Status
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Gene information
General information
TC006879 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451819.1: 5215..6755
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006879
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04232

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006879 RA
      MANADSVEE LLKKTPKEP DFVIDGIHF GRGGSYYDE 
NTSTHLRHK TTEVKCWRI VGEKLEAIE SKNEFFSSE 
TYIVRWKSN TEYLRFEEV LSTRDNEIY FYWKGEDAP 
KGHSPLPEC IEKDNPPVE RIVQWAEPP AFLQLFSGK 
FVVHIGKHS LLSKAPHLY ILRGELEEE LHLYELPLK 
RGNLRSRTS FVVFNPDEK SSIIWHGKN SNAHCKESI 
RKATEKIFG PKYTEITEG SENDKLIYL VENEDCFNA 
PLKLIDYTP RLFYFNKIT GSFLTTEID CSHNSSHIT 
PFPFLQSHL YTPEQPAIF LLDDNDEIW IWYGWDQDE 
DDGFKTECF IMAINYAKQ KSKNIKRLV KLQKVIAGF 
EPDSFTHLF PVWQKRQDI AQLQEKAGV LLSANPLIE 
VISPEEAPD T
    • >TC006879 RB
      MANADSVEE LLKKTPKEP DFVIDGIHF GRGGSYYDE 
NTSTHLRHK TTEVKCWRI VGEKLEAIE SKNEFFSSE 
TYIVRWKSN TEYLRFEEV LSTRDNEIY FYWKGEDAP 
KGHSPLPEC IEKDNPPVE RIVQWAEPP AFLQLFSGK 
FVVHIGKHS LLSKAPHLY ILRGELEEE LHLYELPLK 
RGNLRSRTS FVVFNPDEK SSIIWHGKN SNAHCKESI 
RKATEKIFG PKYTEITEG SENDKLIYL VENEDCFNA 
PLKLIDYTP RLFYFNKIT GSFLTTEID CSHNSSHIT 
PFPFLQSHL YTPEQPAIF LLDDNDEIW IWYGWDQDE 
DDGFKTECF IMAINYAKQ KSKNIKRLV KLQKVIAGF 
EPDSFTHLF PVWQKRQDI AQLQEKVGI EDSAFSFCC 
KAGVLLSAN PLIEVISPE EAPDT
    CDS sequence
    • >TC006879 RA
      atggcaaat gcagattct gttgaagag ttgcttaaa 
aaaacgcca aaagagcca gattttgta atagacggc 
atacacttt ggccgaggt ggttcctac tacgacgaa 
aacacctcc acgcatttg agacacaaa acgaccgaa 
gtgaaatgt tggcgaatc gttggagaa aagttggaa 
gcgattgaa tcaaaaaat gaatttttt tcttccgag 
acttatata gtacggtgg aaatcaaac acggaatat 
ttacggttt gaggaagtt ttgagtaca agggataat 
gagatttat ttctattgg aaaggagaa gacgctcct 
aaaggacat tcaccgttg cctgaatgt atagagaag 
gacaatccg ccggttgaa cgaattgtc caatgggct 
gaaccaccg gctttcctt cagctattt tcgggaaag 
tttgttgtt cacattgga aaacacagt ttgttaagt 
aaggcccct cacttgtac atcctcaga ggcgagctc 
gaggaggaa ctacattta tatgaactg ccgctaaag 
cgaggaaat cttcggagt agaacctca tttgtggtt 
tttaacccc gacgaaaaa tcatccatt atttggcac 
ggaaaaaac tcaaacgcg cattgtaaa gaatcgatc 
cgaaaagcg accgaaaaa atatttggc cccaaatat 
accgaaatt accgaagga tctgaaaat gacaaactc 
atttattta gtagaaaat gaagattgt tttaatgcc 
ccgctcaaa cttatcgac tatacgcca agattgttt 
tatttcaat aaaatcacg gggagtttt ttaaccact 
gaaatagac tgtagtcac aacagttcg cacataact 
ccgtttccg tttcttcag tcacacctg tatactccg 
gagcaacca gcaattttt ctcttggac gacaacgac 
gaaatttgg atctggtac gggtgggat caggacgag 
gatgacggc tttaaaact gagtgcttt atcatggcc 
attaattat gccaaacag aagagcaaa aatatcaag 
cgcttggtc aagcttcag aaagtgatt gccggtttt 
gagccggat tcatttacg catttattt cccgtttgg 
caaaaaaga caagacatc gcacaacta caagaaaag 
gctggtgtg ttgctaagt gcaaatcct ttgattgaa 
gtcatctcg ccagaggag gctccagac acataa
    • >TC006879 RB
      atggcaaat gcagattct gttgaagag ttgcttaaa 
aaaacgcca aaagagcca gattttgta atagacggc 
atacacttt ggccgaggt ggttcctac tacgacgaa 
aacacctcc acgcatttg agacacaaa acgaccgaa 
gtgaaatgt tggcgaatc gttggagaa aagttggaa 
gcgattgaa tcaaaaaat gaatttttt tcttccgag 
acttatata gtacggtgg aaatcaaac acggaatat 
ttacggttt gaggaagtt ttgagtaca agggataat 
gagatttat ttctattgg aaaggagaa gacgctcct 
aaaggacat tcaccgttg cctgaatgt atagagaag 
gacaatccg ccggttgaa cgaattgtc caatgggct 
gaaccaccg gctttcctt cagctattt tcgggaaag 
tttgttgtt cacattgga aaacacagt ttgttaagt 
aaggcccct cacttgtac atcctcaga ggcgagctc 
gaggaggaa ctacattta tatgaactg ccgctaaag 
cgaggaaat cttcggagt agaacctca tttgtggtt 
tttaacccc gacgaaaaa tcatccatt atttggcac 
ggaaaaaac tcaaacgcg cattgtaaa gaatcgatc 
cgaaaagcg accgaaaaa atatttggc cccaaatat 
accgaaatt accgaagga tctgaaaat gacaaactc 
atttattta gtagaaaat gaagattgt tttaatgcc 
ccgctcaaa cttatcgac tatacgcca agattgttt 
tatttcaat aaaatcacg gggagtttt ttaaccact 
gaaatagac tgtagtcac aacagttcg cacataact 
ccgtttccg tttcttcag tcacacctg tatactccg 
gagcaacca gcaattttt ctcttggac gacaacgac 
gaaatttgg atctggtac gggtgggat caggacgag 
gatgacggc tttaaaact gagtgcttt atcatggcc 
attaattat gccaaacag aagagcaaa aatatcaag 
cgcttggtc aagcttcag aaagtgatt gccggtttt 
gagccggat tcatttacg catttattt cccgtttgg 
caaaaaaga caagacatc gcacaacta caagaaaag 
gttggtatt gaagatagt gctttttcg ttttgttgt 
aaggctggt gtgttgcta agtgcaaat cctttgatt 
gaagtcatc tcgccagag gaggctcca gacacataa
    mRNA sequence
    • >TC006879 RA
      tcagtgttt taatcggtt agttgaagt caagcaaga 
tttaattac acttgtggt tttcttgtg cgataaaca 
ttcgcggaa aggtatggc aaatgcaga ttctgttga 
agagttgct taaaaaaac gccaaaaga gccagattt 
tgtaataga cggcataca ctttggccg aggtggttc 
ctactacga cgaaaacac ctccacgca tttgagaca 
caaaacgac cgaagtgaa atgttggcg aatcgttgg 
agaaaagtt ggaagcgat tgaatcaaa aaatgaatt 
tttttcttc cgagactta tatagtacg gtggaaatc 
aaacacgga atatttacg gtttgagga agttttgag 
tacaaggga taatgagat ttatttcta ttggaaagg 
agaagacgc tcctaaagg acattcacc gttgcctga 
atgtataga gaaggacaa tccgccggt tgaacgaat 
tgtccaatg ggctgaacc accggcttt ccttcagct 
attttcggg aaagtttgt tgttcacat tggaaaaca 
cagtttgtt aagtaaggc ccctcactt gtacatcct 
cagaggcga gctcgagga ggaactaca tttatatga 
actgccgct aaagcgagg aaatcttcg gagtagaac 
ctcatttgt ggtttttaa ccccgacga aaaatcatc 
cattatttg gcacggaaa aaactcaaa cgcgcattg 
taaagaatc gatccgaaa agcgaccga aaaaatatt 
tggccccaa atataccga aattaccga aggatctga 
aaatgacaa actcattta tttagtaga aaatgaaga 
ttgttttaa tgccccgct caaacttat cgactatac 
gccaagatt gttttattt caataaaat cacggggag 
ttttttaac cactgaaat agactgtag tcacaacag 
ttcgcacat aactccgtt tccgtttct tcagtcaca 
cctgtatac tccggagca accagcaat ttttctctt 
ggacgacaa cgacgaaat ttggatctg gtacgggtg 
ggatcagga cgaggatga cggctttaa aactgagtg 
ctttatcat ggccattaa ttatgccaa acagaagag 
caaaaatat caagcgctt ggtcaagct tcagaaagt 
gattgccgg ttttgagcc ggattcatt tacgcattt 
atttcccgt ttggcaaaa aagacaaga catcgcaca 
actacaaga aaaggctgg tgtgttgct aagtgcaaa 
tcctttgat tgaagtcat ctcgccaga ggaggctcc 
agacacata acccaaaac agtttgtta tatattgca 
taaaaatgg tgataaggg tttaattat agtcgtatt 
tatgcgatt tgagatttt tctaatata actttttta 
taccg
    • >TC006879 RB
      tcagtgttt taatcggtt agttgaagt caagcaaga 
tttaattac acttgtggt tttcttgtg cgataaaca 
ttcgcggaa aggtatggc aaatgcaga ttctgttga 
agagttgct taaaaaaac gccaaaaga gccagattt 
tgtaataga cggcataca ctttggccg aggtggttc 
ctactacga cgaaaacac ctccacgca tttgagaca 
caaaacgac cgaagtgaa atgttggcg aatcgttgg 
agaaaagtt ggaagcgat tgaatcaaa aaatgaatt 
tttttcttc cgagactta tatagtacg gtggaaatc 
aaacacgga atatttacg gtttgagga agttttgag 
tacaaggga taatgagat ttatttcta ttggaaagg 
agaagacgc tcctaaagg acattcacc gttgcctga 
atgtataga gaaggacaa tccgccggt tgaacgaat 
tgtccaatg ggctgaacc accggcttt ccttcagct 
attttcggg aaagtttgt tgttcacat tggaaaaca 
cagtttgtt aagtaaggc ccctcactt gtacatcct 
cagaggcga gctcgagga ggaactaca tttatatga 
actgccgct aaagcgagg aaatcttcg gagtagaac 
ctcatttgt ggtttttaa ccccgacga aaaatcatc 
cattatttg gcacggaaa aaactcaaa cgcgcattg 
taaagaatc gatccgaaa agcgaccga aaaaatatt 
tggccccaa atataccga aattaccga aggatctga 
aaatgacaa actcattta tttagtaga aaatgaaga 
ttgttttaa tgccccgct caaacttat cgactatac 
gccaagatt gttttattt caataaaat cacggggag 
ttttttaac cactgaaat agactgtag tcacaacag 
ttcgcacat aactccgtt tccgtttct tcagtcaca 
cctgtatac tccggagca accagcaat ttttctctt 
ggacgacaa cgacgaaat ttggatctg gtacgggtg 
ggatcagga cgaggatga cggctttaa aactgagtg 
ctttatcat ggccattaa ttatgccaa acagaagag 
caaaaatat caagcgctt ggtcaagct tcagaaagt 
gattgccgg ttttgagcc ggattcatt tacgcattt 
atttcccgt ttggcaaaa aagacaaga catcgcaca 
actacaaga aaaggttgg tattgaaga tagtgcttt 
ttcgttttg ttgtaaggc tggtgtgtt gctaagtgc 
aaatccttt gattgaagt catctcgcc agaggaggc 
tccagacac ataacccaa aacagtttg ttatatatt 
gcataaaaa tggtgataa gggtttaat tatagtcgt 
atttatgcg atttgagat ttttctaat ataactttt 
ttataccg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY