actgtctcc ttccgaatc gtgctttgc cccccgttg 
      gcagactcc tgcatcaaa gcatcgctc cgagccgct 
      gctatgctg gcaatgtct ggggactgt gccctcaaa 
      cagagcaaa tcgctaagg gctggtttg caattccat 
      atcgggagg ttccaccgc tggatgacc gacatcaaa 
      ctcgcaaga tttgtccat gaatatgca gcagtgtac 
      ctctgcgcc ggcttggag aacctcctc gaggagatc 
      ctcctccag tgtctccca agcgactcg gaggtgacc 
      ctcacggcc accctcctc gagcacgcc attgccaac 
      aatggagac ctatggggg ctgctgcag ccctacgcg 
      cacctcaac gctgggaga atcgcgtcg ggagcttta 
      gccctccct cgatgggcc tctatgtcc agtatcggc 
      tccggtcgc gattcccga actccagaa ccctcactc 
      atgaccact tgtgtcggg tctttgaaa gagttgcaa 
      gatttgatc agtcggatg caaccccga gtgccatta 
      tgccccaaa gcaatgcga acgcttttc tactttatg 
      aggtgttcg caactggag catgtcgaa cagggcgcc 
      agtgctgtc caggagttg tgctatgaa agagcctat 
      gttgtcctc ccgccactt gtggagtgg ttgagggtt 
      gcgacagct cacagcgag cacagattc gcgcccctt 
      atcgataaa gacgacgta atgcaagct gcaagactc 
      ttgttaccc ggagtcgac tgcccggtg agaaattta 
      ggcgaggaa atgctaaga atccggaag aacaccgga 
      aacgaagac gatcatatt gaagcttcg aggcagatt 
      caggtcgaa ctggcgttc cggttgttg ttgaccgga 
      cgtccggag ctgatacaa catgccgtt tcattgctt 
      ccggtctcg acaagactt gacactttg aatttgcaa 
      ggacacaca gcgctgatg ttggctgcg atcaacgat 
      gatgatatt gccttgact gctttgttg gacgctggg 
      gcttccgtc gatgtggaa accccaatt actaacccc 
      aattttagc gcagtgaac ggggaaacg caacattgg 
      accgctttg acatacgct gccgcaaaa ggacatgcc 
      cgctgttgc aggattttg ctagaaaga ggggccaat 
      gttgagggc ggagcacga ttaagtgaa gataaatgc 
      accctaacc ccccttcag gtggcgtgc ggaaccggc 
      aattcggag gtggtttcg ctcctactg gcccacggc 
      gcgcacccc tttttctcc acccaaatc aaagactcc 
      ctctgttac tccgcctca gctcaaagg ggttgctac 
      agcgcaatt tccgtagct gcagcccat ggccaaaga 
      gcaatcttg cagaaacta ctagcacaa cccttaatt 
      cctgtaaac aaagaagtc ttgtctttg gaggagatt 
      ctagctgag ggaaccaca cccaactgt tcctcttca 
      tcaaactcg acgcctcac tttacgaaa acccaagtc 
      aagtcgttg caagaggcc atgtatcac agcgccgag 
      aacaaccac ttggacatt acggtggaa atcagggct 
      ttgggggtc ccttggacc ctccactgt tggatgcac 
      tccctgggg gccgctcat gaagcgcgt ctcgactgc 
      gtcatcgac cagctcttg caagacttc ctgcaagtg 
      tgccccgac gattacagc tcgcagttt gtgcaagag 
      tgtctcccc ctcttgttc aacattttc agatacagc 
      aaaaaggaa gggaccacg ttgttgcta gccgatatt 
      ttttcgact tgctttggc tgggagccg atcaaaccg 
      atcaaaaac tgcgtccct tcggccact tcgagctcg 
      agaattgac ccgaaatat gtaaataat cccgaactg 
      agcgatgtc acgttccga gtggagggc cgcttgttt 
      tacgcgcac aaaatagtc ctagtgact gccagctca 
      agactgagg tccatgtta agctcgaag ctgtgtgaa 
      gggggctta ccgaccgtt caaatcaac gatatcaga 
      tacgacatc ttccagatt gtgatgcag tttttgtat 
      caagggggt tgccaaaca ctagagccg gctcaagac 
      gatattttg gagctaatg gcagcggcg aattttttc 
      cagcttgat ggtttattg cgatattgc gaaagtcgg 
      tgtgcttct atgttggcg ttagataat gttgtttca 
      atgtatatt catgctaaa gtgtacaat gctgttcag 
      ttgttggag tattgtcaa gggtttttg ttgcaaaat 
      atggtcgct ttgcttact tacgatgac tcagttaag 
      agactcctc tttgcgaaa aaattaccg aatcatgat 
      gttctcgca ggattgttg aatacgctt caaaacagg 
      atcaagaca cggaaaacg caaaataat tttaagctc 
      aatcaaaat ctgtgtaaa taa
    mRNA sequence
    • >TC032930 RA
      tacttttac caaaatcgt aaaattttc gctcccggt 
      cttaataat ttgttaaat ttgtttgta ttgtgttta 
      ttgaatgaa aaagctgtt tcttcctca aacaaatcg 
      gttttacag tagcaccga atccgaata ccttggcac 
      accagcgca tgtaatacc actcattat cttggtatt 
      tccaaccgg attttttgc tttcagcac caacaaatt 
      tcgttttag cattcgcct tcttcccaa actcttcag 
      ttttaaacc gtcgcagtt tccagcaat ttttcagca 
      atcccccgt cggaaatgt ccaaaatcg agtcgattt 
      cagtgattc cgctgaaat cgcctcacg tccccattg 
      ttaaaaacc agttcttgc gaccatagg cgcacaata 
      aattccgac ctgcgaaga ggtcgtctg gcctcacgc 
      aggcacttt tcgtccgtt tctttaggc catcccggt 
      cgtggcgct gctggtcaa ggtaatgtt tacaagttc 
      tcagtgtga aagcccttg gagagcccc aaaatgagt 
      gccctttga gccccaaat cgcccagaa tggaatgcg 
      atggagcaa cagatgagc agaagggcc ccgcgggct 
      gggagatgg ccccgcttg ggaaagacg gccccgagc 
      agccctgga cgggggtgg accacctgt cggggcctc 
      tgagccccc tgtgcatca ccgatgcca accatgaga 
      tactaaggc ccaaaccga gacgattat cgaacaatt 
      cgaaaagcg atttcaatc atcaagggg agacgccac 
      gagtcaaca ctccggcaa atatcgacg aagggatgg 
      tctcaccga agcgcaaag tcgagcatt tccaacacc 
      tctacatcg caaataatg tgaaaacgg ccactttca 
      acgaacaaa ccgacaaaa acaacatta tcagtaata 
      aaaataact taaacaatg agtataaga ataatatgg 
      tcccggaac gggacaccg attcgaaga ctacccctg 
      aagttgggc taaatctct cgcctcaag ggacctcga 
      actcgcgac agcaccacc atgtgacaa tccaggagc 
      tccgcttac aacagcaac aacgaacgc aaaattgta 
      acagttcct ccgacgaaa accgttctt caggccatg 
      ctagcatgt ccgataatg gcacaagtt cccctcgag 
      gaacagatc gtctaacgg ccggagtta tgcaaaagt 
      cgctcaaaa atcgagctt cggctcaac acaatcggg 
      caagacatc gagctacac cagccaggt tgcatgttc 
      cctggaccg gaagcggag gtcttgaag acatcaaat 
      tagcgctac aacagctca caatgcgct cacacactt 
      cctcaagca cttactcta gcattagtg ctgggtccg 
      agagctcgg aacctgcag tcaggaggc taatgaggc 
      actcctctc tcgagacga ttaacacaa atattacca 
      acgctgatg agtttgtct gggtcgatt cgcataatc 
      gcctcgttg agctgcagc acctaccct ggaccaacc 
      actgcattt tgcgggtgt tgcaaacgg gtcgctttc 
      gcgaccatg tctccagag tcagtgttg agacaatcc 
      ccagacttt cctacctgc ttcaacgtg cgttggttc 
      ggattggac gcgaaatcc aacgcctgt cgtcaactt 
      tgggcattt gcaccaaac acgacatca ctgtctcct 
      tccgaatcg tgctttgcc ccccgttgg cagactcct 
      gcatcaaag catcgctcc gagccgctg ctatgctgg 
      caatgtctg gggactgtg ccctcaaac agagcaaat 
      cgctaaggg ctggtttgc aattccata tcgggaggt 
      tccaccgct ggatgaccg acatcaaac tcgcaagat 
      ttgtccatg aatatgcag cagtgtacc tctgcgccg 
      gcttggaga acctcctcg aggagatcc tcctccagt 
      gtctcccaa gcgactcgg aggtgaccc tcacggcca 
      ccctcctcg agcacgcca ttgccaaca atggagacc 
      tatgggggc tgctgcagc cctacgcgc acctcaacg 
      ctgggagaa tcgcgtcgg gagctttag ccctccctc 
      gatgggcct ctatgtcca gtatcggct ccggtcgcg 
      attcccgaa ctccagaac cctcactca tgaccactt 
      gtgtcgggt ctttgaaag agttgcaag atttgatca 
      gtcggatgc aaccccgag tgccattat gccccaaag 
      caatgcgaa cgcttttct actttatga ggtgttcgc 
      aactggagc atgtcgaac agggcgcca gtgctgtcc 
      aggagttgt gctatgaaa gagcctatg ttgtcctcc 
      cgccacttg tggagtggt tgagggttg cgacagctc 
      acagcgagc acagattcg cgcccctta tcgataaag 
      acgacgtaa tgcaagctg caagactct tgttacccg 
      gagtcgact gcccggtga gaaatttag gcgaggaaa 
      tgctaagaa tccggaaga acaccggaa acgaagacg 
      atcatattg aagcttcga ggcagattc aggtcgaac 
      tggcgttcc ggttgttgt tgaccggac gtccggagc 
      tgatacaac atgccgttt cattgcttc cggtctcga 
      caagacttg acactttga atttgcaag gacacacag 
      cgctgatgt tggctgcga tcaacgatg atgatattg 
      ccttgactg ctttgttgg acgctgggg cttccgtcg 
      atgtggaaa ccccaatta ctaacccca attttagcg 
      cagtgaacg gggaaacgc aacattgga ccgctttga 
      catacgctg ccgcaaaag gacatgccc gctgttgca 
      ggattttgc tagaaagag gggccaatg ttgagggcg 
      gagcacgat taagtgaag ataaatgca ccctaaccc 
      cccttcagg tggcgtgcg gaaccggca attcggagg 
      tggtttcgc tcctactgg cccacggcg cgcacccct 
      ttttctcca cccaaatca aagactccc tctgttact 
      ccgcctcag ctcaaaggg gttgctaca gcgcaattt 
      ccgtagctg cagcccatg gccaaagag caatcttgc 
      agaaactac tagcacaac ccttaattc ctgtaaaca 
      aagaagtct tgtctttgg aggagattc tagctgagg 
      gaaccacac ccaactgtt cctcttcat caaactcga 
      cgcctcact ttacgaaaa cccaagtca agtcgttgc 
      aagaggcca tgtatcaca gcgccgaga acaaccact 
      tggacatta cggtggaaa tcagggctt tgggggtcc 
      cttggaccc tccactgtt ggatgcact ccctggggg 
      ccgctcatg aagcgcgtc tcgactgcg tcatcgacc 
      agctcttgc aagacttcc tgcaagtgt gccccgacg 
      attacagct cgcagtttg tgcaagagt gtctccccc 
      tcttgttca acattttca gatacagca aaaaggaag 
      ggaccacgt tgttgctag ccgatattt tttcgactt 
      gctttggct gggagccga tcaaaccga tcaaaaact 
      gcgtccctt cggccactt cgagctcga gaattgacc 
      cgaaatatg taaataatc ccgaactga gcgatgtca 
      cgttccgag tggagggcc gcttgtttt acgcgcaca 
      aaatagtcc tagtgactg ccagctcaa gactgaggt 
      ccatgttaa gctcgaagc tgtgtgaag ggggcttac 
      cgaccgttc aaatcaacg atatcagat acgacatct 
      tccagattg tgatgcagt ttttgtatc aagggggtt 
      gccaaacac tagagccgg ctcaagacg atattttgg 
      agctaatgg cagcggcga attttttcc agcttgatg 
      gtttattgc gatattgcg aaagtcggt gtgcttcta 
      tgttggcgt tagataatg ttgtttcaa tgtatattc 
      atgctaaag tgtacaatg ctgttcagt tgttggagt 
      attgtcaag ggtttttgt tgcaaaata tggtcgctt 
      tgcttactt acgatgact cagttaaga gactcctct 
      ttgcgaaaa aattaccga atcatgatg ttctcgcag 
      gattgttga atacgcttc aaaacagga tcaagacac 
      ggaaaacgc aaaataatt ttaagctca atcaaaatc 
      tgtgtaaat aaattattg ttatcccga ttagttaag 
      attatttat atatttatt agtgttgcc ttgttatat 
      cataggatt tttatgttt atcgtaaaa taaactagg 
      tacttgcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY