Status
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Gene information
General information
TC006929 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451772.1: 13438..16394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006929
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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        Quotation: {{ goTerm.quotation }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_00976

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      iBeetle screen iB_04239

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
      ovary without detectable defects - Number of animals showing the phenotype: 2
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006929 RA
        MENERRLRS KGPVKHSPK KPLNLPTSL PSSANSPKT 
        LPQNKPIPS LMDKKILPP PQTHATTLK STKHTQQRS 
        RTPSPSNSE TSASFSSTT SEKSSSTTT SKKLSFRYS 
        IQNIPAPLA TQKSFYSHL ISLGLHQID SLKVNYNKS 
        ALLVLSRQL TPNQISTLQ AGFPGSKIQ FKLLNPTRL 
        LPQIEPKPI TFSIVVILL KHLPDTAIT SITTIYNSC 
        FNSGHFPTP WKEATTIMI PKPNKDPNN PLSYRPISL 
        LNILGKIFE TILASRLRH TLEENNLLP PEQFGFRPH 
        RSTSDPTFE LFTDSTRSA NLGQCTLAV FLDVERAFD 
        RVWHDGLIQ KFLSHNINL NFIKLIDSF LSNRSCKVK 
        VQNSFSYPV PLLSGVPQG SVLSPILYI FYSSDFPVS 
        DLHQTKTRF FADDTALWT SRTTAASAS RTLQPLLNQ 
        ISTWTRKWR VKINPTKST AILFKHPNL TRNKKFDPR 
        DITITLDNT PIDLVPSTR YLGITYTHT CSLDTDLQN 
        TLKKVRNRS NLLRHLMGN FQGCHSRTL LHTYNSFIR 
        PVIEYRAPI YASLPHTKT HRIATCERQ ILRRIHRLD 
        RFHASNSLH QDTNTTPIT DRLHKLQQN YIKRTLNNN 
        NTIAQNTLN TSHKFLTRD NTLRNRVPR IPRRKLPHP 
        PTALLSALY PDLPDNLQL LVEETPLTM RRH
      CDS sequence
      • >TC006929 RA
        atggagaac gagcgtcgg ctccgctcc aagggcccg 
        gtgaaacac tcaccaaaa aaacccctc aacctaccc 
        acttcactt ccgtcttct gcaaactct ccaaaaacg 
        cttccccaa aacaaaccc attccgtcc cttatggac 
        aaaaaaatt ttgccaccc ccacaaact cacgccact 
        acattaaaa tccacaaaa cacacccaa caacgttca 
        cgcacaccc tcaccatca aactccgaa acatcagca 
        tccttttcg tctaccact tcggaaaaa tcgtcttca 
        actaccact tcaaaaaaa ctctcattt cgctactca 
        atccaaaac atccctgcc ccactcgcc actcaaaaa 
        agcttttac tctcacctc atttcactc ggcctccac 
        caaattgac tctctaaaa gtcaattac aacaaatcc 
        gctcttctt gttctttcc aggcaactt acccctaac 
        caaatttca acgcttcaa gctggcttc cctggctcg 
        aaaatccag ttcaaactc ttaaacccc acacgcctc 
        ctaccccaa atcgaaccc aaacccatc actttttcc 
        atcgttgtc attcttctc aaacatctc cctgacaca 
        gccatcaca tccatcacc acaatttac aattcttgc 
        ttcaattcc ggtcacttt ccaacccca tggaaagaa 
        gccaccacc atcatgatt cccaagccc aacaaagac 
        cccaacaac ccactttct tatcgtccc atctctctc 
        ctcaacatt ctgggaaaa atcttcgaa acaattctt 
        gcctctcgt ctccgacac actctcgaa gaaaacaac 
        ctactgccc cctgaacaa ttcggtttt cgcccccat 
        cgctccact tccgacccc actttcgaa ctcttcacc 
        gactcaacc cggagtgcc aatctcggc cagtgcaca 
        ctagccgtc ttcttggac gtcgaacgt gcatttgac 
        cgtgtttgg cacgatggt ctcatccaa aaatttctt 
        tcccacaac atcaacctc aacttcatc aaattaatc 
        gactctttt ctctcaaac cgctcctgc aaagtcaaa 
        gtccaaaat tccttttct tatcccgtc ccccttctc 
        tccggggtc cctcaaggc tccgttctg tcgcccatt 
        ctttacatt ttttacagt tctgatttt cctgtttca 
        gatcttcac cagaccaaa acccgcttc ttcgccgac 
        gacaccgcc ctctggaca tcccggacc accgccgcc 
        tcagcctcc aggaccctg cagcccctc ctcaaccag 
        atctccaca tggactcgc aagtggaga gtgaagatc 
        aaccccaca aaatccacc gccatcctc ttcaaacac 
        cccaacctc acacgaaac aagaaattc gacccacgc 
        gacatcacc atcacactc gacaacaca cccatcgac 
        cttgtgcca tccacaagg tacctagga atcacctac 
        acacacaca tgctcactc gacaccgac ctccagaac 
        acgctcaag aaagtccgc aaccgatcc aacctcctc 
        cgccatctc atgggaaac ttccaaggg tgccattcc 
        cgcactctt ctccacacc tacaactca ttcatccga 
        cccgtcatc gagtaccga gcccccatc tacgcatct 
        ctcccacac accaagaca catcgcatt gccacatgc 
        gaacgacaa atccttcgc cgaattcac cgcctcgac 
        cgcttccac gccagcaat tccctccac caggacaca 
        aacaccacc cccatcaca gaccgtctt cacaaactc 
        caacaaaac tacatcaaa cgcacactc aacaacaac 
        aacacaatt gcccaaaac acactcaac acttcacat 
        aagtttctc acacgcgac aacactctt cgcaaccga 
        gtcccccgg atccccaga agaaaactg ccccatcca 
        cccacggcg cttctctca gcgctgtac cccgacctg 
        cccgacaat cttcaactt ctcgttgaa gagacgccc 
        ctcacaatg agacgccac tga
      mRNA sequence
      • >TC006929 RA
        gttgttttc gacgaagca gtcaaatct gtgtcagtt 
        cctttcgcg ttctcgagg tgaacacat cgagtctct 
        gcgctgact ctgatcgct cagccactc gattgtttg 
        ctaagtgaa gtgacagtg aaatccaaa gtgataaat 
        tgcaaaagt ttccacaat ggagaacga gcgtcggct 
        ccgctccaa gggcccggt gaaacactc accaaaaaa 
        acccctcaa cctacccac ttcacttcc gtcttctgc 
        aaactctcc aaaaacgct tccccaaaa caaacccat 
        tccgtccct tatggacaa aaaaatttt gccaccccc 
        acaaactca cgccactac attaaaatc cacaaaaca 
        cacccaaca acgttcacg cacaccctc accatcaaa 
        ctccgaaac atcagcatc cttttcgtc taccacttc 
        ggaaaaatc gtcttcaac taccacttc aaaaaaact 
        ctcatttcg ctactcaat ccaaaacat ccctgcccc 
        actcgccac tcaaaaaag cttttactc tcacctcat 
        ttcactcgg cctccacca aattgactc tctaaaagt 
        caattacaa caaatccgc tcttcttgt tctttccag 
        gcaacttac ccctaacca aatttcaac gcttcaagc 
        tggcttccc tggctcgaa aatccagtt caaactctt 
        aaaccccac acgcctcct accccaaat cgaacccaa 
        acccatcac tttttccat cgttgtcat tcttctcaa 
        acatctccc tgacacagc catcacatc catcaccac 
        aatttacaa ttcttgctt caattccgg tcactttcc 
        aaccccatg gaaagaagc caccaccat catgattcc 
        caagcccaa caaagaccc caacaaccc actttctta 
        tcgtcccat ctctctcct caacattct gggaaaaat 
        cttcgaaac aattcttgc ctctcgtct ccgacacac 
        tctcgaaga aaacaacct actgccccc tgaacaatt 
        cggttttcg cccccatcg ctccacttc cgaccccac 
        tttcgaact cttcaccga ctcaacccg gagtgccaa 
        tctcggcca gtgcacact agccgtctt cttggacgt 
        cgaacgtgc atttgaccg tgtttggca cgatggtct 
        catccaaaa atttctttc ccacaacat caacctcaa 
        cttcatcaa attaatcga ctcttttct ctcaaaccg 
        ctcctgcaa agtcaaagt ccaaaattc cttttctta 
        tcccgtccc ccttctctc cggggtccc tcaaggctc 
        cgttctgtc gcccattct ttacatttt ttacagttc 
        tgattttcc tgtttcaga tcttcacca gaccaaaac 
        ccgcttctt cgccgacga caccgccct ctggacatc 
        ccggaccac cgccgcctc agcctccag gaccctgca 
        gcccctcct caaccagat ctccacatg gactcgcaa 
        gtggagagt gaagatcaa ccccacaaa atccaccgc 
        catcctctt caaacaccc caacctcac acgaaacaa 
        gaaattcga cccacgcga catcaccat cacactcga 
        caacacacc catcgacct tgtgccatc cacaaggta 
        cctaggaat cacctacac acacacatg ctcactcga 
        caccgacct ccagaacac gctcaagaa agtccgcaa 
        ccgatccaa cctcctccg ccatctcat gggaaactt 
        ccaagggtg ccattcccg cactcttct ccacaccta 
        caactcatt catccgacc cgtcatcga gtaccgagc 
        ccccatcta cgcatctct cccacacac caagacaca 
        tcgcattgc cacatgcga acgacaaat ccttcgccg 
        aattcaccg cctcgaccg cttccacgc cagcaattc 
        cctccacca ggacacaaa caccacccc catcacaga 
        ccgtcttca caaactcca acaaaacta catcaaacg 
        cacactcaa caacaacaa cacaattgc ccaaaacac 
        actcaacac ttcacataa gtttctcac acgcgacaa 
        cactcttcg caaccgagt cccccggat ccccagaag 
        aaaactgcc ccatccacc cacggcgct tctctcagc 
        gctgtaccc cgacctgcc cgacaatct tcaacttct 
        cgttgaaga gacgcccct cacaatgag acgccactg 
        acgacaccg acaagacac ccacgacga catccacat 
        ccacagctt cctacgagc cccaaactt ctcactctt 
        tcactcacc acccgacga cacacacac tcccacaca 
        acattcatc catttcctt gactgaaga ggccaaaag 
        cctaaaaca gtccggact cgtagatta agttgtaaa 
        tatcttgta tatatttta tcaaaatcc cttttgaaa 
        tgtaattta ttttgaata aacgtattt tgaattgaa 
        ttgaatttg aattagtgc accatgatt ttagcttga 
        actttcaag ttgacaggt atattttgt gttcgttgt 
        gcgtattac agttcgttt gtgtcgtgt ttttttttt 
        ctttattaa gattagaaa tattaaaaa atatgccac 
        caaagacaa gcggtaaag cagcgaaaa aggctggaa 
        aagctcaaa agaatattt cgaagagcg acaagaaaa 
        agaagcgca ggaggaagg aaagctatg caatctata 
        tttataagg tattgaagc aagtacacc ccgataccg 
        gtatttcga gcaaggcga tgagcatca tgaacagtt 
        tcgttaatg atatctttg aacggatcg ccgctgaag 
        cttctcgtc ttgcgcatt acaacaagc gttcgacga 
        ttacgagcc gggaaattc aaacagcgg ttcgtctct 
        tgttgcctg gtgaattgg caaagcacg cagtctctg 
        aaggtacca aagccgtca ccaaataca caagtatat 
        ataaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY