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Gene information
General information
TC007055 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10391252..10393723
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007055
    iBeetle screen iB_04263

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007055 RA
      MVLVVGLVT LLVLTPCLG GNCPSPCTC KWKGGKQTV 
      ECTERGLIT IPESVDPET QVLDLSGNN LQILPRETF 
      VRSGLLNLQ RVFLRRCRI GQIDDLAFR GLTNLIELD 
      LSHNLLTAV PSGTFRDVP FLRDLVLAY NPIQKIDSQ 
      AFKTIPGLI KLDLSNCEI QVIASKAFE GIEMLESLK 
      LNGNRLSEL RLRTVETLN RLHGIEMHD NPWHCDCRL 
      RAVKEWLVN NNIPYPISP ICSGGPERL IDKTFTELH 
      IDDFACKPE ILPVNRYIE ATSGSNATI LCRANAIPV 
      ANINWYWNG RPLLNNSAF SSHQRIHVF EEGKQEKRS 
      VLVLTNVQE INSSEFYCV AENRAGNAE ANFTLHVSM 
      RAAGITTLG SGQIAGLSV ALIILIIFI LLVIFVLLV 
      RLRRIPFSE SKTSGQIEV VTVVNGTSK VEEPSHEHT 
      KPTELNFCN PVQKPPRLS DSTIPASCF VSPSSASGN 
      NPDLINDTK PSEDPEGLE RPASGEYSR NADSLYPSG 
      LWETDYSRQ VNFYDDKTP IMDGMSGGG SLEELAFRA 
      QGYPADYGL PIADKQQTL PTNAKTLRV WQKGGVPVL 
      PPVTALKRA LTHSRNSPD EGYQEGCGT DV
    CDS sequence
    • >TC007055 RA
      atggtgttg gtggtgggg ctggtgacc ctcctagtg 
      ctcaccccg tgcttaggg ggcaactgc ccgtccccg 
      tgcacctgc aagtggaaa gggggcaag caaacagtg 
      gagtgcacg gagcgagga ctcatcacc atccccgag 
      agcgtcgat ccggagacg caagtattg gatttgtcc 
      ggaaataat ttgcagatc ctgcccaga gagacgttc 
      gttcggtcg gggctactc aacctccag agagtgttt 
      ctaagacgt tgtcggatc gggcaaatc gacgatctc 
      gcctttaga ggattgacg aacttgatc gagttggac 
      ttgtcgcat aatctactg acggcggtt ccgtccggg 
      acgttccgg gacgtaccg ttcttacgc gatttggta 
      cttgcgtac aacccgatt caaaaaata gacagtcag 
      gcttttaaa actatcccg ggacttatt aaactcgat 
      ttgtcgaat tgtgaaata caagttatt gcttcgaag 
      gcgtttgaa gggattgaa atgttggag tcactcaag 
      ttgaacgga aaccggctc tcggagtta cgtttgagg 
      actgtggag acgctaaac agactccat gggattgaa 
      atgcacgac aatccctgg cattgtgat tgtcggttg 
      cgagcagtc aaagaatgg ctcgttaat aacaatatt 
      ccttacccc atctcgccg atttgttcc ggaggacct 
      gaaagactc atcgataag acgtttacg gagcttcac 
      atcgatgat ttcgcttgt aaacctgaa atcctcccc 
      gtaaatcgc tacattgag gcgacttcc ggctcaaac 
      gcgacgatt ttatgccga gcgaacgcc atcccagtg 
      gctaacatc aactggtat tggaacgga cgtcccctt 
      ctgaacaac tcagctttc agctcacac caaagaatc 
      cacgttttc gaagagggc aagcaggag aagcgcagt 
      gttttagtg ttgacaaac gtccaagag atcaattcg 
      agtgaattc tattgtgtg gcggagaac agggctggg 
      aatgccgaa gccaacttc accctccac gtctcgatg 
      cgagctgca gggatcaca acgcttggc agcggccaa 
      atcgccggt ctcagtgtc gctctcata attctaata 
      atctttatc ctcctggtc attttcgtg ctgcttgtc 
      cgcctccgc cgcatcccc ttttccgag agcaaaacc 
      tccggtcag attgaagtc gtaactgtg gtcaacgga 
      acgtccaaa gtggaggaa ccctctcac gagcatacc 
      aagccgacc gagttgaat ttctgcaat ccagtccaa 
      aagccgccc cgtttgagc gactccacg atcccagct 
      tcctgtttc gtgtctccc tcgtcggcc tccgggaac 
      aaccctgac ctaatcaac gacaccaaa ccgtcggag 
      gaccccgaa gggttggag aggcccgcc agcggcgag 
      tacagtcgc aacgctgat tcgctctat ccctcggga 
      ttatgggag acggactac tcgcgtcag gtgaatttc 
      tacgacgat aagacgccc attatggac gggatgagt 
      ggcggaggg tcgctggaa gagttggcg ttcagggcg 
      caaggatat ccggctgat tatgggctt cccatcgct 
      gacaaacaa caaactttg cccacgaat gcgaaaacg 
      ctgagggtg tggcagaag ggaggtgtt cccgtttta 
      cctccggtg acggcactc aaaagagcg ctgactcac 
      agcagaaat tcgccggat gaagggtat caagaaggc 
      tgcgggact gatgtttaa
    mRNA sequence
    • >TC007055 RA
      ggcccgagg cgagacaac gaggaagga gtagacagc 
      cctctcatt cagtcatcg ttcggcgtc cgtgatgtt 
      cgcgcaaag accagtctt cgtggggct aaccactta 
      atcagtcgt cggcgcgtc gtgcaacat ggtgttggt 
      ggtggggct ggtgaccct cctagtgct caccccgtg 
      cttaggggg caactgccc gtccccgtg cacctgcaa 
      gtggaaagg gggcaagca aacagtgga gtgcacgga 
      gcgaggact catcaccat ccccgagag cgtcgatcc 
      ggagacgca agtattgga tttgtccgg aaataattt 
      gcagatcct gcccagaga gacgttcgt tcggtcggg 
      gctactcaa cctccagag agtgtttct aagacgttg 
      tcggatcgg gcaaatcga cgatctcgc ctttagagg 
      attgacgaa cttgatcga gttggactt gtcgcataa 
      tctactgac ggcggttcc gtccgggac gttccggga 
      cgtaccgtt cttacgcga tttggtact tgcgtacaa 
      cccgattca aaaaataga cagtcaggc ttttaaaac 
      tatcccggg acttattaa actcgattt gtcgaattg 
      tgaaataca agttattgc ttcgaaggc gtttgaagg 
      gattgaaat gttggagtc actcaagtt gaacggaaa 
      ccggctctc ggagttacg tttgaggac tgtggagac 
      gctaaacag actccatgg gattgaaat gcacgacaa 
      tccctggca ttgtgattg tcggttgcg agcagtcaa 
      agaatggct cgttaataa caatattcc ttaccccat 
      ctcgccgat ttgttccgg aggacctga aagactcat 
      cgataagac gtttacgga gcttcacat cgatgattt 
      cgcttgtaa acctgaaat cctccccgt aaatcgcta 
      cattgaggc gacttccgg ctcaaacgc gacgatttt 
      atgccgagc gaacgccat cccagtggc taacatcaa 
      ctggtattg gaacggacg tccccttct gaacaactc 
      agctttcag ctcacacca aagaatcca cgttttcga 
      agagggcaa gcaggagaa gcgcagtgt tttagtgtt 
      gacaaacgt ccaagagat caattcgag tgaattcta 
      ttgtgtggc ggagaacag ggctgggaa tgccgaagc 
      caacttcac cctccacgt ctcgatgcg agctgcagg 
      gatcacaac gcttggcag cggccaaat cgccggtct 
      cagtgtcgc tctcataat tctaataat ctttatcct 
      cctggtcat tttcgtgct gcttgtccg cctccgccg 
      catcccctt ttccgagag caaaacctc cggtcagat 
      tgaagtcgt aactgtggt caacggaac gtccaaagt 
      ggaggaacc ctctcacga gcataccaa gccgaccga 
      gttgaattt ctgcaatcc agtccaaaa gccgccccg 
      tttgagcga ctccacgat cccagcttc ctgtttcgt 
      gtctccctc gtcggcctc cgggaacaa ccctgacct 
      aatcaacga caccaaacc gtcggagga ccccgaagg 
      gttggagag gcccgccag cggcgagta cagtcgcaa 
      cgctgattc gctctatcc ctcgggatt atgggagac 
      ggactactc gcgtcaggt gaatttcta cgacgataa 
      gacgcccat tatggacgg gatgagtgg cggagggtc 
      gctggaaga gttggcgtt cagggcgca aggatatcc 
      ggctgatta tgggcttcc catcgctga caaacaaca 
      aactttgcc cacgaatgc gaaaacgct gagggtgtg 
      gcagaaggg aggtgttcc cgttttacc tccggtgac 
      ggcactcaa aagagcgct gactcacag cagaaattc 
      gccggatga agggtatca agaaggctg cgggactga 
      tgtttaagt gtgaattta ccaaagact gcgttccta 
      agacactgg ttgtacata atttaagtt ttctgtaac 
      cacagtgct gtgagtaca ccggaaagg ggatattta 
      gataagttt gtatttcct tatgtgata attgtttcg 
      tagcgaaac ttgtaaata ttgtgtata gactgtaga 
      taatgttgt ttatggcgt tatgaaatt catagacta 
      agtatacac gctcaaaag actgaccat tatatttac 
      caactcaaa attcatttc tagatcccc atttcattt 
      atttttcta tttattgta aatgttcat tgtcagcag 
      ctatagcaa agattttta tgtaacata atagtgtat 
      tgttgtttg caacagacg tgtaaatgt taatatatt 
      acaatgtta taccctaaa ttaacggta cctaatatc 
      aattaataa taataattc agtattctt aatgttacc 
      aacatgtat ataaatgtg aaaacggtg cgtaacttt 
      tggtaataa acaatttaa aagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY