Status
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Gene information
General information
TC007014 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11802259..11819549
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007014
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04254

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings slightlysize shortened - Number of animals showing the phenotype: 5

    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007014 RA
      MPPASAHAA STTSSAAAT TAALLAWPP LLLHPWAPA 
      LLPGAWCSS AIRSALPGL LDSMKMTGP MGNPRQPAG 
      FAISDILEL DRNSNVGIE APPTDAPLY HPSHDISGY 
      VPLSRHWPT PTEALHSHH QHIPPHPAQ LSPDSTSPA 
      ISERSSVDP IGPVDNLPS SASNPAVSE RDPDEDNSE 
      DSKDPHLTD QQASGHKKR KRRVLFSKA QTYELERRF 
      RQQRYLSAP EREHLASII RLTPTQVKI WFQNHRYKT 
      KRAQHEKGM HDQQNNNPL PSPRRVAVP VLVRDGKPC 
      LSGGPKHPD PGSLQVPGA PMMMSSHGP LMYGQPRVW 
      W
    CDS sequence
    • >TC007014 RA
      atgcctccg gcgtcggca cacgccgcc tccaccacc 
      tcttcggcg gcggcgacg acagcggcg ctgctcgcc 
      tggccgccg ctgctgctg catccgtgg gcgccggct 
      cttctgccg ggggcctgg tgctcctcg gcgattaga 
      tccgctctg ccaggattg ttggatagt atgaagatg 
      actggaccg atgggaaat cccagacaa ccggcaggt 
      tttgcaatt agcgatatt ttggaattg gatcgaaac 
      agtaatgtg ggtattgaa gcacccccg acggacgca 
      cccttgtat catccctca catgacatt tcggggtat 
      gtgcccctt tccaggcac tggcccacg ccgacggaa 
      gctttacac tcccatcac cagcacatt cctccccat 
      cctgcccaa ctcagcccc gacagcacc agccccgcc 
      atctccgag cgatccagc gttgacccc atcggtccc 
      gttgacaac cttccctcc agcgcctcc aacccggcc 
      gtcagcgaa cgcgacccc gacgaagac aactccgaa 
      gactccaaa gatccccac ctcaccgac cagcaagct 
      agcggtcac aagaaacgc aaacgacga gtgcttttc 
      tccaaagcg caaacctac gagctggaa agacggttt 
      cgacagcag cgctacctc tccgctcca gaaagggaa 
      catctagcc tctatcata aggctaaca ccgacccaa 
      gtgaaaatt tggttccaa aatcacagg tataagacg 
      aaaagggcg caacacgaa aaagggatg cacgaccaa 
      caaaacaac aaccccttg ccgtcgccg cgacgcgtc 
      gcggtaccg gttttggtc cgcgacggc aagccctgt 
      ctcagtggg ggcccaaag caccccgat ccgggctcc 
      ctgcaggtc cccggagcc cccatgatg atgagttcg 
      catgggccc ctcatgtac ggccaacca agggtttgg 
      tggtga
    mRNA sequence
    • >TC007014 RA
      ataagggca gcttatcaa actgattga agtcaatat 
      gtagacagt tcagttcaa gtagtgttg tagtgtact 
      tgtatgtaa agaagttgt ccacaaaaa aaattaaaa 
      atgtctagt ggcgggtac gaagaatgc gccacctgg 
      cacatcatt ccaccggat tacgtccac atggacgac 
      gaatatcgc tacacactc cacccccgg tgtgtgtgt 
      ggcgattgg ctgtggtga gagccgtct cgagggctg 
      aagagccca tcgagtgaa gggcgcctc ttcagtggc 
      tctccagaa gctctcgac aactcgcgt gccggtcca 
      cttgagtcg ataacagtg cggtggttg tttgtgaca 
      caaagtggc cgaggaaga caagccaag ggacgacgc 
      catgcctcc ggcgtcggc acacgccgc ctccaccac 
      ctcttcggc ggcggcgac gacagcggc gctgctcgc 
      ctggccgcc gctgctgct gcatccgtg ggcgccggc 
      tcttctgcc gggggcctg gtgctcctc ggcgattag 
      atccgctct gccaggatt gttggatag tatgaagat 
      gactggacc gatgggaaa tcccagaca accggcagg 
      ttttgcaat tagcgatat tttggaatt ggatcgaaa 
      cagtaatgt gggtattga agcaccccc gacggacgc 
      acccttgta tcatccctc acatgacat ttcggggta 
      tgtgcccct ttccaggca ctggcccac gccgacgga 
      agctttaca ctcccatca ccagcacat tcctcccca 
      tcctgccca actcagccc cgacagcac cagccccgc 
      catctccga gcgatccag cgttgaccc catcggtcc 
      cgttgacaa ccttccctc cagcgcctc caacccggc 
      cgtcagcga acgcgaccc cgacgaaga caactccga 
      agactccaa agatcccca cctcaccga ccagcaagc 
      tagcggtca caagaaacg caaacgacg agtgctttt 
      ctccaaagc gcaaaccta cgagctgga aagacggtt 
      tcgacagca gcgctacct ctccgctcc agaaaggga 
      acatctagc ctctatcat aaggctaac accgaccca 
      agtgaaaat ttggttcca aaatcacag gtataagac 
      gaaaagggc gcaacacga aaaagggat gcacgacca 
      acaaaacaa caacccctt gccgtcgcc gcgacgcgt 
      cgcggtacc ggttttggt ccgcgacgg caagccctg 
      tctcagtgg gggcccaaa gcaccccga tccgggctc 
      cctgcaggt ccccggagc ccccatgat gatgagttc 
      gcatgggcc cctcatgta cggccaacc aagggtttg 
      gtggtgatt ttttcaatg ttcctcaac caaaaaaaa 
      tatttcttt tcctccgaa atgtaataa aatataatc 
      agtgcgatc agataattt cgactattg tgagtgaat 
      caaggtcga gtgagttat tttgttgtt tcggtcagg 
      tcaggtcaa atctattat gtttttcga tgtatttaa 
      aaaactttg taaatgatt gtatatgta tgtaaatag 
      gagttgtag acttgtgta cagtaggtt agaatggtt 
      aaattcagt ggccgatgt tctagtcta cattttttg 
      tggacgttt gatacttac gttcgttct caattgtac 
      aaatttatt tattaatgc gtaataaaa gacatttca 
      ccac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY