Status
Please wait ...
Gene information
General information
TC006940 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 225150..242491
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006940
    iBeetle screen iB_04242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006940 RA
      MVESCPRSS MKQINNLMV EESYSLNLY PASVGALAM 
      IGHAQQEPL EGARALFKK SHTCGSLYA KCGCSHDPP 
      PPPVQPRAP EPPPSPSKR GSTPAIASI YPRADTKRL 
      SNPEINNSS RHKTVIKAN VYLNHPEIT FPIPVELQD 
      KPNLAKSLP TSIDLDEVK KSNLDYHKY NVLAYECSA 
      SKEEEAKKF LSDFGIFGP MGNGVATLR RSSKSSLDG 
      DVIQQIRKN SRRLSGAHE ENDYLKTIV ARLKKDLAE 
      LEVKYGSLQ SELYETRRQ LTCKESEVL KLQREIHKL 
      KSVLQQTTT FIQDGDILT SLQEKHAMA NRAPQSQQS 
      LTNSCVLKK QGVSAESSE STGQASDIT IPKHDKDFR 
      SKQLIKDAI MDNNFLQNL DTSQVREIV DCMYLKEYP 
      QGSYVIKEG EAGAHLFVS AEGDFEVMK EDKVLGHMG 
      PGKAFGELA ILYNCTRTA SIRVVVDSK VWVLDRKVF 
      QQIMVRTGL QRLQDNINF LRSVPLLQN LSNEVLAKI 
      ADVLEVEFY PAGVYIIRQ GANGDTFFI ISSGSVKVT 
      QRKQGSMED EEEIRILQR GDYFGEQAL LKEDCRTAN 
      IIAMNPGVE CLTLDRESF KQLIGDLSE IKEKNYGDE 
      QRLNRQINS TCAENSEYD YISLDDLEV IATLGIGGF 
      GRVELVQYV HNPSLTFAL KCLKKQHIV DTQQQDHIF 
      SERNIMMSC RSSFICRMY KTFKDSKYV YMLLEACLG 
      GEVWTILRD RGCFDEDTT KFITGCVLE AFDYLHSRG 
      IIYRDLKPE NLLLDANGY VKLVDFGFS KRLGYSSKT 
      WTFCGTPEY VAPETILNK GHDRAVDYW ALGILMHEL 
      LTGNPPFTA SDPMKTYNL ILKGIDMID FAKHHIGRA 
      AQSLIKKLC RDAPSERLG YQKGGIQDI KKHKWFQGF 
      DWDGLVNQT LPSPIRQPV RGCCDTTNF DCFGKDNDI 
      PPDELSNWD LEF
    CDS sequence
    • >TC006940 RA
      atggtcgaa tcctgtccg agatcctcg atgaagcag 
      ataaataac ttgatggtg gaagaatcg tacagtttg 
      aatttgtat cccgcctca gtcggcgcc ctagcaatg 
      atcggccat gcgcagcag gagcccttg gagggggcc 
      cgcgccctc ttcaaaaaa tcgcacaca tgcggcagc 
      ctctacgcc aagtgcggc tgcagccat gaccccccg 
      ccgcccccc gtccagccg cgcgccccc gagccgccc 
      cccagcccc tcgaagagg ggctccacg cccgccatc 
      gcctccatc tacccgcga gccgacacc aaacgcctc 
      tccaacccg gagatcaac aacagctcg cgccacaag 
      accgtcatc aaagccaac gtctacctg aaccacccc 
      gagatcacg ttccccatc ccggtggag ctccaggac 
      aagcccaac ctcgccaag tcgctgccc accagcatc 
      gacctggac gaagtcaaa aagtccaac ctagactac 
      cacaagtac aacgtgctg gcctacgaa tgcagcgcc 
      agtaaggag gaagaagcg aagaagttc ctcagcgat 
      ttcggaatc ttcgggccg atgggcaac ggtgtcgcg 
      acccttcgg cgaagctcc aaaagctcg ctcgacggc 
      gacgtcatc cagcaaatc cggaaaaac tcgcgccga 
      ctttccgga gcgcacgaa gagaacgac tacttgaaa 
      acaatcgtc gcgcgatta aaaaaagat cttgcggaa 
      ctggaagtc aagtatgga tcgctacaa tcggaactg 
      tacgaaacc aggaggcag ctcacgtgc aaagagagc 
      gaagtgctc aaattgcaa cgcgaaatt cataagctc 
      aagagcgta ctccagcaa accacaaca tttattcag 
      gatggcgat attctcacg agcctccag gaaaagcac 
      gcgatggct aaccgagct ccccaaagt caacaatct 
      ctcaccaac agctgtgtt ttaaagaaa cagggtgta 
      tcagccgaa tcgagcgaa tccaccggt caagcaagc 
      gacattacc attccgaaa catgataag gacttcagg 
      tccaaacag cttatcaaa gacgcgatc atggacaac 
      aactttttg caaaatttg gacacgtca caagtgcga 
      gaaatcgtc gactgcatg tacctcaag gagtatccg 
      cagggttct tatgtgatc aaggaagga gaagctgga 
      gcgcattta tttgtctca gctgagggt gattttgag 
      gtgatgaaa gaagataag gttttgggg cacatgggg 
      ccagggaaa gccttcgga gagttggcg attttgtat 
      aattgtacc cgaactgct tcaattcga gtggttgtt 
      gactcaaaa gtctgggtt ttggaccga aaggtcttc 
      caacaaatc atggtccga actggcctt cagagactc 
      caagacaat atcaatttt ctccgctct gtgcctctc 
      ctccagaat ctcagcaac gaagttttg gcaaaaata 
      gcagacgtc ctcgaagtc gaattttat cctgcaggt 
      gtttacatt ataagacaa ggggccaac ggtgacacc 
      ttctttatc ataagcagc ggctccgtt aaagtaacg 
      caacggaaa cagggctcc atggaagat gaggaagaa 
      atcaggatt ctccaacgt ggcgattac ttcggcgaa 
      caagcttta ctaaaagaa gactgcagg actgccaac 
      ataattgca atgaatcca ggggttgaa tgcttgaca 
      cttgaccgg gaatctttc aagcaacta atcggagat 
      ctgagcgaa atcaaggaa aaaaactac ggtgatgaa 
      caaagactt aaccgccaa atcaactca acgtgtgcc 
      gaaaactcg gaatatgac tacatcagt ttggacgat 
      cttgaagtg attgcaacg ctcggtatt ggaggtttt 
      ggtcgagtt gaactagtt caatacgtt cacaatccg 
      tcactaact ttcgctctc aagtgcctc aagaagcaa 
      catatcgtg gacacgcaa cagcaggac cacatcttc 
      agcgaacgc aacatcatg atgagctgt cggagcagt 
      ttcatctgc aggatgtac aaaacgttc aaggattcc 
      aaatatgtg tacatgttg ctggaggcg tgtctgggt 
      ggcgaagtt tggacgatt ctacgagat cgaggatgt 
      ttcgatgaa gacacgacc aaatttatc accgggtgc 
      gttttggaa gcgtttgat tatctacac agtagaggg 
      ataatctac agggacctc aaacccgaa aatctgctc 
      ttggacgcc aatggatac gtgaaactt gttgatttt 
      gggttttcg aaacggctg gggtacagt agcaagact 
      tggactttt tgcggaacg cctgaatat gtagcgccg 
      gaaactatc ctcaacaag ggacatgac cgagctgtg 
      gactactgg gctcttgga attttaatg cacgaactt 
      ttaaccgga aatcccccg tttacggct tcagacccc 
      atgaaaacc tacaatctc atactgaaa ggcatagat 
      atgatcgat tttgccaaa catcatatc gggagagcg 
      gcacaaagt cttatcaaa aaattgtgt cgggatgct 
      ccatcggag aggttgggg tatcagaaa ggtggaata 
      caagatatt aaaaaacat aaatggttt caaggattt 
      gactgggat ggtttagtc aatcaaacg ctaccttca 
      ccaattaga caaccagtg agaggttgt tgcgataca 
      accaatttt gactgcttt ggcaaagat aacgacatt 
      cctccagat gagttatca aactgggat ttggaattt 
      taa
    mRNA sequence
    • >TC006940 RA
      agtgtctgt gcgattttt ttgaggatt ttccaaacc 
      ataaatcca gccaaatgt ccaccgacc ttttcccag 
      tgtgacgaa aatacaaag ttttcctaa tggtcgaat 
      cctgtccga gatcctcga tgaagcaga taaataact 
      tgatggtgg aagaatcgt acagtttga atttgtatc 
      ccgcctcag tcggcgccc tagcaatga tcggccatg 
      cgcagcagg agcccttgg agggggccc gcgccctct 
      tcaaaaaat cgcacacat gcggcagcc tctacgcca 
      agtgcggct gcagccatg accccccgc cgccccccg 
      tccagccgc gcgcccccg agccgcccc ccagcccct 
      cgaagaggg gctccacgc ccgccatcg cctccatct 
      acccgcgag ccgacacca aacgcctct ccaacccgg 
      agatcaaca acagctcgc gccacaaga ccgtcatca 
      aagccaacg tctacctga accaccccg agatcacgt 
      tccccatcc cggtggagc tccaggaca agcccaacc 
      tcgccaagt cgctgccca ccagcatcg acctggacg 
      aagtcaaaa agtccaacc tagactacc acaagtaca 
      acgtgctgg cctacgaat gcagcgcca gtaaggagg 
      aagaagcga agaagttcc tcagcgatt tcggaatct 
      tcgggccga tgggcaacg gtgtcgcga cccttcggc 
      gaagctcca aaagctcgc tcgacggcg acgtcatcc 
      agcaaatcc ggaaaaact cgcgccgac tttccggag 
      cgcacgaag agaacgact acttgaaaa caatcgtcg 
      cgcgattaa aaaaagatc ttgcggaac tggaagtca 
      agtatggat cgctacaat cggaactgt acgaaacca 
      ggaggcagc tcacgtgca aagagagcg aagtgctca 
      aattgcaac gcgaaattc ataagctca agagcgtac 
      tccagcaaa ccacaacat ttattcagg atggcgata 
      ttctcacga gcctccagg aaaagcacg cgatggcta 
      accgagctc cccaaagtc aacaatctc tcaccaaca 
      gctgtgttt taaagaaac agggtgtat cagccgaat 
      cgagcgaat ccaccggtc aagcaagcg acattacca 
      ttccgaaac atgataagg acttcaggt ccaaacagc 
      ttatcaaag acgcgatca tggacaaca actttttgc 
      aaaatttgg acacgtcac aagtgcgag aaatcgtcg 
      actgcatgt acctcaagg agtatccgc agggttctt 
      atgtgatca aggaaggag aagctggag cgcatttat 
      ttgtctcag ctgagggtg attttgagg tgatgaaag 
      aagataagg ttttggggc acatggggc cagggaaag 
      ccttcggag agttggcga ttttgtata attgtaccc 
      gaactgctt caattcgag tggttgttg actcaaaag 
      tctgggttt tggaccgaa aggtcttcc aacaaatca 
      tggtccgaa ctggccttc agagactcc aagacaata 
      tcaattttc tccgctctg tgcctctcc tccagaatc 
      tcagcaacg aagttttgg caaaaatag cagacgtcc 
      tcgaagtcg aattttatc ctgcaggtg tttacatta 
      taagacaag gggccaacg gtgacacct tctttatca 
      taagcagcg gctccgtta aagtaacgc aacggaaac 
      agggctcca tggaagatg aggaagaaa tcaggattc 
      tccaacgtg gcgattact tcggcgaac aagctttac 
      taaaagaag actgcagga ctgccaaca taattgcaa 
      tgaatccag gggttgaat gcttgacac ttgaccggg 
      aatctttca agcaactaa tcggagatc tgagcgaaa 
      tcaaggaaa aaaactacg gtgatgaac aaagactta 
      accgccaaa tcaactcaa cgtgtgccg aaaactcgg 
      aatatgact acatcagtt tggacgatc ttgaagtga 
      ttgcaacgc tcggtattg gaggttttg gtcgagttg 
      aactagttc aatacgttc acaatccgt cactaactt 
      tcgctctca agtgcctca agaagcaac atatcgtgg 
      acacgcaac agcaggacc acatcttca gcgaacgca 
      acatcatga tgagctgtc ggagcagtt tcatctgca 
      ggatgtaca aaacgttca aggattcca aatatgtgt 
      acatgttgc tggaggcgt gtctgggtg gcgaagttt 
      ggacgattc tacgagatc gaggatgtt tcgatgaag 
      acacgacca aatttatca ccgggtgcg ttttggaag 
      cgtttgatt atctacaca gtagaggga taatctaca 
      gggacctca aacccgaaa atctgctct tggacgcca 
      atggatacg tgaaacttg ttgattttg ggttttcga 
      aacggctgg ggtacagta gcaagactt ggacttttt 
      gcggaacgc ctgaatatg tagcgccgg aaactatcc 
      tcaacaagg gacatgacc gagctgtgg actactggg 
      ctcttggaa ttttaatgc acgaacttt taaccggaa 
      atcccccgt ttacggctt cagacccca tgaaaacct 
      acaatctca tactgaaag gcatagata tgatcgatt 
      ttgccaaac atcatatcg ggagagcgg cacaaagtc 
      ttatcaaaa aattgtgtc gggatgctc catcggaga 
      ggttggggt atcagaaag gtggaatac aagatatta 
      aaaaacata aatggtttc aaggatttg actgggatg 
      gtttagtca atcaaacgc taccttcac caattagac 
      aaccagtga gaggttgtt gcgatacaa ccaattttg 
      actgctttg gcaaagata acgacattc ctccagatg 
      agttatcaa actgggatt tggaatttt aacacaaat 
      acgatgttt ttataactg aaatggaca ataattgat 
      gttaacgta a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY