Status
Please wait ...
Gene information
General information
TC006986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451036.1: 21715..24566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006986
    iBeetle screen iB_04248

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006986 RA
      MVKTSKNIW IPMQPTSEQ VSLLSSAAT LFSLHDLYD 
      ETDIKAINR SNVLFAYDA FKSSSSEDY DSETTLYKK 
      SGSQTLRDR KWFKNACIC VISDCYRML SVIASAHKT 
      ESWQRKEEP LMYQTLEER FGFHTEENY GDFVLNANY 
      TNLHKLQKD IKHVSKLLA ELCDEIYIF HAAACLATY 
      AEAYDKMKE EEMQFMAEY LKKEQYFQQ LQRLLKTDK 
      EDTLKQFEE MDKAVDEMT ADLKDLMEY SSTCLKYTQ 
      KWQTSRQEQ NSLTLERKE NAYVSVIVG MDNKIQLEQ 
      VCHEDIERY YTLAKDDGL DEIQRWMDK YDQEYEEQE 
      MKILKIKHN TQTVVEKKE KLQSLYEVR EIEMADWLE 
      YKRMKKEQE EEEALREWA ATKIQAWWR LVMVRKKLG 
      PFRKKKKGQ KDKRKKSKR K
    CDS sequence
    • >TC006986 RA
      atggttaaa acatctaaa aatatttgg atcccaatg 
      caaccgact tcagaacaa gtcagttta ctgtcaagt 
      gcagcaaca ctgtttagt ctacatgat ctttacgat 
      gaaactgat attaaagca atcaataga tcaaatgtt 
      cttttcgca tacgatgct tttaaatcg tcatcttca 
      gaagattac gattctgaa actactcta tataaaaaa 
      agtgggtct caaacattg agagaccgt aaatggttt 
      aaaaatgcc tgcatttgc gttataagt gattgctac 
      agaatgtta tcagtgatt gcatctgca cataaaact 
      gaatcctgg cagcgaaaa gaagaacct ttgatgtat 
      caaacttta gaagaacga tttggtttt catactgaa 
      gaaaattat ggtgacttt gtattaaat gcaaattat 
      acaaacttg cataaatta caaaaagat atcaaacac 
      gtatcaaaa ctccttgct gaattgtgt gacgaaatt 
      tatattttt catgcagct gcatgtctg gctacatat 
      gctgaagcc tatgacaaa atgaaggaa gaagaaatg 
      caattcatg gctgaatat ttaaaaaaa gagcaatac 
      tttcaacaa ctgcaaagg ctattgaaa actgacaaa 
      gaagataca ctaaaacag tttgaagaa atggataaa 
      gctgtagat gaaatgact gcagacttg aaggatcta 
      atggaatat tcgagtact tgtttgaaa tacacacaa 
      aagtggcaa acatcacgc caagaacaa aactcttta 
      acacttgaa cgcaaagaa aatgcatat gttagtgta 
      atagtcggt atggacaat aaaattcag ttggagcaa 
      gtatgccac gaagatatc gaacgttat tatacattg 
      gcaaaagat gatggtttg gatgaaatc caacgttgg 
      atggacaaa tatgatcaa gaatacgaa gagcaagaa 
      atgaaaatt ttaaaaatt aaacacaac actcagacc 
      gttgtagaa aaaaaggag aaattgcaa agtttgtat 
      gaagtgcgt gaaatagaa atggcagat tggttggag 
      tataagaga atgaaaaaa gaacaagag gaggaagaa 
      gctttgaga gagtgggca gctactaaa atacaagcg 
      tggtggaga ttggtaatg gttcggaaa aaactgggc 
      ccttttagg aaaaagaaa aagggccaa aaagacaaa 
      aggaagaaa agtaagaga aagtga
    mRNA sequence
    • >TC006986 RA
      taaagtgtc aagttattg tgtcaatca gttttttcc 
      gaaccaacg aaaaaatgg ttaaaacat ctaaaaata 
      tttggatcc caatgcaac cgacttcag aacaagtca 
      gtttactgt caagtgcag caacactgt ttagtctac 
      atgatcttt acgatgaaa ctgatatta aagcaatca 
      atagatcaa atgttcttt tcgcatacg atgctttta 
      aatcgtcat cttcagaag attacgatt ctgaaacta 
      ctctatata aaaaaagtg ggtctcaaa cattgagag 
      accgtaaat ggtttaaaa atgcctgca tttgcgtta 
      taagtgatt gctacagaa tgttatcag tgattgcat 
      ctgcacata aaactgaat cctggcagc gaaaagaag 
      aacctttga tgtatcaaa ctttagaag aacgatttg 
      gttttcata ctgaagaaa attatggtg actttgtat 
      taaatgcaa attatacaa acttgcata aattacaaa 
      aagatatca aacacgtat caaaactcc ttgctgaat 
      tgtgtgacg aaatttata tttttcatg cagctgcat 
      gtctggcta catatgctg aagcctatg acaaaatga 
      aggaagaag aaatgcaat tcatggctg aatatttaa 
      aaaaagagc aatactttc aacaactgc aaaggctat 
      tgaaaactg acaaagaag atacactaa aacagtttg 
      aagaaatgg ataaagctg tagatgaaa tgactgcag 
      acttgaagg atctaatgg aatattcga gtacttgtt 
      tgaaataca cacaaaagt ggcaaacat cacgccaag 
      aacaaaact ctttaacac ttgaacgca aagaaaatg 
      catatgtta gtgtaatag tcggtatgg acaataaaa 
      ttcagttgg agcaagtat gccacgaag atatcgaac 
      gttattata cattggcaa aagatgatg gtttggatg 
      aaatccaac gttggatgg acaaatatg atcaagaat 
      acgaagagc aagaaatga aaattttaa aaattaaac 
      acaacactc agaccgttg tagaaaaaa aggagaaat 
      tgcaaagtt tgtatgaag tgcgtgaaa tagaaatgg 
      cagattggt tggagtata agagaatga aaaaagaac 
      aagaggagg aagaagctt tgagagagt gggcagcta 
      ctaaaatac aagcgtggt ggagattgg taatggttc 
      ggaaaaaac tgggccctt ttaggaaaa agaaaaagg 
      gccaaaaag acaaaagga agaaaagta agagaaagt 
      gaaaactcg acactttat tattttagg ttttatgta 
      tttatttta tttgtaatt acgtttgtg aaataacta 
      tatttcctt gaaatgaac gttttccta caaaatgaa 
      tggggagaa aagaagaaa taaatacaa ttctttatt 
      aaatagcat aagggctaa aatttataa tttacatat 
      ctacaagat gatacagag tattgaaaa gtaataata 
      actatacaa atttatcta tgtatttca aactgttct 
      aatataaat gtccagaaa taacgcagc ttcacacca 
      aattctata cttttcaat tggacgtta tgtttcaga 
      gtatttttt cattgtaaa taatgtttt tttttggca 
      ctgtgagat gaaatggtg caaatcgtt aagtacata 
      atatctttg agcggttta tctgtgatt tgcacaatt 
      tctcatgtt agttataca aataattta aataagtaa 
      aacacattt aacattggc gtcaaaaat ataaaattt 
      tatatttac agaaagaaa tgaagctta aataaaaaa 
      tctttggca gtttgttgt gaattacaa tactgtaag 
      aaacgaata aaagtacag caataacaa tattgatta 
      ttgaattta ttaatgtcg atcgatatg tgtcttaaa 
      cattataaa ttcttcaaa cacaataat ctcaatata 
      aaagtttat gatttttta tgtatttac ataattaca 
      agagatcgt cgttggtct tatttactt cttccattc 
      tattgttgt ttttgtttc ttttcatgg ctgcgtaaa 
      tggaagccg ccttgtaat ttatagggc cgtatttgt 
      aatcaacgt ctgcatatt ctaggtcac tgaattccg 
      gccggtaag gctgaaatg aaaaattcc tcttattgc 
      caacattcg gaaataaaa ctcggatat aagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY