| General information | |||
| TC031778
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451556.1: 59346..83843 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031778 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04231 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
remarks: animals lost,
Adult morphology
remarks: animals lost,
- >TC031778 RA
MSGTDDELD SDTEAGDIR VGWIYRLRK QELISDLKR FGLSTEGTV EELRRRLRQ FVKENAAAV GTTPEITNT ESTMTAFTT TYVPNVFSF NVPPQAGTV PRGYTGINT STTQVEGWY TRRPATEMP QPTFTYPAP RLSSLETQP VINPVAHLD FVNQIRKWN VTFDGKNDA VSFLERVEE LAEEYMIPK NHLVRALPE LLRNQAILW YRNQKSDIH SWNDFVRVF RAFYFPREY REGLEEEIF RRVQRPQEN GRDFVIQLQ TLIRRHGGW TRDQELNCI YKHLLPEYR QYIRKNDVR SVMELLDQI QEYEGLTQE IRRSKSDVK SCMRDCTRK GSDQSPELG AITGTPYPI GQVFPDFAN NSRVLWKIV VENQEIPAL IDTGSVLSY INEETAKHC ISQGLQPQF LSSRMQAKM ANGVTVDTN LFFNIKFQA SDLEFSCDF LLCPNLTSP MILGLDFIM EQQLVLNFE TGEISRQGQ VLVCVKTPL TLVTSERTD SDVSTAPSE PVETATATV DTAIVGDGF VEREIKNFA EVQGRTDMT VHEIRLTNP TPIKQRYRP QNPAMQQII DEEVDRMLT EDIIEPSES PWSSPIVLA DKKDGRKKR FCIDFRKIN SVSQKDAYP IPHINGILD KLRGAKYFS KIDSKPRIL PKIPPAPEP INLAIPDNH RRLPMGPPA TETEDGGQG DGRIRHYVM PFLKKGGGV MKHPDSGRF YGDVGVMRS SQFHHHRRS VCLHRVANK RAKPSVLQQ LLTVHQTGS PKGPTATRS PTKPPGKRT LQILAPEQG PEGSEGKEA GDIRVGWIY RLRKQELIS DLKRFGLST EGTVEELRR RLRQFVKEN AAAVGTTPE ITNTECTMT AFTTTYVPN VFSFNVPPQ AGTVPRGYT GINTSTTQV EGWYTRRPA TEMPQPTFT YPAPRLSSL ETQPVINPV AHLDFVNQI RKWNVTFDG KNDAVSFLE RVEELAEEY MIPKNHLVR ALPELLRNQ AILWYRNQK SDIHSWNDF VRVFRAFYF PREYREGLE EEIFRRVQR PQENGRDFV IQLQTLIRR HGGWTRDQE LNCIYKHLL PEYRQYIRK NDVRSVMEL LDQIQEYEG LTQEIRRSK SDVKSCMRD CTRKGSDQS PELGAITGT PYPIGQVFP DFANNSRVL WKIVVENQE IPALIDTGS VLSYINEET AKHCISQGL QPQFLSSRM QAKMANGVT VDTNLFFNI KFQASDLEF SCDFLLCPN LTSPMILGL DFIMEQQLI LNFETGEIS RQGQVLVCV KTPLTLVTS ERTDSDVST APSEPVETA TATVDTAIV GDGFVEREI KNFAEVQGR TDMTVHEIR LTNPTPIKQ RYRPQNPAM QQIIDEEVD RMLTEGIIE PSESPWSSP IVLADKKDG RKKRFCIDF RKINSVSQK DAYPIPHIN GILDKLRGA KYFSKIDLK QGYWQVPLA EESKPITAF TVPNRGLFQ FKVMPFGLH SAPATFQRL LDRVLGPEM DSGAFAYLD DIIVLGRTK EEHQENLAT VFRKLRKAK LTPNWEKCS FGQTRLEYL GHIVSSQGV STDPSKVKA ILELPPPTN VKELRRFLG IASWYRKFV PVFSQVAAA LNRLLKKKQ KWEWGNEQK EAFKKIKQL LTEAPVLAC PDFTKTFVL QTDASDLGL GAALIQNLD GGDRVIAYA SCSLSDQQT KYSTTEKEC LAIVWAIQK MRPYIEGYH FVVESDHQP LKWLMKQPE PKGRLARWI MTLQQYDFE VRYRKGVLN QVADALSRN PMPQKSGIE AIETAHPID GWYRQKYEE VSATPDLFP DFKIENDRL YKHIMNSQG LTGAQEWKL CIPKGQRKQ VLFENHDVP TAGHLGIAK TTARLAQKY YWPGMFREA AKYVQSCEN CQRYKSSQQ ARPGLMQPY KIYEPWHTV SVDLVGPLP RSSKGNSYV LVCQDRFTK WIEVQPLRR ATAPVVTRT LYENIITRF GCPKRIITD NGTQLESRL LNKLMKDFH LKHRLTPPY TPQANPVER ANKVLKTMI AQYCEENQR KWDENLCSF RMAINSSKH ESTGFTPAY LNLGHELRM PSALLQEDK NEEIGFDPE TEDAAIQEY PKRIQELQE QFELVRLNL SRAFTTQKK YYDRRRREW SCHPGDRVM KRDHVLSSA ARGFAAKLA PKYSGPYII TKVLSPVME STPPRNRHS ESIETKLVR LFGQSPESP YLELYASPL PGLVSPLPP TPSPVRRRE IQEALIKKL GTSPILASP TAWLESPKT PVQFSPEPE KPVTPGKSI AQEVLDVFR SKPRILPKI PPAPEPINL AVPLWTFRP AGNTEGRCR FRFPTTTGG FRWVLLPPR PKTADKATG ESCAQVSST TTGARFVYT VSPIKGRNR AFSSSCSLC IKPVSNSGG RAVHQTGKT FSDKWCLLR IKPAHHLHR PDRPRAPTS GACCASNQH TISTAQTDL GLGREITTE DNQREYKMS GTDDELDSD TEAGDIRVG WIYRLRKQE LISDLKRFG LSTEGTVEE LRRRLRQFV KENAAAVGT TPEITNTES TMTAFTTTY VPNVFSFNV PPQAGTVPR GYTGINTST TQVEGWYTR RPATEMPQP TFTYPAPRL SSLETQPVI NPVAHLDFV NQIRKWNVT FDGKNDAVS FLERVEELA EEYMIPKNH LVRALPELL RNQAILWYR NQKSDIHSW NDFVRVFRA FYFPREYRE GLEEEIFRR VQRPQENGR DFVIQLQTL IRRHGGWTR DQELNCIYK HLLPEYRQY IRKNDVRSV MELLDQIQE YEGLTQEIR RSKSDVKSC MSTTSNWTK AKPGFSMST PQQTSLQRP KPAPRPTWS SNSNKQSDL ERLCWRCGQ KGHTRLQCR QPAILFCSR CGRQGVKSI ECPCPFKPG NSKGTAPEK GSDQSPELG AITGTPYPI GQVFPDFAN NSRVLWKIV VENQEIPAL IDTGSVLSY INEETAKHC ISQGLQPQF LSSRMQAKM ANGVTVDTN LFFNIKFQA SDLEFSCDF LLCPNLTSP MILGLDFIM EQQLVLNFE TGEISRQGQ VLVCVKTPL TLVTSERTD SDVSTAPSE PVETATATV DTAIVGDGF VEREIKNFA EVQGRTDMT VHEIRLTNP TPIKQRYRP QNPAMQQII DEEVDRMLT EDIIEPSES PWSSPIVLA DKKDGRKKR FCIDFRKIN SVSQKDAYP IPHINGILD KLRGAKYFS KIDLKQGYW QVPLAEESK PITAFTVPN RGLFQFKVM PFGLHSAPA TFQRLLDRV LGPEMDSGA FAYLDDIIV LGRTKEEHQ ENLATVFRK LRKAKLTPN WEKCSFGQT RLEYLGHIV SSQGVSTDP SKVKAILEL PPPTNVKEL RRFLGIASW YRKFVPVFS QVAAAVNRL LKKKQKWEW GNEQKEAFE KIKQLLTEA PVLACPDFT KTFVLQTDA SDLGLGAAL IQNLDGGDR VIAYASCSL SDQQTKYST TEKECLAIV WAIQKMRPY IEGYHFVVE SDHQPLKWL MKQPEPKGR LARWIMTLQ QYDFEVRYR KGVLNQVAD ALSRNPMPQ KSGIEAIET AHPIDRWYR QKYEEVSAT PDLFPDFKI ENDRLYKHI MNSQGLTGA QEWKLCIPK GQRKQVLFE NHDVPTAGH LGIAKTTAR LAQKYYWPG MFREAAKYV QSCENCQRY KSSQQARPG LMQPYKIYE PWHTVSVDL VGPLPRSSK GNSYVLVCQ DRFTKWIEV QPLRRATAP VVTRTLYEN IITRFGCPK RIITDNGTQ LESRLLNKL MKDFHIKHR LTPPYTPQA NPVERANKV LKTMIAQYC EENQRKWDE NLCSFRMAI NSSKHESTG FTPAYLNLG HELRMPSAL LQEDKNEEI GFDPETEDA AIQEYPKRI QELQEQFEL VRLNLSRAF TTQKKYYDR RRREWSCHP GDRVMKRDH VLSSAARGF AAKLAPKYS GPYIITKVL SPVMESTPP RNRHSESIE TKLVRLFGQ SPESPYLEL YASPLPGLV SPLPPTPSP VRRREIQEA LIKKLGTSP ILASPTAWL ESPKTPVQF SPEPEKPVT PGKSIAQEV LDVFRSKPR ILPKIPPAP EPINLAIPD NHRRLPMGP PATETEDGG QGDGRIRHY VMPFLKKGG GVMKHPDSG RFYGDVGVM RSRRNRAFS SSCSLCIKP VSNSGGRAV HQTGKTFSD EWCLLRIKP AHHLHRPDR PRAPTSGAC CASNQHTIS TAQTDLGLG REITTEDNR FPKGPTATR SPTKPPGKR TLQIFAEVQ GRTDMTVHE IRLTNPTPI KQRYRPQNP AMQQIIDEE VDRMLTEDI IEPSESPWS SPIVLADKK DGRKKRFCI DFRKINSVS QKDAYPIPH INGILDKLR GAKYFSKID SKPRILPKI PPAPEPINL AIPDNHRRL PMGPPATET EDGGQGDGR IRHYVMPFL KKGGGVMKH PDSGRFYGD VGVMRSRRN RAFSSSCSL CIKPVSNSG GRAVHQTGK TFSDEWCLL RIKPAHHLH RPDRPRAPT SGACCASNQ HTISTDPTD LGLGFPKGP TATRSPTKP PGKRTLQIL APEQGQVLV CVKTPLTLV TSERTDSDV STAPSEPVE TATATVDTA IVGDGFVER EIKNFAEVQ GRTDMTVHE IRLTNPTPI KQRYRPQNP AMQQIIDEE VDRMLTEDI IEPSESPWS SPIVLADKK DGRKKRFCI DFRKINSVS QKDAYPIPH INGILDKLR GAKYFSKID LKQGYWQVP LAEEILLYT HIEDTPVHR L
- >TC031778 RA
atgtctggc accgacgac gaattggat tccgacaca gaagctgga gacataaga gttggatgg atctaccgt ttgaggaaa caggaacta atcagtgac ttgaagaga tttggactc tccaccgaa ggtaccgtt gaggaacta cgacgtcgt ctccgacag tttgtcaaa gagaatgcg gccgcagtg ggaacaact ccggaaata accaacacg gagtctacg atgacggct ttcacgacg acctatgtt ccgaacgta ttctccttc aacgtaccg ccccaagcc ggaactgtt cccagaggg tacaccgga atcaacacc tccacaacc caggttgaa ggatggtat acgagacga cctgccacc gaaatgcca cagccgacg ttcacgtac cccgctcct agactatcg tcgttagaa acacaacca gtgattaat ccggtagct cacctggat tttgtgaat caaatccgg aagtggaac gttactttc gatggtaag aatgatgca gtttctttc ctagaacgt gttgaggaa ctggccgaa gaatacatg ataccaaag aaccatcta gtacgggca ttaccggaa ttactccgg aatcaagca atcctgtgg taccggaat cagaaaagc gacattcac tcttggaac gatttcgtc agggttttc agagcattt tattttcct cgagagtac cgtgagggc cttgaagaa gaaatcttc cggagggtg cagagaccg caggagaat ggccgtgac ttcgtaatc cagttgcaa actctgatc cggcgacac ggaggatgg acaagggac caagaactg aattgtata tacaaacac ttgctccca gagtaccga caatacata cgcaaaaac gacgtccgc tccgtaatg gaattattg gaccaaatc caggaatac gaagggttg actcaggaa ataagacgg tcgaaatca gacgtgaag agctgcatg agggactgc acccgaaaa ggctccgac cagtccccg gaactagga gccattacc ggcacaccg tacccgatt ggacaagtt ttccccgat tttgcgaat aatagccga gtcttgtgg aaaattgtg gtagaaaac caagaaatt ccggccctg attgacacc gggtccgtg ctctcctac atcaacgaa gagacggcc aaacattgt ataagtcaa ggactgcag ccacaattt ttatcctcc aggatgcaa gccaagatg gcaaatggg gtaaccgtc gatacaaac ttgttcttt aacatcaaa ttccaggca agtgactta gaattttcc tgtgatttt ctgctctgt ccgaactta actagtcca atgatcttg gggttggac tttatcatg gagcaacag ctagtatta aattttgaa accggagaa atttctcga cagggacaa gtcttagtc tgcgtaaag acaccgcta actcttgtg accagcgaa aggaccgat tccgacgtc agcaccgcc ccttccgaa cctgttgaa accgcaaca gcgacagtg gataccgcc attgttggg gacggtttt gttgaacgt gaaattaaa aatttcgct gaggtccaa gggagaaca gatatgact gtccacgaa atccgtctc acaaacccg actccgata aaacaaagg taccgaccc caaaatccg gcaatgcag cagattatc gatgaggaa gttgaccgg atgttaaca gaagacatc atcgaacca tccgagagc ccgtggagt tctccaatt gtcctagcc gacaaaaag gatgggaga aagaagaga ttctgtatc gatttccgt aagatcaat tccgtatcg caaaaggat gcatacccg attccacac attaatggg atcttagac aaattacgt ggggcgaaa tatttttct aaaattgat tcgaagcca aggatactg ccgaaaatt ccaccggcg ccggaaccg atcaacctg gcaattccc gacaaccac cggcggctt ccgatgggt cctcctgcc accgagacc gaagacggc ggacaaggc gacgggcgt atccggcat tacgtcatg ccgtttctg aagaaaggg gggggtgta atgaagcat ccggattcc ggacgattc tatggtgac gtaggagtc atgcgctca agtcagttc caccaccac cggcgctcg gtttgttta caccgtgtc gccaataaa agggcgaaa ccgagcgtt ctccagcag ttgctcact gtgcatcaa accggctct ccaaaaggc ccaaccgca acccgatct ccgacaaag ccgcccgga aaaaggaca ttacaaatt ttggcgccc gagcaggga cctgaaggg tctgaagga aaagaagct ggagacata agagttgga tggatctac cgtttgagg aaacaggaa ctaatcagt gacttgaag agatttgga ctctccacc gaaggtact gttgaggaa ctacgacgt cgtctccga cagtttgtc aaagagaat gcggccgca gttggaaca actccggaa ataaccaac acggagtgt acgatgacg gctttcacg acgacctat gttccgaac gtattctcc ttcaacgta ccgccccaa gccggaact gttcccaga gggtacacc ggaatcaac acctccaca acccaggtg gaaggatgg tatacgaga cgacctgcc accgaaatg ccacagccg acgttcacg taccccgct cctagacta tcgtcgtta gaaacacaa ccagtgatt aatccggta gctcacctg gattttgtg aatcaaatc cggaagtgg aacgttact ttcgatggt aagaatgat gcagtttct ttcttagaa cgtgttgag gaactggcc gaagaatac atgataccg aagaaccat ctagtacgg gcattaccg gaattactc cggaatcaa gcaatcctg tggtaccgg aatcagaaa agcgacatt cactcttgg aacgatttc gtcagggtt ttcagagca ttttatttt cctcgagag taccgtgag ggccttgaa gaagaaatc ttccggagg gtgcagaga ccgcaggag aatggccgt gacttcgta atccagttg caaactctg atccggcga cacggagga tggacaagg gaccaagaa ctgaattgt atatacaaa cacttgctc ccagagtac cgacaatac atacgcaag aacgacgtc cgctccgta atggaatta ttggaccaa atccaggaa tacgaaggg ttgactcag gaaataaga aggtcgaaa tcagacgtg aagagctgc atgagggac tgcacccga aaaggctcc gaccagtcc ccggaacta ggagccatt accggcaca ccgtacccg attggacaa gttttcccc gattttgcg aataatagc cgagtcttg tggaaaatt gtggtagaa aaccaagaa attccggcc ctgattgac accgggtcc gtgctctcc tacatcaac gaagagacg gccaaacat tgtataagt caaggactg cagccacaa tttttatcc tccaggatg caagccaag atggcaaat ggggtaacc gtcgataca aacttgttc tttaacatc aaattccag gcaagtgac ttagaattt tcctgtgat tttctgctc tgtccgaac ttaactagt ccaatgatc ttggggttg gactttatc atggagcaa cagctaata ttaaatttt gaaaccgga gaaatttct cgacaggga caagtctta gtctgcgta aagacaccg ctaactctt gtgaccagc gaaaggacc gattccgac gtcagcacc gccccttcc gaacctgtt gaaaccgca acagcgaca gtggatacc gccattgtt ggggacggt tttgttgaa cgtgaaatt aaaaatttc gctgaggtc caagggaga acagatatg actgtccac gaaatccgt ctcacaaac ccgactccg ataaaacaa aggtaccga ccccaaaat ccggcaatg cagcagatt atcgatgag gaagttgac cggatgtta acagaaggc atcatcgaa ccatccgag agcccgtgg agttctcca attgtccta gccgacaaa aaggatggg agaaagaag agattctgt atcgatttc cgtaagatc aattccgta tcgcaaaag gatgcatac ccgattcca cacattaat gggatctta gacaaatta cgtggggcg aaatatttt tctaaaatt gatttgaaa caaggatat tggcaagtt cctctcgcc gaagaaagt aagcccata acagctttc accgtgccg aaccgggga ttatttcaa ttcaaagtt atgccattt ggtctccat tccgcacca gcaacgttc caaagacta ttagaccgg gtcctgggt ccggaaatg gacagtggt gcattcgcc tatttggat gacatcatt gtgctggga cggaccaaa gaagagcac caagagaac ttggcaaca gtcttccgt aagctaaga aaggcaaag ctgacacca aattgggaa aaatgctct tttggtcaa acaaggctt gaatatctg ggacatatt gtctcatca cagggagtt agcaccgat ccctcgaaa gtgaaagcc atcctggaa ttaccacca ccaactaat gtcaaggaa cttcgtcga tttctagga atagcctcc tggtaccgg aagttcgtg cccgtattt tctcaagtc gccgccgca ttaaaccga cttctgaag aagaagcag aaatgggaa tggggaaac gaacagaag gaagcattc aagaaaatc aaacaacta ctcaccgaa gcacccgtt ctggcctgc cccgacttc acaaagact tttgtttta cagaccgac gctagtgac ctaggactt ggtgcggcc ctcatacag aacctcgat ggaggcgac cgggtgatc gcctatgct agttgtagt ctttcggat caacaaacg aaatactca acgacagag aaagaatgt ttggcaatt gtgtgggcg atccagaaa atgaggccg tacatcgag ggataccac ttcgtcgta gagtccgac caccagccc ctgaagtgg ctgatgaaa caaccagaa cccaaaggc cgcctagcg agatggatc atgacgtta caacaatat gattttgag gtgcgatac cggaaggga gtgttgaat caagtcgcc gacgcccta tctcgtaac ccgatgccg cagaaatcc gggatagag gcgattgag actgctcac cccatcgac ggatggtac cggcaaaaa tatgaagag gtatcagct accccagat ctctttccg gactttaag attgaaaac gaccgatta tacaaacac attatgaac agccaggga ctcaccgga gcgcaagaa tggaaattg tgtatcccg aaaggtcaa agaaagcaa gtcttgttt gaaaatcac gacgtccca accgctggt catttagga atcgcaaag acaactgca agacttgca caaaaatat tattggccc ggaatgttt cgagaagcc gcaaaatat gtccagagc tgtgaaaac tgtcaacgt tacaagtcc agccaacaa gcgagaccc ggactaatg caaccctac aaaatatac gaaccgtgg cacaccgta agtgtggac ttggtcggc ccacttccc cgttcatca aaaggcaac agctacgta ctcgtctgc caagacagg tttacaaaa tggatagaa gttcagccc ttgcgtcgg gccaccgca cctgtcgtg acaagaact ttatacgaa aatataatt actcgattt gggtgcccg aaaagaatc ataaccgac aacggaact caactggag agccgactt ctaaataaa ctgatgaaa gacttccac ttaaagcac cgattaaca ccgccttac acaccgcag gcaaatcca gtggaacga gcgaacaaa gtgttaaag acaatgata gcccaatac tgtgaagaa aaccaaagg aaatgggac gaaaatctt tgcagtttc cgcatggct atcaactcg tcaaaacat gagtccact ggattcacc ccagcctat ttgaactta gggcacgaa ctccggatg ccttcggcg ctactacaa gaagacaaa aatgaggaa atcggattt gacccggaa acagaggac gccgcgatc caggaatac ccgaaaagg attcaggag ctacaagaa cagttcgaa ctagtacga ctaaacctt tctagggca ttcaccacg caaaagaag tactatgac cgccgccgt cgagagtgg tcatgtcac ccaggtgac cgcgttatg aaacgagat cacgtgttg tcttcggcc gctcgaggg ttcgctgcc aagctcgct cccaaatat tccggcccg tacataatc accaaagtc ctctctcct gtaatggaa tcaacaccg ccgagaaac cgccactcc gagtccatc gagacaaaa ttggtgcgc ctgtttgga caatccccg gaaagcccc tatctggag ctatatgca tccccatta ccaggtctc gtgtcgccg ctcccgcca acaccgtcc ccagtccgc agaagggag attcaggag gcccttatt aagaagctg ggaacaagc cccatattg gcaagcccg acagcctgg ctggaatcc ccgaaaact ccggtgcag ttttccccg gaaccggaa aaacccgtt acccctggc aaaagcatc gcccaggag gttctggac gttttccgg tcgaagcca aggatactg ccgaaaatt ccaccggcg ccggaaccg atcaacctg gcagtccca ctttggact ttccggcca gcgggtaat acagagggg cgatgccga ttcagattc ccgacaacc accggcggc ttccgatgg gtcctcctg ccaccgaga ccgaagacg gcggacaag gcgacgggc gagtcatgc gctcaagtc agttccacc accaccggc gctcggttt gtttacacc gtgtcgcca ataaaaggg cgaaaccga gcgttctcc agcagttgc tcactgtgc atcaaaccg gtgagtaac tctgggggt cgagccgtg catcaaacc ggcaaaacc ttctccgac aagtggtgc ttgctgcgc atcaaacca gcacaccat ctccaccga cccgaccga cctagagct ccgacgagt ggtgcttgc tgcgcatca aaccagcac accatctcc accgcccag accgacctg gggcttggt agggaaatt acgaccgag gacaaccag agagagtac aagatgtct ggcaccgac gacgaattg gattccgac acagaagct ggagacata agagttgga tggatctac cgtttgagg aaacaggaa ctaatcagt gacttgaag agatttgga ctctccacc gaaggtact gttgaggaa ctacgacgt cgtctccga cagtttgtc aaagagaat gcggccgca gttggaaca actccggaa ataaccaac acggagtct acgatgacg gctttcacg acgacctat gttccgaac gtattctcc ttcaacgta ccgccccaa gccggaact gttcccaga gggtacacc ggaatcaac acctccaca acccaggtt gaaggatgg tatacgaga cgacctgcc accgaaatg ccacagccg acgttcacg taccccgct cctagacta tcgtcgtta gaaacacaa ccagtgatt aatccggta gctcacctg gattttgtg aatcaaatc cggaagtgg aacgttact ttcgatggt aagaatgat gcagtttct ttcctagaa cgtgttgag gaactggcc gaagaatac atgataccg aagaaccat ctagtacgg gcattaccg gaattactc cggaatcaa gcaatcctg tggtaccgg aatcagaaa agcgacatt cactcttgg aacgatttc gtcagggtt ttcagagca ttttatttt cctcgagag taccgtgag ggccttgaa gaagaaatc ttccggagg gtgcagaga ccgcaggag aatggccgt gacttcgta atccagttg caaactctg atccggcga cacggagga tggacaagg gaccaagaa ctgaattgt atatacaaa cacttgctc ccagagtac cgacaatac atacgcaaa aacgacgtc cgctccgta atggaatta ttggaccaa atccaggaa tacgaaggg ttgactcag gaaataaga cggtcgaaa tcagacgtg aagagctgc atgagtacc acatcaaat tggacaaag gcaaagccc ggattttcg atgagcact ccgcaacaa acgtcatta caaagacca aaaccggca ccaagaccc acatggagc tcgaacagc aacaaacag tctgattta gaaaggtta tgttggaga tgcggtcaa aagggccac acacgccta caatgccga caaccagca atactgttc tgctcaaga tgtggacgc caaggggta aaatcgatc gagtgtccc tgccccttt aaaccggga aattcaaag gggactgca cccgaaaaa ggctccgac cagtccccg gaactagga gccattacc ggcacaccg tacccgatt ggacaagtt ttccccgat tttgcgaat aatagccga gtcttgtgg aaaattgtg gtagaaaac caagaaatt ccggccctg attgacacc gggtccgtg ctctcctac atcaacgaa gagacggcc aaacattgt ataagtcaa ggactgcag ccacaattt ttatcctcc aggatgcaa gccaagatg gcaaatggg gtaaccgtc gatacaaac ttgttcttt aacatcaaa ttccaggca agtgactta gaattttcc tgtgatttt ctgctctgt ccgaactta actagtcca atgatcttg gggttggac tttatcatg gagcaacag ctagtatta aattttgaa accggagaa atttctcga cagggacaa gtcttagtc tgcgtaaag acaccgcta actcttgta accagcgaa aggaccgat tccgacgtc agcaccgcc ccttccgaa cctgttgaa accgcaaca gcgacagtg gataccgcc attgttggg gacggtttt gttgaacgt gaaattaaa aatttcgct gaggtccaa gggagaaca gatatgact gtccacgaa atccgtctc acaaacccg actccgata aaacaaagg taccgtccc caaaatccg gcaatgcag cagattatc gatgaggaa gttgaccgg atgttaaca gaagacatc atcgaacca tccgagagc ccgtggagt tctccaatt gtcctagcc gacaaaaag gatgggaga aagaagaga ttctgtatc gatttccgt aagatcaat tccgtatcg caaaaggat gcatacccg attccacac attaatggg atcttagac aaattacgt ggggcgaaa tatttttct aaaattgat ttgaaacaa ggatattgg caagttcct ctcgccgaa gaaagtaag cccataaca gctttcact gtgccgaac cggggatta tttcaattc aaagttatg ccatttggt ctccattcc gcaccagca acgttccaa agactatta gaccgggtc ctgggtccg gaaatggac agtggtgca ttcgcctat ttggatgac atcattgtg ctgggacgg accaaagaa gagcaccaa gagaacttg gcaacagtc ttccgtaag ctaagaaag gcaaagctg acaccaaat tgggaaaaa tgctctttt ggtcaaaca aggcttgaa tatctggga catattgtc tcatcacag ggagttagc accgatccc tcgaaagtg aaagccatc ctggaatta ccaccacca actaatgtc aaggaactt cgtcgattt ctaggaata gcctcctgg taccggaag ttcgtgccc gtattttct caagtcgcc gccgcagta aaccgactt ctgaagaag aagcagaaa tgggaatgg ggaaacgaa cagaaggaa gcattcgag aaaatcaaa caactactc accgaagca cccgttctg gcctgcccc gacttcaca aagactttt gttttacag accgacgct agtgaccta ggacttggt gcggccctc atacagaac ctcgatgga ggcgaccgg gtgatcgcc tatgctagt tgtagtctt tcggatcaa caaacgaaa tactcaacg acagagaaa gaatgtttg gcaattgtg tgggcgatc cagaaaatg aggccgtac atcgaggga taccacttc gtcgtagag tccgaccac cagcccctg aagtggctg atgaaacaa ccagaaccc aaaggccgc ctagcgaga tggatcatg acgttacaa caatatgat tttgaggtg cgataccgg aagggagtg ttgaatcaa gtcgccgac gccctatct cgtaacccg atgccgcag aaatccggg atagaggcg attgagact gctcacccc attgacaga tggtaccgg caaaaatat gaagaggta tcagctacc ccagatctc tttccggac tttaagatt gaaaacgac cgattatac aaacacatt atgaacagc cagggactc accggagcg caagaatgg aaattgtgt atcccgaaa ggtcaaaga aagcaagtc ttatttgaa aatcacgac gtcccaacc gctggtcat ttaggaatc gcaaagacg actgcaaga cttgcacaa aaatattat tggcccgga atgtttcga gaagccgca aaatatgtc cagagctgc gaaaactgt caacgttac aagtccagc caacaagcg agacccgga ctaatgcaa ccctacaaa atatacgaa ccgtggcac accgtaagt gtggacttg gtcggccca cttccccgt tcatcaaaa ggcaacagc tacgtactc gtctgccaa gacaggttt acaaaatgg atagaagtt cagcccttg cgtcgggcc accgcacct gtcgtgaca agaacttta tacgaaaat ataattact cgatttggg tgcccgaaa agaatcata accgacaac ggaactcaa ctggagagc cgacttcta aataaactg atgaaagac ttccacata aagcaccga ttaacaccg ccttacaca ccgcaggca aatccagtg gaacgagcg aacaaagtg ttaaagaca atgatagcc caatactgt gaagaaaac caaaggaaa tgggacgaa aatctttgc agtttccgc atggctatc aactcgtca aaacatgag tccactgga ttcacccca gcctatttg aacttaggg cacgaactc cggatgcct tcggcgcta ctacaagaa gacaaaaat gaggaaatc ggatttgac ccggaaaca gaggacgcc gcgatccag gaatacccg aaaaggatt caggagcta caagaacag ttcgaacta gtacgacta aacctttct agggcattc accacgcaa aagaagtac tatgaccgc cgccgtcga gagtggtca tgtcaccca ggtgaccgc gttatgaaa cgagatcac gtgttgtct tcggccgct cgagggttc gctgccaag ctcgctccc aaatattcc ggcccgtac ataatcacc aaagtcctc tctcctgta atggaatca acaccgccg agaaaccgc cactccgag tccatcgag acaaaattg gtgcgcctg tttggacaa tccccggaa agcccctat ctggagcta tatgcatcc ccattgcca ggtctcgtg tcgccgctc ccgccaaca ccgtcccca gtccgcaga agggagatt caggaggcc cttattaag aagctggga acaagcccc atattggca agcccgaca gcctggctg gaatccccg aaaactccg gtgcagttt tccccggaa ccggaaaaa cccgttacc cctggcaaa agcatcgcc caggaggtt ctggacgtt ttccggtcg aagccaagg atactgccg aaaattcca ccggcgccg gaaccgatc aacctggca attcccgac aaccaccgg cggcttccg atgggtcct cctgccacc gagaccgaa gacggcgga caaggcgac gggcgtatc cggcattac gtcatgccg tttctgaag aaagggggg ggtgtaatg aagcatccg gattccgga cgattctat ggtgacgta ggagtcatg cgctcaagg cgaaaccga gcgttctcc agcagttgc tcactgtgc atcaaaccg gtgagtaac tctgggggt cgagccgtg catcaaacc ggcaaaacc ttctccgac gagtggtgc ttgctgcgc atcaaacca gcacaccat ctccaccga cccgaccga cctagagct ccgacgagt ggtgcttgc tgcgcatca aaccagcac accatctcc accgcccag accgacctg gggcttggt agggaaatt acgaccgag gacaaccgc tttccaaaa ggcccaacc gcaacccga tctccgaca aagccgccc ggaaaaagg acattacaa attttcgct gaggtccaa gggagaaca gatatgact gtccacgaa atccgtctc acaaacccg actccgata aaacaaagg taccgaccc caaaatccg gcaatgcag cagattatc gatgaggaa gttgaccgg atgttaaca gaagacatc atcgaacca tccgagagc ccgtggagt tctccaatt gtcctagcc gacaaaaag gatgggaga aagaagaga ttctgtatc gatttccgt aagatcaat tccgtatcg caaaaggat gcatacccg attccacac attaatggg atcttagac aaattacgt ggggcgaaa tatttttct aaaattgat tcgaagcca aggatactg ccgaaaatt ccaccggcg ccggaaccg atcaacctg gcaattccc gacaaccac cggcggctt ccgatgggt cctcctgcc accgagacc gaagacggc ggacaaggc gacgggcgt atccggcat tacgtcatg ccgtttctg aagaaaggg gggggtgta atgaagcat ccggattcc ggacgattc tatggtgac gtaggagtc atgcgctca agacgaaac cgagcgttc tccagcagt tgctcactg tgcatcaaa ccggtgagt aactctggg ggtcgagcc gtgcatcaa accggcaaa accttctcc gacgagtgg tgcttgctg cgcatcaaa ccagcacac catctccac cgacccgac cgacctaga gctccgacg agtggtgct tgctgcgca tcaaaccag cacaccatc tccaccgac ccgaccgac ctggggctt ggctttcca aaaggccca accgcaacc cgatctccg acaaagccg cccggaaaa aggacatta caaattttg gcgcccgag cagggacaa gtcttagtc tgcgtaaag acaccgcta actcttgtg accagcgaa aggaccgat tccgacgtc agcaccgcc ccttccgaa cctgttgaa accgcaaca gcgacagtg gataccgcc attgttggg gacggtttt gttgaacgt gaaattaaa aatttcgct gaggtccaa gggagaaca gatatgact gtccacgaa atccgtctc acaaacccg actccgata aaacaaagg taccgaccc caaaatccg gcaatgcag cagattatc gatgaggaa gttgaccgg atgttaaca gaagacatc atcgaacca tccgagagc ccgtggagt tctccaatt gtcctagcc gacaaaaag gatgggaga aagaagaga ttctgtatc gatttccgt aagatcaat tccgtatcg caaaaggat gcatacccg attccacac attaatggg atcttagac aaattacgt ggggcgaaa tatttttct aaaattgat ttgaaacaa ggatattgg caagttcct ctcgccgaa gaaattttg ctttataca catattgaa gatactcca gttcacaga ttatga
- >TC031778 RA
agagtacaa gatgtctgg caccgacga cgaattgga ttccgacac agaagctgg agacataag agttggatg gatctaccg tttgaggaa acaggaact aatcagtga cttgaagag atttggact ctccaccga aggtaccgt tgaggaact acgacgtcg tctccgaca gtttgtcaa agagaatgc ggccgcagt gggaacaac tccggaaat aaccaacac ggagtctac gatgacggc tttcacgac gacctatgt tccgaacgt attctcctt caacgtacc gccccaagc cggaactgt tcccagagg gtacaccgg aatcaacac ctccacaac ccaggttga aggatggta tacgagacg acctgccac cgaaatgcc acagccgac gttcacgta ccccgctcc tagactatc gtcgttaga aacacaacc agtgattaa tccggtagc tcacctgga ttttgtgaa tcaaatccg gaagtggaa cgttacttt cgatggtaa gaatgatgc agtttcttt cctagaacg tgttgagga actggccga agaatacat gataccaaa gaaccatct agtacgggc attaccgga attactccg gaatcaagc aatcctgtg gtaccggaa tcagaaaag cgacattca ctcttggaa cgatttcgt cagggtttt cagagcatt ttattttcc tcgagagta ccgtgaggg ccttgaaga agaaatctt ccggagggt gcagagacc gcaggagaa tggccgtga cttcgtaat ccagttgca aactctgat ccggcgaca cggaggatg gacaaggga ccaagaact gaattgtat atacaaaca cttgctccc agagtaccg acaatacat acgcaaaaa cgacgtccg ctccgtaat ggaattatt ggaccaaat ccaggaata cgaagggtt gactcagga aataagacg gtcgaaatc agacgtgaa gagctgcat gagggactg cacccgaaa aggctccga ccagtcccc ggaactagg agccattac cggcacacc gtacccgat tggacaagt tttccccga ttttgcgaa taatagccg agtcttgtg gaaaattgt ggtagaaaa ccaagaaat tccggccct gattgacac cgggtccgt gctctccta catcaacga agagacggc caaacattg tataagtca aggactgca gccacaatt tttatcctc caggatgca agccaagat ggcaaatgg ggtaaccgt cgatacaaa cttgttctt taacatcaa attccaggc aagtgactt agaattttc ctgtgattt tctgctctg tccgaactt aactagtcc aatgatctt ggggttgga ctttatcat ggagcaaca gctagtatt aaattttga aaccggaga aatttctcg acagggaca agtcttagt ctgcgtaaa gacaccgct aactcttgt gaccagcga aaggaccga ttccgacgt cagcaccgc cccttccga acctgttga aaccgcaac agcgacagt ggataccgc cattgttgg ggacggttt tgttgaacg tgaaattaa aaatttcgc tgaggtcca agggagaac agatatgac tgtccacga aatccgtct cacaaaccc gactccgat aaaacaaag gtaccgacc ccaaaatcc ggcaatgca gcagattat cgatgagga agttgaccg gatgttaac agaagacat catcgaacc atccgagag cccgtggag ttctccaat tgtcctagc cgacaaaaa ggatgggag aaagaagag attctgtat cgatttccg taagatcaa ttccgtatc gcaaaagga tgcataccc gattccaca cattaatgg gatcttaga caaattacg tggggcgaa atatttttc taaaattga ttcgaagcc aaggatact gccgaaaat tccaccggc gccggaacc gatcaacct ggcaattcc cgacaacca ccggcggct tccgatggg tcctcctgc caccgagac cgaagacgg cggacaagg cgacgggcg tatccggca ttacgtcat gccgtttct gaagaaagg ggggggtgt aatgaagca tccggattc cggacgatt ctatggtga cgtaggagt catgcgctc aagtcagtt ccaccacca ccggcgctc ggtttgttt acaccgtgt cgccaataa aagggcgaa accgagcgt tctccagca gttgctcac tgtgcatca aaccggctc tccaaaagg cccaaccgc aacccgatc tccgacaaa gccgcccgg aaaaaggac attacaaat tttggcgcc cgagcaggg acctgaagg gtctgaagg aaaagaagc tggagacat aagagttgg atggatcta ccgtttgag gaaacagga actaatcag tgacttgaa gagatttgg actctccac cgaaggtac tgttgagga actacgacg tcgtctccg acagtttgt caaagagaa tgcggccgc agttggaac aactccgga aataaccaa cacggagtg tacgatgac ggctttcac gacgaccta tgttccgaa cgtattctc cttcaacgt accgcccca agccggaac tgttcccag agggtacac cggaatcaa cacctccac aacccaggt ggaaggatg gtatacgag acgacctgc caccgaaat gccacagcc gacgttcac gtaccccgc tcctagact atcgtcgtt agaaacaca accagtgat taatccggt agctcacct ggattttgt gaatcaaat ccggaagtg gaacgttac tttcgatgg taagaatga tgcagtttc tttcttaga acgtgttga ggaactggc cgaagaata catgatacc gaagaacca tctagtacg ggcattacc ggaattact ccggaatca agcaatcct gtggtaccg gaatcagaa aagcgacat tcactcttg gaacgattt cgtcagggt tttcagagc attttattt tcctcgaga gtaccgtga gggccttga agaagaaat cttccggag ggtgcagag accgcagga gaatggccg tgacttcgt aatccagtt gcaaactct gatccggcg acacggagg atggacaag ggaccaaga actgaattg tatatacaa acacttgct cccagagta ccgacaata catacgcaa gaacgacgt ccgctccgt aatggaatt attggacca aatccagga atacgaagg gttgactca ggaaataag aaggtcgaa atcagacgt gaagagctg catgaggga ctgcacccg aaaaggctc cgaccagtc cccggaact aggagccat taccggcac accgtaccc gattggaca agttttccc cgattttgc gaataatag ccgagtctt gtggaaaat tgtggtaga aaaccaaga aattccggc cctgattga caccgggtc cgtgctctc ctacatcaa cgaagagac ggccaaaca ttgtataag tcaaggact gcagccaca atttttatc ctccaggat gcaagccaa gatggcaaa tggggtaac cgtcgatac aaacttgtt ctttaacat caaattcca ggcaagtga cttagaatt ttcctgtga ttttctgct ctgtccgaa cttaactag tccaatgat cttggggtt ggactttat catggagca acagctaat attaaattt tgaaaccgg agaaatttc tcgacaggg acaagtctt agtctgcgt aaagacacc gctaactct tgtgaccag cgaaaggac cgattccga cgtcagcac cgccccttc cgaacctgt tgaaaccgc aacagcgac agtggatac cgccattgt tggggacgg ttttgttga acgtgaaat taaaaattt cgctgaggt ccaagggag aacagatat gactgtcca cgaaatccg tctcacaaa cccgactcc gataaaaca aaggtaccg accccaaaa tccggcaat gcagcagat tatcgatga ggaagttga ccggatgtt aacagaagg catcatcga accatccga gagcccgtg gagttctcc aattgtcct agccgacaa aaaggatgg gagaaagaa gagattctg tatcgattt ccgtaagat caattccgt atcgcaaaa ggatgcata cccgattcc acacattaa tgggatctt agacaaatt acgtggggc gaaatattt ttctaaaat tgatttgaa acaaggata ttggcaagt tcctctcgc cgaagaaag taagcccat aacagcttt caccgtgcc gaaccgggg attatttca attcaaagt tatgccatt tggtctcca ttccgcacc agcaacgtt ccaaagact attagaccg ggtcctggg tccggaaat ggacagtgg tgcattcgc ctatttgga tgacatcat tgtgctggg acggaccaa agaagagca ccaagagaa cttggcaac agtcttccg taagctaag aaaggcaaa gctgacacc aaattggga aaaatgctc ttttggtca aacaaggct tgaatatct gggacatat tgtctcatc acagggagt tagcaccga tccctcgaa agtgaaagc catcctgga attaccacc accaactaa tgtcaagga acttcgtcg atttctagg aatagcctc ctggtaccg gaagttcgt gcccgtatt ttctcaagt cgccgccgc attaaaccg acttctgaa gaagaagca gaaatggga atggggaaa cgaacagaa ggaagcatt caagaaaat caaacaact actcaccga agcacccgt tctggcctg ccccgactt cacaaagac ttttgtttt acagaccga cgctagtga cctaggact tggtgcggc cctcataca gaacctcga tggaggcga ccgggtgat cgcctatgc tagttgtag tctttcgga tcaacaaac gaaatactc aacgacaga gaaagaatg tttggcaat tgtgtgggc gatccagaa aatgaggcc gtacatcga gggatacca cttcgtcgt agagtccga ccaccagcc cctgaagtg gctgatgaa acaaccaga acccaaagg ccgcctagc gagatggat catgacgtt acaacaata tgattttga ggtgcgata ccggaaggg agtgttgaa tcaagtcgc cgacgccct atctcgtaa cccgatgcc gcagaaatc cgggataga ggcgattga gactgctca ccccatcga cggatggta ccggcaaaa atatgaaga ggtatcagc taccccaga tctctttcc ggactttaa gattgaaaa cgaccgatt atacaaaca cattatgaa cagccaggg actcaccgg agcgcaaga atggaaatt gtgtatccc gaaaggtca aagaaagca agtcttgtt tgaaaatca cgacgtccc aaccgctgg tcatttagg aatcgcaaa gacaactgc aagacttgc acaaaaata ttattggcc cggaatgtt tcgagaagc cgcaaaata tgtccagag ctgtgaaaa ctgtcaacg ttacaagtc cagccaaca agcgagacc cggactaat gcaacccta caaaatata cgaaccgtg gcacaccgt aagtgtgga cttggtcgg cccacttcc ccgttcatc aaaaggcaa cagctacgt actcgtctg ccaagacag gtttacaaa atggataga agttcagcc cttgcgtcg ggccaccgc acctgtcgt gacaagaac tttatacga aaatataat tactcgatt tgggtgccc gaaaagaat cataaccga caacggaac tcaactgga gagccgact tctaaataa actgatgaa agacttcca cttaaagca ccgattaac accgcctta cacaccgca ggcaaatcc agtggaacg agcgaacaa agtgttaaa gacaatgat agcccaata ctgtgaaga aaaccaaag gaaatggga cgaaaatct ttgcagttt ccgcatggc tatcaactc gtcaaaaca tgagtccac tggattcac cccagccta tttgaactt agggcacga actccggat gccttcggc gctactaca agaagacaa aaatgagga aatcggatt tgacccgga aacagagga cgccgcgat ccaggaata cccgaaaag gattcagga gctacaaga acagttcga actagtacg actaaacct ttctagggc attcaccac gcaaaagaa gtactatga ccgccgccg tcgagagtg gtcatgtca cccaggtga ccgcgttat gaaacgaga tcacgtgtt gtcttcggc cgctcgagg gttcgctgc caagctcgc tcccaaata ttccggccc gtacataat caccaaagt cctctctcc tgtaatgga atcaacacc gccgagaaa ccgccactc cgagtccat cgagacaaa attggtgcg cctgtttgg acaatcccc ggaaagccc ctatctgga gctatatgc atccccatt accaggtct cgtgtcgcc gctcccgcc aacaccgtc cccagtccg cagaaggga gattcagga ggcccttat taagaagct gggaacaag ccccatatt ggcaagccc gacagcctg gctggaatc cccgaaaac tccggtgca gttttcccc ggaaccgga aaaacccgt tacccctgg caaaagcat cgcccagga ggttctgga cgttttccg gtcgaagcc aaggatact gccgaaaat tccaccggc gccggaacc gatcaacct ggcagtccc actttggac tttccggcc agcgggtaa tacagaggg gcgatgccg attcagatt cccgacaac caccggcgg cttccgatg ggtcctcct gccaccgag accgaagac ggcggacaa ggcgacggg cgagtcatg cgctcaagt cagttccac caccaccgg cgctcggtt tgtttacac cgtgtcgcc aataaaagg gcgaaaccg agcgttctc cagcagttg ctcactgtg catcaaacc ggtgagtaa ctctggggg tcgagccgt gcatcaaac cggcaaaac cttctccga caagtggtg cttgctgcg catcaaacc agcacacca tctccaccg acccgaccg acctagagc tccgacgag tggtgcttg ctgcgcatc aaaccagca caccatctc caccgccca gaccgacct ggggcttgg tagggaaat tacgaccga ggacaacca gagagagta caagatgtc tggcaccga cgacgaatt ggattccga cacagaagc tggagacat aagagttgg atggatcta ccgtttgag gaaacagga actaatcag tgacttgaa gagatttgg actctccac cgaaggtac tgttgagga actacgacg tcgtctccg acagtttgt caaagagaa tgcggccgc agttggaac aactccgga aataaccaa cacggagtc tacgatgac ggctttcac gacgaccta tgttccgaa cgtattctc cttcaacgt accgcccca agccggaac tgttcccag agggtacac cggaatcaa cacctccac aacccaggt tgaaggatg gtatacgag acgacctgc caccgaaat gccacagcc gacgttcac gtaccccgc tcctagact atcgtcgtt agaaacaca accagtgat taatccggt agctcacct ggattttgt gaatcaaat ccggaagtg gaacgttac tttcgatgg taagaatga tgcagtttc tttcctaga acgtgttga ggaactggc cgaagaata catgatacc gaagaacca tctagtacg ggcattacc ggaattact ccggaatca agcaatcct gtggtaccg gaatcagaa aagcgacat tcactcttg gaacgattt cgtcagggt tttcagagc attttattt tcctcgaga gtaccgtga gggccttga agaagaaat cttccggag ggtgcagag accgcagga gaatggccg tgacttcgt aatccagtt gcaaactct gatccggcg acacggagg atggacaag ggaccaaga actgaattg tatatacaa acacttgct cccagagta ccgacaata catacgcaa aaacgacgt ccgctccgt aatggaatt attggacca aatccagga atacgaagg gttgactca ggaaataag acggtcgaa atcagacgt gaagagctg catgagtac cacatcaaa ttggacaaa ggcaaagcc cggattttc gatgagcac tccgcaaca aacgtcatt acaaagacc aaaaccggc accaagacc cacatggag ctcgaacag caacaaaca gtctgattt agaaaggtt atgttggag atgcggtca aaagggcca cacacgcct acaatgccg acaaccagc aatactgtt ctgctcaag atgtggacg ccaaggggt aaaatcgat cgagtgtcc ctgcccctt taaaccggg aaattcaaa ggggactgc acccgaaaa aggctccga ccagtcccc ggaactagg agccattac cggcacacc gtacccgat tggacaagt tttccccga ttttgcgaa taatagccg agtcttgtg gaaaattgt ggtagaaaa ccaagaaat tccggccct gattgacac cgggtccgt gctctccta catcaacga agagacggc caaacattg tataagtca aggactgca gccacaatt tttatcctc caggatgca agccaagat ggcaaatgg ggtaaccgt cgatacaaa cttgttctt taacatcaa attccaggc aagtgactt agaattttc ctgtgattt tctgctctg tccgaactt aactagtcc aatgatctt ggggttgga ctttatcat ggagcaaca gctagtatt aaattttga aaccggaga aatttctcg acagggaca agtcttagt ctgcgtaaa gacaccgct aactcttgt aaccagcga aaggaccga ttccgacgt cagcaccgc cccttccga acctgttga aaccgcaac agcgacagt ggataccgc cattgttgg ggacggttt tgttgaacg tgaaattaa aaatttcgc tgaggtcca agggagaac agatatgac tgtccacga aatccgtct cacaaaccc gactccgat aaaacaaag gtaccgtcc ccaaaatcc ggcaatgca gcagattat cgatgagga agttgaccg gatgttaac agaagacat catcgaacc atccgagag cccgtggag ttctccaat tgtcctagc cgacaaaaa ggatgggag aaagaagag attctgtat cgatttccg taagatcaa ttccgtatc gcaaaagga tgcataccc gattccaca cattaatgg gatcttaga caaattacg tggggcgaa atatttttc taaaattga tttgaaaca aggatattg gcaagttcc tctcgccga agaaagtaa gcccataac agctttcac tgtgccgaa ccggggatt atttcaatt caaagttat gccatttgg tctccattc cgcaccagc aacgttcca aagactatt agaccgggt cctgggtcc ggaaatgga cagtggtgc attcgccta tttggatga catcattgt gctgggacg gaccaaaga agagcacca agagaactt ggcaacagt cttccgtaa gctaagaaa ggcaaagct gacaccaaa ttgggaaaa atgctcttt tggtcaaac aaggcttga atatctggg acatattgt ctcatcaca gggagttag caccgatcc ctcgaaagt gaaagccat cctggaatt accaccacc aactaatgt caaggaact tcgtcgatt tctaggaat agcctcctg gtaccggaa gttcgtgcc cgtattttc tcaagtcgc cgccgcagt aaaccgact tctgaagaa gaagcagaa atgggaatg gggaaacga acagaagga agcattcga gaaaatcaa acaactact caccgaagc acccgttct ggcctgccc cgacttcac aaagacttt tgttttaca gaccgacgc tagtgacct aggacttgg tgcggccct catacagaa cctcgatgg aggcgaccg ggtgatcgc ctatgctag ttgtagtct ttcggatca acaaacgaa atactcaac gacagagaa agaatgttt ggcaattgt gtgggcgat ccagaaaat gaggccgta catcgaggg ataccactt cgtcgtaga gtccgacca ccagcccct gaagtggct gatgaaaca accagaacc caaaggccg cctagcgag atggatcat gacgttaca acaatatga ttttgaggt gcgataccg gaagggagt gttgaatca agtcgccga cgccctatc tcgtaaccc gatgccgca gaaatccgg gatagaggc gattgagac tgctcaccc cattgacag atggtaccg gcaaaaata tgaagaggt atcagctac cccagatct ctttccgga ctttaagat tgaaaacga ccgattata caaacacat tatgaacag ccagggact caccggagc gcaagaatg gaaattgtg tatcccgaa aggtcaaag aaagcaagt cttatttga aaatcacga cgtcccaac cgctggtca tttaggaat cgcaaagac gactgcaag acttgcaca aaaatatta ttggcccgg aatgtttcg agaagccgc aaaatatgt ccagagctg cgaaaactg tcaacgtta caagtccag ccaacaagc gagacccgg actaatgca accctacaa aatatacga accgtggca caccgtaag tgtggactt ggtcggccc acttccccg ttcatcaaa aggcaacag ctacgtact cgtctgcca agacaggtt tacaaaatg gatagaagt tcagccctt gcgtcgggc caccgcacc tgtcgtgac aagaacttt atacgaaaa tataattac tcgatttgg gtgcccgaa aagaatcat aaccgacaa cggaactca actggagag ccgacttct aaataaact gatgaaaga cttccacat aaagcaccg attaacacc gccttacac accgcaggc aaatccagt ggaacgagc gaacaaagt gttaaagac aatgatagc ccaatactg tgaagaaaa ccaaaggaa atgggacga aaatctttg cagtttccg catggctat caactcgtc aaaacatga gtccactgg attcacccc agcctattt gaacttagg gcacgaact ccggatgcc ttcggcgct actacaaga agacaaaaa tgaggaaat cggatttga cccggaaac agaggacgc cgcgatcca ggaataccc gaaaaggat tcaggagct acaagaaca gttcgaact agtacgact aaacctttc tagggcatt caccacgca aaagaagta ctatgaccg ccgccgtcg agagtggtc atgtcaccc aggtgaccg cgttatgaa acgagatca cgtgttgtc ttcggccgc tcgagggtt cgctgccaa gctcgctcc caaatattc cggcccgta cataatcac caaagtcct ctctcctgt aatggaatc aacaccgcc gagaaaccg ccactccga gtccatcga gacaaaatt ggtgcgcct gtttggaca atccccgga aagccccta tctggagct atatgcatc cccattgcc aggtctcgt gtcgccgct cccgccaac accgtcccc agtccgcag aagggagat tcaggaggc ccttattaa gaagctggg aacaagccc catattggc aagcccgac agcctggct ggaatcccc gaaaactcc ggtgcagtt ttccccgga accggaaaa acccgttac ccctggcaa aagcatcgc ccaggaggt tctggacgt tttccggtc gaagccaag gatactgcc gaaaattcc accggcgcc ggaaccgat caacctggc aattcccga caaccaccg gcggcttcc gatgggtcc tcctgccac cgagaccga agacggcgg acaaggcga cgggcgtat ccggcatta cgtcatgcc gtttctgaa gaaaggggg gggtgtaat gaagcatcc ggattccgg acgattcta tggtgacgt aggagtcat gcgctcaag gcgaaaccg agcgttctc cagcagttg ctcactgtg catcaaacc ggtgagtaa ctctggggg tcgagccgt gcatcaaac cggcaaaac cttctccga cgagtggtg cttgctgcg catcaaacc agcacacca tctccaccg acccgaccg acctagagc tccgacgag tggtgcttg ctgcgcatc aaaccagca caccatctc caccgccca gaccgacct ggggcttgg tagggaaat tacgaccga ggacaaccg ctttccaaa aggcccaac cgcaacccg atctccgac aaagccgcc cggaaaaag gacattaca aattttcgc tgaggtcca agggagaac agatatgac tgtccacga aatccgtct cacaaaccc gactccgat aaaacaaag gtaccgacc ccaaaatcc ggcaatgca gcagattat cgatgagga agttgaccg gatgttaac agaagacat catcgaacc atccgagag cccgtggag ttctccaat tgtcctagc cgacaaaaa ggatgggag aaagaagag attctgtat cgatttccg taagatcaa ttccgtatc gcaaaagga tgcataccc gattccaca cattaatgg gatcttaga caaattacg tggggcgaa atatttttc taaaattga ttcgaagcc aaggatact gccgaaaat tccaccggc gccggaacc gatcaacct ggcaattcc cgacaacca ccggcggct tccgatggg tcctcctgc caccgagac cgaagacgg cggacaagg cgacgggcg tatccggca ttacgtcat gccgtttct gaagaaagg ggggggtgt aatgaagca tccggattc cggacgatt ctatggtga cgtaggagt catgcgctc aagacgaaa ccgagcgtt ctccagcag ttgctcact gtgcatcaa accggtgag taactctgg gggtcgagc cgtgcatca aaccggcaa aaccttctc cgacgagtg gtgcttgct gcgcatcaa accagcaca ccatctcca ccgacccga ccgacctag agctccgac gagtggtgc ttgctgcgc atcaaacca gcacaccat ctccaccga cccgaccga cctggggct tggctttcc aaaaggccc aaccgcaac ccgatctcc gacaaagcc gcccggaaa aaggacatt acaaatttt ggcgcccga gcagggaca agtcttagt ctgcgtaaa gacaccgct aactcttgt gaccagcga aaggaccga ttccgacgt cagcaccgc cccttccga acctgttga aaccgcaac agcgacagt ggataccgc cattgttgg ggacggttt tgttgaacg tgaaattaa aaatttcgc tgaggtcca agggagaac agatatgac tgtccacga aatccgtct cacaaaccc gactccgat aaaacaaag gtaccgacc ccaaaatcc ggcaatgca gcagattat cgatgagga agttgaccg gatgttaac agaagacat catcgaacc atccgagag cccgtggag ttctccaat tgtcctagc cgacaaaaa ggatgggag aaagaagag attctgtat cgatttccg taagatcaa ttccgtatc gcaaaagga tgcataccc gattccaca cattaatgg gatcttaga caaattacg tggggcgaa atatttttc taaaattga tttgaaaca aggatattg gcaagttcc tctcgccga agaaatttt gctttatac acatattga agatactcc agttcacag attatgatg gaatttaga ctcaccccg tatgtttcc gaataacaa aattgtgta tgcaaaacg aaatcaaaa tttccccga cagtcgtaa tgcaactct ataaacaaa aaaataatc aatcactta cctagaaaa ccgtttgat tgttgaagg ttatgaacc ggaaccaag tattgaagt aacatcgac acaaactct taacacaca ttgcactaa ttaccaaat caatacctt catttgcat gaaattaac aagacacgc cagcaaaac aatgcgaca acaaaacca agacattgc cagacatga ggaacaaac gtctattcg aacataacc atacttttc aatgaagca
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