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Gene information
General information
TC006854 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 10881..47079
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006854
    iBeetle screen iB_04228

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006854 RA
      MQPPPRKGN YAKFLKNLH TEQINKLAL KNQHECDLL 
      EDIRSFTIR KSALEKTHS EDLLKLTSQ FLNKKAPNI 
      PDIKIEGSE EKWNMWNVW RTVLEENEK LARARLAAV 
      EVFQQQIAD DAKILRVHK LQAAKKCLD QLALVQKEL 
      QGCVQEVDK TKKMYFDEE HSAHGVRDK ARDIEEKLK 
      KKKGGFFTS ITSLQKNSA KYSTKREQL DEKSTGARN 
      DYLLCLAAA NAHQNRYFV VDLQQCMST MESGVYEKV 
      AEYLFLMGR TELLTCSAM QNSFTIIRD QAKQLTRDY 
      NIQCCYLYY PVLKQHIQY PFEPCDNDP IETITAEHD 
      SAAATLSKE ARRWATRIA RENNVIRES TRKLQIYSA 
      LRESGQKVD PNDQNGPDL ETKIEEMRQ NIRKAETAK 
      AKAEARIEA LREGGVNVD EWLQEAESL NVQDIQRSA 
      SSLSDHPSS DSFYDSDFA EGETATATA PQADKHQDK 
      DSEGSQHDQ FENAEVDAM LEQERQRIE QLTAGWDDP 
      TQVDWGTED TESESVIRR ESTPSMPLL KCTALYSYT 
      AQNPDELTI VENEQLEVV GEGDGDGWL RARNYKGEE 
      GYVPHNYLD VEREQTATT PGLVNQISF SSVDYTVDN 
      EEEVQAPSE TNQSPEQVS VISVQAAVP DSPAASYCI 
      ALYDYDGEG GEELTFEEG QIIKVLSKC AHSVDDGWW 
      KGELEGRIG NFPSLVVEE CDEYGEPLT NEWDETPPP 
      SAPPVFTPP DVPNFLVAA EVVITQPTP SVEHPQEPP 
      PVLKNQGDS TASPEGSFS MELSRDQHQ QYGSQFQGN 
      VPTIDVPSV EIVGEDDQP GPESDPQQD ELGLGVAQI 
      VITAATPMM EEPEHPFPP PEEPAANHR ETDTAAAAA 
      AIPEEDEEE VDAEPAPGA ADGDSTPFV VSSSTGSDG 
      ETTGPSTAE NSVSHVVGE PKQVVGGRA SIPDELEPH 
      QLARLQDLK ESNA
    CDS sequence
    • >TC006854 RA
      atgcagccg ccgccgaga aagggtaat tacgccaaa 
      ttcctcaaa aacctccac acggagcaa atcaacaaa 
      ctcgcgctc aagaaccag cacgagtgt gacctgctc 
      gaggacatt aggagcttt acgatcagg aaatcagcg 
      ttagagaaa acgcactct gaagacttg cttaagctg 
      acctcgcag ttcctcaat aagaaggca cccaatatt 
      cccgatatc aaaatcgaa ggtagtgag gaaaaatgg 
      aacatgtgg aacgtttgg cgaacggtt ctggaagag 
      aacgaaaaa ctggcaagg gctcgatta gcagccgtc 
      gaagttttt cagcagcag atagcagac gacgcgaaa 
      attttaagg gtgcataag ctccaagca gcgaaaaaa 
      tgtttggat cagttggct ctcgtacaa aaggagtta 
      caaggatgt gtacaagaa gtggacaag acgaaaaaa 
      atgtacttt gatgaggaa cacagtgct catggggtc 
      agggacaag gcgagagac atcgaagaa aaactcaaa 
      aagaagaag ggcggtttc ttcacctca atcacctcc 
      ttgcagaaa aacagtgca aaatactca acaaaacgc 
      gaacaactc gacgaaaaa tcgacgggc gcccgcaac 
      gactacctc ctgtgcctg gcggctgca aacgcccat 
      caaaatcgc tactttgtc gtcgattta caacaatgt 
      atgtcgacg atggaaagc ggcgtctac gaaaaagtc 
      gccgaatat ctttttctg atgggacgc actgaactt 
      ctcacctgc tcggcgatg caaaactcc ttcacaatc 
      atccgagat caagccaaa caactaacc agagattat 
      aatattcaa tgctgttac ttgtactat cccgttctt 
      aaacagcac atacaatat cctttcgaa ccgtgtgac 
      aacgatcct attgaaacc atcacagca gaacatgat 
      tcagcggcc gctaccttg agcaaagag gccagaagg 
      tgggccacg aggatagca agggagaat aacgtgatc 
      agggagagc accaggaag ttgcagatt tatagcgcg 
      ctcagggag tcggggcaa aaggtggat ccaaacgac 
      cagaacggc cccgatctc gaaacgaaa atcgaagag 
      atgaggcag aatattcgg aaagcggaa acggcgaaa 
      gccaaagcc gaagctcgg atagaggcg ctcagggag 
      ggcggcgtc aacgtcgac gagtggctg caggaggcc 
      gagtcgctc aatgtccag gatatccaa cgatcggca 
      agctcatta tcggaccac cccagctcg gactcgttc 
      tacgacagc gactttgcc gaaggcgag actgccacc 
      gccaccgct ccccaggcc gacaagcac caggataaa 
      gacagcgaa gggagtcaa cacgatcag tttgaaaat 
      gcagaggtc gacgcgatg ctcgaacag gagagacaa 
      aggatcgaa cagttgact gcaggttgg gacgacccc 
      acacaagtc gattggggt acggaggat acggaatcg 
      gagagtgtg attcgacga gagtcgacc ccttccatg 
      cctctgctc aagtgcaca gcgctctac tcgtacact 
      gcacaaaat cccgacgag ttgacgatc gtcgaaaac 
      gaacaactg gaagtggta ggtgaaggt gacggtgat 
      gggtggttg cgagcgcgc aattacaaa ggtgaggaa 
      ggatacgta ccgcataat tacctcgac gtggagcgc 
      gaacagacg gcgacgacg cccggacta gtcaatcaa 
      atatcattt tcgtcggtc gactacacc gtagacaac 
      gaggaggag gtgcaagca ccgtccgaa acgaatcaa 
      tcgcccgaa caagtctcc gttatttca gtgcaagcc 
      gcagttccc gattcacct gcagcgtcc tattgtatc 
      gccctctac gattacgac ggcgaaggt ggcgaagaa 
      cttactttc gaagaggga caaataatt aaagttttg 
      agcaaatgt gcgcatagt gttgacgac ggctggtgg 
      aaaggcgaa ctggaaggt agaatcgga aactttccg 
      tcgttggtg gtggaggag tgtgacgaa tatggcgaa 
      ccgttgacg aatgaatgg gacgaaacg ccgccaccg 
      tcagcacca ccggttttt acaccacct gatgttccc 
      aatttttta gtagcagct gaggtggtt ataacgcaa 
      ccaacgcca tctgtagaa cacccacaa gaaccacct 
      ccagttctc aaaaatcaa ggagattca acagcatca 
      ccagaggga agcttctcc atggagcta tcccgggac 
      caacaccaa caatacggc agtcaattt cagggaaat 
      gttccaacg atcgacgtt ccgagcgtc gagatcgtt 
      ggcgaggat gaccagccc ggccccgag tccgacccc 
      cagcaggac gagctgggt ctcggcgtg gctcagatc 
      gtgataacg gccgccacc cccatgatg gaggaaccc 
      gagcacccg ttcccgccg ccggaggag ccggccgcc 
      aaccaccgc gagacggac accgccgcc gccgccgcc 
      gccatcccc gaggaggac gaggaggag gtggacgcc 
      gagccggcg ccgggcgcc gccgacggc gactcgacg 
      ccgttcgtc gtcagcagc agcacgggc agcgacggc 
      gagacgacg ggccccagc accgccgag aactcggtg 
      agtcacgtg gtgggcgag cccaagcag gtggtgggc 
      ggcagggcg agcatcccc gacgagctg gagccgcac 
      cagctggcc cgactgcaa gatctcaaa gagtccaac 
      gcctag
    mRNA sequence
    • >TC006854 RA
      atctaataa aaaatagat gatatatgg aaataatgc 
      cccgtctgt tcaaaattt cttctgcgt cccatcgaa 
      ctgcaataa tcgcgccgc ctccaagcc aagtcaaat 
      tttccgtct gtattttat ctcgctaat ctgtcagcc 
      aagtgtcaa caagcactc cagtacccg tgtgttatc 
      acctcgttg gaattaaaa gccgctgat cggagaata 
      attcgcatt cgatcagcg atgtctctt ctcattgtg 
      gggggcgtt tgcacggcg ccgccgcca gctctgtcg 
      gtcagtggg tggcataat caatagacg tcgtagtcg 
      gaagtgtcg ggagcggtt gtgtgtgcg gagtgaatg 
      ccaggcata gatttttct ccgaggaga ccatgcagc 
      cgccgccga gaaagggta attacgcca aattcctca 
      aaaacctcc acacggagc aaatcaaca aactcgcgc 
      tcaagaacc agcacgagt gtgacctgc tcgaggaca 
      ttaggagct ttacgatca ggaaatcag cgttagaga 
      aaacgcact ctgaagact tgcttaagc tgacctcgc 
      agttcctca ataagaagg cacccaata ttcccgata 
      tcaaaatcg aaggtagtg aggaaaaat ggaacatgt 
      ggaacgttt ggcgaacgg ttctggaag agaacgaaa 
      aactggcaa gggctcgat tagcagccg tcgaagttt 
      ttcagcagc agatagcag acgacgcga aaattttaa 
      gggtgcata agctccaag cagcgaaaa aatgtttgg 
      atcagttgg ctctcgtac aaaaggagt tacaaggat 
      gtgtacaag aagtggaca agacgaaaa aaatgtact 
      ttgatgagg aacacagtg ctcatgggg tcagggaca 
      aggcgagag acatcgaag aaaaactca aaaagaaga 
      agggcggtt tcttcacct caatcacct ccttgcaga 
      aaaacagtg caaaatact caacaaaac gcgaacaac 
      tcgacgaaa aatcgacgg gcgcccgca acgactacc 
      tcctgtgcc tggcggctg caaacgccc atcaaaatc 
      gctactttg tcgtcgatt tacaacaat gtatgtcga 
      cgatggaaa gcggcgtct acgaaaaag tcgccgaat 
      atctttttc tgatgggac gcactgaac ttctcacct 
      gctcggcga tgcaaaact ccttcacaa tcatccgag 
      atcaagcca aacaactaa ccagagatt ataatattc 
      aatgctgtt acttgtact atcccgttc ttaaacagc 
      acatacaat atcctttcg aaccgtgtg acaacgatc 
      ctattgaaa ccatcacag cagaacatg attcagcgg 
      ccgctacct tgagcaaag aggccagaa ggtgggcca 
      cgaggatag caagggaga ataacgtga tcagggaga 
      gcaccagga agttgcaga tttatagcg cgctcaggg 
      agtcggggc aaaaggtgg atccaaacg accagaacg 
      gccccgatc tcgaaacga aaatcgaag agatgaggc 
      agaatattc ggaaagcgg aaacggcga aagccaaag 
      ccgaagctc ggatagagg cgctcaggg agggcggcg 
      tcaacgtcg acgagtggc tgcaggagg ccgagtcgc 
      tcaatgtcc aggatatcc aacgatcgg caagctcat 
      tatcggacc accccagct cggactcgt tctacgaca 
      gcgactttg ccgaaggcg agactgcca ccgccaccg 
      ctccccagg ccgacaagc accaggata aagacagcg 
      aagggagtc aacacgatc agtttgaaa atgcagagg 
      tcgacgcga tgctcgaac aggagagac aaaggatcg 
      aacagttga ctgcaggtt gggacgacc ccacacaag 
      tcgattggg gtacggagg atacggaat cggagagtg 
      tgattcgac gagagtcga ccccttcca tgcctctgc 
      tcaagtgca cagcgctct actcgtaca ctgcacaaa 
      atcccgacg agttgacga tcgtcgaaa acgaacaac 
      tggaagtgg taggtgaag gtgacggtg atgggtggt 
      tgcgagcgc gcaattaca aaggtgagg aaggatacg 
      taccgcata attacctcg acgtggagc gcgaacaga 
      cggcgacga cgcccggac tagtcaatc aaatatcat 
      tttcgtcgg tcgactaca ccgtagaca acgaggagg 
      aggtgcaag caccgtccg aaacgaatc aatcgcccg 
      aacaagtct ccgttattt cagtgcaag ccgcagttc 
      ccgattcac ctgcagcgt cctattgta tcgccctct 
      acgattacg acggcgaag gtggcgaag aacttactt 
      tcgaagagg gacaaataa ttaaagttt tgagcaaat 
      gtgcgcata gtgttgacg acggctggt ggaaaggcg 
      aactggaag gtagaatcg gaaactttc cgtcgttgg 
      tggtggagg agtgtgacg aatatggcg aaccgttga 
      cgaatgaat gggacgaaa cgccgccac cgtcagcac 
      caccggttt ttacaccac ctgatgttc ccaattttt 
      tagtagcag ctgaggtgg ttataacgc aaccaacgc 
      catctgtag aacacccac aagaaccac ctccagttc 
      tcaaaaatc aaggagatt caacagcat caccagagg 
      gaagcttct ccatggagc tatcccggg accaacacc 
      aacaatacg gcagtcaat ttcagggaa atgttccaa 
      cgatcgacg ttccgagcg tcgagatcg ttggcgagg 
      atgaccagc ccggccccg agtccgacc cccagcagg 
      acgagctgg gtctcggcg tggctcaga tcgtgataa 
      cggccgcca cccccatga tggaggaac ccgagcacc 
      cgttcccgc cgccggagg agccggccg ccaaccacc 
      gcgagacgg acaccgccg ccgccgccg ccgccatcc 
      ccgaggagg acgaggagg aggtggacg ccgagccgg 
      cgccgggcg ccgccgacg gcgactcga cgccgttcg 
      tcgtcagca gcagcacgg gcagcgacg gcgagacga 
      cgggcccca gcaccgccg agaactcgg tgagtcacg 
      tggtgggcg agcccaagc aggtggtgg gcggcaggg 
      cgagcatcc ccgacgagc tggagccgc accagctgg 
      cccgactgc aagatctca aagagtcca acgcctaga 
      ccatcatgt tagagctct gttcgtacg cttcaaatt 
      aataaataa attaccaaa aaaaaaaat gaaaagggt 
      gattggaaa cgattggaa attgattag gtttgatgg 
      aagatcgtt tcgaatcac ttcactttg taggataaa 
      tacgcatac gtggacgac gcaatggac tatcatcat 
      cttgtgtgt tgttgaatc gcgcgtata tttgtacct 
      aatttgtta ttattgtta gatgaacat atgaggata 
      ttttcactt tagacatgt aagtagttg gtaggttgt 
      cggttgaat tggttacaa ttgttggtt ggacgattt 
      ctttttgcg tttcggtat taatgtcac aaacttaag 
      gtaattttg ttggttttg agtggctcc tgatgtctg 
      ttggaaacg aattttttt gttaattat tttcaggta 
      agtttttta ttatttttc taattgatt aacaatgaa 
      aaaattcta ttgatttca aataaattt cacacacgt 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY