Status
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Gene information
General information
TC007012 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11963070..11978282
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007012
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04253

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    there were three eggs on the slide.
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    Adult morphology
    elytra partiallyblistered - Number of animals showing the phenotype: 7


    elytra not closed - Number of animals showing the phenotype: 7

    elytra pigmentation/tanning lighter - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007012 RA
      MHEAYQVHS LLKLTSQIE AITAYDENL LVGTRQGHL 
      FMYSLVQND EKCEVQLMR YNKSFSKKA VQQLEVIPD 
      YHLLISLTD NLIQLHDIN AINFTTVHQ VLKSKGATI 
      FAVNIQKQT SLTGVTSTL VRLAVAVKR KLQLYYLKN 
      NEFFQLMED ITLAEVPKS MVWCEETIC VGYRGEYAL 
      IELDGQQTD LFPTSSSRS SEPCIVKAS EKTFALCRE 
      SQTVLVNTK GQTEKTKAL RWSDVPLTL AWDEPFALG 
      VLSECIEVQ TLEPCGLVQ TLQDLSKVR FIITCQQGL 
      LYAASVSQV WCIRAVDVA KQRKVLLES KQFQLALKL 
      TEISNENEE DKKEKIHQI QTLLAYDLF VNKQFRESM 
      KEFLKLETD PYDVIRLFP DLLPQQTVS TDYPEPPKD 
      LTEKELETG LLALIDYLT EMRHRLQSE TQANVNARG 
      NLNEKPNFS KSTQQLLQI IDTTLLKCY LQTNDALVA 
      PLLRLNHCH LGETEKILK KMGKHNELI ILYQTKGQH 
      RRALELLQE EAGVERTIA YLQHLGTDN MGLIIEFSD 
      WVLKLSPEE GLKIFTEDL AEVEALPRP RVLDFLLRS 
      HTCVVIPYL EHVVHVWED TNPLFHNAL VHQYREKAI 
      SEGAAAEHT RKKLLEFLK KSSHYTADT VLGHFPTDS 
      LLEERAIIL GRLGKHEQS LAIYVRALG DVAKAVEYA 
      QKIYESATP GCQSVYASL IKLVLSPDS CPLALPGLT 
      LSPKTAQPD LELALELLK EHAFKMDPL EVLSILPDN 
      VPVTRIHKF LLVALQKAV KERRRVQLL KGLLYAEHL 
      QCQEMRLHL QSQHVLVTE LNVCPVCKK RFGNQSALV 
      RYPNGDVVH YSCQEKNSS AKYV
    CDS sequence
    • >TC007012 RA
      atgcatgaa gcatatcaa gttcattct ctcctaaaa 
      ttgacttcg caaattgaa gcaatcaca gcttacgat 
      gaaaatctc ttggttggc acccgacag ggtcattta 
      tttatgtat agtttggtg caaaacgac gaaaaatgc 
      gaagtccaa ttaatgaga tacaataaa tcgtttagt 
      aaaaaggca gttcaacaa ctggaggta atccccgat 
      tatcattta ctgataagt ctcactgat aatttaata 
      caattacac gacattaat gcaattaat tttacgacc 
      gttcatcag gtgttgaaa tctaaaggg gccacaatt 
      tttgcggtc aatattcag aaacagacg tctttaaca 
      ggggttact agtactttg gttaggttg gctgtggct 
      gtgaaaaga aagcttcag ttgtattat cttaaaaat 
      aatgaattt tttcaactt atggaagat attacgttg 
      gctgaggtg ccaaaatcg atggtttgg tgtgaggag 
      actatctgt gtgggctac aggggggaa tacgccctg 
      attgagttg gatggccaa caaactgac cttttcccg 
      actagcagt agccgatct agtgagcct tgtattgtc 
      aaggcctcc gaaaaaact tttgcattg tgtcgcgaa 
      tcgcaaact gttctcgta aataccaaa ggtcagacg 
      gaaaaaacc aaggccttg cgatggtca gatgttcca 
      ctgaccttg gcatgggat gaacctttt gcccttggc 
      gttctctcc gaatgtatt gaagttcaa actttggaa 
      ccatgcggt ttggttcaa accttacaa gacctttcc 
      aaggtcaga tttattatc acctgtcaa caaggtcta 
      ctctatgcg gcttctgtc agtcaagtt tggtgtatt 
      cgagctgta gatgtcgca aaacagaga aaagttttg 
      ttggaatca aagcaattc cagttggct ttgaaattg 
      acggaaatt tcaaatgag aatgaagaa gacaaaaaa 
      gagaaaatt catcagatt cagactttg ttggcttat 
      gatttattt gtgaataag caatttcgt gaaagtatg 
      aaggagttt ttgaaactt gaaactgat ccatatgat 
      gttattcga ctgttccct gatttactt ccacaacaa 
      accgtttca acagattat cctgaacca ccaaaagat 
      ttgactgaa aaagaatta gaaacggga ttgttggca 
      cttattgat tatttaact gagatgaga catcggctt 
      caatctgaa acacaggcc aacgtaaat gcccgaggc 
      aatttaaac gaaaaacca aatttcagc aaatccaca 
      caacaactt ttacaaatt attgacaca acgttactc 
      aaatgttat ttacaaacc aacgacgca ttagttgcc 
      cctttactc cgtctcaat cattgtcat ttgggtgaa 
      acggaaaaa atcctcaaa aaaatgggc aaacacaac 
      gaacttatc attttatat caaactaaa ggtcaacac 
      agacgagct ttagaacta ttgcaagaa gaagcaggc 
      gttgaacgc accatcgcc tacttgcag catttgggc 
      actgataat atggggctc attattgaa ttttccgat 
      tgggtgttg aagctctca cctgaggag ggtttaaag 
      atttttacg gaagacttg gccgaagtt gaggcactg 
      cctcgacct cgcgtcctc gacttcctc ctccgttca 
      cacacgtgt gtagttatt ccgtatctt gagcacgtg 
      gtgcatgtt tgggaggac acgaatcca ctttttcac 
      aacgcttta gtgcatcag tatcgcgaa aaggcgata 
      agtgagggc gctgcagcc gaacacact cgtaaaaaa 
      ttgctcgag tttttgaaa aaaagttca cactatact 
      gccgacact gtccttgga catttcccg actgatagt 
      ttgttagaa gagagggcg ataattttg ggacgtttg 
      ggaaaacac gaacagtct ttggcgatt tatgttcga 
      gctttgggc gatgttgcc aaagctgtg gagtatgcg 
      caaaaaata tacgaatct gcaacacca ggatgtcaa 
      agtgtgtat gccagtttg atcaaactc gtcttgagc 
      cctgacagc tgcccttta gccttacct ggcttgact 
      ctatcacct aaaacggca caacccgac ctagagttg 
      gctttggaa cttttaaaa gagcacgct ttcaaaatg 
      gatcctctt gaggtgctt tctatttta ccggataat 
      gttccagtt actcgaatc cataaattc cttcttgtg 
      gctttacaa aaagcggtt aaagaacga cgtagagtt 
      caacttttg aagggtttg ttgtatgca gaacatttg 
      caatgtcag gagatgaga ttgcattta caaagtcaa 
      catgttttg gttacggaa ttgaatgtt tgtcccgtt 
      tgtaagaaa agatttggt aatcaaagt gctttggtg 
      cgatatccg aatggagat gtggttcat tattcgtgt 
      caagagaaa aattcgagt gccaaatat gtgtaa
    mRNA sequence
    • >TC007012 RA
      tatgaaaac aacaaaatg catgaagca tatcaagtt 
      cattctctc ctaaaattg acttcgcaa attgaagca 
      atcacagct tacgatgaa aatctcttg gttggcacc 
      cgacagggt catttattt atgtatagt ttggtgcaa 
      aacgacgaa aaatgcgaa gtccaatta atgagatac 
      aataaatcg tttagtaaa aaggcagtt caacaactg 
      gaggtaatc cccgattat catttactg ataagtctc 
      actgataat ttaatacaa ttacacgac attaatgca 
      attaatttt acgaccgtt catcaggtg ttgaaatct 
      aaaggggcc acaattttt gcggtcaat attcagaaa 
      cagacgtct ttaacaggg gttactagt actttggtt 
      aggttggct gtggctgtg aaaagaaag cttcagttg 
      tattatctt aaaaataat gaatttttt caacttatg 
      gaagatatt acgttggct gaggtgcca aaatcgatg 
      gtttggtgt gaggagact atctgtgtg ggctacagg 
      ggggaatac gccctgatt gagttggat ggccaacaa 
      actgacctt ttcccgact agcagtagc cgatctagt 
      gagccttgt attgtcaag gcctccgaa aaaactttt 
      gcattgtgt cgcgaatcg caaactgtt ctcgtaaat 
      accaaaggt cagacggaa aaaaccaag gccttgcga 
      tggtcagat gttccactg accttggca tgggatgaa 
      ccttttgcc cttggcgtt ctctccgaa tgtattgaa 
      gttcaaact ttggaacca tgcggtttg gttcaaacc 
      ttacaagac ctttccaag gtcagattt attatcacc 
      tgtcaacaa ggtctactc tatgcggct tctgtcagt 
      caagtttgg tgtattcga gctgtagat gtcgcaaaa 
      cagagaaaa gttttgttg gaatcaaag caattccag 
      ttggctttg aaattgacg gaaatttca aatgagaat 
      gaagaagac aaaaaagag aaaattcat cagattcag 
      actttgttg gcttatgat ttatttgtg aataagcaa 
      tttcgtgaa agtatgaag gagtttttg aaacttgaa 
      actgatcca tatgatgtt attcgactg ttccctgat 
      ttacttcca caacaaacc gtttcaaca gattatcct 
      gaaccacca aaagatttg actgaaaaa gaattagaa 
      acgggattg ttggcactt attgattat ttaactgag 
      atgagacat cggcttcaa tctgaaaca caggccaac 
      gtaaatgcc cgaggcaat ttaaacgaa aaaccaaat 
      ttcagcaaa tccacacaa caactttta caaattatt 
      gacacaacg ttactcaaa tgttattta caaaccaac 
      gacgcatta gttgcccct ttactccgt ctcaatcat 
      tgtcatttg ggtgaaacg gaaaaaatc ctcaaaaaa 
      atgggcaaa cacaacgaa cttatcatt ttatatcaa 
      actaaaggt caacacaga cgagcttta gaactattg 
      caagaagaa gcaggcgtt gaacgcacc atcgcctac 
      ttgcagcat ttgggcact gataatatg gggctcatt 
      attgaattt tccgattgg gtgttgaag ctctcacct 
      gaggagggt ttaaagatt tttacggaa gacttggcc 
      gaagttgag gcactgcct cgacctcgc gtcctcgac 
      ttcctcctc cgttcacac acgtgtgta gttattccg 
      tatcttgag cacgtggtg catgtttgg gaggacacg 
      aatccactt tttcacaac gctttagtg catcagtat 
      cgcgaaaag gcgataagt gagggcgct gcagccgaa 
      cacactcgt aaaaaattg ctcgagttt ttgaaaaaa 
      agttcacac tatactgcc gacactgtc cttggacat 
      ttcccgact gatagtttg ttagaagag agggcgata 
      attttggga cgtttggga aaacacgaa cagtctttg 
      gcgatttat gttcgagct ttgggcgat gttgccaaa 
      gctgtggag tatgcgcaa aaaatatac gaatctgca 
      acaccagga tgtcaaagt gtgtatgcc agtttgatc 
      aaactcgtc ttgagccct gacagctgc cctttagcc 
      ttacctggc ttgactcta tcacctaaa acggcacaa 
      cccgaccta gagttggct ttggaactt ttaaaagag 
      cacgctttc aaaatggat cctcttgag gtgctttct 
      attttaccg gataatgtt ccagttact cgaatccat 
      aaattcctt cttgtggct ttacaaaaa gcggttaaa 
      gaacgacgt agagttcaa cttttgaag ggtttgttg 
      tatgcagaa catttgcaa tgtcaggag atgagattg 
      catttacaa agtcaacat gttttggtt acggaattg 
      aatgtttgt cccgtttgt aagaaaaga tttggtaat 
      caaagtgct ttggtgcga tatccgaat ggagatgtg 
      gttcattat tcgtgtcaa gagaaaaat tcgagtgcc 
      aaatatgtg taatttatt gtattgtat ataaaattg 
      gaaaaaata taatatata atcaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY