| General information | |||
| TC006914 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451240.1: 993..4976 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006914 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04237 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC006914 RA
MPPLRNSDR VLVSEANSE IFSFDDDLF AKPRKKRVR KIKCDICGK YIKSTRMQL HKEKENKKN LKTFQFDDD IFAKPKKNR VKKVKCDMC GTYIEETRI HLHKLKHDL NSRRLLELR NMAKSLGIR YIKRYNKAE LINEIINAE EQLKIKREN KKSLEIFPF DDAIFSKLK KERVRKIKC DECGKYIKE SRMQLHKAR HNLEPDFIL VQVAFKGRL LTYQHNNLE EGIDMETYM DMIKEKAIK IIINALNLH KNIKTNLEM ECEFMKLEN EDYTMIHKF QLPNVILRE KDALEEYWD EQKDLFISR CDELQEKGS GWTLKNINH LRININKYT PLSGNSYVE LPKWIKDKK ACVNVKNND NKCFMYSII SCLHQAKEH VDRLNHYNK PEYINELNF EGINFPVKI QDIPKFESH NKINVTVFS YEEQIFYPL YHNKKRFYE MNIDLLYFG NKDNNHYCW IKNLSRLLN DQLEINRSK KYFCRRCLN YSARTEDAL KKHQNVCFS NEPCVPNMP DDNVLKFKN IQRMLRHPY VIYSDFESI LKPIPTCEP NPESSYTHK TQFHVPCGY GLYIKSAYN NIDNPLEIY RGEESDKRF IKRITEHTR TIYKLLKEN KPIKDIELE EYEKTKICY LCENVFSIE NPKVRDHDH YTGKYRGAA CRKCNLKYR QPLFIPILF HNLSSYDCH LFIKNLGED KSQLKLIPN NEEKYISFG KILRMDDNG YIELRFIDS FRFLASSLD NLSSNLESS QLREIIKYF SNSELDVLF RKEKSKIIR KGIYPYEYM DSFEKFFET KLPSIDKFY SSLTKEGIS QEEFEHAVK VWKHFKIKN LGEWHDLYL KLDVLLLTD VFENFRDLC LEKSYGLDP VWYYTTPGL AWDCMLKYT SVELELLTD YEMYLFFEK GIRGGISQC SNRYSQADE NTQIIYIDA NNLYGWALS QKLPEKDFK WLTCDEIKN VDGILVSDS DTGYVLEVD LAFPVDLAI HNKFNDLPL APENRIPPG GKVEKLLCH FYDKKNYII HYKTLKLYK SLGIRITKV HRGIKFTQS NFIAPYVKF NTEQRQKAK NDFEKDFWK LMVNAPYGK SMQSVRNQQ NIKLYCDEE KLQKAINKP NFKHRTIFS KHLAAVHNF KTKVLFNKP IYIGQAVLD LSKLLMYNF HYNVIKKEF NQNVKLLYG DTDSFIYEI KNADFYEFM KNNPDYFDT SDYPKDHSL YSDKNKKVI GKFKDELNG IKILECIGL RSKMYSYRT ETSVSKKLK GIKKAVLKK EISFEDYKN CLFNQKEYF HTQKVIRSH KHDIFTEEV KKKSLSWKD DKRYLLPDS TDTLAYGHV LVSS
- >TC006914 RA
atgcctcct ttgcggaat tcggacaga gtcctcgtg agcgaagcg aattcagaa atattttca tttgatgat gatttattt gctaaacca aggaaaaag agagttagg aaaatcaaa tgtgacata tgtggaaaa tatatcaaa agcacccga atgcagctc cataaagag aaagaaaac aaaaagaat ctaaaaaca tttcagttt gatgacgat atatttgct aaaccaaaa aaaaataga gtaaaaaag gtaaaatgt gatatgtgt ggaacatat atcgaggaa actcgaata catttgcat aaattaaaa catgatttg aatagtcgt cgtttgctc gaattacgt aatatggca aaatcctta ggtataaga tacattaaa agatacaat aaggcagaa ttgataaac gagattatt aatgctgaa gagcaatta aaaataaaa agagaaaac aagaagagc ttggaaata tttccgttt gatgatgcc atattttct aaactaaaa aaagaaaga gtaagaaag atcaaatgt gatgaatgt ggaaagtat attaaggaa agtcgaatg cagttgcat aaagcaaga cataatcta gagccagat tttatttta gttcaggtt gcttttaag ggaagatta ttaacatat caacataat aatctagaa gaaggtata gatatggaa acatacatg gatatgata aaagaaaag gctattaaa ataattata aatgcttta aatctccat aaaaatatt aaaaccaat ctagagatg gaatgtgag tttatgaag ctggaaaat gaagactac acaatgata cataaattt cagttacca aatgttata ctaagagaa aaagacgcc ttagaggaa tactgggat gaacaaaag gatttattt atatcaaga tgtgatgaa ctacaagaa aaaggatct ggatggact ctgaaaaat ataaatcat ttgcgcatt aatataaac aaatataca cctttaagc ggaaattca tatgtagaa ttacccaag tggattaaa gataaaaaa gcttgcgtt aatgttaaa aacaacgat aacaaatgc tttatgtac agcataata tcctgttta catcaagca aaagaacat gtagataga ctgaatcat tataataag ccggaatat ataaacgaa ttaaatttt gaaggaata aattttcct gtaaaaatc caggacata cctaaattt gaatcacat aataaaatt aatgttacc gtgtttagt tacgaagaa caaatattt tatcctttg tatcataat aagaagcga ttttatgaa atgaatatc gacttacta tactttgga aataaagac aacaatcat tattgttgg atcaagaat ttgagtcga ttgttaaat gatcagttg gaaataaat agaagcaaa aaatatttc tgtagaaga tgtttaaac tactccgct agaactgaa gatgcttta aagaaacat caaaatgtg tgctttagt aatgaacca tgtgttccg aacatgcct gatgataat gtgttaaaa tttaaaaat attcaaaga atgttaaga catccgtat gtcatttat tctgatttt gaatctatt ctgaaacct attcctaca tgcgaacca aatcctgaa tcatcatat actcataaa acacaattt catgttccc tgtggttat ggattatac attaagagt gcttataat aatattgat aacccattg gaaatttac agaggagag gaaagtgat aaacgtttt ataaaaaga ataactgaa cataccaga acaatatat aagttatta aaagaaaat aaacccata aaagatatc gaactagaa gaatatgaa aaaacaaaa atttgttat ctttgtgaa aatgtgttt tctatagaa aatccaaaa gtaagagat catgatcac tacactgga aaatatagg ggagctgct tgtagaaaa tgcaatttg aaatataga caaccattg tttattcca attttgttt cataattta agttcttac gattgtcat ttgtttata aaaaatcta ggagaagat aaaagtcaa ttgaaatta atacctaat aatgaagag aagtatatt tctttcggt aaaatttta agaatggat gataatgga tatatcgaa ttgagattt atcgattca tttagattt ttagcaagt agcctagac aatttgtca tctaatctg gaatcaagt cagttgaga gaaataatt aaatacttt tcaaatagt gaattagat gtgttattt aggaaagaa aaaagcaag ataattaga aaaggaata tatccttat gaatacatg gattctttt gagaaattt tttgaaaca aaacttcca tcaatagat aaattttat tcttcgctc acaaaagaa ggaatttct caagaagaa ttcgaacat gcagtgaaa gtatggaaa cattttaaa atcaaaaat ttgggtgaa tggcatgat ttatactta aaactagat gttttgctg ttaactgat gtatttgaa aattttaga gacttatgt ttggaaaag agttatgga ctcgatcct gtttggtat tatacaacg ccggggtta gcttgggac tgtatgctc aaatatact tcagtggag ttggaatta ttaactgat tatgaaatg tatttgttt tttgaaaag ggaattaga ggaggtatt tcacagtgt agtaacagg tattcgcaa gcagatgaa aatactcag attatctat atagatgct aataatctc tatggatgg gctttatct cagaaatta ccagaaaaa gactttaaa tggttgact tgtgatgaa attaaaaat gtcgacggt atattggta tcagatagt gatacgggt tatgttttg gaagtcgat ttagcattt ccagtagac ttagccatt cataacaaa ttcaatgat cttccttta gctccggaa aatagaatt cctccgggc ggaaaagta gaaaaactt ctttgtcat ttctatgat aagaaaaat tatataatt cattacaaa acattaaaa ctttacaaa agtttaggt attagaata acaaaggtt cacagaggt ataaaattt actcagtca aattttata gcaccatat gtaaaattt aatactgaa caaagacaa aaggcgaaa aatgatttt gaaaaagat ttctggaaa ttaatggtt aatgctcca tatggtaaa tctatgcaa tctgttaga aaccaacaa aatattaaa ttatattgt gatgaagaa aaactacaa aaagcgata aacaaacct aattttaaa cacagaact atatttagc aaacacctg gcagcagtt cacaatttt aaaacaaaa gtattgttt aataaacca atttatatt ggacaagct gtgttagat ttatcgaaa ttattaatg tataacttc cattataat gtaattaaa aaagaattc aatcaaaac gtaaaactt ctttatgga gacactgat agctttata tatgagatt aaaaatgcc gatttctat gaatttatg aaaaacaat cccgactat tttgataca tctgattat ccaaaggat cattcatta tattctgat aaaaataag aaagtaata gggaagttc aaagatgaa ttgaatggt attaagata ttagaatgc ataggttta aggtcaaaa atgtacagt tacagaact gaaacgtct gtttcgaaa aaattaaaa ggtatcaag aaagcagta ttgaagaaa gaaataagt tttgaagat tacaaaaat tgcttattt aatcaaaaa gaatacttc catacacag aaagtgatc aggagtcat aaacatgat atatttact gaagaagta aagaaaaag tctctaagt tggaaagat gataaaaga tacctttta cctgattct acagatacg ttagcttat ggacacgta ttagtatca tcatag
- >TC006914 RA
atgcctcct ttgcggaat tcggacaga gtcctcgtg agcgaagcg aattcagaa atattttca tttgatgat gatttattt gctaaacca aggaaaaag agagttagg aaaatcaaa tgtgacata tgtggaaaa tatatcaaa agcacccga atgcagctc cataaagag aaagaaaac aaaaagaat ctaaaaaca tttcagttt gatgacgat atatttgct aaaccaaaa aaaaataga gtaaaaaag gtaaaatgt gatatgtgt ggaacatat atcgaggaa actcgaata catttgcat aaattaaaa catgatttg aatagtcgt cgtttgctc gaattacgt aatatggca aaatcctta ggtataaga tacattaaa agatacaat aaggcagaa ttgataaac gagattatt aatgctgaa gagcaatta aaaataaaa agagaaaac aagaagagc ttggaaata tttccgttt gatgatgcc atattttct aaactaaaa aaagaaaga gtaagaaag atcaaatgt gatgaatgt ggaaagtat attaaggaa agtcgaatg cagttgcat aaagcaaga cataatcta gagccagat tttatttta gttcaggtt gcttttaag ggaagatta ttaacatat caacataat aatctagaa gaaggtata gatatggaa acatacatg gatatgata aaagaaaag gctattaaa ataattata aatgcttta aatctccat aaaaatatt aaaaccaat ctagagatg gaatgtgag tttatgaag ctggaaaat gaagactac acaatgata cataaattt cagttacca aatgttata ctaagagaa aaagacgcc ttagaggaa tactgggat gaacaaaag gatttattt atatcaaga tgtgatgaa ctacaagaa aaaggatct ggatggact ctgaaaaat ataaatcat ttgcgcatt aatataaac aaatataca cctttaagc ggaaattca tatgtagaa ttacccaag tggattaaa gataaaaaa gcttgcgtt aatgttaaa aacaacgat aacaaatgc tttatgtac agcataata tcctgttta catcaagca aaagaacat gtagataga ctgaatcat tataataag ccggaatat ataaacgaa ttaaatttt gaaggaata aattttcct gtaaaaatc caggacata cctaaattt gaatcacat aataaaatt aatgttacc gtgtttagt tacgaagaa caaatattt tatcctttg tatcataat aagaagcga ttttatgaa atgaatatc gacttacta tactttgga aataaagac aacaatcat tattgttgg atcaagaat ttgagtcga ttgttaaat gatcagttg gaaataaat agaagcaaa aaatatttc tgtagaaga tgtttaaac tactccgct agaactgaa gatgcttta aagaaacat caaaatgtg tgctttagt aatgaacca tgtgttccg aacatgcct gatgataat gtgttaaaa tttaaaaat attcaaaga atgttaaga catccgtat gtcatttat tctgatttt gaatctatt ctgaaacct attcctaca tgcgaacca aatcctgaa tcatcatat actcataaa acacaattt catgttccc tgtggttat ggattatac attaagagt gcttataat aatattgat aacccattg gaaatttac agaggagag gaaagtgat aaacgtttt ataaaaaga ataactgaa cataccaga acaatatat aagttatta aaagaaaat aaacccata aaagatatc gaactagaa gaatatgaa aaaacaaaa atttgttat ctttgtgaa aatgtgttt tctatagaa aatccaaaa gtaagagat catgatcac tacactgga aaatatagg ggagctgct tgtagaaaa tgcaatttg aaatataga caaccattg tttattcca attttgttt cataattta agttcttac gattgtcat ttgtttata aaaaatcta ggagaagat aaaagtcaa ttgaaatta atacctaat aatgaagag aagtatatt tctttcggt aaaatttta agaatggat gataatgga tatatcgaa ttgagattt atcgattca tttagattt ttagcaagt agcctagac aatttgtca tctaatctg gaatcaagt cagttgaga gaaataatt aaatacttt tcaaatagt gaattagat gtgttattt aggaaagaa aaaagcaag ataattaga aaaggaata tatccttat gaatacatg gattctttt gagaaattt tttgaaaca aaacttcca tcaatagat aaattttat tcttcgctc acaaaagaa ggaatttct caagaagaa ttcgaacat gcagtgaaa gtatggaaa cattttaaa atcaaaaat ttgggtgaa tggcatgat ttatactta aaactagat gttttgctg ttaactgat gtatttgaa aattttaga gacttatgt ttggaaaag agttatgga ctcgatcct gtttggtat tatacaacg ccggggtta gcttgggac tgtatgctc aaatatact tcagtggag ttggaatta ttaactgat tatgaaatg tatttgttt tttgaaaag ggaattaga ggaggtatt tcacagtgt agtaacagg tattcgcaa gcagatgaa aatactcag attatctat atagatgct aataatctc tatggatgg gctttatct cagaaatta ccagaaaaa gactttaaa tggttgact tgtgatgaa attaaaaat gtcgacggt atattggta tcagatagt gatacgggt tatgttttg gaagtcgat ttagcattt ccagtagac ttagccatt cataacaaa ttcaatgat cttccttta gctccggaa aatagaatt cctccgggc ggaaaagta gaaaaactt ctttgtcat ttctatgat aagaaaaat tatataatt cattacaaa acattaaaa ctttacaaa agtttaggt attagaata acaaaggtt cacagaggt ataaaattt actcagtca aattttata gcaccatat gtaaaattt aatactgaa caaagacaa aaggcgaaa aatgatttt gaaaaagat ttctggaaa ttaatggtt aatgctcca tatggtaaa tctatgcaa tctgttaga aaccaacaa aatattaaa ttatattgt gatgaagaa aaactacaa aaagcgata aacaaacct aattttaaa cacagaact atatttagc aaacacctg gcagcagtt cacaatttt aaaacaaaa gtattgttt aataaacca atttatatt ggacaagct gtgttagat ttatcgaaa ttattaatg tataacttc cattataat gtaattaaa aaagaattc aatcaaaac gtaaaactt ctttatgga gacactgat agctttata tatgagatt aaaaatgcc gatttctat gaatttatg aaaaacaat cccgactat tttgataca tctgattat ccaaaggat cattcatta tattctgat aaaaataag aaagtaata gggaagttc aaagatgaa ttgaatggt attaagata ttagaatgc ataggttta aggtcaaaa atgtacagt tacagaact gaaacgtct gtttcgaaa aaattaaaa ggtatcaag aaagcagta ttgaagaaa gaaataagt tttgaagat tacaaaaat tgcttattt aatcaaaaa gaatacttc catacacag aaagtgatc aggagtcat aaacatgat atatttact gaagaagta aagaaaaag tctctaagt tggaaagat gataaaaga tacctttta cctgattct acagatacg ttagcttat ggacacgta ttagtatca tcatag
