Status
Please wait ...
Gene information
General information
TC006849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 168239..181879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006849
      iBeetle screen iB_04226

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006849 RA
        MLSCLGRLS PRRQEFLLY MAVSILTVN SVNVVLSEW 
        GLYFTDMTE LLKNHPNHM LIDYACFAT RIATAAVIL 
        VSLLDSQFA FCSMQIAPN KKDKKKLHG SHEQVSDIE 
        YIIPRPKSA KPPTVYNAY SQSWVFESD SASCSNTAE 
        SPYLKILEN GTPKPKSKK LQNGVVSDT THLIDNPVV 
        HIEEASDDS NSNSDRKLC HMRSFSRSA SPVLSGGSS 
        RVNLHQSST NPPIYDCLE SIYRTRLNT GPRDEEHYQ 
        APQAMVLRR VESVSPHAE KVQYASLMK ELQRAIVSK 
        KEPSVTSPS SDSKSSSKT SSDAEFSKE LEAALQLIQ 
        DLESPNTIE TPSVKERPL AVWQTSGSE SEKTLSAVG 
        SLAEIASPE GAPTNGKPA VPDSQSTSG YSSPSHPTP 
        SWSTTSSLN GSSTDMAYS IHNAHSGTV ISLYSQVNP 
        QCKGKSVTL VNINGDPTP IHDAVSTKS GEKNPGWRS 
        LLRKKKQPP KLCPELEGA IIKSESLAY LSELELLAR 
        HQRNKEIQR EIEERVIQQ LGTPRTESN C
      CDS sequence
      • >TC006849 RA
        atgttgtcc tgtttggga cgcttatcg ccccgacgc 
        caggaattc ctcctttac atggccgtc tcaatcctg 
        acagtgaac agcgtcaac gtcgtcctg agcgagtgg 
        gggctttac ttcacagac atgacggag ctgttgaaa 
        aatcatccc aatcacatg ctgattgac tacgcatgt 
        tttgccaca cggatcgcc acagccgcc gtcatctta 
        gtctccctt ctcgacagt caattcgct ttctgttct 
        atgcagata gcaccgaat aagaaagat aagaagaaa 
        ttgcacgga tcccacgaa caggtctcc gacattgag 
        tacatcata ccaaggcca aaatcagcc aaaccccca 
        accgtctac aacgcctac tcccaaagt tgggtgttc 
        gagtcggac agcgcaagt tgttcaaac accgccgaa 
        tcgccctac ttgaaaatc ctcgagaac ggcaccccc 
        aaacccaaa tcaaagaaa ctccaaaac ggcgtcgtg 
        agcgacacc acccacctc atcgataat cccgtagtg 
        cacatcgaa gaggcatcg gacgatagt aacagtaac 
        agtgatcgt aaactttgc cacatgcgt agtttctcg 
        cgctcggct tcgcctgtg ctctcgggc ggctcctcc 
        cgcgttaac ttgcaccaa agcagcacc aatccgccg 
        atctacgac tgcttggag agcatctac aggacgcgt 
        ctaaacacc ggcccccgg gacgaggag cactaccaa 
        gccccccag gccatggtg ctgcggcgg gtggagtcc 
        gtgagcccc cacgcggag aaggtccag tacgcctcg 
        ctgatgaag gagctgcag cgcgccatc gtcagcaag 
        aaggagccg tcggtgacg tcccccagc tccgactcc 
        aagagcagc tccaagacc agcagcgac gccgagttc 
        tccaaggag ctggaggcg gcgctccag ctgatccag 
        gacctggag tcgcccaac accatcgag acgccctcg 
        gtgaaggag cggcccctg gctgtgtgg cagaccagc 
        ggcagtgag agcgaaaag actctaagt gctgtgggg 
        tcgctggcg gagatcgcc tccccggaa ggggcgccc 
        acgaacggt aagcccgcc gtccccgac tcccagagc 
        acctccggg tacagctcc ccctcccat cccaccccc 
        agctggtcc accacctcc tccctcaac ggaagcagc 
        accgacatg gcctattcc attcataac gcgcattcc 
        ggcacagtc atctccctc tactcccaa gttaacccg 
        caatgcaaa ggcaaatca gtaacacta gtcaacata 
        aacggtgac cccaccccg attcacgac gctgttagt 
        accaaaagt ggggagaaa aacccgggc tggaggtcg 
        ctactgagg aagaagaaa cagccgccg aaactgtgc 
        ccggagtta gagggggct attattaag tcggagagt 
        ttggcgtat ctgtccgag cttgagttg ctagcgagg 
        catcagaga aataaagag atacaaagg gaaatcgaa 
        gaaagagta atccaacag ctgggtact ccaaggact 
        gagtcaaat tgctaa
      mRNA sequence
      • >TC006849 RA
        cagaacgtc ataatcggg attgttggc gggatgttg 
        tcctgtttg ggacgctta tcgccccga cgccaggaa 
        ttcctcctt tacatggcc gtctcaatc ctgacagtg 
        aacagcgtc aacgtcgtc ctgagcgag tgggggctt 
        tacttcaca gacatgacg gagctgttg aaaaatcat 
        cccaatcac atgctgatt gactacgca tgttttgcc 
        acacggatc gccacagcc gccgtcatc ttagtctcc 
        cttctcgac agtcaattc gctttctgt tctatgcag 
        atagcaccg aataagaaa gataagaag aaattgcac 
        ggatcccac gaacaggtc tccgacatt gagtacatc 
        ataccaagg ccaaaatca gccaaaccc ccaaccgtc 
        tacaacgcc tactcccaa agttgggtg ttcgagtcg 
        gacagcgca agttgttca aacaccgcc gaatcgccc 
        tacttgaaa atcctcgag aacggcacc cccaaaccc 
        aaatcaaag aaactccaa aacggcgtc gtgagcgac 
        accacccac ctcatcgat aatcccgta gtgcacatc 
        gaagaggca tcggacgat agtaacagt aacagtgat 
        cgtaaactt tgccacatg cgtagtttc tcgcgctcg 
        gcttcgcct gtgctctcg ggcggctcc tcccgcgtt 
        aacttgcac caaagcagc accaatccg ccgatctac 
        gactgcttg gagagcatc tacaggacg cgtctaaac 
        accggcccc cgggacgag gagcactac caagccccc 
        caggccatg gtgctgcgg cgggtggag tccgtgagc 
        ccccacgcg gagaaggtc cagtacgcc tcgctgatg 
        aaggagctg cagcgcgcc atcgtcagc aagaaggag 
        ccgtcggtg acgtccccc agctccgac tccaagagc 
        agctccaag accagcagc gacgccgag ttctccaag 
        gagctggag gcggcgctc cagctgatc caggacctg 
        gagtcgccc aacaccatc gagacgccc tcggtgaag 
        gagcggccc ctggctgtg tggcagacc agcggcagt 
        gagagcgaa aagactcta agtgctgtg gggtcgctg 
        gcggagatc gcctccccg gaaggggcg cccacgaac 
        ggtaagccc gccgtcccc gactcccag agcacctcc 
        gggtacagc tccccctcc catcccacc cccagctgg 
        tccaccacc tcctccctc aacggaagc agcaccgac 
        atggcctat tccattcat aacgcgcat tccggcaca 
        gtcatctcc ctctactcc caagttaac ccgcaatgc 
        aaaggcaaa tcagtaaca ctagtcaac ataaacggt 
        gaccccacc ccgattcac gacgctgtt agtaccaaa 
        agtggggag aaaaacccg ggctggagg tcgctactg 
        aggaagaag aaacagccg ccgaaactg tgcccggag 
        ttagagggg gctattatt aagtcggag agtttggcg 
        tatctgtcc gagcttgag ttgctagcg aggcatcag 
        agaaataaa gagatacaa agggaaatc gaagaaaga 
        gtaatccaa cagctgggt actccaagg actgagtca 
        aattgctaa aactcaatt agaaagtta gcgaaactt 
        aacgttgaa acccctcaa ctctgagct gcaggtcag 
        cgacgtcca ttttttcca attctacga tttatattt 
        tcgatactt gtacgtaaa aaaacatct ctatgtgta 
        catattgtt gttatagtt atatagttt atatcataa 
        ttctattgt aaatattac aataaaaag tccctttta 
        ta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY