Status
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Gene information
General information
TC000032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15867242..15880634
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000032
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04235

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000032 RA
      MIVQEPVQA AIWHCLNHY DYTDAVFLS ERLYAEVKS 
      DDSLYLLAT AYYRSGQKD HAYHILKER TDASTQCRY 
      LLGICAYDL EKYAEAEAA LLHSNKSSN DSENFDDIT 
      SEYGDQAPF ALSLLGNIA AKTERKPRA IDAWKRALK 
      LNPFQWSSF ENLCKIGDK PNPQNIFQI TGVENLSMC 
      QGSNINNIE SVVITNNNP NQDNQETYA TTPQQILVN 
      FSPNVVTSN SKLCTPDDN PIVTQNVWL SGFAPFTSR 
      TKLLRFRTD SSSNPSPQP SFGHFLDTS PDGYFSTPN 
      LLSQPKLTE SNNHHQSLA KRVKAQVDQ FINRKEPIL 
      QNCKPLFSQ STNVTKTPT ISLAVQPCQ NVRRSSRLF 
      TNSYSVKEN NKSPNRTKF ATPKSPSRK TKQRMAKCN 
      LNKTTAYTE LNTRNRIEK EKTETVTST DAKIEKSAI 
      SNSSSVESC VQQAILMQK QSAEGLMVL LRSLGQAYL 
      HLSNFNCKA AIEELNVLP PNQFQTAWI YCLLGLAYF 
      ELTDYESSI KYFSKVHNL EPYRIQFMD VYSTALWHL 
      QKEVALSAL AQDLISLNK NSPVTWCVS GNCFSLHKE 
      HDTAIKFFQ RAVQVDPRF PYAYTLLGH EYITTEELD 
      KAMSCFRNA IRLDPRHYN AWFGIGTIY SKQERYHLA 
      EINYSRALE INPQSSVIL CHIGIVQHA LKQTEKALK 
      TFNVAIANN PKSPLCKFH RGSIYFALG RHAEALKEL 
      EELKEIVPK ESLVYYLIG KVHKKLGNT DLALMHFSW 
      ATDLDPKGA SSQIKEAFD PSIGRTTSS ESPTSPAAE 
      EFLADRRPG LQPEALNFG NLPDDSEDS F
    CDS sequence
    • >TC000032 RA
      atgattgta caggaacca gtgcaggct gccatttgg 
      cattgtttg aatcattat gactacact gatgctgta 
      ttcttgtca gagcgatta tatgctgaa gtcaaatct 
      gatgatagt ctgtacctc ttggccaca gcttattac 
      agaagcggc caaaaagac cacgcatac cacattttg 
      aaggaaagg acagatgct tcaacacag tgcagatat 
      ttgcttggg atttgtgct tatgatctt gaaaaatat 
      gctgaggcc gaggcagct ttgttacac tccaataag 
      agcagcaat gactccgag aatttcgac gacatcaca 
      tcagaatac ggtgatcaa gcaccattc gctttatct 
      ttattaggt aacatagct gccaaaacc gaaagaaaa 
      cctcgtgcc atagatgca tggaaacga gccttaaaa 
      ctaaatcca tttcaatgg tcaagcttc gaaaattta 
      tgcaaaata ggagataaa ccaaacccc caaaatata 
      ttccaaata acaggtgtt gaaaattta tcaatgtgc 
      cagggaagt aatattaac aatatcgaa agtgttgtt 
      ataacaaac aacaatcca aatcaagat aatcaagag 
      acatacgcc acaactcct caacagatt cttgtgaat 
      tttagcccc aatgtcgta acttcgaat tcgaaattg 
      tgtacacct gatgataat ccaattgtt acacaaaat 
      gtgtggctg tcgggcttc gcccccttc acgtcgagg 
      accaagttg ctgagattt cggacggat tcctccagt 
      aatccgagt cctcagccg agctttggc cacttcttg 
      gacacctcc cccgacggt tacttcagc acaccgaac 
      ctcctatcc caaccaaaa ctgaccgaa tccaacaac 
      caccaccaa tcgctagca aagcgcgtg aaagcgcaa 
      gtcgatcaa ttcatcaat cgcaaagaa ccgatcctc 
      caaaactgc aaaccccta ttcagccaa tcgacaaac 
      gtcaccaaa accccaaca atctccctt gccgtacag 
      ccatgtcaa aacgtcaga cgcagctca cgcctcttc 
      accaacagc tactcagtc aaagaaaac aacaaatcg 
      cccaatcga accaaattc gccacaccc aagtcgccc 
      tcgcggaaa accaaacaa cgaatggcg aaatgcaat 
      ttaaacaaa accacagcc tacaccgaa ctcaacaca 
      cgcaaccgc atcgagaag gaaaaaacc gaaacggtg 
      acttcgacc gacgcaaaa atcgagaaa agtgcaata 
      agtaatagt agtagtgtc gagagttgc gtccagcag 
      gcgattcta atgcagaag caaagtgcc gaagggctg 
      atggtcctc ttgcggagt ttaggtcag gcgtacttg 
      catttgtcg aattttaat tgcaaggcg gcgattgaa 
      gaattgaac gttctgccg ccgaatcag ttccaaacg 
      gcttggatt tattgtctt ctggggtta gcctacttc 
      gaattgacc gactacgag tccagcata aagtacttc 
      agtaaagtg cacaacctt gaaccgtac cggatacag 
      ttcatggac gtctactcc acagcgttg tggcacttg 
      cagaaggag gtggcgttg tcagcgctg gcccaggac 
      ctcatctct ttgaacaaa aactcgccg gtgacgtgg 
      tgcgtcagc ggaaactgc ttctcgttg cataaggag 
      cacgacacc gcgattaag tttttccaa agggctgtg 
      caagtcgat ccgaggttt ccctacgcg tatacgctg 
      ctagggcat gagtacatc acgacggag gagcttgac 
      aaggcgatg agttgcttc cgcaatgcc ataagactg 
      gacccaaga cattacaat gcctggttt ggtatcgga 
      accatttac tcaaaacag gagaggtac catctggcg 
      gaaataaac tactcgcga gcgcttgag atcaacccc 
      cagagctcg gtgattctt tgccacatt ggtatcgtc 
      caacacgcg ttgaagcag acggagaag gctctgaag 
      acgtttaac gtggccatt gcgaataat ccgaagagt 
      ccgctgtgt aagttccac cgggggtcg atttatttc 
      gccctcggg aggcacgca gaggcctta aaggagctg 
      gaggagttg aaggagatc gttcccaaa gagtcgcta 
      gtttactat ttaataggc aaagttcac aagaagtta 
      ggaaacacg gatttagcg ctaatgcat ttcagttgg 
      gcgacggat ttggacccg aaaggggcg agtagtcag 
      atcaaggag gcgtttgat ccgtcgatc ggtcgcact 
      acgtccagt gagtcgccc acttcgcca gcggcggag 
      gagtttctg gcggatagg aggccgggc ttgcagccg 
      gaagcgttg aacttcggg aatcttcca gacgatagc 
      gaggatagt ttctaa
    mRNA sequence
    • >TC000032 RA
      ctttttgga aaaatagca aaaaagtca atttttctt 
      gtatttatt ttttacaag cgattttcc actctttgg 
      ttggttttg tgcggttgg ctgcctttg tttataaat 
      tgaattgat cgaagtaaa atggccgtt gattttgcg 
      tttgtgatc atatgtcaa acaaatcaa aaatagtgt 
      tgaattaag aagtgttgc atttatttt aatcatttg 
      tatgcataa ccaatgttt aggcttcaa ttcaactgt 
      aatcaatga agtgaaact ctgaggtaa aaaatgatt 
      gtacaggaa ccagtgcag gctgccatt tggcattgt 
      ttgaatcat tatgactac actgatgct gtattcttg 
      tcagagcga ttatatgct gaagtcaaa tctgatgat 
      agtctgtac ctcttggcc acagcttat tacagaagc 
      ggccaaaaa gaccacgca taccacatt ttgaaggaa 
      aggacagat gcttcaaca cagtgcaga tatttgctt 
      gggatttgt gcttatgat cttgaaaaa tatgctgag 
      gccgaggca gctttgtta cactccaat aagagcagc 
      aatgactcc gagaatttc gacgacatc acatcagaa 
      tacggtgat caagcacca ttcgcttta tctttatta 
      ggtaacata gctgccaaa accgaaaga aaacctcgt 
      gccatagat gcatggaaa cgagcctta aaactaaat 
      ccatttcaa tggtcaagc ttcgaaaat ttatgcaaa 
      ataggagat aaaccaaac ccccaaaat atattccaa 
      ataacaggt gttgaaaat ttatcaatg tgccaggga 
      agtaatatt aacaatatc gaaagtgtt gttataaca 
      aacaacaat ccaaatcaa gataatcaa gagacatac 
      gccacaact cctcaacag attcttgtg aattttagc 
      cccaatgtc gtaacttcg aattcgaaa ttgtgtaca 
      cctgatgat aatccaatt gttacacaa aatgtgtgg 
      ctgtcgggc ttcgccccc ttcacgtcg aggaccaag 
      ttgctgaga tttcggacg gattcctcc agtaatccg 
      agtcctcag ccgagcttt ggccacttc ttggacacc 
      tcccccgac ggttacttc agcacaccg aacctccta 
      tcccaacca aaactgacc gaatccaac aaccaccac 
      caatcgcta gcaaagcgc gtgaaagcg caagtcgat 
      caattcatc aatcgcaaa gaaccgatc ctccaaaac 
      tgcaaaccc ctattcagc caatcgaca aacgtcacc 
      aaaacccca acaatctcc cttgccgta cagccatgt 
      caaaacgtc agacgcagc tcacgcctc ttcaccaac 
      agctactca gtcaaagaa aacaacaaa tcgcccaat 
      cgaaccaaa ttcgccaca cccaagtcg ccctcgcgg 
      aaaaccaaa caacgaatg gcgaaatgc aatttaaac 
      aaaaccaca gcctacacc gaactcaac acacgcaac 
      cgcatcgag aaggaaaaa accgaaacg gtgacttcg 
      accgacgca aaaatcgag aaaagtgca ataagtaat 
      agtagtagt gtcgagagt tgcgtccag caggcgatt 
      ctaatgcag aagcaaagt gccgaaggg ctgatggtc 
      ctcttgcgg agtttaggt caggcgtac ttgcatttg 
      tcgaatttt aattgcaag gcggcgatt gaagaattg 
      aacgttctg ccgccgaat cagttccaa acggcttgg 
      atttattgt cttctgggg ttagcctac ttcgaattg 
      accgactac gagtccagc ataaagtac ttcagtaaa 
      gtgcacaac cttgaaccg taccggata cagttcatg 
      gacgtctac tccacagcg ttgtggcac ttgcagaag 
      gaggtggcg ttgtcagcg ctggcccag gacctcatc 
      tctttgaac aaaaactcg ccggtgacg tggtgcgtc 
      agcggaaac tgcttctcg ttgcataag gagcacgac 
      accgcgatt aagtttttc caaagggct gtgcaagtc 
      gatccgagg tttccctac gcgtatacg ctgctaggg 
      catgagtac atcacgacg gaggagctt gacaaggcg 
      atgagttgc ttccgcaat gccataaga ctggaccca 
      agacattac aatgcctgg tttggtatc ggaaccatt 
      tactcaaaa caggagagg taccatctg gcggaaata 
      aactactcg cgagcgctt gagatcaac ccccagagc 
      tcggtgatt ctttgccac attggtatc gtccaacac 
      gcgttgaag cagacggag aaggctctg aagacgttt 
      aacgtggcc attgcgaat aatccgaag agtccgctg 
      tgtaagttc caccggggg tcgatttat ttcgccctc 
      gggaggcac gcagaggcc ttaaaggag ctggaggag 
      ttgaaggag atcgttccc aaagagtcg ctagtttac 
      tatttaata ggcaaagtt cacaagaag ttaggaaac 
      acggattta gcgctaatg catttcagt tgggcgacg 
      gatttggac ccgaaaggg gcgagtagt cagatcaag 
      gaggcgttt gatccgtcg atcggtcgc actacgtcc 
      agtgagtcg cccacttcg ccagcggcg gaggagttt 
      ctggcggat aggaggccg ggcttgcag ccggaagcg 
      ttgaacttc gggaatctt ccagacgat agcgaggat 
      agtttctaa atgataaac accaagcga tttgttgat 
      tgagttgcg gttttattc atttctaat tgaaggaat 
      actgatttg tgacgtgcc agaggtggt ttgcgtgta 
      tctttaaat tgtgaaatg taaaagatg tgttcgtaa 
      attataggt ttgtcatta aacgatata agaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY