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Gene information
General information
TC007019 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11648376..11649946
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007019
    iBeetle screen iB_04256

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007019 RA
      MPPNSIFVQ LLAILAMQT KCEPQTPQA RIVGGSTIV 
      IEDVPFIVS IQYQSQHFC GGSIIKPNK IITAAHCTD 
      GREASDFSI RAGSTMRES GGQVAQVKK IYQNPNFNT 
      NVNDYDVSI LELASNLSF SNTISPITL AQQEIDPNS 
      RAFTFGWGT FRSDSSRLA PELQSVALR IVDKDTCQE 
      SYEQMPITE RMVCAGSQN GGKDACQGD SGGPLVVDN 
      VLVGITSYG SGCGDPDFP GVYSNVSAL QDYIKQYL
    CDS sequence
    • >TC007019 RA
      atgccacca aattcgata ttcgtccaa cttcttgca 
      attttggca atgcaaaca aaatgcgaa cctcaaacc 
      cctcaagca cgaattgtt ggaggttca actattgtc 
      attgaagat gtccctttc atagtctcc atccaatac 
      caaagccag catttttgt ggaggctcc ataataaag 
      ccaaacaaa attatcaca gctgctcat tgcactgat 
      gggagagaa gcgtccgat ttttcaatt agagcaggc 
      tccacaatg cgtgaatca ggtggtcaa gtagcacaa 
      gtgaaaaaa atctaccaa aatcccaac tttaatacc 
      aatgttaat gactacgac gtgtcaatt cttgaactg 
      gcttcaaat ctctcgttt agcaacact atctcgccc 
      ataacttta gcccagcaa gaaatcgat ccaaattct 
      cgagctttt acgtttggt tggggcact tttcgatcg 
      gatagttcc agacttgca ccagaatta caaagtgta 
      gcgcttcga attgttgat aaagatacc tgccaggaa 
      tcgtacgag caaatgccc ataacggag cgaatggtg 
      tgtgctggg tcacaaaat ggaggaaag gatgcgtgt 
      caaggggat tcaggaggc cctttggtt gtggataat 
      gtccttgtt ggaatcacg tcatatggg tcagggtgt 
      ggcgatcct gactttcct ggagtttat tctaatgtg 
      agtgcgcta caagactac attaaacaa tatctatga
    mRNA sequence
    • >TC007019 RA
      aaatttagt gatttttta tgtatttaa tcaacaaat 
      acaaatatg aaatgcaaa tctaattct gccaatttt 
      attttttaa aataacgtt ttaatgtgt gacgttagc 
      aatttatca aaaaatatt aatgcttag ttttactgc 
      tgcatacca ttttcaaca atttaaata tcagacgat 
      tatattttt ccattaccg attaacttt agttaagta 
      aaaatgcca ccaaattcg atattcgtc caacttctt 
      gcaattttg gcaatgcaa acaaaatgc gaacctcaa 
      acccctcaa gcacgaatt gttggaggt tcaactatt 
      gtcattgaa gatgtccct ttcatagtc tccatccaa 
      taccaaagc cagcatttt tgtggaggc tccataata 
      aagccaaac aaaattatc acagctgct cattgcact 
      gatgggaga gaagcgtcc gatttttca attagagca 
      ggctccaca atgcgtgaa tcaggtggt caagtagca 
      caagtgaaa aaaatctac caaaatccc aactttaat 
      accaatgtt aatgactac gacgtgtca attcttgaa 
      ctggcttca aatctctcg tttagcaac actatctcg 
      cccataact ttagcccag caagaaatc gatccaaat 
      tctcgagct tttacgttt ggttggggc acttttcga 
      tcggatagt tccagactt gcaccagaa ttacaaagt 
      gtagcgctt cgaattgtt gataaagat acctgccag 
      gaatcgtac gagcaaatg cccataacg gagcgaatg 
      gtgtgtgct gggtcacaa aatggagga aaggatgcg 
      tgtcaaggg gattcagga ggccctttg gttgtggat 
      aatgtcctt gttggaatc acgtcatat gggtcaggg 
      tgtggcgat cctgacttt cctggagtt tattctaat 
      gtgagtgcg ctacaagac tacattaaa caatatcta 
      tgaaaatta tacacttta ttcaaattt attcagtaa 
      aagtcaagc aatgaatga ttgtgactt attttaata 
      cccattcaa aaatgatcg taaaatggt ggcttttgg 
      gcttgagtt ccacgcttg gcgccaatg catatcaac 
      tagtttctc agagtttaa cttaaatct tgaaaacta 
      tggctcgca aaaaaaacg tgacaacag attcaaaat 
      tctttatgt acaaaaaga tgtagtttg aattgtctt 
      agttcaata aaaaaaata aaaaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY