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Gene information
General information
TC007030 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11312157..11314291
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007030
    iBeetle screen iB_06225

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04258

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007030 RA
      MAPSTFLSL LFFVTIFTL TTCKKVTIE DLEPLETGL 
      REYFQTGQA TDEFLKTLH SYEFPYLFR DGMEKFYDS 
      AAVCYMCSL VVDFVINQR KNAGVSRET ILDEATFLC 
      SELKMEAAR VCKGQMELN IDIFLYTID NYPNFTSNR 
      VCGGFLQGH NCDTGDAFE WTINIPSGN SPERPKPSG 
      PDSFTILQL SDIHYDPNY TPNGNAVCG EPVCCQPDQ 
      GEPSGPENA CGYWTDYRL GDSPWYLIE ETIRQTKTQ 
      QVDYVYYTG DIIVHRTWE TSIESNTEA ITKLYSYFK 
      DSYDVPVYP IFGNHESHP LNVWPNDKI LDDKLSIKW 
      LFELAAKHW SELIGEDVS KTVLKGGYY TVSPRPGFR 
      IIGMNSNVA YTDNWWLMY DDVDPYGQL QWLSDTLKK 
      AEDNNESVH ILTHVPTGC SYSLKVWNR EYRKILERF 
      ANTITGHFN GHTHRDEFL VYYNSSNPT QAIGAAFNG 
      ASVTPYDLS NPSFKYYHV DETTFNLLD YEEWAFNLT 
      LANSYGSSK LPEWYKLYS FVEAYGVDN LLPSEVDKV 
      LYKMTDDHS LLDDYFKFK FRNGDPGIS PGCDDGCKK 
      DLLCDIATT VFGDDAQCK RFLDLYDQ
    CDS sequence
    • >TC007030 RA
      atggcacca tctaccttc cttagttta ctgttcttc 
      gttacaatt tttacctta acgacgtgt aaaaaggtc 
      accattgag gatttggag ccacttgaa accgggctt 
      cgcgaatac ttccaaaca ggtcaagct acagacgag 
      tttctgaaa actttacat tcgtacgaa tttccttat 
      ttgtttcgc gatggaatg gaaaaattc tacgacagt 
      gcggccgtt tgctatatg tgtagctta gtcgtagat 
      tttgtgatc aatcaacga aaaaatgca ggcgttagt 
      cgtgaaact atcctcgat gaagctact ttcttgtgt 
      tctgaatta aaaatggaa gctgcgagg gtctgtaaa 
      ggccaaatg gagctcaat attgatatt ttcttgtac 
      acaattgac aattaccca aactttact tcgaatcga 
      gtttgtggt gggttcctc caaggccac aattgtgat 
      actggtgac gcttttgaa tggactatt aacatccct 
      tctggtaat tcacccgaa cgtccaaaa cccagcgga 
      ccagatagt ttcactatt ttacaactc agtgatatt 
      cactacgat cctaactat actccaaat gggaatgct 
      gtttgtggt gaacctgtt tgttgccaa ccggaccaa 
      ggagagcct tcaggtccc gaaaatgct tgtgggtac 
      tggaccgat tatagatta ggagatagt ccgtggtat 
      ttaatagag gaaacaatt aggcaaaca aaaacccag 
      caagttgat tatgtttac tacactggc gatattatc 
      gtacaccga acttgggaa actagcatt gagagcaat 
      accgaagct atcactaaa ctgtattca tactttaag 
      gatagttac gatgttcca gtttatccc atttttgga 
      aatcacgaa tctcatcct cttaacgtg tggcctaat 
      gataaaata ctcgacgat aagttgagt attaaatgg 
      ttgtttgaa ctcgcagcc aaacactgg tccgaatta 
      ataggtgaa gatgttagc aaaacggtt ttaaaaggt 
      ggttattat accgtgagt ccacgtcct ggattcagg 
      attattggg atgaacagt aacgttgct tataccgac 
      aattggtgg ttgatgtac gatgatgtt gacccatat 
      gggcaattg caatggtta agtgacact cttaaaaaa 
      gccgaagat aataacgaa agtgtccat atactgact 
      catgttcca actggttgc tcatacagt ttaaaggtt 
      tggaatcgc gaatataga aaaatcctt gaaagattt 
      gctaatacc attactgga cattttaat gggcacact 
      catagagac gagtttcta gtttactac aacagttca 
      aatccgact caagccatt ggagctgca tttaacgga 
      gcttctgta actccatac gatctttct aatccaagt 
      ttcaaatat taccacgtt gacgaaacc acttttaat 
      ttactagat tacgaagag tgggccttt aatttaacc 
      cttgccaac tcttacggc tcctccaag ttgccagaa 
      tggtacaaa ttgtacagt tttgtggaa gcttacggc 
      gtcgataat ttgctccct tctgaagtc gataaagtg 
      ttgtacaaa atgaccgac gaccactcg ctacttgac 
      gactatttc aaattcaaa ttccgaaat ggtgatcct 
      gggattagt cctgggtgc gatgatggg tgtaagaaa 
      gatttgttg tgcgatatt gcaacaact gtgtttggt 
      gacgacgcg caatgcaag cgtttccta gacttgtac 
      gaccaataa
    mRNA sequence
    • >TC007030 RA
      cgattttat actttgcca ccatggcac catctacct 
      tccttagtt tactgttct tcgttacaa tttttacct 
      taacgacgt gtaaaaagg tcaccattg aggatttgg 
      agccacttg aaaccgggc ttcgcgaat acttccaaa 
      caggtcaag ctacagacg agtttctga aaactttac 
      attcgtacg aatttcctt atttgtttc gcgatggaa 
      tggaaaaat tctacgaca gtgcggccg tttgctata 
      tgtgtagct tagtcgtag attttgtga tcaatcaac 
      gaaaaaatg caggcgtta gtcgtgaaa ctatcctcg 
      atgaagcta ctttcttgt gttctgaat taaaaatgg 
      aagctgcga gggtctgta aaggccaaa tggagctca 
      atattgata ttttcttgt acacaattg acaattacc 
      caaacttta cttcgaatc gagtttgtg gtgggttcc 
      tccaaggcc acaattgtg atactggtg acgcttttg 
      aatggacta ttaacatcc cttctggta attcacccg 
      aacgtccaa aacccagcg gaccagata gtttcacta 
      ttttacaac tcagtgata ttcactacg atcctaact 
      atactccaa atgggaatg ctgtttgtg gtgaacctg 
      tttgttgcc aaccggacc aaggagagc cttcaggtc 
      ccgaaaatg cttgtgggt actggaccg attatagat 
      taggagata gtccgtggt atttaatag aggaaacaa 
      ttaggcaaa caaaaaccc agcaagttg attatgttt 
      actacactg gcgatatta tcgtacacc gaacttggg 
      aaactagca ttgagagca ataccgaag ctatcacta 
      aactgtatt catacttta aggatagtt acgatgttc 
      cagtttatc ccatttttg gaaatcacg aatctcatc 
      ctcttaacg tgtggccta atgataaaa tactcgacg 
      ataagttga gtattaaat ggttgtttg aactcgcag 
      ccaaacact ggtccgaat taataggtg aagatgtta 
      gcaaaacgg ttttaaaag gtggttatt ataccgtga 
      gtccacgtc ctggattca ggattattg ggatgaaca 
      gtaacgttg cttataccg acaattggt ggttgatgt 
      acgatgatg ttgacccat atgggcaat tgcaatggt 
      taagtgaca ctcttaaaa aagccgaag ataataacg 
      aaagtgtcc atatactga ctcatgttc caactggtt 
      gctcataca gtttaaagg tttggaatc gcgaatata 
      gaaaaatcc ttgaaagat ttgctaata ccattactg 
      gacatttta atgggcaca ctcatagag acgagtttc 
      tagtttact acaacagtt caaatccga ctcaagcca 
      ttggagctg catttaacg gagcttctg taactccat 
      acgatcttt ctaatccaa gtttcaaat attaccacg 
      ttgacgaaa ccactttta atttactag attacgaag 
      agtgggcct ttaatttaa cccttgcca actcttacg 
      gctcctcca agttgccag aatggtaca aattgtaca 
      gttttgtgg aagcttacg gcgtcgata atttgctcc 
      cttctgaag tcgataaag tgttgtaca aaatgaccg 
      acgaccact cgctacttg acgactatt tcaaattca 
      aattccgaa atggtgatc ctgggatta gtcctgggt 
      gcgatgatg ggtgtaaga aagatttgt tgtgcgata 
      ttgcaacaa ctgtgtttg gtgacgacg cgcaatgca 
      agcgtttcc tagacttgt acgaccaat aaatttaaa 
      ttgcaaaca atatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY