Status
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Gene information
General information
TC006983 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451036.1: 26777..156062
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006983
    iBeetle screen iB_04247

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    iBeetle screen iB_07755

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006983 RA
      MDEMKLFGT WVLLLGFLL QYTATKETE VRALVGTNV 
      LLPCRVDVN QCGTLHSVK WYKDTSRIY VFSQAGQIR 
      RAEGDATER MNMTYNEAK NETFLQLSP VKVDDEATY 
      KCEITYLEV VENCDVIQI VKLTTLVKP SNVVLTYNG 
      KEVNESTSL EEKIEGDDV VLTCKAPGG KPIPTVSWY 
      NGTQLMNRA SYSARSDGN ATGTGISKL QLTLSKYDL 
      NARFECKVE SQALEEAIV KGVTLPVKV KPTKIKLSG 
      VNGHVNQGT IVSLICQVF GARPMAEVE WYNSTSIRM 
      VTNSSRGIE VRTVYEQDA GGTYTTKST LSFPATRFE 
      NGKRFECYG KNEVTTLKS EPDLYEPKI LDVRYPPVI 
      EVGAIGSTV VNENVNVTL TCRYFANPQ KLEGATWIK 
      DGKNLTKIP NKHYGGDVE MPSLTIIGV TREDAGDYS 
      CLLQNAVNS SISEDIIYL DVHYPPDVE VVMAPSTPA 
      NATEQTNVT LVCNVTSGN PPALRKVRW FLDGELMAE 
      LPECNYSQP VINDTIGGP FCGIDPSIL SLIKVEQTF 
      AGNYTCQGE NEAGSGPVS EPRELIVYY PPSAAKLTH 
      YPPTVMKSG QVTLTCSVE DTGFPTNVT YLWFRNGHM 
      IHNITVSNW TIPHASLET RSNFTCIAL NKGGQSKPA 
      YDFVNVEAP PTVITKASA TYKGVLYTS QNMSLSCQI 
      ECFPRCSIK WYKDGHEIN FENNKLYYV INTNHSADY 
      KTNDFESIE STLVWNMTN WPGKQLDKS APDSYYTCA 
      SYDPPDRHR VGANFSTAI GVEYPPENL TVSHSVINV 
      IEDESPIDV KCSGDGRPN LNYVWKRNQ TSEAIVKSD 
      TLKLPKMSR TKAGYYTCE AFNRHGNKT IQVKFNVWY 
      KPECKIERG EIEYNPSLI CKADGNPNE MTFTWMMIE 
      QNKTSELNP SGGNTNFSY FFLDSSVTT DIEYQCVAN 
      NSIKASDPC SIKIPGSKL PWYLSKENL IVLITSIIT 
      LIICFIIVC IVIIVLCRK KRQNTKSPN PSSTGDKTR 
      LGAAPPVGG SADPTNDHD KGFYENLPF HGMQNPPNK 
      PISIIAPFS QEMRNFTPK TPPSINPCP KITVQRHQS 
      FHGFQNTHL RFDFNRSNP FLNNCANFQ NNPQVETPF 
      SFQNNFQPI LNFNPFLLP NPFSVPPRT NRVAGRRET 
      DVRRTASGI NIAENNQVE TTKIEKSIP NDKRFGSLE 
      LKKHKCYSP TFYSMRCKK HAKKRPVVY ALPKKVFGE 
      LKPSEDKQQ QFQNLTDSI EISEQNSSP KTFPIPAPR 
      CRKHLKKDV STNNSPNPD VSNSSNEGS EHLESEISV 
      IEAQIHTSC NESETKNSS SGIIKVEAM KALKNSPML 
      KVSPNFIKP KTESPKGAL SLQIQAKRK NSTDNLNQT 
      TDDSSKENL PLIPQMPLL NPQCKWSPG LTSVKQQNA 
      FYTLTTPHY KHVNVGLPP QYSATIPKH TKLIPRALF 
      NEPLPKSKS FSSKPKHKK HFQIPLQKC HSFKFQTAE 
      SYFQPIRNI HEEDLMKNG YVSDYPPPS ESSSRTTRH 
      HKRGGEKAK QKTKGPLVV MRPNMQFPE SVQSSVQLQ 
      YPQPVLGRS VKKPNGVVY ADLDMPKTN NKKGSQHDN 
      SSCMQNKTQ KSKPKTEYA TLSFNDVGQ EIDV
    • >TC006983 RB
      MDEDEAFVK PSNVVLTYN GKEVNESTS LEEKIEGDD 
      VVLTCKAPG GKPIPTVSW YNGTQLMNR ASYSARSDG 
      NATGTGISK LQLTLSKYD LNARFECKV ESQALEEAI 
      VKGVTLPVK VKPTKIKLS GVNGHVNQG TIVSLICQV 
      FGARPMAEV EWYNSTSIR MVTNSSRGI EVRTVYEQD 
      AGGTYTTKS TLSFPATRF ENGKRFECY GKNEVTTLK 
      SEPDLYEPK ILDVRYPPV IEVGAIGST VVNENVNVT 
      LTCRYFANP QKLEGATWI KDGKNLTKI PNKHYGGDV 
      EMPSLTIIG VTREDAGDY SCLLQNAVN SSISEDIIY 
      LDVHYPPDV EVVMAPSTP ANATEQTNV TLVCNVTSG 
      NPPALRKVR WFLDGELMA ELPECNYSQ PVINDTIGG 
      PFCGIDPSI LSLIKVEQT FAGNYTCQG ENEAGSGPV 
      SEPRELIVY YPPSAAKLT HYPPTVMKS GQVTLTCSV 
      EDTGFPTNV TYLWFRNGH MIHNITVSN WTIPHASLE 
      TRSNFTCIA LNKGGQSKP AYDFVNVEA PPTVITKAS 
      ATYKGVLYT SQNMSLSCQ IECFPRCSI KWYKDGHEI 
      NFENNKLYY VINTNHSAD YKTNDFESI ESTLVWNMT 
      NWPGKQLDK SAPDSYYTC ASYDPPDRH RVGANFSTA 
      IGVEYPPEN LTVSHSVIN VIEDESPID VKCSGDGRP 
      NLNYVWKRN QTSEAIVKS DTLKLPKMS RTKAGYYTC 
      EAFNRHGNK TIQVKFNVW YKPECKIER GEIEYNPSL 
      ICKADGNPN EMTFTWMMI EQNKTSELN PSGGNTNFS 
      YFFLDSSVT TDIEYQCVA NNSIKASDP CSIKIPGSK 
      LPWYLSKEN LIVLITSII TLIICFIIV CIVIIVLCR 
      KKRQNTKSP NPSSTGDKT RLGAAPPVG GSADPTNDH 
      DKGFYENLP FHGMQNPPN KPISIIAPF SQEMRNFTP 
      KTPPSINPC PKITVQRHQ SFHGFQNTH LRFDFNRSN 
      PFLNNCANF QNNPQVETP FSFQNNFQP ILNFNPFLL 
      PNPFSVPPR TNRVAGRRE TDVRRTASG INIAENNQV 
      ETTKIEKSI PNDKRFGSL ELKKHKCYS PTFYSMRCK 
      KHAKKRPVV YALPKKVFG ELKPSEDKQ QQFQNLTDS 
      IEISEQNSS PKTFPIPAP RCRKHLKKD VSTNNSPNP 
      DVSNSSNEG SEHLESEIS VIEAQIHTS CNESETKNS 
      SSGIIKVEA MKALKNSPM LKVSPNFIK PKTESPKGA 
      LSLQIQAKR KNSTDNLNQ TTDDSSKEN LPLIPQMPL 
      LNPQCKWSP GLTSVKQQN AFYTLTTPH YKHVNVGLP 
      PQYSATIPK HTKLIPRAL FNEPLPKSK SFSSKPKHK 
      KHFQIPLQK CHSFKFQTA ESYFQPIRN IHEEDLMKN 
      GYVSDYPPP SESSSRTTR HHKRGGEKA KQKTKGPLV 
      VMRPNMQFP ESVQSSVQL QYPQPVLGR SVKKPNGVV 
      YADLDMPKT NNKKGSQHD NSSCMQNKT QKSKPKTEY 
      ATLSFNDVG QEIDV
    CDS sequence
    • >TC006983 RA
      atggatgaa atgaaactt ttcggcact tgggtgctt 
      ttgctcggc tttctactg caatataca gcaacgaag 
      gagacggaa gttcgagca cttgttggg acgaatgtg 
      ctgctgcct tgcagagtg gacgtgaac caatgtggc 
      accttgcac agcgtcaaa tggtacaag gatacgtcc 
      aggatatat gtgttcagt caagctggc cagatccga 
      cgggcggaa ggagacgct acagaaaga atgaacatg 
      acgtataat gaagccaaa aatgaaaca tttttacaa 
      ttatctccg gtgaaagta gacgacgaa gcgacttac 
      aaatgcgaa ataacatat ctggaagtg gtggagaac 
      tgtgacgtt attcaaatc gttaaactc acgacacta 
      gtcaaaccg tctaatgtt gttttgacc tacaatgga 
      aaggaagtc aacgaatca acaagtttg gaagaaaag 
      attgaaggt gatgacgtg gtgttgacg tgcaaggca 
      cctggagga aaacccatt cccacagtg tcttggtac 
      aatgggaca caactcatg aacagagcc tcttattca 
      gcgcgaagc gatggaaac gcaaccgga actggaatt 
      tccaagctg caactgacg ctttcaaaa tatgattta 
      aacgcacgc tttgaatgc aaggttgaa agtcaggct 
      ttagaagaa gcgatcgtg aaaggggtg acgcttccc 
      gttaaagtg aagccgacg aaaataaag ctgtccggt 
      gtgaatgga catgtgaat cagggaaca atcgtttca 
      cttatatgc caagttttt ggagctagg ccgatggct 
      gaagtcgaa tggtacaac agcacttcc atcagaatg 
      gtcacaaac agcagcaga ggaattgaa gttcgtaca 
      gtctacgag caagatgca ggtggtact tacacaacg 
      aaaagcaca cttagtttt cctgcaacg agattcgaa 
      aatggcaaa cgttttgaa tgttacggc aaaaatgaa 
      gtaacaaca cttaaaagc gagccagat ttatacgaa 
      ccaaaaatt ctggatgta cgatatcca ccagtgatt 
      gaagttggt gcgattgga agtacagtt gttaacgaa 
      aatgtaaat gttacttta acttgtcga tattttgca 
      aatccgcaa aaattggaa ggtgcaact tggatcaaa 
      gatggtaaa aacttgacg aaaattcca aacaagcat 
      tacggaggt gatgtggaa atgccatct cttactata 
      ataggcgtc acaagggaa gatgctggt gattatagt 
      tgtctatta cagaacgct gtgaattcg tcgatttcc 
      gaagacatt atttatttg gatgttcat tatcctcct 
      gatgtagaa gtcgtaatg gctccctcg acacctgcc 
      aatgccact gaacaaaca aatgttacg cttgtatgt 
      aacgtaaca tcgggaaat cccccagcg ctacgtaaa 
      gtgcgttgg tttctggac ggtgagctt atggctgaa 
      cttcccgaa tgcaactac agccaaccg gtgatcaac 
      gacacaatc ggaggcccc ttttgtggc attgatccc 
      agtatttta agtttgatc aaagttgag cagactttt 
      gctggaaac tacacctgt caaggggaa aatgaagct 
      ggttctgga cctgtgtct gaaccccga gagctaata 
      gtttactat cctccttct gccgccaaa ctcacacac 
      taccctcca acggttatg aaaagtggc caagttact 
      ttaacgtgc agtgtggaa gacacaggt tttcccacc 
      aatgtcacg tatctttgg ttccgcaac ggtcacatg 
      atacacaac atcacagtt tccaactgg acaattccg 
      cacgcttct ttagaaaca cgaagcaat ttcacttgt 
      attgcattg aacaaaggt ggacaaagc aagccagct 
      tatgatttt gtcaatgtt gaagcacca ccgactgta 
      atcaccaaa gctagtgca acttataaa ggcgtactt 
      tacacctcg caaaatatg tcgctcagc tgccaaatc 
      gaatgtttt ccgagatgt tcaatcaaa tggtacaaa 
      gacggacac gaaattaat ttcgaaaat aacaaactt 
      tactacgtg attaacaca aatcattcg gcagattat 
      aaaacgaac gattttgaa tcgatagaa tcgactctg 
      gtttggaat atgacaaac tggccggga aagcagttg 
      gataaaagt gcacctgac tcgtattac acttgtgct 
      agttacgat ccaccagat agacatcga gttggagcg 
      aatttttca actgccatc ggtgtagaa tatccacct 
      gaaaattta acggtgtca cactctgtt atcaacgta 
      atagaagac gaatctccc attgatgtt aagtgtagt 
      ggagatggt cgaccaaac cttaactac gtgtggaaa 
      cgaaaccaa acatctgaa gcaattgta aaaagtgac 
      actcttaag ctgcccaaa atgtcccga acgaaagca 
      ggctactac acatgtgag gctttcaac agacacggc 
      aataaaaca attcaagtc aaattcaac gtttggtac 
      aaacccgag tgtaaaatc gaacgcggc gaaatcgaa 
      tacaacccg tctttaatt tgcaaagcc gacggaaat 
      ccgaacgaa atgaccttt acctggatg atgatcgag 
      cagaacaaa acttccgag ctgaatccg agtggaggc 
      aacaccaac tttagttat ttctttcta gattcaagc 
      gtaaccacc gatattgag taccaatgt gtggcaaac 
      aattcaatc aaagctagt gatccttgc tctataaaa 
      attccgggg agcaaactg ccttggtat ctcagcaag 
      gaaaacttg atcgtcctg ataacatcg attataaca 
      ctcatcatc tgtttcata atcgtctgc attgtcatc 
      atcgttttg tgtcgaaag aaacgccaa aataccaaa 
      tcacccaac ccgtcctct acaggagat aagacgaga 
      ttaggggct gctccaccg gttggcggt tcagcagac 
      cccacaaat gatcacgac aaaggattt tacgaaaac 
      ctccctttc cacggcatg caaaaccca ccaaataaa 
      ccaatttct atcattgct ccattcagt caagaaatg 
      cgtaatttt actcccaaa acgccacca tcaattaac 
      ccttgccct aaaattact gtacaacgt catcagtcg 
      tttcatggc ttccaaaac actcacttg agatttgat 
      ttcaaccga agcaaccct ttcttgaat aattgtgcc 
      aactttcaa aacaatcca caagttgag acgcccttc 
      agctttcag aataatttc caaccgata ttaaatttt 
      aatccattt ttgcttccg aaccctttc agtgtacca 
      cctcgaaca aatcgagtt gcaggtcga agggaaacg 
      gatgtgaga cgaacggct tccggtatc aatatcgcg 
      gaaaataat caagtcgaa acaacaaaa attgaaaaa 
      tcaatacca aacgataag cgtttcggt tctctagaa 
      ttgaaaaaa cacaaatgt tactcacct acgttctat 
      tcaatgcgg tgtaaaaaa cacgccaaa aaaagaccg 
      gtcgtttat gcgttaccc aaaaaagtt ttcggcgaa 
      cttaagcca agcgaagac aaacaacaa caatttcaa 
      aatcttaca gatagtatt gaaatttcg gaacaaaac 
      tcatcacct aaaactttt ccaattcct gcacctagg 
      tgccgaaag catttgaaa aaagacgtt tccacaaac 
      aattctcca aacccggac gtttctaat agttccaac 
      gaaggttcg gaacatctt gaaagcgaa atttccgtg 
      attgaagca caaattcac acttcttgt aatgagagc 
      gaaaccaaa aattcttcg agtggaata attaaagtg 
      gaagccatg aaagcactc aaaaattct cccatgtta 
      aaagtcagt cccaatttt atcaaaccc aaaactgaa 
      agtcctaaa ggtgccttg tccttgcaa atccaagcc 
      aaaagaaaa aattccaca gacaattta aaccaaaca 
      acagatgat agtagcaaa gagaattta cccttaata 
      ccgcaaatg ccattatta aaccctcag tgtaagtgg 
      agtcctggc ttaactagt gttaaacaa cagaacgct 
      ttttacaca ctaacaacc ccgcattac aaacatgta 
      aatgttggc ttgcccccg caatattca gcgaccatc 
      cccaagcac accaagctc atacctcga gcattattc 
      aatgagcct ttaccgaag agcaaatcc ttttcgtcg 
      aaaccaaaa cacaaaaag cacttccaa ataccccta 
      caaaagtgc cacagtttt aagtttcaa acggcggaa 
      agttacttt caaccgatt cgaaacatc cacgaagaa 
      gatcttatg aagaacggc tacgtgtca gactaccct 
      cctcctagt gaaagtagt agtcgaacg acacgacat 
      cataaacgt ggaggtgaa aaagccaaa cagaaaact 
      aaagggcct ttggtggta atgaggccc aacatgcaa 
      ttccccgaa agtgtacaa agctccgtt cagcttcaa 
      tatccacag cctgtgtta ggaaggagt gtgaagaag 
      ccgaacgga gtggtttac gcagattta gacatgcct 
      aagactaat aataagaag ggctcacaa cacgacaac 
      agctcttgt atgcaaaat aaaacacaa aagtccaaa 
      ccaaagact gaatacgca acgcttagt tttaatgat 
      gttggtcaa gaaattgac gtttaa
    • >TC006983 RB
      atggatgag gacgaagcc ttcgtcaaa ccgtctaat 
      gttgttttg acctacaat ggaaaggaa gtcaacgaa 
      tcaacaagt ttggaagaa aagattgaa ggtgatgac 
      gtggtgttg acgtgcaag gcacctgga ggaaaaccc 
      attcccaca gtgtcttgg tacaatggg acacaactc 
      atgaacaga gcctcttat tcagcgcga agcgatgga 
      aacgcaacc ggaactgga atttccaag ctgcaactg 
      acgctttca aaatatgat ttaaacgca cgctttgaa 
      tgcaaggtt gaaagtcag gctttagaa gaagcgatc 
      gtgaaaggg gtgacgctt cccgttaaa gtgaagccg 
      acgaaaata aagctgtcc ggtgtgaat ggacatgtg 
      aatcaggga acaatcgtt tcacttata tgccaagtt 
      tttggagct aggccgatg gctgaagtc gaatggtac 
      aacagcact tccatcaga atggtcaca aacagcagc 
      agaggaatt gaagttcgt acagtctac gagcaagat 
      gcaggtggt acttacaca acgaaaagc acacttagt 
      tttcctgca acgagattc gaaaatggc aaacgtttt 
      gaatgttac ggcaaaaat gaagtaaca acacttaaa 
      agcgagcca gatttatac gaaccaaaa attctggat 
      gtacgatat ccaccagtg attgaagtt ggtgcgatt 
      ggaagtaca gttgttaac gaaaatgta aatgttact 
      ttaacttgt cgatatttt gcaaatccg caaaaattg 
      gaaggtgca acttggatc aaagatggt aaaaacttg 
      acgaaaatt ccaaacaag cattacgga ggtgatgtg 
      gaaatgcca tctcttact ataataggc gtcacaagg 
      gaagatgct ggtgattat agttgtcta ttacagaac 
      gctgtgaat tcgtcgatt tccgaagac attatttat 
      ttggatgtt cattatcct cctgatgta gaagtcgta 
      atggctccc tcgacacct gccaatgcc actgaacaa 
      acaaatgtt acgcttgta tgtaacgta acatcggga 
      aatccccca gcgctacgt aaagtgcgt tggtttctg 
      gacggtgag cttatggct gaacttccc gaatgcaac 
      tacagccaa ccggtgatc aacgacaca atcggaggc 
      cccttttgt ggcattgat cccagtatt ttaagtttg 
      atcaaagtt gagcagact tttgctgga aactacacc 
      tgtcaaggg gaaaatgaa gctggttct ggacctgtg 
      tctgaaccc cgagagcta atagtttac tatcctcct 
      tctgccgcc aaactcaca cactaccct ccaacggtt 
      atgaaaagt ggccaagtt actttaacg tgcagtgtg 
      gaagacaca ggttttccc accaatgtc acgtatctt 
      tggttccgc aacggtcac atgatacac aacatcaca 
      gtttccaac tggacaatt ccgcacgct tctttagaa 
      acacgaagc aatttcact tgtattgca ttgaacaaa 
      ggtggacaa agcaagcca gcttatgat tttgtcaat 
      gttgaagca ccaccgact gtaatcacc aaagctagt 
      gcaacttat aaaggcgta ctttacacc tcgcaaaat 
      atgtcgctc agctgccaa atcgaatgt tttccgaga 
      tgttcaatc aaatggtac aaagacgga cacgaaatt 
      aatttcgaa aataacaaa ctttactac gtgattaac 
      acaaatcat tcggcagat tataaaacg aacgatttt 
      gaatcgata gaatcgact ctggtttgg aatatgaca 
      aactggccg ggaaagcag ttggataaa agtgcacct 
      gactcgtat tacacttgt gctagttac gatccacca 
      gatagacat cgagttgga gcgaatttt tcaactgcc 
      atcggtgta gaatatcca cctgaaaat ttaacggtg 
      tcacactct gttatcaac gtaatagaa gacgaatct 
      cccattgat gttaagtgt agtggagat ggtcgacca 
      aaccttaac tacgtgtgg aaacgaaac caaacatct 
      gaagcaatt gtaaaaagt gacactctt aagctgccc 
      aaaatgtcc cgaacgaaa gcaggctac tacacatgt 
      gaggctttc aacagacac ggcaataaa acaattcaa 
      gtcaaattc aacgtttgg tacaaaccc gagtgtaaa 
      atcgaacgc ggcgaaatc gaatacaac ccgtcttta 
      atttgcaaa gccgacgga aatccgaac gaaatgacc 
      tttacctgg atgatgatc gagcagaac aaaacttcc 
      gagctgaat ccgagtgga ggcaacacc aactttagt 
      tatttcttt ctagattca agcgtaacc accgatatt 
      gagtaccaa tgtgtggca aacaattca atcaaagct 
      agtgatcct tgctctata aaaattccg gggagcaaa 
      ctgccttgg tatctcagc aaggaaaac ttgatcgtc 
      ctgataaca tcgattata acactcatc atctgtttc 
      ataatcgtc tgcattgtc atcatcgtt ttgtgtcga 
      aagaaacgc caaaatacc aaatcaccc aacccgtcc 
      tctacagga gataagacg agattaggg gctgctcca 
      ccggttggc ggttcagca gaccccaca aatgatcac 
      gacaaagga ttttacgaa aacctccct ttccacggc 
      atgcaaaac ccaccaaat aaaccaatt tctatcatt 
      gctccattc agtcaagaa atgcgtaat tttactccc 
      aaaacgcca ccatcaatt aacccttgc cctaaaatt 
      actgtacaa cgtcatcag tcgtttcat ggcttccaa 
      aacactcac ttgagattt gatttcaac cgaagcaac 
      cctttcttg aataattgt gccaacttt caaaacaat 
      ccacaagtt gagacgccc ttcagcttt cagaataat 
      ttccaaccg atattaaat tttaatcca tttttgctt 
      ccgaaccct ttcagtgta ccacctcga acaaatcga 
      gttgcaggt cgaagggaa acggatgtg agacgaacg 
      gcttccggt atcaatatc gcggaaaat aatcaagtc 
      gaaacaaca aaaattgaa aaatcaata ccaaacgat 
      aagcgtttc ggttctcta gaattgaaa aaacacaaa 
      tgttactca cctacgttc tattcaatg cggtgtaaa 
      aaacacgcc aaaaaaaga ccggtcgtt tatgcgtta 
      cccaaaaaa gttttcggc gaacttaag ccaagcgaa 
      gacaaacaa caacaattt caaaatctt acagatagt 
      attgaaatt tcggaacaa aactcatca cctaaaact 
      tttccaatt cctgcacct aggtgccga aagcatttg 
      aaaaaagac gtttccaca aacaattct ccaaacccg 
      gacgtttct aatagttcc aacgaaggt tcggaacat 
      cttgaaagc gaaatttcc gtgattgaa gcacaaatt 
      cacacttct tgtaatgag agcgaaacc aaaaattct 
      tcgagtgga ataattaaa gtggaagcc atgaaagca 
      ctcaaaaat tctcccatg ttaaaagtc agtcccaat 
      tttatcaaa cccaaaact gaaagtcct aaaggtgcc 
      ttgtccttg caaatccaa gccaaaaga aaaaattcc 
      acagacaat ttaaaccaa acaacagat gatagtagc 
      aaagagaat ttaccctta ataccgcaa atgccatta 
      ttaaaccct cagtgtaag tggagtcct ggcttaact 
      agtgttaaa caacagaac gctttttac acactaaca 
      accccgcat tacaaacat gtaaatgtt ggcttgccc 
      ccgcaatat tcagcgacc atccccaag cacaccaag 
      ctcatacct cgagcatta ttcaatgag cctttaccg 
      aagagcaaa tccttttcg tcgaaacca aaacacaaa 
      aagcacttc caaataccc ctacaaaag tgccacagt 
      tttaagttt caaacggcg gaaagttac tttcaaccg 
      attcgaaac atccacgaa gaagatctt atgaagaac 
      ggctacgtg tcagactac cctcctcct agtgaaagt 
      agtagtcga acgacacga catcataaa cgtggaggt 
      gaaaaagcc aaacagaaa actaaaggg cctttggtg 
      gtaatgagg cccaacatg caattcccc gaaagtgta 
      caaagctcc gttcagctt caatatcca cagcctgtg 
      ttaggaagg agtgtgaag aagccgaac ggagtggtt 
      tacgcagat ttagacatg cctaagact aataataag 
      aagggctca caacacgac aacagctct tgtatgcaa 
      aataaaaca caaaagtcc aaaccaaag actgaatac 
      gcaacgctt agttttaat gatgttggt caagaaatt 
      gacgtttaa
    mRNA sequence
    • >TC006983 RA
      cgttccgtt cgccgccga ttgtagttc acgaccaga 
      aacgaacac gaatcaaac acgaaagct cgccggtgt 
      tatcatgag ctttttccg gaatatttt tcagtgatc 
      cgatttttc agtgatttg cttaaccgt agcattttt 
      cagtcggtc ccttgagct gttattgtg tcaccaact 
      gctaaattt aaacaacat tttaaaata gaacaaact 
      gtcgcaaaa ttaacttct tcaaagaca cgattttgt 
      gtgcaggca tgtgaattt tattaagat aattattgt 
      ggacgaact ttttgggaa atggatgaa atgaaactt 
      ttcggcact tgggtgctt ttgctcggc tttctactg 
      caatataca gcaacgaag gagacggaa gttcgagca 
      cttgttggg acgaatgtg ctgctgcct tgcagagtg 
      gacgtgaac caatgtggc accttgcac agcgtcaaa 
      tggtacaag gatacgtcc aggatatat gtgttcagt 
      caagctggc cagatccga cgggcggaa ggagacgct 
      acagaaaga atgaacatg acgtataat gaagccaaa 
      aatgaaaca tttttacaa ttatctccg gtgaaagta 
      gacgacgaa gcgacttac aaatgcgaa ataacatat 
      ctggaagtg gtggagaac tgtgacgtt attcaaatc 
      gttaaactc acgacacta gtcaaaccg tctaatgtt 
      gttttgacc tacaatgga aaggaagtc aacgaatca 
      acaagtttg gaagaaaag attgaaggt gatgacgtg 
      gtgttgacg tgcaaggca cctggagga aaacccatt 
      cccacagtg tcttggtac aatgggaca caactcatg 
      aacagagcc tcttattca gcgcgaagc gatggaaac 
      gcaaccgga actggaatt tccaagctg caactgacg 
      ctttcaaaa tatgattta aacgcacgc tttgaatgc 
      aaggttgaa agtcaggct ttagaagaa gcgatcgtg 
      aaaggggtg acgcttccc gttaaagtg aagccgacg 
      aaaataaag ctgtccggt gtgaatgga catgtgaat 
      cagggaaca atcgtttca cttatatgc caagttttt 
      ggagctagg ccgatggct gaagtcgaa tggtacaac 
      agcacttcc atcagaatg gtcacaaac agcagcaga 
      ggaattgaa gttcgtaca gtctacgag caagatgca 
      ggtggtact tacacaacg aaaagcaca cttagtttt 
      cctgcaacg agattcgaa aatggcaaa cgttttgaa 
      tgttacggc aaaaatgaa gtaacaaca cttaaaagc 
      gagccagat ttatacgaa ccaaaaatt ctggatgta 
      cgatatcca ccagtgatt gaagttggt gcgattgga 
      agtacagtt gttaacgaa aatgtaaat gttacttta 
      acttgtcga tattttgca aatccgcaa aaattggaa 
      ggtgcaact tggatcaaa gatggtaaa aacttgacg 
      aaaattcca aacaagcat tacggaggt gatgtggaa 
      atgccatct cttactata ataggcgtc acaagggaa 
      gatgctggt gattatagt tgtctatta cagaacgct 
      gtgaattcg tcgatttcc gaagacatt atttatttg 
      gatgttcat tatcctcct gatgtagaa gtcgtaatg 
      gctccctcg acacctgcc aatgccact gaacaaaca 
      aatgttacg cttgtatgt aacgtaaca tcgggaaat 
      cccccagcg ctacgtaaa gtgcgttgg tttctggac 
      ggtgagctt atggctgaa cttcccgaa tgcaactac 
      agccaaccg gtgatcaac gacacaatc ggaggcccc 
      ttttgtggc attgatccc agtatttta agtttgatc 
      aaagttgag cagactttt gctggaaac tacacctgt 
      caaggggaa aatgaagct ggttctgga cctgtgtct 
      gaaccccga gagctaata gtttactat cctccttct 
      gccgccaaa ctcacacac taccctcca acggttatg 
      aaaagtggc caagttact ttaacgtgc agtgtggaa 
      gacacaggt tttcccacc aatgtcacg tatctttgg 
      ttccgcaac ggtcacatg atacacaac atcacagtt 
      tccaactgg acaattccg cacgcttct ttagaaaca 
      cgaagcaat ttcacttgt attgcattg aacaaaggt 
      ggacaaagc aagccagct tatgatttt gtcaatgtt 
      gaagcacca ccgactgta atcaccaaa gctagtgca 
      acttataaa ggcgtactt tacacctcg caaaatatg 
      tcgctcagc tgccaaatc gaatgtttt ccgagatgt 
      tcaatcaaa tggtacaaa gacggacac gaaattaat 
      ttcgaaaat aacaaactt tactacgtg attaacaca 
      aatcattcg gcagattat aaaacgaac gattttgaa 
      tcgatagaa tcgactctg gtttggaat atgacaaac 
      tggccggga aagcagttg gataaaagt gcacctgac 
      tcgtattac acttgtgct agttacgat ccaccagat 
      agacatcga gttggagcg aatttttca actgccatc 
      ggtgtagaa tatccacct gaaaattta acggtgtca 
      cactctgtt atcaacgta atagaagac gaatctccc 
      attgatgtt aagtgtagt ggagatggt cgaccaaac 
      cttaactac gtgtggaaa cgaaaccaa acatctgaa 
      gcaattgta aaaagtgac actcttaag ctgcccaaa 
      atgtcccga acgaaagca ggctactac acatgtgag 
      gctttcaac agacacggc aataaaaca attcaagtc 
      aaattcaac gtttggtac aaacccgag tgtaaaatc 
      gaacgcggc gaaatcgaa tacaacccg tctttaatt 
      tgcaaagcc gacggaaat ccgaacgaa atgaccttt 
      acctggatg atgatcgag cagaacaaa acttccgag 
      ctgaatccg agtggaggc aacaccaac tttagttat 
      ttctttcta gattcaagc gtaaccacc gatattgag 
      taccaatgt gtggcaaac aattcaatc aaagctagt 
      gatccttgc tctataaaa attccgggg agcaaactg 
      ccttggtat ctcagcaag gaaaacttg atcgtcctg 
      ataacatcg attataaca ctcatcatc tgtttcata 
      atcgtctgc attgtcatc atcgttttg tgtcgaaag 
      aaacgccaa aataccaaa tcacccaac ccgtcctct 
      acaggagat aagacgaga ttaggggct gctccaccg 
      gttggcggt tcagcagac cccacaaat gatcacgac 
      aaaggattt tacgaaaac ctccctttc cacggcatg 
      caaaaccca ccaaataaa ccaatttct atcattgct 
      ccattcagt caagaaatg cgtaatttt actcccaaa 
      acgccacca tcaattaac ccttgccct aaaattact 
      gtacaacgt catcagtcg tttcatggc ttccaaaac 
      actcacttg agatttgat ttcaaccga agcaaccct 
      ttcttgaat aattgtgcc aactttcaa aacaatcca 
      caagttgag acgcccttc agctttcag aataatttc 
      caaccgata ttaaatttt aatccattt ttgcttccg 
      aaccctttc agtgtacca cctcgaaca aatcgagtt 
      gcaggtcga agggaaacg gatgtgaga cgaacggct 
      tccggtatc aatatcgcg gaaaataat caagtcgaa 
      acaacaaaa attgaaaaa tcaatacca aacgataag 
      cgtttcggt tctctagaa ttgaaaaaa cacaaatgt 
      tactcacct acgttctat tcaatgcgg tgtaaaaaa 
      cacgccaaa aaaagaccg gtcgtttat gcgttaccc 
      aaaaaagtt ttcggcgaa cttaagcca agcgaagac 
      aaacaacaa caatttcaa aatcttaca gatagtatt 
      gaaatttcg gaacaaaac tcatcacct aaaactttt 
      ccaattcct gcacctagg tgccgaaag catttgaaa 
      aaagacgtt tccacaaac aattctcca aacccggac 
      gtttctaat agttccaac gaaggttcg gaacatctt 
      gaaagcgaa atttccgtg attgaagca caaattcac 
      acttcttgt aatgagagc gaaaccaaa aattcttcg 
      agtggaata attaaagtg gaagccatg aaagcactc 
      aaaaattct cccatgtta aaagtcagt cccaatttt 
      atcaaaccc aaaactgaa agtcctaaa ggtgccttg 
      tccttgcaa atccaagcc aaaagaaaa aattccaca 
      gacaattta aaccaaaca acagatgat agtagcaaa 
      gagaattta cccttaata ccgcaaatg ccattatta 
      aaccctcag tgtaagtgg agtcctggc ttaactagt 
      gttaaacaa cagaacgct ttttacaca ctaacaacc 
      ccgcattac aaacatgta aatgttggc ttgcccccg 
      caatattca gcgaccatc cccaagcac accaagctc 
      atacctcga gcattattc aatgagcct ttaccgaag 
      agcaaatcc ttttcgtcg aaaccaaaa cacaaaaag 
      cacttccaa ataccccta caaaagtgc cacagtttt 
      aagtttcaa acggcggaa agttacttt caaccgatt 
      cgaaacatc cacgaagaa gatcttatg aagaacggc 
      tacgtgtca gactaccct cctcctagt gaaagtagt 
      agtcgaacg acacgacat cataaacgt ggaggtgaa 
      aaagccaaa cagaaaact aaagggcct ttggtggta 
      atgaggccc aacatgcaa ttccccgaa agtgtacaa 
      agctccgtt cagcttcaa tatccacag cctgtgtta 
      ggaaggagt gtgaagaag ccgaacgga gtggtttac 
      gcagattta gacatgcct aagactaat aataagaag 
      ggctcacaa cacgacaac agctcttgt atgcaaaat 
      aaaacacaa aagtccaaa ccaaagact gaatacgca 
      acgcttagt tttaatgat gttggtcaa gaaattgac 
      gtttaacat tcaataatt aggacacgt actaattta 
      tttagttta aggttagtg aatgcttct aacgaatta 
      tcgttgcat cttttaatt aaacaaatt gcataata
    • >TC006983 RB
      atttttaac attttatta cagcaaggc gaccacaac 
      gccagaaaa taatagttt acactcctt gtaaagaat 
      ttagcgaca cctccgtaa ttaaagtta gtaaccgta 
      agcggtcgt cttcgtcac cccttggct tcggccaaa 
      gtcgtggac aaaaaggac gacctgaaa cggtgcgaa 
      cgtcttggc actggaatt taatttcac accacggca 
      ttctctgga catggatga ggacgaagc cttcgtcaa 
      accgtctaa tgttgtttt gacctacaa tggaaagga 
      agtcaacga atcaacaag tttggaaga aaagattga 
      aggtgatga cgtggtgtt gacgtgcaa ggcacctgg 
      aggaaaacc cattcccac agtgtcttg gtacaatgg 
      gacacaact catgaacag agcctctta ttcagcgcg 
      aagcgatgg aaacgcaac cggaactgg aatttccaa 
      gctgcaact gacgctttc aaaatatga tttaaacgc 
      acgctttga atgcaaggt tgaaagtca ggctttaga 
      agaagcgat cgtgaaagg ggtgacgct tcccgttaa 
      agtgaagcc gacgaaaat aaagctgtc cggtgtgaa 
      tggacatgt gaatcaggg aacaatcgt ttcacttat 
      atgccaagt ttttggagc taggccgat ggctgaagt 
      cgaatggta caacagcac ttccatcag aatggtcac 
      aaacagcag cagaggaat tgaagttcg tacagtcta 
      cgagcaaga tgcaggtgg tacttacac aacgaaaag 
      cacacttag ttttcctgc aacgagatt cgaaaatgg 
      caaacgttt tgaatgtta cggcaaaaa tgaagtaac 
      aacacttaa aagcgagcc agatttata cgaaccaaa 
      aattctgga tgtacgata tccaccagt gattgaagt 
      tggtgcgat tggaagtac agttgttaa cgaaaatgt 
      aaatgttac tttaacttg tcgatattt tgcaaatcc 
      gcaaaaatt ggaaggtgc aacttggat caaagatgg 
      taaaaactt gacgaaaat tccaaacaa gcattacgg 
      aggtgatgt ggaaatgcc atctcttac tataatagg 
      cgtcacaag ggaagatgc tggtgatta tagttgtct 
      attacagaa cgctgtgaa ttcgtcgat ttccgaaga 
      cattattta tttggatgt tcattatcc tcctgatgt 
      agaagtcgt aatggctcc ctcgacacc tgccaatgc 
      cactgaaca aacaaatgt tacgcttgt atgtaacgt 
      aacatcggg aaatccccc agcgctacg taaagtgcg 
      ttggtttct ggacggtga gcttatggc tgaacttcc 
      cgaatgcaa ctacagcca accggtgat caacgacac 
      aatcggagg ccccttttg tggcattga tcccagtat 
      tttaagttt gatcaaagt tgagcagac ttttgctgg 
      aaactacac ctgtcaagg ggaaaatga agctggttc 
      tggacctgt gtctgaacc ccgagagct aatagttta 
      ctatcctcc ttctgccgc caaactcac acactaccc 
      tccaacggt tatgaaaag tggccaagt tactttaac 
      gtgcagtgt ggaagacac aggttttcc caccaatgt 
      cacgtatct ttggttccg caacggtca catgataca 
      caacatcac agtttccaa ctggacaat tccgcacgc 
      ttctttaga aacacgaag caatttcac ttgtattgc 
      attgaacaa aggtggaca aagcaagcc agcttatga 
      ttttgtcaa tgttgaagc accaccgac tgtaatcac 
      caaagctag tgcaactta taaaggcgt actttacac 
      ctcgcaaaa tatgtcgct cagctgcca aatcgaatg 
      ttttccgag atgttcaat caaatggta caaagacgg 
      acacgaaat taatttcga aaataacaa actttacta 
      cgtgattaa cacaaatca ttcggcaga ttataaaac 
      gaacgattt tgaatcgat agaatcgac tctggtttg 
      gaatatgac aaactggcc gggaaagca gttggataa 
      aagtgcacc tgactcgta ttacacttg tgctagtta 
      cgatccacc agatagaca tcgagttgg agcgaattt 
      ttcaactgc catcggtgt agaatatcc acctgaaaa 
      tttaacggt gtcacactc tgttatcaa cgtaataga 
      agacgaatc tcccattga tgttaagtg tagtggaga 
      tggtcgacc aaaccttaa ctacgtgtg gaaacgaaa 
      ccaaacatc tgaagcaat tgtaaaaag tgacactct 
      taagctgcc caaaatgtc ccgaacgaa agcaggcta 
      ctacacatg tgaggcttt caacagaca cggcaataa 
      aacaattca agtcaaatt caacgtttg gtacaaacc 
      cgagtgtaa aatcgaacg cggcgaaat cgaatacaa 
      cccgtcttt aatttgcaa agccgacgg aaatccgaa 
      cgaaatgac ctttacctg gatgatgat cgagcagaa 
      caaaacttc cgagctgaa tccgagtgg aggcaacac 
      caactttag ttatttctt tctagattc aagcgtaac 
      caccgatat tgagtacca atgtgtggc aaacaattc 
      aatcaaagc tagtgatcc ttgctctat aaaaattcc 
      ggggagcaa actgccttg gtatctcag caaggaaaa 
      cttgatcgt cctgataac atcgattat aacactcat 
      catctgttt cataatcgt ctgcattgt catcatcgt 
      tttgtgtcg aaagaaacg ccaaaatac caaatcacc 
      caacccgtc ctctacagg agataagac gagattagg 
      ggctgctcc accggttgg cggttcagc agaccccac 
      aaatgatca cgacaaagg attttacga aaacctccc 
      tttccacgg catgcaaaa cccaccaaa taaaccaat 
      ttctatcat tgctccatt cagtcaaga aatgcgtaa 
      ttttactcc caaaacgcc accatcaat taacccttg 
      ccctaaaat tactgtaca acgtcatca gtcgtttca 
      tggcttcca aaacactca cttgagatt tgatttcaa 
      ccgaagcaa ccctttctt gaataattg tgccaactt 
      tcaaaacaa tccacaagt tgagacgcc cttcagctt 
      tcagaataa tttccaacc gatattaaa ttttaatcc 
      atttttgct tccgaaccc tttcagtgt accacctcg 
      aacaaatcg agttgcagg tcgaaggga aacggatgt 
      gagacgaac ggcttccgg tatcaatat cgcggaaaa 
      taatcaagt cgaaacaac aaaaattga aaaatcaat 
      accaaacga taagcgttt cggttctct agaattgaa 
      aaaacacaa atgttactc acctacgtt ctattcaat 
      gcggtgtaa aaaacacgc caaaaaaag accggtcgt 
      ttatgcgtt acccaaaaa agttttcgg cgaacttaa 
      gccaagcga agacaaaca acaacaatt tcaaaatct 
      tacagatag tattgaaat ttcggaaca aaactcatc 
      acctaaaac ttttccaat tcctgcacc taggtgccg 
      aaagcattt gaaaaaaga cgtttccac aaacaattc 
      tccaaaccc ggacgtttc taatagttc caacgaagg 
      ttcggaaca tcttgaaag cgaaatttc cgtgattga 
      agcacaaat tcacacttc ttgtaatga gagcgaaac 
      caaaaattc ttcgagtgg aataattaa agtggaagc 
      catgaaagc actcaaaaa ttctcccat gttaaaagt 
      cagtcccaa ttttatcaa acccaaaac tgaaagtcc 
      taaaggtgc cttgtcctt gcaaatcca agccaaaag 
      aaaaaattc cacagacaa tttaaacca aacaacaga 
      tgatagtag caaagagaa tttaccctt aataccgca 
      aatgccatt attaaaccc tcagtgtaa gtggagtcc 
      tggcttaac tagtgttaa acaacagaa cgcttttta 
      cacactaac aaccccgca ttacaaaca tgtaaatgt 
      tggcttgcc cccgcaata ttcagcgac catccccaa 
      gcacaccaa gctcatacc tcgagcatt attcaatga 
      gcctttacc gaagagcaa atccttttc gtcgaaacc 
      aaaacacaa aaagcactt ccaaatacc cctacaaaa 
      gtgccacag ttttaagtt tcaaacggc ggaaagtta 
      ctttcaacc gattcgaaa catccacga agaagatct 
      tatgaagaa cggctacgt gtcagacta ccctcctcc 
      tagtgaaag tagtagtcg aacgacacg acatcataa 
      acgtggagg tgaaaaagc caaacagaa aactaaagg 
      gcctttggt ggtaatgag gcccaacat gcaattccc 
      cgaaagtgt acaaagctc cgttcagct tcaatatcc 
      acagcctgt gttaggaag gagtgtgaa gaagccgaa 
      cggagtggt ttacgcaga tttagacat gcctaagac 
      taataataa gaagggctc acaacacga caacagctc 
      ttgtatgca aaataaaac acaaaagtc caaaccaaa 
      gactgaata cgcaacgct tagttttaa tgatgttgg 
      tcaagaaat tgacgttta acattcaat aattaggac 
      acgtactaa tttatttag tttaaggtt agtgaatgc 
      ttctaacga attatcgtt gcatctttt aattaaaca 
      aattgcata ata
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