Status
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Gene information
General information
TC006924 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450492.1: 3912..9223
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006924
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04238

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006924 RA
      MRISLNLSK FFEDHRSLA KIFINKNFK CIKDVEKHI 
      TNIFGIENF YLKCDNHYL PEAEDVHVL NEDEVVWAI 
      PFDETTVDF TFDATEEEE KPKVKKKKK HKSDETMEV 
      EEEPLIQKK KKTKKKTEK LDEPETKNM KEERRENEA 
      EFERKKKKR SFDPTDTST IERKKPKED FVSQNNGNL 
      KNSSSSGDE SFTPLGQNS TSVKSLVDS FENKCARKN 
      HPFINNAFE KRVNIVKTI IISNGKTDM LKDKKLEDS 
      SGVHSNSSA DNINTENND ASIRDDSSI TTSNTDPSN 
      LDICQPRKR KRTRRHKNK KKTIDYDFL RNLNVSDFL 
      ETSKASTIE FKPLEKAPV HVRFDEEDS DKSQVKNIT 
      DYEFLNTSK TSNCIIKFK KPLEQAPVS EEVSNETKV 
      ENKTENEKQ EKSEENKCN GDVVTKPAP EKNEIANGK 
      LNGDQVITL DEPFDEETF VKCMLQFPV ISGIEPRIG 
      DVIAFKILR ISENYTPEI SSWIVGKVV NYKNKEVTF 
      DITHGQDQC VNPQGKFCL EQEEPEEQS NEFKCLWQN 
      VIEPRCIYP
    CDS sequence
    • >TC006924 RA
      atgaggatc agtctgaat ttgagcaag ttttttgaa 
      gatcaccgc tctttggcc aaaattttc ataaacaaa 
      aatttcaaa tgtataaaa gacgttgaa aaacacata 
      acgaacatt ttcggaatt gagaatttc tatttaaag 
      tgtgacaac cactatttg ccagaagct gaagacgtt 
      catgtgcta aatgaggat gaagtggtg tgggccatt 
      ccgttcgat gagaccaca gttgatttc acatttgat 
      gctactgaa gaggaagaa aaaccaaag gtaaaaaag 
      aagaagaaa cacaagagt gatgaaaca atggaagta 
      gaagaggag ccgctgatc cagaaaaag aagaaaacg 
      aagaaaaaa actgaaaag ttggatgag cctgaaacg 
      aaaaatatg aaagaagaa agaagagaa aatgaagcc 
      gagtttgaa agaaagaag aaaaaaaga tcttttgat 
      cctacggat acttcaact attgagagg aaaaagccc 
      aaagaagac tttgtttcc caaaataac ggaaacttg 
      aaaaactca tctagttcc ggtgatgaa agttttaca 
      ccacttgga caaaacagc acgagcgtt aagtcactc 
      gttgacagt tttgaaaat aagtgtgct aggaaaaac 
      caccccttt ataaataat gccttcgaa aaacgagta 
      aatattgtg aaaactata ataatttca aatggaaag 
      actgacatg ctgaaagat aagaagtta gaagactcg 
      tcaggagtg cacagtaac agctcagct gataatata 
      aatacagaa aataacgat gcaagtatt agagatgat 
      agcagtatt actacaagt aatacagat ccttcaaac 
      cttgatatt tgtcagcct aggaagagg aaacgcact 
      cgccgtcac aaaaacaag aaaaaaact attgattac 
      gattttcta aggaattta aatgtttcc gacttttta 
      gaaacttct aaggcttct acgatcgag ttcaaacca 
      ttagaaaaa gctcctgtt cacgttaga tttgacgaa 
      gaagattca gacaaaagt caagttaaa aatattact 
      gattacgaa tttttaaac acttctaag accagtaat 
      tgtataatc aagtttaaa aaaccatta gaacaagct 
      ccggtttca gaagaagtt tcaaacgaa actaaagtt 
      gaaaacaag actgaaaac gaaaaacaa gaaaaatct 
      gaggaaaac aaatgtaac ggtgacgtg gttacaaaa 
      ccggctcct gaaaaaaat gaaatagct aatggaaag 
      ttgaatggt gatcaagtt ataactcta gatgagcct 
      tttgatgaa gaaacattt gttaagtgt atgttgcaa 
      tttcctgtc atttcggga attgaaccc agaattggg 
      gatgtgata gcgttcaag attcttagg atttctgaa 
      aattacacg cctgagata tcgtcctgg atagtggga 
      aaagttgta aactataaa aataaggag gttacgttt 
      gatattaca catgggcaa gatcagtgt gtgaatcct 
      caaggcaag ttttgtcta gagcaggaa gaaccagaa 
      gaacaaagt aacgaattc aaatgtttg tggcagaat 
      gtaatagaa ccgaggtgt atttatccc taa
    mRNA sequence
    • >TC006924 RA
      ttgtactaa gctatattc tatattaga attgagcaa 
      gaattgtat taattctaa tacaatcat ttacgttca 
      acgctaagt aagtcctga ccaagactc tgcccccgc 
      tggcagctg atgacctct gttatattt gtggactta 
      ttgttgatt gtttttatg tttatatag tctattttt 
      tggttgaag ttactttag tcgtactat taagcaaaa 
      tagtataaa aaagtagta tgctcacaa aacaggata 
      ggaggacgt agtggtgct tacctttga atgtgctac 
      aattatttt cggttgaat tttttgata ttgaggttg 
      atattatca aggttgtaa cgtgataac gaattgtac 
      aggatgtta ccgtaatat taagataac acaattgta 
      aaaatgact gcagttgaa cgttttact tgttcttca 
      ttaccatta caactgcct tgatggcat gccttaacg 
      atgaaattt ttaactttt atatgtatt tgttggaaa 
      aatgcttcc actgcagtg gacttttta tacacacgt 
      actgaaacg aggaaatta ttttgagta aagaaattt 
      atacgtatt tacacaatg ttactgttg aatgtaaac 
      tacattact ctatttata cgatgattt ttacaatgc 
      aagtgttaa tattataaa cggcctcaa caatacttt 
      aaaatgtga atttattga tacgcagct tatctccgc 
      aagagtaca acgtataat gtaatttac tagaagatt 
      aaaagtgtt caaaaatag agatattta aatataaca 
      actgtaatt gctgttgtg agcattaaa ttatgtaaa 
      attatgcaa ccctttcat tccataatg tagtctccg 
      aaaggctgt ttcagacaa tcttttttg tccgaaaga 
      ggaaaaaat catgtataa gacacaggt tgtctaaaa 
      cagcctcca tcaatcaat caattaaac atattgagt 
      acttattgt tttaattca atgtgaaac aagtgcccc 
      aacttgatt tttcgtgtg acctcggtc aaggtcata 
      aataactaa aaaaaataa attttggca ggtaatgat 
      atttttaac tgttaaggt aattcaaaa ctgttatag 
      aaagtgctt tatttggta tcaagctca attttgtaa 
      ccaacataa cctcaaatt ttcgtatgt gttttcggc 
      aaagttttc gtttgtcct ctgtgtttt aaagtgtta 
      ttagcccag aagagggca agtgtttgc aacaaaatg 
      aggatcagt ctgaatttg agcaagttt tttgaagat 
      caccgctct ttggccaaa attttcata aacaaaaat 
      ttcaaatgt ataaaagac gttgaaaaa cacataacg 
      aacattttc ggaattgag aatttctat ttaaagtgt 
      gacaaccac tatttgcca gaagctgaa gacgttcat 
      gtgctaaat gaggatgaa gtggtgtgg gccattccg 
      ttcgatgag accacagtt gatttcaca tttgatgct 
      actgaagag gaagaaaaa ccaaaggta aaaaagaag 
      aagaaacac aagagtgat gaaacaatg gaagtagaa 
      gaggagccg ctgatccag aaaaagaag aaaacgaag 
      aaaaaaact gaaaagttg gatgagcct gaaacgaaa 
      aatatgaaa gaagaaaga agagaaaat gaagccgag 
      tttgaaaga aagaagaaa aaaagatct tttgatcct 
      acggatact tcaactatt gagaggaaa aagcccaaa 
      gaagacttt gtttcccaa aataacgga aacttgaaa 
      aactcatct agttccggt gatgaaagt tttacacca 
      cttggacaa aacagcacg agcgttaag tcactcgtt 
      gacagtttt gaaaataag tgtgctagg aaaaaccac 
      ccctttata aataatgcc ttcgaaaaa cgagtaaat 
      attgtgaaa actataata atttcaaat ggaaagact 
      gacatgctg aaagataag aagttagaa gactcgtca 
      ggagtgcac agtaacagc tcagctgat aatataaat 
      acagaaaat aacgatgca agtattaga gatgatagc 
      agtattact acaagtaat acagatcct tcaaacctt 
      gatatttgt cagcctagg aagaggaaa cgcactcgc 
      cgtcacaaa aacaagaaa aaaactatt gattacgat 
      tttctaagg aatttaaat gtttccgac tttttagaa 
      acttctaag gcttctacg atcgagttc aaaccatta 
      gaaaaagct cctgttcac gttagattt gacgaagaa 
      gattcagac aaaagtcaa gttaaaaat attactgat 
      tacgaattt ttaaacact tctaagacc agtaattgt 
      ataatcaag tttaaaaaa ccattagaa caagctccg 
      gtttcagaa gaagtttca aacgaaact aaagttgaa 
      aacaagact gaaaacgaa aaacaagaa aaatctgag 
      gaaaacaaa tgtaacggt gacgtggtt acaaaaccg 
      gctcctgaa aaaaatgaa atagctaat ggaaagttg 
      aatggtgat caagttata actctagat gagcctttt 
      gatgaagaa acatttgtt aagtgtatg ttgcaattt 
      cctgtcatt tcgggaatt gaacccaga attggggat 
      gtgatagcg ttcaagatt cttaggatt tctgaaaat 
      tacacgcct gagatatcg tcctggata gtgggaaaa 
      gttgtaaac tataaaaat aaggaggtt acgtttgat 
      attacacat gggcaagat cagtgtgtg aatcctcaa 
      ggcaagttt tgtctagag caggaagaa ccagaagaa 
      caaagtaac gaattcaaa tgtttgtgg cagaatgta 
      atagaaccg aggtgtatt tatccctaa cacgttaat 
      gtttcttct tattattcc tgttaagtg tagtgatga 
      aactactgt tataaaata ttttgtttg taaaattct 
      acaataaat ttatgaaat cgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY