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Gene information
General information
TC007032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11288914..11294871
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007032
    iBeetle screen iB_04259

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007032 RA
      MTFRFVPFL VLILVASTY SKHNDDFES VKHGITQLF 
      KTGNKPYFA KTAKSYQLP HVLREHMEK VYNNEEICT 
      LCNLLFDLV IDEWKQGMT KDKILDIVK YICVTLGIE 
      NDRVCNGSI TINADIVFY IVENYPDIK ANRLCGSVL 
      QAFGCSTGD DFDWSIILP PGNAAKRPQ PDTSGSSFN 
      ILQLSDIHY DPNYKINGK ANCGEPVCC QEDQGEADS 
      PENACGYWS DYRFADTPW HLVEETIRQ TKTQDFDYV 
      YYTGDIISH RVWQTTVEN NTKTISKLY SFFKQSYDV 
      PVFPIFGNH EPHPLNVWP DDRLKDETL SVKWLFKLA 
      ADEWSKLIG QDVSETVLK GGYYSVSPR PGFRIIGVN 
      GNLCYTDNW WLIYDDVDP FNQLQWLAD TLEQAEAAN 
      ESVHILSHV PTGDISCLK VWSREYHRI VERFSSTIT 
      GQFNGHTHR DEFHVYYNS SAPTEAIGV AFNGASVTP 
      YDSSNPSYK LYRVDQSTY SLLDYEEWT FNLTLANSQ 
      PVSETPEWY KLYSFAQAY GVNNLQAIE VDKVLYKMA 
      QDHSLLDDY FRFKFRNGD DGINAGCSE TCRKENLCD 
      IAKAQFADN TQCDYFLQL YEQGKQT
    CDS sequence
    • >TC007032 RA
      atgacattt cgctttgtt ccatttctc gttttaata 
      ttagttgca tcaacttat tcaaaacac aacgatgat 
      tttgagtcg gttaaacat ggcattacc caacttttc 
      aaaactggg aacaaaccc tattttgct aaaactgca 
      aaatcgtac caattacca cacgtgttg agagaacac 
      atggaaaaa gtctacaac aatgaggaa atctgcact 
      ttgtgtaac ctactcttc gatttggtg atcgacgaa 
      tggaagcaa gggatgacc aaagacaag atcttagac 
      attgtcaag tacatttgt gtcacgttg ggaatcgaa 
      aatgatcga gtttgcaac ggttcgatc actattaac 
      gctgatatt gttttctac atcgttgaa aactaccct 
      gatatcaaa gccaataga ctgtgtggc tccgttttg 
      caagctttc ggttgttca accggtgat gacttcgac 
      tggtccata attctcccg ccgggaaat gcagccaaa 
      agaccgcaa ccagacaca tctggttca agtttcaat 
      attttgcaa cttagtgac attcactac gaccctaat 
      tacaaaatc aatggcaaa gccaattgt ggggaacct 
      gtttgttgt caggaggac caaggtgaa gcagattca 
      cccgaaaat gcttgtggt tattggtcg gattatcgc 
      ttcgctgat acgccgtgg catttggtc gaagaaaca 
      ataagacaa accaaaaca caagacttc gactacgtt 
      tactacaca ggtgatata ataagtcac agggtttgg 
      caaaccact gttgaaaac aacacaaaa accatctcc 
      aagttgtac tccttcttc aaacagtca tacgatgtt 
      cctgttttt ccgattttt ggcaaccac gagcctcac 
      cccttaaat gtttggccg gatgatcgc cttaaagat 
      gagacactt tcggttaaa tggttgttt aaattagca 
      gcggatgag tggtcgaaa ttgattggg caagatgtt 
      agtgagacg gtgttaaaa ggggggtat tatagtgtc 
      agtccaagg ccagggttt aggattatt ggcgttaat 
      ggcaatttg tgttatacg gataattgg tggttgata 
      tacgacgac gtggaccct tttaaccaa ttgcaatgg 
      ctggcggac acgttagaa caagccgaa gctgccaac 
      gaaagcgtc catattttg tctcacgtc cccacaggg 
      gatatttca tgtctgaaa gtttggagt cgagaatac 
      caccgcata gtcgaaaga ttctcttcg accataacg 
      ggccagttt aacggccac actcatcgc gacgaattc 
      catgtttac tacaacagt tcggctcca actgaagcc 
      atcggtgtg gcttttaat ggggcttct gtaacacct 
      tacgacagc tccaacccc agttataaa ctttatcgt 
      gttgaccaa agcacttac tcgctttta gattatgaa 
      gaatggacg tttaatctc actttggcg aattcgcag 
      ccagttagt gaaacaccg gaatggtac aagctttac 
      agcttcgct caagcttac ggcgtgaat aatttacaa 
      gccattgaa gttgacaaa gtcctctat aagatggct 
      caagatcat tccttgctc gatgactat tttaggttc 
      aaatttcga aatggagat gatggaatt aacgccgga 
      tgtagtgag acgtgccgc aaagagaat ttatgcgat 
      attgccaaa gcacagttt gcagacaac acacaatgc 
      gattatttc ctacagctg tatgagcaa gggaaacaa 
      acctaa
    mRNA sequence
    • >TC007032 RA
      tttaaagtt tcaaaactg taccgaaca gacagtact 
      taacgataa caccatgac atttcgctt tgttccatt 
      tctcgtttt aatattagt tgcatcaac ttattcaaa 
      acacaacga tgattttga gtcggttaa acatggcat 
      tacccaact tttcaaaac tgggaacaa accctattt 
      tgctaaaac tgcaaaatc gtaccaatt accacacgt 
      gttgagaga acacatgga aaaagtcta caacaatga 
      ggaaatctg cactttgtg taacctact cttcgattt 
      ggtgatcga cgaatggaa gcaagggat gaccaaaga 
      caagatctt agacattgt caagtacat ttgtgtcac 
      gttgggaat cgaaaatga tcgagtttg caacggttc 
      gatcactat taacgctga tattgtttt ctacatcgt 
      tgaaaacta ccctgatat caaagccaa tagactgtg 
      tggctccgt tttgcaagc tttcggttg ttcaaccgg 
      tgatgactt cgactggtc cataattct cccgccggg 
      aaatgcagc caaaagacc gcaaccaga cacatctgg 
      ttcaagttt caatatttt gcaacttag tgacattca 
      ctacgaccc taattacaa aatcaatgg caaagccaa 
      ttgtgggga acctgtttg ttgtcagga ggaccaagg 
      tgaagcaga ttcacccga aaatgcttg tggttattg 
      gtcggatta tcgcttcgc tgatacgcc gtggcattt 
      ggtcgaaga aacaataag acaaaccaa aacacaaga 
      cttcgacta cgtttacta cacaggtga tataataag 
      tcacagggt ttggcaaac cactgttga aaacaacac 
      aaaaaccat ctccaagtt gtactcctt cttcaaaca 
      gtcatacga tgttcctgt ttttccgat ttttggcaa 
      ccacgagcc tcacccctt aaatgtttg gccggatga 
      tcgccttaa agatgagac actttcggt taaatggtt 
      gtttaaatt agcagcgga tgagtggtc gaaattgat 
      tgggcaaga tgttagtga gacggtgtt aaaaggggg 
      gtattatag tgtcagtcc aaggccagg gtttaggat 
      tattggcgt taatggcaa tttgtgtta tacggataa 
      ttggtggtt gatatacga cgacgtgga cccttttaa 
      ccaattgca atggctggc ggacacgtt agaacaagc 
      cgaagctgc caacgaaag cgtccatat tttgtctca 
      cgtccccac aggggatat ttcatgtct gaaagtttg 
      gagtcgaga ataccaccg catagtcga aagattctc 
      ttcgaccat aacgggcca gtttaacgg ccacactca 
      tcgcgacga attccatgt ttactacaa cagttcggc 
      tccaactga agccatcgg tgtggcttt taatggggc 
      ttctgtaac accttacga cagctccaa ccccagtta 
      taaacttta tcgtgttga ccaaagcac ttactcgct 
      tttagatta tgaagaatg gacgtttaa tctcacttt 
      ggcgaattc gcagccagt tagtgaaac accggaatg 
      gtacaagct ttacagctt cgctcaagc ttacggcgt 
      gaataattt acaagccat tgaagttga caaagtcct 
      ctataagat ggctcaaga tcattcctt gctcgatga 
      ctattttag gttcaaatt tcgaaatgg agatgatgg 
      aattaacgc cggatgtag tgagacgtg ccgcaaaga 
      gaatttatg cgatattgc caaagcaca gtttgcaga 
      caacacaca atgcgatta tttcctaca gctgtatga 
      gcaagggaa acaaaccta acttttgat taataaaat 
      aatactcgg tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY