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Gene information
General information
TC006914 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451240.1: 993..4976
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006914
      iBeetle screen iB_04237

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006914 RA
        MPPLRNSDR VLVSEANSE IFSFDDDLF AKPRKKRVR 
        KIKCDICGK YIKSTRMQL HKEKENKKN LKTFQFDDD 
        IFAKPKKNR VKKVKCDMC GTYIEETRI HLHKLKHDL 
        NSRRLLELR NMAKSLGIR YIKRYNKAE LINEIINAE 
        EQLKIKREN KKSLEIFPF DDAIFSKLK KERVRKIKC 
        DECGKYIKE SRMQLHKAR HNLEPDFIL VQVAFKGRL 
        LTYQHNNLE EGIDMETYM DMIKEKAIK IIINALNLH 
        KNIKTNLEM ECEFMKLEN EDYTMIHKF QLPNVILRE 
        KDALEEYWD EQKDLFISR CDELQEKGS GWTLKNINH 
        LRININKYT PLSGNSYVE LPKWIKDKK ACVNVKNND 
        NKCFMYSII SCLHQAKEH VDRLNHYNK PEYINELNF 
        EGINFPVKI QDIPKFESH NKINVTVFS YEEQIFYPL 
        YHNKKRFYE MNIDLLYFG NKDNNHYCW IKNLSRLLN 
        DQLEINRSK KYFCRRCLN YSARTEDAL KKHQNVCFS 
        NEPCVPNMP DDNVLKFKN IQRMLRHPY VIYSDFESI 
        LKPIPTCEP NPESSYTHK TQFHVPCGY GLYIKSAYN 
        NIDNPLEIY RGEESDKRF IKRITEHTR TIYKLLKEN 
        KPIKDIELE EYEKTKICY LCENVFSIE NPKVRDHDH 
        YTGKYRGAA CRKCNLKYR QPLFIPILF HNLSSYDCH 
        LFIKNLGED KSQLKLIPN NEEKYISFG KILRMDDNG 
        YIELRFIDS FRFLASSLD NLSSNLESS QLREIIKYF 
        SNSELDVLF RKEKSKIIR KGIYPYEYM DSFEKFFET 
        KLPSIDKFY SSLTKEGIS QEEFEHAVK VWKHFKIKN 
        LGEWHDLYL KLDVLLLTD VFENFRDLC LEKSYGLDP 
        VWYYTTPGL AWDCMLKYT SVELELLTD YEMYLFFEK 
        GIRGGISQC SNRYSQADE NTQIIYIDA NNLYGWALS 
        QKLPEKDFK WLTCDEIKN VDGILVSDS DTGYVLEVD 
        LAFPVDLAI HNKFNDLPL APENRIPPG GKVEKLLCH 
        FYDKKNYII HYKTLKLYK SLGIRITKV HRGIKFTQS 
        NFIAPYVKF NTEQRQKAK NDFEKDFWK LMVNAPYGK 
        SMQSVRNQQ NIKLYCDEE KLQKAINKP NFKHRTIFS 
        KHLAAVHNF KTKVLFNKP IYIGQAVLD LSKLLMYNF 
        HYNVIKKEF NQNVKLLYG DTDSFIYEI KNADFYEFM 
        KNNPDYFDT SDYPKDHSL YSDKNKKVI GKFKDELNG 
        IKILECIGL RSKMYSYRT ETSVSKKLK GIKKAVLKK 
        EISFEDYKN CLFNQKEYF HTQKVIRSH KHDIFTEEV 
        KKKSLSWKD DKRYLLPDS TDTLAYGHV LVSS
      CDS sequence
      • >TC006914 RA
        atgcctcct ttgcggaat tcggacaga gtcctcgtg 
        agcgaagcg aattcagaa atattttca tttgatgat 
        gatttattt gctaaacca aggaaaaag agagttagg 
        aaaatcaaa tgtgacata tgtggaaaa tatatcaaa 
        agcacccga atgcagctc cataaagag aaagaaaac 
        aaaaagaat ctaaaaaca tttcagttt gatgacgat 
        atatttgct aaaccaaaa aaaaataga gtaaaaaag 
        gtaaaatgt gatatgtgt ggaacatat atcgaggaa 
        actcgaata catttgcat aaattaaaa catgatttg 
        aatagtcgt cgtttgctc gaattacgt aatatggca 
        aaatcctta ggtataaga tacattaaa agatacaat 
        aaggcagaa ttgataaac gagattatt aatgctgaa 
        gagcaatta aaaataaaa agagaaaac aagaagagc 
        ttggaaata tttccgttt gatgatgcc atattttct 
        aaactaaaa aaagaaaga gtaagaaag atcaaatgt 
        gatgaatgt ggaaagtat attaaggaa agtcgaatg 
        cagttgcat aaagcaaga cataatcta gagccagat 
        tttatttta gttcaggtt gcttttaag ggaagatta 
        ttaacatat caacataat aatctagaa gaaggtata 
        gatatggaa acatacatg gatatgata aaagaaaag 
        gctattaaa ataattata aatgcttta aatctccat 
        aaaaatatt aaaaccaat ctagagatg gaatgtgag 
        tttatgaag ctggaaaat gaagactac acaatgata 
        cataaattt cagttacca aatgttata ctaagagaa 
        aaagacgcc ttagaggaa tactgggat gaacaaaag 
        gatttattt atatcaaga tgtgatgaa ctacaagaa 
        aaaggatct ggatggact ctgaaaaat ataaatcat 
        ttgcgcatt aatataaac aaatataca cctttaagc 
        ggaaattca tatgtagaa ttacccaag tggattaaa 
        gataaaaaa gcttgcgtt aatgttaaa aacaacgat 
        aacaaatgc tttatgtac agcataata tcctgttta 
        catcaagca aaagaacat gtagataga ctgaatcat 
        tataataag ccggaatat ataaacgaa ttaaatttt 
        gaaggaata aattttcct gtaaaaatc caggacata 
        cctaaattt gaatcacat aataaaatt aatgttacc 
        gtgtttagt tacgaagaa caaatattt tatcctttg 
        tatcataat aagaagcga ttttatgaa atgaatatc 
        gacttacta tactttgga aataaagac aacaatcat 
        tattgttgg atcaagaat ttgagtcga ttgttaaat 
        gatcagttg gaaataaat agaagcaaa aaatatttc 
        tgtagaaga tgtttaaac tactccgct agaactgaa 
        gatgcttta aagaaacat caaaatgtg tgctttagt 
        aatgaacca tgtgttccg aacatgcct gatgataat 
        gtgttaaaa tttaaaaat attcaaaga atgttaaga 
        catccgtat gtcatttat tctgatttt gaatctatt 
        ctgaaacct attcctaca tgcgaacca aatcctgaa 
        tcatcatat actcataaa acacaattt catgttccc 
        tgtggttat ggattatac attaagagt gcttataat 
        aatattgat aacccattg gaaatttac agaggagag 
        gaaagtgat aaacgtttt ataaaaaga ataactgaa 
        cataccaga acaatatat aagttatta aaagaaaat 
        aaacccata aaagatatc gaactagaa gaatatgaa 
        aaaacaaaa atttgttat ctttgtgaa aatgtgttt 
        tctatagaa aatccaaaa gtaagagat catgatcac 
        tacactgga aaatatagg ggagctgct tgtagaaaa 
        tgcaatttg aaatataga caaccattg tttattcca 
        attttgttt cataattta agttcttac gattgtcat 
        ttgtttata aaaaatcta ggagaagat aaaagtcaa 
        ttgaaatta atacctaat aatgaagag aagtatatt 
        tctttcggt aaaatttta agaatggat gataatgga 
        tatatcgaa ttgagattt atcgattca tttagattt 
        ttagcaagt agcctagac aatttgtca tctaatctg 
        gaatcaagt cagttgaga gaaataatt aaatacttt 
        tcaaatagt gaattagat gtgttattt aggaaagaa 
        aaaagcaag ataattaga aaaggaata tatccttat 
        gaatacatg gattctttt gagaaattt tttgaaaca 
        aaacttcca tcaatagat aaattttat tcttcgctc 
        acaaaagaa ggaatttct caagaagaa ttcgaacat 
        gcagtgaaa gtatggaaa cattttaaa atcaaaaat 
        ttgggtgaa tggcatgat ttatactta aaactagat 
        gttttgctg ttaactgat gtatttgaa aattttaga 
        gacttatgt ttggaaaag agttatgga ctcgatcct 
        gtttggtat tatacaacg ccggggtta gcttgggac 
        tgtatgctc aaatatact tcagtggag ttggaatta 
        ttaactgat tatgaaatg tatttgttt tttgaaaag 
        ggaattaga ggaggtatt tcacagtgt agtaacagg 
        tattcgcaa gcagatgaa aatactcag attatctat 
        atagatgct aataatctc tatggatgg gctttatct 
        cagaaatta ccagaaaaa gactttaaa tggttgact 
        tgtgatgaa attaaaaat gtcgacggt atattggta 
        tcagatagt gatacgggt tatgttttg gaagtcgat 
        ttagcattt ccagtagac ttagccatt cataacaaa 
        ttcaatgat cttccttta gctccggaa aatagaatt 
        cctccgggc ggaaaagta gaaaaactt ctttgtcat 
        ttctatgat aagaaaaat tatataatt cattacaaa 
        acattaaaa ctttacaaa agtttaggt attagaata 
        acaaaggtt cacagaggt ataaaattt actcagtca 
        aattttata gcaccatat gtaaaattt aatactgaa 
        caaagacaa aaggcgaaa aatgatttt gaaaaagat 
        ttctggaaa ttaatggtt aatgctcca tatggtaaa 
        tctatgcaa tctgttaga aaccaacaa aatattaaa 
        ttatattgt gatgaagaa aaactacaa aaagcgata 
        aacaaacct aattttaaa cacagaact atatttagc 
        aaacacctg gcagcagtt cacaatttt aaaacaaaa 
        gtattgttt aataaacca atttatatt ggacaagct 
        gtgttagat ttatcgaaa ttattaatg tataacttc 
        cattataat gtaattaaa aaagaattc aatcaaaac 
        gtaaaactt ctttatgga gacactgat agctttata 
        tatgagatt aaaaatgcc gatttctat gaatttatg 
        aaaaacaat cccgactat tttgataca tctgattat 
        ccaaaggat cattcatta tattctgat aaaaataag 
        aaagtaata gggaagttc aaagatgaa ttgaatggt 
        attaagata ttagaatgc ataggttta aggtcaaaa 
        atgtacagt tacagaact gaaacgtct gtttcgaaa 
        aaattaaaa ggtatcaag aaagcagta ttgaagaaa 
        gaaataagt tttgaagat tacaaaaat tgcttattt 
        aatcaaaaa gaatacttc catacacag aaagtgatc 
        aggagtcat aaacatgat atatttact gaagaagta 
        aagaaaaag tctctaagt tggaaagat gataaaaga 
        tacctttta cctgattct acagatacg ttagcttat 
        ggacacgta ttagtatca tcatag
      mRNA sequence
      • >TC006914 RA
        atgcctcct ttgcggaat tcggacaga gtcctcgtg 
        agcgaagcg aattcagaa atattttca tttgatgat 
        gatttattt gctaaacca aggaaaaag agagttagg 
        aaaatcaaa tgtgacata tgtggaaaa tatatcaaa 
        agcacccga atgcagctc cataaagag aaagaaaac 
        aaaaagaat ctaaaaaca tttcagttt gatgacgat 
        atatttgct aaaccaaaa aaaaataga gtaaaaaag 
        gtaaaatgt gatatgtgt ggaacatat atcgaggaa 
        actcgaata catttgcat aaattaaaa catgatttg 
        aatagtcgt cgtttgctc gaattacgt aatatggca 
        aaatcctta ggtataaga tacattaaa agatacaat 
        aaggcagaa ttgataaac gagattatt aatgctgaa 
        gagcaatta aaaataaaa agagaaaac aagaagagc 
        ttggaaata tttccgttt gatgatgcc atattttct 
        aaactaaaa aaagaaaga gtaagaaag atcaaatgt 
        gatgaatgt ggaaagtat attaaggaa agtcgaatg 
        cagttgcat aaagcaaga cataatcta gagccagat 
        tttatttta gttcaggtt gcttttaag ggaagatta 
        ttaacatat caacataat aatctagaa gaaggtata 
        gatatggaa acatacatg gatatgata aaagaaaag 
        gctattaaa ataattata aatgcttta aatctccat 
        aaaaatatt aaaaccaat ctagagatg gaatgtgag 
        tttatgaag ctggaaaat gaagactac acaatgata 
        cataaattt cagttacca aatgttata ctaagagaa 
        aaagacgcc ttagaggaa tactgggat gaacaaaag 
        gatttattt atatcaaga tgtgatgaa ctacaagaa 
        aaaggatct ggatggact ctgaaaaat ataaatcat 
        ttgcgcatt aatataaac aaatataca cctttaagc 
        ggaaattca tatgtagaa ttacccaag tggattaaa 
        gataaaaaa gcttgcgtt aatgttaaa aacaacgat 
        aacaaatgc tttatgtac agcataata tcctgttta 
        catcaagca aaagaacat gtagataga ctgaatcat 
        tataataag ccggaatat ataaacgaa ttaaatttt 
        gaaggaata aattttcct gtaaaaatc caggacata 
        cctaaattt gaatcacat aataaaatt aatgttacc 
        gtgtttagt tacgaagaa caaatattt tatcctttg 
        tatcataat aagaagcga ttttatgaa atgaatatc 
        gacttacta tactttgga aataaagac aacaatcat 
        tattgttgg atcaagaat ttgagtcga ttgttaaat 
        gatcagttg gaaataaat agaagcaaa aaatatttc 
        tgtagaaga tgtttaaac tactccgct agaactgaa 
        gatgcttta aagaaacat caaaatgtg tgctttagt 
        aatgaacca tgtgttccg aacatgcct gatgataat 
        gtgttaaaa tttaaaaat attcaaaga atgttaaga 
        catccgtat gtcatttat tctgatttt gaatctatt 
        ctgaaacct attcctaca tgcgaacca aatcctgaa 
        tcatcatat actcataaa acacaattt catgttccc 
        tgtggttat ggattatac attaagagt gcttataat 
        aatattgat aacccattg gaaatttac agaggagag 
        gaaagtgat aaacgtttt ataaaaaga ataactgaa 
        cataccaga acaatatat aagttatta aaagaaaat 
        aaacccata aaagatatc gaactagaa gaatatgaa 
        aaaacaaaa atttgttat ctttgtgaa aatgtgttt 
        tctatagaa aatccaaaa gtaagagat catgatcac 
        tacactgga aaatatagg ggagctgct tgtagaaaa 
        tgcaatttg aaatataga caaccattg tttattcca 
        attttgttt cataattta agttcttac gattgtcat 
        ttgtttata aaaaatcta ggagaagat aaaagtcaa 
        ttgaaatta atacctaat aatgaagag aagtatatt 
        tctttcggt aaaatttta agaatggat gataatgga 
        tatatcgaa ttgagattt atcgattca tttagattt 
        ttagcaagt agcctagac aatttgtca tctaatctg 
        gaatcaagt cagttgaga gaaataatt aaatacttt 
        tcaaatagt gaattagat gtgttattt aggaaagaa 
        aaaagcaag ataattaga aaaggaata tatccttat 
        gaatacatg gattctttt gagaaattt tttgaaaca 
        aaacttcca tcaatagat aaattttat tcttcgctc 
        acaaaagaa ggaatttct caagaagaa ttcgaacat 
        gcagtgaaa gtatggaaa cattttaaa atcaaaaat 
        ttgggtgaa tggcatgat ttatactta aaactagat 
        gttttgctg ttaactgat gtatttgaa aattttaga 
        gacttatgt ttggaaaag agttatgga ctcgatcct 
        gtttggtat tatacaacg ccggggtta gcttgggac 
        tgtatgctc aaatatact tcagtggag ttggaatta 
        ttaactgat tatgaaatg tatttgttt tttgaaaag 
        ggaattaga ggaggtatt tcacagtgt agtaacagg 
        tattcgcaa gcagatgaa aatactcag attatctat 
        atagatgct aataatctc tatggatgg gctttatct 
        cagaaatta ccagaaaaa gactttaaa tggttgact 
        tgtgatgaa attaaaaat gtcgacggt atattggta 
        tcagatagt gatacgggt tatgttttg gaagtcgat 
        ttagcattt ccagtagac ttagccatt cataacaaa 
        ttcaatgat cttccttta gctccggaa aatagaatt 
        cctccgggc ggaaaagta gaaaaactt ctttgtcat 
        ttctatgat aagaaaaat tatataatt cattacaaa 
        acattaaaa ctttacaaa agtttaggt attagaata 
        acaaaggtt cacagaggt ataaaattt actcagtca 
        aattttata gcaccatat gtaaaattt aatactgaa 
        caaagacaa aaggcgaaa aatgatttt gaaaaagat 
        ttctggaaa ttaatggtt aatgctcca tatggtaaa 
        tctatgcaa tctgttaga aaccaacaa aatattaaa 
        ttatattgt gatgaagaa aaactacaa aaagcgata 
        aacaaacct aattttaaa cacagaact atatttagc 
        aaacacctg gcagcagtt cacaatttt aaaacaaaa 
        gtattgttt aataaacca atttatatt ggacaagct 
        gtgttagat ttatcgaaa ttattaatg tataacttc 
        cattataat gtaattaaa aaagaattc aatcaaaac 
        gtaaaactt ctttatgga gacactgat agctttata 
        tatgagatt aaaaatgcc gatttctat gaatttatg 
        aaaaacaat cccgactat tttgataca tctgattat 
        ccaaaggat cattcatta tattctgat aaaaataag 
        aaagtaata gggaagttc aaagatgaa ttgaatggt 
        attaagata ttagaatgc ataggttta aggtcaaaa 
        atgtacagt tacagaact gaaacgtct gtttcgaaa 
        aaattaaaa ggtatcaag aaagcagta ttgaagaaa 
        gaaataagt tttgaagat tacaaaaat tgcttattt 
        aatcaaaaa gaatacttc catacacag aaagtgatc 
        aggagtcat aaacatgat atatttact gaagaagta 
        aagaaaaag tctctaagt tggaaagat gataaaaga 
        tacctttta cctgattct acagatacg ttagcttat 
        ggacacgta ttagtatca tcatag
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY