Status
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Gene information
General information
TC007018 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11654805..11665486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007018
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04255

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007018 RA
        MKFWFYFYL VLNIYEVYS QKTRYVILS RNMICTSWS 
        SSDVQWTNF PSIPKKNIL YQLPPSYLK SHSLDAHLA 
        VYFYPHQSS ILSFGDEPL HLEGAEILQ NILPIYSHL 
        IPTIQQMVL WTNEAIPKL RPVLEDVSE KNKKPQQTE 
        LTYKPLLKI RWHMDKSFH EIVPKPVLT KCAKKTTFH 
        CIDTQLEPG RKRGNAIIS YSRTANPTN RNIKWMAVV 
        KPNQSKIEL LYGSEVVLT IRWFLDKIA GPKISWVLK 
        KNDAVTAQI RCFETRENG KLKLMCMVD RNDPPLDLS 
        QFSFGKNET LEPTYQLKW FINSKSAQI KWVLYKTLK 
        KTLNKQLIH NKVNQPNDE VSHDENGFG QFMEKHETY 
        QDIGQNYNK LVETEQVDT SKHQQTDLG NHEIYEEIE 
        LDDKELLGE AAQGQNVNV ETDSKHRQT GIASSEGGS 
        FGLSIGGSL KLKNPLSGL LSGGKEASK SGGVGLAIG 
        GALKYNKNK FETNHTLKE NTRSKLKSG SKRPRLHPG 
        EKFENLPMA VQYFLKHPK QLGLKYKSP YDREWWENM 
        IEKWQPYIP KK
      CDS sequence
      • >TC007018 RA
        atgaaattc tggttttat ttttacttg gtgttgaat 
        atctatgaa gtttattca caaaaaaca cgctatgta 
        attctttca cgaaacatg atttgtacc tcatggtcc 
        agtagtgac gtgcaatgg acgaacttc ccatcaata 
        ccaaaaaaa aatattctt taccaatta ccaccaagc 
        tatttgaaa agtcattct ttggatgca catctcgct 
        gtgtatttt tatccacat cagtcgtca atattgtca 
        tttggagat gaaccgtta catcttgaa ggcgcagaa 
        atcctccaa aacatttta cccatctac agtcatcta 
        atccccact atacaacaa atggttctt tggacaaac 
        gaagctatt ccgaaactc agaccagtt ctagaagac 
        gtatcagaa aagaacaaa aaaccacaa caaacagaa 
        ctcacttat aaacctctc ctgaaaatt aggtggcac 
        atggataaa tcgtttcat gaaatcgtg cccaaacca 
        gtgttaaca aaatgtgca aaaaaaaca actttccat 
        tgtattgat acccaactc gaaccagga agaaaaaga 
        ggaaacgca attatttca tactcacga actgcgaac 
        ccaacaaat cgcaacatc aagtggatg gctgtggtt 
        aagccaaac caatcaaaa atcgagttg ttgtatggg 
        tcagaagtt gttttaact atcagatgg ttcctggat 
        aaaattgca gggccaaag atttcctgg gttttgaag 
        aaaaacgac gcagtcaca gcacaaatt cgttgtttt 
        gaaactcga gaaaatggg aaacttaaa cttatgtgt 
        atggtcgat cgaaatgac cctccttta gacttgagc 
        cagttttcg tttgggaaa aatgagact ttggaacca 
        acttatcaa ttgaaatgg ttcataaat tcgaagtcg 
        gcgcaaatt aagtgggtt ttgtataaa acattaaaa 
        aaaactttg aataaacaa ctcatacac aataaagtt 
        aatcagcca aatgatgag gtttcgcat gacgaaaat 
        ggttttgga caatttatg gagaaacat gagacttat 
        caagacatt ggacaaaat tataataaa ctcgtagaa 
        actgaacaa gtggacaca tcaaaacat cagcaaaca 
        gatttgggg aaccacgag atttatgaa gaaatagaa 
        ctagatgat aaggaatta cttggggag gctgcgcaa 
        ggtcagaac gttaacgtc gagactgat tcaaaacat 
        cggcaaaca ggtatcgca tcatctgag ggaggctct 
        tttggtttg tcgattgga ggctcccta aaactgaaa 
        aatcccctt tctggactt ttatcaggt ggtaaagaa 
        gcttccaaa agtggtggc gtcggctta gcaattgga 
        ggagctttg aaatataat aaaaataaa tttgaaaca 
        aatcacacc ttgaaagag aatacaaga agtaaactg 
        aaatccgga agtaagcga ccaagatta catccaggg 
        gagaaattt gagaattta ccaatggca gtacaatat 
        tttttaaag cacccaaag caattgggc ctaaagtat 
        aagtcccca tatgatagg gaatggtgg gaaaatatg 
        atagaaaag tggcaacct tatattcct aagaaatga
      mRNA sequence
      • >TC007018 RA
        tccttgatg tatttcttt tgtcattta atcgccgtt 
        gaaaatcac aaatgaaat caaaaagaa tgcaattaa 
        aaacattaa gactttata ttgcaataa ctctataac 
        caaattcag ttgcccttc ttccactga accgctatg 
        aaattctgg ttttatttt tacttggtg ttgaatatc 
        tatgaagtt tattcacaa aaaacacgc tatgtaatt 
        ctttcacga aacatgatt tgtacctca tggtccagt 
        agtgacgtg caatggacg aacttccca tcaatacca 
        aaaaaaaat attctttac caattacca ccaagctat 
        ttgaaaagt cattctttg gatgcacat ctcgctgtg 
        tatttttat ccacatcag tcgtcaata ttgtcattt 
        ggagatgaa ccgttacat cttgaaggc gcagaaatc 
        ctccaaaac attttaccc atctacagt catctaatc 
        cccactata caacaaatg gttctttgg acaaacgaa 
        gctattccg aaactcaga ccagttcta gaagacgta 
        tcagaaaag aacaaaaaa ccacaacaa acagaactc 
        acttataaa cctctcctg aaaattagg tggcacatg 
        gataaatcg tttcatgaa atcgtgccc aaaccagtg 
        ttaacaaaa tgtgcaaaa aaaacaact ttccattgt 
        attgatacc caactcgaa ccaggaaga aaaagagga 
        aacgcaatt atttcatac tcacgaact gcgaaccca 
        acaaatcgc aacatcaag tggatggct gtggttaag 
        ccaaaccaa tcaaaaatc gagttgttg tatgggtca 
        gaagttgtt ttaactatc agatggttc ctggataaa 
        attgcaggg ccaaagatt tcctgggtt ttgaagaaa 
        aacgacgca gtcacagca caaattcgt tgttttgaa 
        actcgagaa aatgggaaa cttaaactt atgtgtatg 
        gtcgatcga aatgaccct cctttagac ttgagccag 
        ttttcgttt gggaaaaat gagactttg gaaccaact 
        tatcaattg aaatggttc ataaattcg aagtcggcg 
        caaattaag tgggttttg tataaaaca ttaaaaaaa 
        actttgaat aaacaactc atacacaat aaagttaat 
        cagccaaat gatgaggtt tcgcatgac gaaaatggt 
        tttggacaa tttatggag aaacatgag acttatcaa 
        gacattgga caaaattat aataaactc gtagaaact 
        gaacaagtg gacacatca aaacatcag caaacagat 
        ttggggaac cacgagatt tatgaagaa atagaacta 
        gatgataag gaattactt ggggaggct gcgcaaggt 
        cagaacgtt aacgtcgag actgattca aaacatcgg 
        caaacaggt atcgcatca tctgaggga ggctctttt 
        ggtttgtcg attggaggc tccctaaaa ctgaaaaat 
        cccctttct ggactttta tcaggtggt aaagaagct 
        tccaaaagt ggtggcgtc ggcttagca attggagga 
        gctttgaaa tataataaa aataaattt gaaacaaat 
        cacaccttg aaagagaat acaagaagt aaactgaaa 
        tccggaagt aagcgacca agattacat ccaggggag 
        aaatttgag aatttacca atggcagta caatatttt 
        ttaaagcac ccaaagcaa ttgggccta aagtataag 
        tccccatat gatagggaa tggtgggaa aatatgata 
        gaaaagtgg caaccttat attcctaag aaatgataa 
        gtgaaatac atatatgct gttgtaaag tatgggttt 
        gtgttattt cattttgtt ctaataaaa taattggta 
        ataa
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