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Gene information
General information
TC006929 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451772.1: 13438..16394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC006929
      iBeetle screen iB_00976

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection

      iBeetle screen iB_04239

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
      ovary without detectable defects - Number of animals showing the phenotype: 2
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006929 RA
        MENERRLRS KGPVKHSPK KPLNLPTSL PSSANSPKT 
LPQNKPIPS LMDKKILPP PQTHATTLK STKHTQQRS 
RTPSPSNSE TSASFSSTT SEKSSSTTT SKKLSFRYS 
IQNIPAPLA TQKSFYSHL ISLGLHQID SLKVNYNKS 
ALLVLSRQL TPNQISTLQ AGFPGSKIQ FKLLNPTRL 
LPQIEPKPI TFSIVVILL KHLPDTAIT SITTIYNSC 
FNSGHFPTP WKEATTIMI PKPNKDPNN PLSYRPISL 
LNILGKIFE TILASRLRH TLEENNLLP PEQFGFRPH 
RSTSDPTFE LFTDSTRSA NLGQCTLAV FLDVERAFD 
RVWHDGLIQ KFLSHNINL NFIKLIDSF LSNRSCKVK 
VQNSFSYPV PLLSGVPQG SVLSPILYI FYSSDFPVS 
DLHQTKTRF FADDTALWT SRTTAASAS RTLQPLLNQ 
ISTWTRKWR VKINPTKST AILFKHPNL TRNKKFDPR 
DITITLDNT PIDLVPSTR YLGITYTHT CSLDTDLQN 
TLKKVRNRS NLLRHLMGN FQGCHSRTL LHTYNSFIR 
PVIEYRAPI YASLPHTKT HRIATCERQ ILRRIHRLD 
RFHASNSLH QDTNTTPIT DRLHKLQQN YIKRTLNNN 
NTIAQNTLN TSHKFLTRD NTLRNRVPR IPRRKLPHP 
PTALLSALY PDLPDNLQL LVEETPLTM RRH
      CDS sequence
      • >TC006929 RA
        atggagaac gagcgtcgg ctccgctcc aagggcccg 
gtgaaacac tcaccaaaa aaacccctc aacctaccc 
acttcactt ccgtcttct gcaaactct ccaaaaacg 
cttccccaa aacaaaccc attccgtcc cttatggac 
aaaaaaatt ttgccaccc ccacaaact cacgccact 
acattaaaa tccacaaaa cacacccaa caacgttca 
cgcacaccc tcaccatca aactccgaa acatcagca 
tccttttcg tctaccact tcggaaaaa tcgtcttca 
actaccact tcaaaaaaa ctctcattt cgctactca 
atccaaaac atccctgcc ccactcgcc actcaaaaa 
agcttttac tctcacctc atttcactc ggcctccac 
caaattgac tctctaaaa gtcaattac aacaaatcc 
gctcttctt gttctttcc aggcaactt acccctaac 
caaatttca acgcttcaa gctggcttc cctggctcg 
aaaatccag ttcaaactc ttaaacccc acacgcctc 
ctaccccaa atcgaaccc aaacccatc actttttcc 
atcgttgtc attcttctc aaacatctc cctgacaca 
gccatcaca tccatcacc acaatttac aattcttgc 
ttcaattcc ggtcacttt ccaacccca tggaaagaa 
gccaccacc atcatgatt cccaagccc aacaaagac 
cccaacaac ccactttct tatcgtccc atctctctc 
ctcaacatt ctgggaaaa atcttcgaa acaattctt 
gcctctcgt ctccgacac actctcgaa gaaaacaac 
ctactgccc cctgaacaa ttcggtttt cgcccccat 
cgctccact tccgacccc actttcgaa ctcttcacc 
gactcaacc cggagtgcc aatctcggc cagtgcaca 
ctagccgtc ttcttggac gtcgaacgt gcatttgac 
cgtgtttgg cacgatggt ctcatccaa aaatttctt 
tcccacaac atcaacctc aacttcatc aaattaatc 
gactctttt ctctcaaac cgctcctgc aaagtcaaa 
gtccaaaat tccttttct tatcccgtc ccccttctc 
tccggggtc cctcaaggc tccgttctg tcgcccatt 
ctttacatt ttttacagt tctgatttt cctgtttca 
gatcttcac cagaccaaa acccgcttc ttcgccgac 
gacaccgcc ctctggaca tcccggacc accgccgcc 
tcagcctcc aggaccctg cagcccctc ctcaaccag 
atctccaca tggactcgc aagtggaga gtgaagatc 
aaccccaca aaatccacc gccatcctc ttcaaacac 
cccaacctc acacgaaac aagaaattc gacccacgc 
gacatcacc atcacactc gacaacaca cccatcgac 
cttgtgcca tccacaagg tacctagga atcacctac 
acacacaca tgctcactc gacaccgac ctccagaac 
acgctcaag aaagtccgc aaccgatcc aacctcctc 
cgccatctc atgggaaac ttccaaggg tgccattcc 
cgcactctt ctccacacc tacaactca ttcatccga 
cccgtcatc gagtaccga gcccccatc tacgcatct 
ctcccacac accaagaca catcgcatt gccacatgc 
gaacgacaa atccttcgc cgaattcac cgcctcgac 
cgcttccac gccagcaat tccctccac caggacaca 
aacaccacc cccatcaca gaccgtctt cacaaactc 
caacaaaac tacatcaaa cgcacactc aacaacaac 
aacacaatt gcccaaaac acactcaac acttcacat 
aagtttctc acacgcgac aacactctt cgcaaccga 
gtcccccgg atccccaga agaaaactg ccccatcca 
cccacggcg cttctctca gcgctgtac cccgacctg 
cccgacaat cttcaactt ctcgttgaa gagacgccc 
ctcacaatg agacgccac tga
      mRNA sequence
      • >TC006929 RA
        gttgttttc gacgaagca gtcaaatct gtgtcagtt 
cctttcgcg ttctcgagg tgaacacat cgagtctct 
gcgctgact ctgatcgct cagccactc gattgtttg 
ctaagtgaa gtgacagtg aaatccaaa gtgataaat 
tgcaaaagt ttccacaat ggagaacga gcgtcggct 
ccgctccaa gggcccggt gaaacactc accaaaaaa 
acccctcaa cctacccac ttcacttcc gtcttctgc 
aaactctcc aaaaacgct tccccaaaa caaacccat 
tccgtccct tatggacaa aaaaatttt gccaccccc 
acaaactca cgccactac attaaaatc cacaaaaca 
cacccaaca acgttcacg cacaccctc accatcaaa 
ctccgaaac atcagcatc cttttcgtc taccacttc 
ggaaaaatc gtcttcaac taccacttc aaaaaaact 
ctcatttcg ctactcaat ccaaaacat ccctgcccc 
actcgccac tcaaaaaag cttttactc tcacctcat 
ttcactcgg cctccacca aattgactc tctaaaagt 
caattacaa caaatccgc tcttcttgt tctttccag 
gcaacttac ccctaacca aatttcaac gcttcaagc 
tggcttccc tggctcgaa aatccagtt caaactctt 
aaaccccac acgcctcct accccaaat cgaacccaa 
acccatcac tttttccat cgttgtcat tcttctcaa 
acatctccc tgacacagc catcacatc catcaccac 
aatttacaa ttcttgctt caattccgg tcactttcc 
aaccccatg gaaagaagc caccaccat catgattcc 
caagcccaa caaagaccc caacaaccc actttctta 
tcgtcccat ctctctcct caacattct gggaaaaat 
cttcgaaac aattcttgc ctctcgtct ccgacacac 
tctcgaaga aaacaacct actgccccc tgaacaatt 
cggttttcg cccccatcg ctccacttc cgaccccac 
tttcgaact cttcaccga ctcaacccg gagtgccaa 
tctcggcca gtgcacact agccgtctt cttggacgt 
cgaacgtgc atttgaccg tgtttggca cgatggtct 
catccaaaa atttctttc ccacaacat caacctcaa 
cttcatcaa attaatcga ctcttttct ctcaaaccg 
ctcctgcaa agtcaaagt ccaaaattc cttttctta 
tcccgtccc ccttctctc cggggtccc tcaaggctc 
cgttctgtc gcccattct ttacatttt ttacagttc 
tgattttcc tgtttcaga tcttcacca gaccaaaac 
ccgcttctt cgccgacga caccgccct ctggacatc 
ccggaccac cgccgcctc agcctccag gaccctgca 
gcccctcct caaccagat ctccacatg gactcgcaa 
gtggagagt gaagatcaa ccccacaaa atccaccgc 
catcctctt caaacaccc caacctcac acgaaacaa 
gaaattcga cccacgcga catcaccat cacactcga 
caacacacc catcgacct tgtgccatc cacaaggta 
cctaggaat cacctacac acacacatg ctcactcga 
caccgacct ccagaacac gctcaagaa agtccgcaa 
ccgatccaa cctcctccg ccatctcat gggaaactt 
ccaagggtg ccattcccg cactcttct ccacaccta 
caactcatt catccgacc cgtcatcga gtaccgagc 
ccccatcta cgcatctct cccacacac caagacaca 
tcgcattgc cacatgcga acgacaaat ccttcgccg 
aattcaccg cctcgaccg cttccacgc cagcaattc 
cctccacca ggacacaaa caccacccc catcacaga 
ccgtcttca caaactcca acaaaacta catcaaacg 
cacactcaa caacaacaa cacaattgc ccaaaacac 
actcaacac ttcacataa gtttctcac acgcgacaa 
cactcttcg caaccgagt cccccggat ccccagaag 
aaaactgcc ccatccacc cacggcgct tctctcagc 
gctgtaccc cgacctgcc cgacaatct tcaacttct 
cgttgaaga gacgcccct cacaatgag acgccactg 
acgacaccg acaagacac ccacgacga catccacat 
ccacagctt cctacgagc cccaaactt ctcactctt 
tcactcacc acccgacga cacacacac tcccacaca 
acattcatc catttcctt gactgaaga ggccaaaag 
cctaaaaca gtccggact cgtagatta agttgtaaa 
tatcttgta tatatttta tcaaaatcc cttttgaaa 
tgtaattta ttttgaata aacgtattt tgaattgaa 
ttgaatttg aattagtgc accatgatt ttagcttga 
actttcaag ttgacaggt atattttgt gttcgttgt 
gcgtattac agttcgttt gtgtcgtgt ttttttttt 
ctttattaa gattagaaa tattaaaaa atatgccac 
caaagacaa gcggtaaag cagcgaaaa aggctggaa 
aagctcaaa agaatattt cgaagagcg acaagaaaa 
agaagcgca ggaggaagg aaagctatg caatctata 
tttataagg tattgaagc aagtacacc ccgataccg 
gtatttcga gcaaggcga tgagcatca tgaacagtt 
tcgttaatg atatctttg aacggatcg ccgctgaag 
cttctcgtc ttgcgcatt acaacaagc gttcgacga 
ttacgagcc gggaaattc aaacagcgg ttcgtctct 
tgttgcctg gtgaattgg caaagcacg cagtctctg 
aaggtacca aagccgtca ccaaataca caagtatat 
ataaa
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