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Gene information
General information
TC006853 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 48378..68165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006853
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01098

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 2
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    dorsal ridge row mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gena triplet seta partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    vertex triplet vertex triplet ventral not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labium position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs pretarsus slightly shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdominal segment 1 cuticle ectopically with protrusion - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 5

    wings not closed - Number of animals showing the phenotype: 5

    wings potentially shortened - Number of animals showing the phenotype: 5
    iBeetle screen iB_04227

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva bristle potentially pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen bristle potentially pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006853 RA
      MSQRAKRPN RSDDGSVPV KRRKRTAAE EEEAASLAV 
      WQPRSNDLK SIYNRTTTE APAELFRKD LISAMKLPD 
      SEPLASNEY WVIVDQWKQ EWERGVQVP VNPDSLPEP 
      SVTVTQNTP LKLQHEFKL PKNKYIRIT KDENFKIEE 
      HALSNAPAQ SEAACSYDL DECDVSWLR ILNSERSQC 
      GQSPINSEQ LERVIEQFE LCCWDKIQS ILRNEEGLG 
      IEYDENVIC DVCRSPDSE EGNEMVFCD SCNICVHQA 
      CYGITRIPE GQWLCCTCH LSKRPKCVL CPNKGGAMK 
      CTRSGQKWA HVSCALWIP EVSIGCVEK MEPITKISS 
      IPQSRWALI CVLCRERVG ACIQCSVKT CKTAYHVTC 
      AFKHGLEMR AIIEDENAD DGVKLRSYC EKHSKSSKN 
      KEKSVCSGS EDDDSRRKK RKDMTSEEK NQARAARLQ 
      EIESEFFKH VSIKDVSIH LDVDNDALH HIYNYWKLK 
      RKAGNNKPL LPPKSEDVD MLSHKREQA DMEKMKMFV 
      QLRQDLERV RNLCYMVSR REKLSRSFF RLREQTFYK 
      QTAVLEAAN NLPNVVVQA VIEANHGPS IYDRLYSHE 
      DAEDHTSDF ERILTRIAG VKSPTDSGD EKRTELNGL 
      FKDKNPYKK IYFNGSSNR RSGSLYGSS SSSDEKDRL 
      NAATTSEDE DKSKKKSSS SVRKPNNNN NKASDRRRK 
      RHSASSKIE SSSEEDSKQ KTKKTWGQF SRLKQVERE 
      LGYSGSDSD ELMPIRASQ KSDTKQMSS IYSDSDSSD 
      VSMTKASDS QQNKLRTKA AVKEFSHKP STSNKTSPE 
      NKKNKKKES PTKKKDYDP SDLIVPQRQ AAKKATENL 
      KSTTTRVVK EQMDEPSVK VEEKPAKLP KPKAKTKET 
      VKEVKEVKK ESAVKTSDV FDLDKETDN AEILAYVPQ 
      RQAAKKAAE HIKSGLGKP VAPSSVDEL EKNKLESTV 
      VKKKEAEKQ QPAKVPESK RKSSSNSSS SSSSSSSTS 
      SSTSSSSDS DDEVPPSQQ QAQQPRSES LFSNYSSKE 
      CRETAKRTN ASKDWPFLD KGAKSAAAA ASSSSTDSS 
      DSSRPTSPI ITTTTTTKT TSPDKKSTT TQREEQTRM 
      RGKGRPATT AARGRPRQS SKSGDRVGD ANVRLRNLS 
      DSQRGDAKC RKGKQDEVA EKETKSVNK LQSPVRKTD 
      KKLTESKES SEVSNLEKE ILERKAGKQ GSSKTKSTL 
      DRLFGSPPD KKDEKKPLN KTTKPTKPE RVKSTVKEN 
      KEPEKIASP VKKSPVKSK NIDEIFTQK TVLNENKIE 
      VKEKEKHPT PKREEIEPA PKIEDFKQN NEKLTEPEK 
      KLPHSRSIF SPPHLKDNL LDFDNLDDG FGISKDEEI 
      MKGPLTFNF GNTSLFKED SKEDSARET LNLVEKLRM 
      EMSKKSTSC DVDDGASVS SSTKNECDR TEFAEQVVP 
      NCVAEKNDA KVDDVPPPT VNLEMRVEV TEAKGFADR 
      GQDYRFMSC EADVPLEQN KNIPIQQHH GLSDHGISG 
      QGDERWVPP SEAYTAAPP IDPSNYGSE SQQPLQTPQ 
      HFMQQYSLN KTQPLLNAI PPQIEMRLH DDTLKNPSP 
      TLHDRRISQ TPLNEDCMP SPSPYADIH PQAKWADSE 
      IMPNRRSSS SSAASTSSS CSRRNDDDD LKLRPDVMM 
      VNHQALEIG YLHPFPPEL FPTLSDSSQ FVSPVSLLH 
      PPPLNPSLA FPSTAPAML PPAFAPFPP AQVMAPTLS 
      KPLEDVPHC TAAFTSSQH NMALTAAIA NLPPSLPKE 
      VEALPEDEV VPTTQSNVL SSPSPSIKS NSSGKKSPS 
      KPTRTSARV TSQGKSPKQ DVVKPSGRG RGEAKRNSV 
      KSNAARGRG RARGRGRAH SHNDHEFIS NTIHNKLVG 
      TVYDLDFDD DISSENVDL KSMRERRKS VDVQKTELK 
      ESSDSPSQK KYNDLRELR PPSPVKDTT PKPAEVEPI 
      FPDIVQPVL PGPVDMRTY NTNFDQSYN EANLLRAFD 
      SGTTEPPVN EEIDEEFEK ELQTALTTP SKKPDEPPA 
      NIKVSLSDS RNQLKVKIK GPIANYSST IPPLPPPVT 
      VSSISNNVI SNNSIGVSS GTSNLRRMR KKELLRQYW 
      TQDMNMDDS SCAPGFNAT PTVPPVNRT IITIPKAVA 
      SMTSIPTKD DYRDYRTDD VIETKQHRK EAKSRPGAL 
      SRELRQLDL SLDEDALHE RRRSIGSNN STNYDSGTN 
      KRRGRPPRP SQQTTGTPK IKIKIGNSI VGKIDDKRD 
      RIRPPKKRL ANINMPSVE DLKRESMKF RKLVMADFD 
      EGKEKRKKK DKSEKRKRK KAKPEVRII SNESQNKLI 
      IRIGKKGER TSGGEEGGA PIKLKLARC QEGSGYVMK 
      SEEAAQSPP PPPPPPPPT LGPPPLPLN KDCEVR
    CDS sequence
    • >TC006853 RA
      atgtcacag agagcaaaa aggcctaac cgctcagac 
      gatggatca gttcctgtt aaaaggcgc aaacgtacg 
      gcggcggag gaggaggaa gcggcctcc ttggcggtg 
      tggcagccc cgctccaac gacctgaag agcatctac 
      aaccggacg acgaccgag gcgccggcc gagctcttc 
      cgcaaagac ctgatcagt gcgatgaag ttgcccgat 
      tcggagccg ctggcatcg aatgagtac tgggttatt 
      gtcgatcag tggaagcag gagtgggaa aggggggtc 
      caggtgcca gtcaatcca gattccttg ccggaacct 
      agcgtcact gtcacacag aacacgcca ctcaagctg 
      caacatgag ttcaaactg ccaaaaaac aagtacatc 
      cgcatcacc aaggacgag aacttcaaa atcgaggag 
      cacgcccta tccaacgcc ccggctcaa tcggaggcg 
      gcttgctcg tacgacctg gacgaatgc gacgtctcc 
      tggctgcgc atcctcaac tcggagcgc tcccaatgc 
      ggccaatca ccgatcaac tccgagcaa ctcgagcgc 
      gtaatcgag caattcgag ctatgctgc tgggacaaa 
      atccagtcg attttacgc aacgaagaa ggcctgggc 
      atcgaatac gacgagaac gtcatctgc gacgtctgt 
      cgctccccc gactccgaa gaaggcaac gagatggtc 
      ttctgcgac agctgcaac atctgcgtc caccaggcc 
      tgctacggc atcacccgg atacccgaa ggccaatgg 
      ctgtgctgc acgtgccac ctcagcaag cggccgaaa 
      tgcgtgctc tgccccaac aaagggggc gccatgaag 
      tgcacgcgc tccggccag aagtgggcg cacgtctcg 
      tgcgccctc tggataccc gaagtgagc atcggctgt 
      gtggagaaa atggaaccc ataacgaaa atctcaagc 
      attccgcag agccgatgg gcgctgatc tgtgtcctg 
      tgccgggag agggtcggc gcctgcatc cagtgctcg 
      gtcaagacg tgcaaaacg gcgtatcat gtcacctgt 
      gcgttcaaa cacggcttg gaaatgcga gcgattatc 
      gaagacgag aacgccgat gacggagtt aagttgcgc 
      tcatattgc gagaaacac agcaagtcg agcaagaac 
      aaggagaag agtgtgtgt tcggggtcg gaggacgac 
      gattcgcgc cggaaaaag cgcaaagac atgacttcg 
      gaggagaag aaccaggca cgtgctgca cggttacag 
      gagatcgag tcggagttt ttcaagcac gtgtcgatt 
      aaagacgtc agtatccac ctggacgtg gacaacgac 
      gccctccac cacatctac aactactgg aagttgaag 
      cgcaaagcc gggaacaac aagccgctg ctgccgccg 
      aagtccgag gacgttgat atgctctct cataaacgc 
      gaacaggcc gatatggag aagatgaag atgtttgtg 
      caattgaga caggacttg gaacgggtg cggaatttg 
      tgttatatg gtgagtcgg agagagaaa ctgtcgagg 
      agtttcttt cgattgcgc gaacagacg ttttataaa 
      cagacggct gtgcttgaa gctgctaac aatttgccg 
      aatgttgtg gtacaagct gtgattgaa gccaatcat 
      ggaccgtcg atctatgat cggttgtat tcgcatgaa 
      gatgcagag gatcatacg agcgatttt gagaggatt 
      ttgacgagg attgctggg gttaagtcg ccgacggat 
      tcaggggat gagaaacgg acagagctt aatgggctt 
      tttaaggac aagaatcca tacaaaaag atttacttc 
      aatggttct tcgaatcga cgcagcggc agtttgtac 
      ggcagtagt tcgagtagt gacgaaaag gaccgcttg 
      aacgcggca acaacttcc gaagatgaa gacaaaagt 
      aaaaagaaa tcatcttca tcggtccga aaacctaac 
      aacaataac aacaaagcg tccgatcgc cgacgaaaa 
      agacacagt gcatctagt aaaatcgaa agtagtagt 
      gaggaggat tccaaacag aaaacgaaa aagacttgg 
      ggacaattc tcccgctta aagcaagtg gaacgcgaa 
      ttaggttat tcgggtagt gacagtgac gaactaatg 
      ccaatacgt gcaagccaa aaatcagac actaaacaa 
      atgtcgtca atctattcg gatagtgac agttcggac 
      gtatcaatg accaaagcg agtgattcc caacaaaat 
      aaactacga acgaaagca gcagtcaaa gagttttca 
      cacaaaccg tcaacgtcc aacaaaaca agtcccgaa 
      aacaaaaag aacaagaaa aaagaaagc cccacaaag 
      aaaaaagat tacgaccca tctgatctc attgtaccg 
      cagcgacaa gcggccaaa aaagcaacc gaaaatctc 
      aaatcgaca acaacgcga gttgtcaaa gaacaaatg 
      gacgaacca agcgtaaaa gttgaagaa aaacccgcc 
      aaattgccc aaacctaaa gcaaaaact aaagaaacc 
      gtaaaggaa gtgaaagaa gtgaagaag gaatcagcg 
      gtgaaaacg agcgatgtg ttcgatctc gataaagaa 
      acggacaat gcggagatt ctcgcctac gttccgcaa 
      cgtcaagct gcgaaaaaa gccgctgaa cacataaaa 
      agtgggctg ggtaagccc gtggcgccc tctagtgtt 
      gacgaattg gagaaaaac aaattggag agtacagtg 
      gtcaagaaa aaggaggcg gaaaaacaa cagccggct 
      aaagtccca gaaagcaaa cgcaagtct tcctcaaac 
      agcagttcg tcttcctcg tctagttct tccacgtcg 
      tcttcgacg tcgtcgagt tcagatagt gatgatgag 
      gttccacca agtcagcaa caagcgcaa caaccgcga 
      tcggagagt cttttctcg aattactcg tcgaaagag 
      tgtcgggaa acggctaag cggacgaac gccagcaag 
      gactggccc tttcttgac aagggtgcc aagtctgca 
      gcagcagcg gcgtccagc tcaagcacc gactcgagt 
      gattctagc cgtcccacc tccccaatc atcacaaca 
      acaacaaca acaaaaact actagtccc gacaagaag 
      tcaacaact actcaaaga gaggaacag acgagaatg 
      cgaggaaaa ggcagacca gcaacaaca gcagctaga 
      gggaggccg aggcaatca tctaaatcc ggtgatcgg 
      gtcggagat gctaatgtc aggcttaga aatttgtct 
      gatagccaa cgtggcgat gctaagtgt cggaaggga 
      aaacaggat gaggtggcc gagaaagag acaaagtcc 
      gttaacaaa ctacagtct ccggtccgg aaaacggac 
      aaaaaacta accgaaagc aaagaatcg tcggaagtc 
      agtaatctc gaaaaggaa atactcgaa cgaaaagct 
      ggaaaacag ggctcatcc aaaacgaaa tcaactctc 
      gaccggtta ttcggttca ccacctgac aaaaaggac 
      gaaaagaaa ccgttaaac aaaacgaca aaaccgacc 
      aaacctgaa cgagtaaaa tcaacagta aaggaaaac 
      aaagagccg gaaaaaata gcatcaccc gtcaaaaaa 
      tcgccggtt aaaagcaaa aacatcgat gagattttc 
      acacaaaag actgtccta aacgagaat aaaatcgag 
      gttaaagaa aaggaaaaa cacccaact ccgaagcgt 
      gaagaaatc gaaccggcg ccaaaaatc gaagatttc 
      aaacaaaac aacgagaaa ctcaccgaa ccggagaag 
      aaattgccg cacagtcgg tcaattttc tcgccaccg 
      catctcaaa gacaatttg cttgatttc gataatttg 
      gacgacggt tttggtatt tccaaagac gaggagata 
      atgaaaggc cccctgacg ttcaatttc ggcaacact 
      agtcttttt aaggaagac tcaaaggaa gacagtgca 
      cgtgaaacg ctaaatctt gtcgaaaaa cttcgcatg 
      gaaatgtcg aaaaaatcg accagttgt gatgttgac 
      gatggtgca agtgtttcg tcgagtact aaaaacgaa 
      tgtgatcgg actgagttt gccgaacaa gtggtgccg 
      aattgtgtg gcggaaaag aacgatgcg aaagtggat 
      gatgtgccg ccgccgact gtgaacttg gagatgcgt 
      gtggaggtg acggaagcg aaaggtttc gccgataga 
      gggcaggac tatcggttt atgagttgt gaggcggat 
      gttccattg gaacagaac aagaacatt ccgattcaa 
      cagcatcat gggcttagt gatcatggg attagtggg 
      cagggtgat gagagatgg gtgccaccc agcgaggcg 
      tatacggca gcgccacct atagaccct tcgaattac 
      ggcagcgag agccaacaa cctcttcaa acccctcaa 
      cacttcatg cagcaatac tcactcaac aagactcaa 
      cccctcctc aacgccatt cctccccag atcgaaatg 
      cgcctccac gacgacacc ctcaaaaac ccatccccc 
      acgttacac gaccgtcga atctcacaa acccccctc 
      aacgaagac tgcatgccc agcccatcg ccgtacgcc 
      gacatccac ccccaggcc aaatgggcc gacagcgaa 
      atcatgccc aaccgacgt tcttcctcc agctcggcg 
      gcctccacc tccagctcg tgttcgcgg cgaaacgac 
      gacgacgac ctcaaactg cggcccgac gtcatgatg 
      gtcaaccac caggctctc gaaatcggc tacctgcac 
      ccgtttcca cccgaattg ttcccgact ttgtccgat 
      tcctcgcaa tttgtcagt ccggtaagt ctcctccat 
      cctcctcct ttgaacccc tcgctagcg tttccttcg 
      accgctccg gcgatgtta ccgccggct tttgcccca 
      tttccccca gctcaggtg atggcgccc acgctgtcc 
      aagccgctg gaggatgtg ccgcattgc acggccgcc 
      ttcacgtcc tcgcagcat aatatggcg ttgacggcg 
      gccattgcg aatctgccg ccgagtttg cccaaggaa 
      gtcgaggcg ctgcctgag gatgaggtc gttccgacg 
      actcagagt aatgttttg tcgtcacca agtccttca 
      attaagtcg aatagttcg gggaaaaaa tcgccaagt 
      aagccgaca cggacgtcg gcacgggtc acctcgcag 
      gggaagagc cccaagcaa gacgtggtc aagccgagc 
      gggagaggg cgaggtgaa gcgaagaga aattcggtt 
      aagtcgaat gctgcaaga gggcgtggc agggctaga 
      ggcaggggt agagcacac tctcacaat gatcatgag 
      tttatcagc aatacgatt cataataag ttggtgggg 
      acggtgtac gatctggac tttgatgac gacatttcg 
      agcgaaaac gtcgatttg aaatcgatg agggaacgc 
      cgaaagtca gtcgatgtc caaaagacc gaactgaag 
      gaaagttcc gactcacca tcgcagaaa aaatacaat 
      gacttgaga gagttgcga ccgccgagc ccagtcaag 
      gacacaacg ccgaaaccg gccgaagtc gaaccgatc 
      ttccccgac atcgtccag ccggtatta ccgggccct 
      gtagacatg cgcacctac aacaccaac ttcgaccaa 
      agctacaac gaagccaac ctcctccga gcgttcgac 
      tccgggaca acggaacca ccagtcaac gaggagatt 
      gacgaggag ttcgagaaa gaactccag acggctttg 
      acaactccg agtaaaaaa ccggacgaa cctcccgcc 
      aatatcaaa gtttcgtta tcggactct cgtaatcaa 
      ctcaaagtc aagattaag ggccccatc gccaactac 
      tcctcgacc ataccgccg ctacctccc ccggtcacc 
      gtttcgagc atttcgaac aacgtcatc agtaacaat 
      tcgatcggg gtcagttcg ggaacgtcg aacttgcga 
      cgcatgcgc aaaaaagaa ctgctacga cagtactgg 
      acgcaggat atgaacatg gatgattcg tcctgtgcg 
      cctggtttt aacgctact ccaaccgta cctcccgtc 
      aatcgtact attatcact atacctaag gctgtggct 
      tcaatgacc agtattccg acaaaagac gattatcgc 
      gactaccga accgacgat gtcatcgaa acaaaacaa 
      caccgcaag gaggccaag tcacgaccg ggcgccctc 
      tcgcgtgaa ctgcgccaa ctagacctg tccttggac 
      gaagacgcc ttgcatgaa cgccggcga agtatcgga 
      agcaacaat tcgacgaac tacgactct gggacgaac 
      aaacgaaga gggcgcccc ccgcgcccg tcgcagcaa 
      acaaccggg actccgaaa atcaaaatt aagattggc 
      aatagtata gttggtaag attgacgat aaacgggat 
      cggataaga ccgccgaag aaacgcctc gccaatata 
      aacatgccg agtgtcgaa gacctgaaa cgcgagagt 
      atgaagttc cggaagttg gtcatggcc gacttcgac 
      gagggtaag gagaagcgg aagaagaaa gacaaaagt 
      gagaaacgt aagcggaaa aaggcgaaa cccgaagtg 
      cggataatc tcgaatgag agccagaac aagttgatt 
      atacgtatc gggaaaaag ggtgagcgg actagtggt 
      ggtgaggag ggtggtgct ccgattaag ttgaagttg 
      gcgcggtgt caggagggc agcgggtat gtgatgaag 
      agcgaggag gcggcgcag tcgccgccg ccgccaccg 
      ccgccgccg cccccgacg ttgggcccg ccgccgctg 
      cccctcaac aaagactgt gaagttaga tga
    mRNA sequence
    • >TC006853 RA
      tgtcgatgg tggcgccag ctacattgt caatgttgt 
      taggcttac caaatggag tctaatgga gtcgattta 
      ataaaaatt gcctgaaaa tcaatcgaa acgatggaa 
      aacctcaaa gaaatggtt aggcagcgt cgacacact 
      gcctgcaca ataaggtgc gatgtcaca gagagcaaa 
      aaggcctaa ccgctcaga cgatggatc agttcctgt 
      taaaaggcg caaacgtac ggcggcgga ggaggagga 
      agcggcctc cttggcggt gtggcagcc ccgctccaa 
      cgacctgaa gagcatcta caaccggac gacgaccga 
      ggcgccggc cgagctctt ccgcaaaga cctgatcag 
      tgcgatgaa gttgcccga ttcggagcc gctggcatc 
      gaatgagta ctgggttat tgtcgatca gtggaagca 
      ggagtggga aaggggggt ccaggtgcc agtcaatcc 
      agattcctt gccggaacc tagcgtcac tgtcacaca 
      gaacacgcc actcaagct gcaacatga gttcaaact 
      gccaaaaaa caagtacat ccgcatcac caaggacga 
      gaacttcaa aatcgagga gcacgccct atccaacgc 
      cccggctca atcggaggc ggcttgctc gtacgacct 
      ggacgaatg cgacgtctc ctggctgcg catcctcaa 
      ctcggagcg ctcccaatg cggccaatc accgatcaa 
      ctccgagca actcgagcg cgtaatcga gcaattcga 
      gctatgctg ctgggacaa aatccagtc gattttacg 
      caacgaaga aggcctggg catcgaata cgacgagaa 
      cgtcatctg cgacgtctg tcgctcccc cgactccga 
      agaaggcaa cgagatggt cttctgcga cagctgcaa 
      catctgcgt ccaccaggc ctgctacgg catcacccg 
      gatacccga aggccaatg gctgtgctg cacgtgcca 
      cctcagcaa gcggccgaa atgcgtgct ctgccccaa 
      caaaggggg cgccatgaa gtgcacgcg ctccggcca 
      gaagtgggc gcacgtctc gtgcgccct ctggatacc 
      cgaagtgag catcggctg tgtggagaa aatggaacc 
      cataacgaa aatctcaag cattccgca gagccgatg 
      ggcgctgat ctgtgtcct gtgccggga gagggtcgg 
      cgcctgcat ccagtgctc ggtcaagac gtgcaaaac 
      ggcgtatca tgtcacctg tgcgttcaa acacggctt 
      ggaaatgcg agcgattat cgaagacga gaacgccga 
      tgacggagt taagttgcg ctcatattg cgagaaaca 
      cagcaagtc gagcaagaa caaggagaa gagtgtgtg 
      ttcggggtc ggaggacga cgattcgcg ccggaaaaa 
      gcgcaaaga catgacttc ggaggagaa gaaccaggc 
      acgtgctgc acggttaca ggagatcga gtcggagtt 
      tttcaagca cgtgtcgat taaagacgt cagtatcca 
      cctggacgt ggacaacga cgccctcca ccacatcta 
      caactactg gaagttgaa gcgcaaagc cgggaacaa 
      caagccgct gctgccgcc gaagtccga ggacgttga 
      tatgctctc tcataaacg cgaacaggc cgatatgga 
      gaagatgaa gatgtttgt gcaattgag acaggactt 
      ggaacgggt gcggaattt gtgttatat ggtgagtcg 
      gagagagaa actgtcgag gagtttctt tcgattgcg 
      cgaacagac gttttataa acagacggc tgtgcttga 
      agctgctaa caatttgcc gaatgttgt ggtacaagc 
      tgtgattga agccaatca tggaccgtc gatctatga 
      tcggttgta ttcgcatga agatgcaga ggatcatac 
      gagcgattt tgagaggat tttgacgag gattgctgg 
      ggttaagtc gccgacgga ttcagggga tgagaaacg 
      gacagagct taatgggct ttttaagga caagaatcc 
      atacaaaaa gatttactt caatggttc ttcgaatcg 
      acgcagcgg cagtttgta cggcagtag ttcgagtag 
      tgacgaaaa ggaccgctt gaacgcggc aacaacttc 
      cgaagatga agacaaaag taaaaagaa atcatcttc 
      atcggtccg aaaacctaa caacaataa caacaaagc 
      gtccgatcg ccgacgaaa aagacacag tgcatctag 
      taaaatcga aagtagtag tgaggagga ttccaaaca 
      gaaaacgaa aaagacttg gggacaatt ctcccgctt 
      aaagcaagt ggaacgcga attaggtta ttcgggtag 
      tgacagtga cgaactaat gccaatacg tgcaagcca 
      aaaatcaga cactaaaca aatgtcgtc aatctattc 
      ggatagtga cagttcgga cgtatcaat gaccaaagc 
      gagtgattc ccaacaaaa taaactacg aacgaaagc 
      agcagtcaa agagttttc acacaaacc gtcaacgtc 
      caacaaaac aagtcccga aaacaaaaa gaacaagaa 
      aaaagaaag ccccacaaa gaaaaaaga ttacgaccc 
      atctgatct cattgtacc gcagcgaca agcggccaa 
      aaaagcaac cgaaaatct caaatcgac aacaacgcg 
      agttgtcaa agaacaaat ggacgaacc aagcgtaaa 
      agttgaaga aaaacccgc caaattgcc caaacctaa 
      agcaaaaac taaagaaac cgtaaagga agtgaaaga 
      agtgaagaa ggaatcagc ggtgaaaac gagcgatgt 
      gttcgatct cgataaaga aacggacaa tgcggagat 
      tctcgccta cgttccgca acgtcaagc tgcgaaaaa 
      agccgctga acacataaa aagtgggct gggtaagcc 
      cgtggcgcc ctctagtgt tgacgaatt ggagaaaaa 
      caaattgga gagtacagt ggtcaagaa aaaggaggc 
      ggaaaaaca acagccggc taaagtccc agaaagcaa 
      acgcaagtc ttcctcaaa cagcagttc gtcttcctc 
      gtctagttc ttccacgtc gtcttcgac gtcgtcgag 
      ttcagatag tgatgatga ggttccacc aagtcagca 
      acaagcgca acaaccgcg atcggagag tcttttctc 
      gaattactc gtcgaaaga gtgtcggga aacggctaa 
      gcggacgaa cgccagcaa ggactggcc ctttcttga 
      caagggtgc caagtctgc agcagcagc ggcgtccag 
      ctcaagcac cgactcgag tgattctag ccgtcccac 
      ctccccaat catcacaac aacaacaac aacaaaaac 
      tactagtcc cgacaagaa gtcaacaac tactcaaag 
      agaggaaca gacgagaat gcgaggaaa aggcagacc 
      agcaacaac agcagctag agggaggcc gaggcaatc 
      atctaaatc cggtgatcg ggtcggaga tgctaatgt 
      caggcttag aaatttgtc tgatagcca acgtggcga 
      tgctaagtg tcggaaggg aaaacagga tgaggtggc 
      cgagaaaga gacaaagtc cgttaacaa actacagtc 
      tccggtccg gaaaacgga caaaaaact aaccgaaag 
      caaagaatc gtcggaagt cagtaatct cgaaaagga 
      aatactcga acgaaaagc tggaaaaca gggctcatc 
      caaaacgaa atcaactct cgaccggtt attcggttc 
      accacctga caaaaagga cgaaaagaa accgttaaa 
      caaaacgac aaaaccgac caaacctga acgagtaaa 
      atcaacagt aaaggaaaa caaagagcc ggaaaaaat 
      agcatcacc cgtcaaaaa atcgccggt taaaagcaa 
      aaacatcga tgagatttt cacacaaaa gactgtcct 
      aaacgagaa taaaatcga ggttaaaga aaaggaaaa 
      acacccaac tccgaagcg tgaagaaat cgaaccggc 
      gccaaaaat cgaagattt caaacaaaa caacgagaa 
      actcaccga accggagaa gaaattgcc gcacagtcg 
      gtcaatttt ctcgccacc gcatctcaa agacaattt 
      gcttgattt cgataattt ggacgacgg ttttggtat 
      ttccaaaga cgaggagat aatgaaagg ccccctgac 
      gttcaattt cggcaacac tagtctttt taaggaaga 
      ctcaaagga agacagtgc acgtgaaac gctaaatct 
      tgtcgaaaa acttcgcat ggaaatgtc gaaaaaatc 
      gaccagttg tgatgttga cgatggtgc aagtgtttc 
      gtcgagtac taaaaacga atgtgatcg gactgagtt 
      tgccgaaca agtggtgcc gaattgtgt ggcggaaaa 
      gaacgatgc gaaagtgga tgatgtgcc gccgccgac 
      tgtgaactt ggagatgcg tgtggaggt gacggaagc 
      gaaaggttt cgccgatag agggcagga ctatcggtt 
      tatgagttg tgaggcgga tgttccatt ggaacagaa 
      caagaacat tccgattca acagcatca tgggcttag 
      tgatcatgg gattagtgg gcagggtga tgagagatg 
      ggtgccacc cagcgaggc gtatacggc agcgccacc 
      tatagaccc ttcgaatta cggcagcga gagccaaca 
      acctcttca aacccctca acacttcat gcagcaata 
      ctcactcaa caagactca acccctcct caacgccat 
      tcctcccca gatcgaaat gcgcctcca cgacgacac 
      cctcaaaaa cccatcccc cacgttaca cgaccgtcg 
      aatctcaca aacccccct caacgaaga ctgcatgcc 
      cagcccatc gccgtacgc cgacatcca cccccaggc 
      caaatgggc cgacagcga aatcatgcc caaccgacg 
      ttcttcctc cagctcggc ggcctccac ctccagctc 
      gtgttcgcg gcgaaacga cgacgacga cctcaaact 
      gcggcccga cgtcatgat ggtcaacca ccaggctct 
      cgaaatcgg ctacctgca cccgtttcc acccgaatt 
      gttcccgac tttgtccga ttcctcgca atttgtcag 
      tccggtaag tctcctcca tcctcctcc tttgaaccc 
      ctcgctagc gtttccttc gaccgctcc ggcgatgtt 
      accgccggc ttttgcccc atttccccc agctcaggt 
      gatggcgcc cacgctgtc caagccgct ggaggatgt 
      gccgcattg cacggccgc cttcacgtc ctcgcagca 
      taatatggc gttgacggc ggccattgc gaatctgcc 
      gccgagttt gcccaagga agtcgaggc gctgcctga 
      ggatgaggt cgttccgac gactcagag taatgtttt 
      gtcgtcacc aagtccttc aattaagtc gaatagttc 
      ggggaaaaa atcgccaag taagccgac acggacgtc 
      ggcacgggt cacctcgca ggggaagag ccccaagca 
      agacgtggt caagccgag cgggagagg gcgaggtga 
      agcgaagag aaattcggt taagtcgaa tgctgcaag 
      agggcgtgg cagggctag aggcagggg tagagcaca 
      ctctcacaa tgatcatga gtttatcag caatacgat 
      tcataataa gttggtggg gacggtgta cgatctgga 
      ctttgatga cgacatttc gagcgaaaa cgtcgattt 
      gaaatcgat gagggaacg ccgaaagtc agtcgatgt 
      ccaaaagac cgaactgaa ggaaagttc cgactcacc 
      atcgcagaa aaaatacaa tgacttgag agagttgcg 
      accgccgag cccagtcaa ggacacaac gccgaaacc 
      ggccgaagt cgaaccgat cttccccga catcgtcca 
      gccggtatt accgggccc tgtagacat gcgcaccta 
      caacaccaa cttcgacca aagctacaa cgaagccaa 
      cctcctccg agcgttcga ctccgggac aacggaacc 
      accagtcaa cgaggagat tgacgagga gttcgagaa 
      agaactcca gacggcttt gacaactcc gagtaaaaa 
      accggacga acctcccgc caatatcaa agtttcgtt 
      atcggactc tcgtaatca actcaaagt caagattaa 
      gggccccat cgccaacta ctcctcgac cataccgcc 
      gctacctcc cccggtcac cgtttcgag catttcgaa 
      caacgtcat cagtaacaa ttcgatcgg ggtcagttc 
      gggaacgtc gaacttgcg acgcatgcg caaaaaaga 
      actgctacg acagtactg gacgcagga tatgaacat 
      ggatgattc gtcctgtgc gcctggttt taacgctac 
      tccaaccgt acctcccgt caatcgtac tattatcac 
      tatacctaa ggctgtggc ttcaatgac cagtattcc 
      gacaaaaga cgattatcg cgactaccg aaccgacga 
      tgtcatcga aacaaaaca acaccgcaa ggaggccaa 
      gtcacgacc gggcgccct ctcgcgtga actgcgcca 
      actagacct gtccttgga cgaagacgc cttgcatga 
      acgccggcg aagtatcgg aagcaacaa ttcgacgaa 
      ctacgactc tgggacgaa caaacgaag agggcgccc 
      cccgcgccc gtcgcagca aacaaccgg gactccgaa 
      aatcaaaat taagattgg caatagtat agttggtaa 
      gattgacga taaacggga tcggataag accgccgaa 
      gaaacgcct cgccaatat aaacatgcc gagtgtcga 
      agacctgaa acgcgagag tatgaagtt ccggaagtt 
      ggtcatggc cgacttcga cgagggtaa ggagaagcg 
      gaagaagaa agacaaaag tgagaaacg taagcggaa 
      aaaggcgaa acccgaagt gcggataat ctcgaatga 
      gagccagaa caagttgat tatacgtat cgggaaaaa 
      gggtgagcg gactagtgg tggtgagga gggtggtgc 
      tccgattaa gttgaagtt ggcgcggtg tcaggaggg 
      cagcgggta tgtgatgaa gagcgagga ggcggcgca 
      gtcgccgcc gccgccacc gccgccgcc gcccccgac 
      gttgggccc gccgccgct gcccctcaa caaagactg 
      tgaagttag atgatagtc gtgtaccat tgatctgta 
      atataagta taggtgacc agtgaggca ggttgtaca 
      gacgctagc tgctacttc acctgtttt aaatccacg 
      ttgttaagc tgtctagtg tattattat gatgtgtag 
      gctaagcga gcacatgta tgtcattaa tttcagtgt 
      acaaagtca attacagcc gcaaacgtg taaccatat 
      ttttgtaca tattgtaag ttatggtat ttgtgaata 
      taaatagta ctaatttat atatattaa tatcattta 
      aaatgatta actcgtgtt gacaatttt gatagctag 
      aatcgttat agagtttca tcagatatt gtacagaac 
      aaatggctt gagtgcaat ctttttatc ggttgtttg 
      ttgtgagag agccattat tttattatt gtacagtct 
      ttattattt taaacgtgc gtctagcga cttgtacta 
      taaat
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