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Gene information
General information
TC006840 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450512.1: 111408..120239
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006840
    iBeetle screen iB_04224

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages random partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006840 RA
      MDIKQEVLS NEEFSIDED GGVENEHFV LSSDSEDEK 
      LRDTYGIPK DLTFEFVDI ETAKKELSD QGFLLNHFK 
      QDGEVETVV CTCEETSQC LYHKLKVET IVATPLETT 
      PSLNHLNIK VEEQEISIP KPKQFKCDI CDKVLGLSG 
      KQRHLRIHN VTTSTTEKP PTCDICSRT FYSRANLER 
      HVKIHMGDK SYVCEFCGR SYTREDNFK GHLRLHTGE 
      KPYVCEYCG KSFKNKANL NIHRLRRHN NQSYVCEFC 
      SKSFREEKL LNLHKQNIH SEEKPFACT HCTERYNTM 
      LALTNHLET HGIQCENSL IDTMCPICN KVFFTKAIL 
      KTHLLYHGD KQFSCTFCT KKFCTENQL TVHTRIHTG 
      EKPYVCKPC NKSFNTMVS LKYHMYLHT GEKPHKCTF 
      CDKAFVQSH HLKRHMMLH TGEKPYSCP HCDKKSTCK 
      FSMECHVRI HTKEKPFKC DICSKAFRN PFYIRKHKQ 
      KEHQTDIA
    CDS sequence
    • >TC006840 RA
      atggatatt aaacaagaa gttttatcc aatgaagaa 
      ttttcaatt gatgaagac ggtggtgtt gaaaatgaa 
      cattttgtg ttgagtagt gatagtgaa gatgaaaaa 
      ctcagggac acttatggg attcccaaa gacttaacc 
      tttgagttt gttgatatt gaaacggca aagaaagaa 
      ttaagtgac caaggattc cttttaaat cattttaaa 
      caagatgga gaagttgaa acagttgtt tgcacttgt 
      gaggaaaca tctcagtgc ttgtatcat aaactaaaa 
      gttgaaaca attgttgcc actccactt gaaactact 
      ccaagctta aatcattta aatattaaa gttgaagag 
      caagaaatt tccataccg aagccaaaa caattcaag 
      tgtgacatt tgtgacaaa gtattgggt ttaagtgga 
      aagcagcgc catcttcgt atacataat gtgacaact 
      tcaacaaca gagaaaccc cccacttgt gacatttgt 
      tctcgtacc ttttattca agagcgaat ctagagaga 
      catgtaaag atacatatg ggagataaa tcgtatgtt 
      tgtgaattc tgtggtcgt agttataca agagaggac 
      aatttcaaa ggacacctc cgactacat acaggggaa 
      aaaccatac gtgtgtgaa tattgcggt aaaagtttc 
      aaaaataaa gcaaattta aatatccat cgactcaga 
      agacacaat aaccaatcg tatgtttgt gaattttgc 
      tcgaaaagt ttccgggag gagaaatta cttaattta 
      cataaacaa aatattcat tcggaggaa aaaccattt 
      gcttgtaca cattgtacc gaaagatat aacaccatg 
      ttggcactt acaaatcat ttggaaaca cacggtatt 
      caatgtgaa aacagccta attgacacg atgtgtcca 
      atttgtaat aaagtgttt ttcacaaaa gctatactg 
      aaaacacac ttgttgtac cacggagat aaacagttt 
      tcctgcacc ttttgtact aagaaattt tgtactgaa 
      aatcaactc acagtacac acaagaatc catacgggg 
      gaaaaaccg tacgtttgc aaaccttgt aacaaatca 
      ttcaacact atggtatcg cttaaatat catatgtat 
      ctccatact ggcgaaaaa cctcacaaa tgtacattt 
      tgcgataag gcttttgta cagagtcat catttaaag 
      cgtcatatg atgctccat actggtgaa aaaccctat 
      tcgtgtcca cattgtgac aaaaagtct acatgtaaa 
      ttttccatg gaatgtcat gtgaggatt catacgaag 
      gaaaaaccg ttcaagtgt gatatttgc tcgaaagcg 
      ttccgaaat cccttttat atccggaaa cataaacag 
      aaggaacat caaacggat attgcgtaa
    mRNA sequence
    • >TC006840 RA
      actaacctg taaaaaata ctatgtaga ccaaacaat 
      gaagcaaac tctcaagat taaaaaaaa cgactttgt 
      ctacaattg cctctcctg ttgatcgac aggttgtct 
      caaggccgt ttttgtgat aaattttga gggggctgt 
      ctgttcttc cgtactcta ggatataat caaaaggtc 
      gccgaaaat gcatcaaag aactcgccc agttttaca 
      ttttcaaaa agcgattta acaaatctg aaaaaacac 
      ttaaatgga tattaaaca agaagtttt atccaatga 
      agaattttc aattgatga agacggtgg tgttgaaaa 
      tgaacattt tgtgttgag tagtgatag tgaagatga 
      aaaactcag ggacactta tgggattcc caaagactt 
      aacctttga gtttgttga tattgaaac ggcaaagaa 
      agaattaag tgaccaagg attcctttt aaatcattt 
      taaacaaga tggagaagt tgaaacagt tgtttgcac 
      ttgtgagga aacatctca gtgcttgta tcataaact 
      aaaagttga aacaattgt tgccactcc acttgaaac 
      tactccaag cttaaatca tttaaatat taaagttga 
      agagcaaga aatttccat accgaagcc aaaacaatt 
      caagtgtga catttgtga caaagtatt gggtttaag 
      tggaaagca gcgccatct tcgtataca taatgtgac 
      aacttcaac aacagagaa accccccac ttgtgacat 
      ttgttctcg tacctttta ttcaagagc gaatctaga 
      gagacatgt aaagataca tatgggaga taaatcgta 
      tgtttgtga attctgtgg tcgtagtta tacaagaga 
      ggacaattt caaaggaca cctccgact acatacagg 
      ggaaaaacc atacgtgtg tgaatattg cggtaaaag 
      tttcaaaaa taaagcaaa tttaaatat ccatcgact 
      cagaagaca caataacca atcgtatgt ttgtgaatt 
      ttgctcgaa aagtttccg ggaggagaa attacttaa 
      tttacataa acaaaatat tcattcgga ggaaaaacc 
      atttgcttg tacacattg taccgaaag atataacac 
      catgttggc acttacaaa tcatttgga aacacacgg 
      tattcaatg tgaaaacag cctaattga cacgatgtg 
      tccaatttg taataaagt gtttttcac aaaagctat 
      actgaaaac acacttgtt gtaccacgg agataaaca 
      gttttcctg caccttttg tactaagaa attttgtac 
      tgaaaatca actcacagt acacacaag aatccatac 
      gggggaaaa accgtacgt ttgcaaacc ttgtaacaa 
      atcattcaa cactatggt atcgcttaa atatcatat 
      gtatctcca tactggcga aaaacctca caaatgtac 
      attttgcga taaggcttt tgtacagag tcatcattt 
      aaagcgtca tatgatgct ccatactgg tgaaaaacc 
      ctattcgtg tccacattg tgacaaaaa gtctacatg 
      taaattttc catggaatg tcatgtgag gattcatac 
      gaaggaaaa accgttcaa gtgtgatat ttgctcgaa 
      agcgttccg aaatccctt ttatatccg gaaacataa 
      acagaagga acatcaaac ggatattgc gtaaaataa 
      acatttatt tagttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY