| General information | |||
| TC030971
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450512.1: 88976..109668 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC030971 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04223 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC030971 RA
MMHNRVHTG EKQNVCKSC GRTFNSVPG LQHHMYFHT GEKPYECKM CDKAYVLAE NLKYHMMVH TGEKPYSCP HCDKKSRSK FDMECHIRI HTGEKPFKC DTCSKAFRN PRYLKRGCL VFHTPGYNQ VKMAILRQL LTKAMKPEI LQAPEIGKT DFDNLCLAC LSKDCLRNV FVITYEGAA LISLLEDLL NFHVAAGDD LPKKICKKC VEEIIQFYV FKKRFNETQ TKLQRVLIE KDDKVVSSN GQFSRDEEF IVKDSDVEN EVFVLSSDT EDDALRDFY GIPKDLDIV FVDIETAKK ELNSEGFVL KHLEQDGEV KTIVCTCDG ASQCLYHLK VKEQEISKV EVETVVPIL DLNHSSIKD KPEEKEVET IVSTSRKSS PNLNNSNSE DKEQEIPEQ FKCNICDKI FNLRVKKQH LCTHNIKEK PPTCQICSR VFRFRKHLR EHMRVHTGE KPFVCEFCG LRFTQNRSL KSHFLLHTG EKPYVCEFC GRSFSRKDY LTEHLHRHT GKKQYVCEC CGNGFITNP MLKKHLLVH NIGYVCEFC SKAFRYEKS LNSHKQNRH SEEKPFTCT HCNNRFCTI LALTNHLKT HGIQREIQQ SENLCQICD KEFFSKHRL KSHLLNHGN QKFITRGNQ KLSTRGNQK LLIHGDQKF SCTSCDKKF YTQAQLTIH NRVHTGEKE NLCKYCGQI FTSGNGLLQ HMYLHTGEK PYECKICDK AFVQSHHLK HHMMVHTDE KPYACTHCD KQFRSKFNL ECHVRLHTG EKPFKCDTC SKAFRNPIY LGRHKKKAH
- >TC030971 RB
MMHNRVHTG EKQNVCKSC GRTFNSVPG LQHHMYFHT GEKPYECKM CDKAYVLAE NLKYHMMVH TGEKPYSCP HCDKKSRSK FDMECHIRI HTGEKPFKC DTCSKAFRN PRYLKRGCL VFHTPGYNQ VKMAILRQL LTKAMKPEI LQAPEIGKT DFDNLCLAC LSKDCLRNV FVITYEGAA LISLLEDLL NFHVSNCHF RKVLL
- >TC030971 RA
atgatgcac aacagagtc catacagga gaaaaacaa aacgtttgt aagtcttgt ggacgaaca ttcaactct gtgccggga ctccaacat catatgtat ttccacact ggggaaaaa ccgtacgaa tgtaaaatg tgcgataaa gcttatgtc ctggctgaa aatttaaaa tatcatatg atggttcat actggtgaa aaaccatat tcgtgtcca cattgtgat aaaaaatct aggagcaaa tttgacatg gaatgccac attaggatt catacgggg gaaaaaccg ttcaaatgt gacacctgt tcgaaagcg ttccggaac ccgagatat ctcaaaagg ggctgcctt gtcttccat actccagga tataatcaa gtcaagatg gccatattg agacaatta ttaaccaaa gcaatgaag ccagaaata ttgcaagcc cccgaaatt ggaaaaacc gattttgac aatttgtgt ctcgcttgt ttatccaaa gattgtctc aggaatgtt tttgtgata acatacgag ggggctgcc ttaatatca ctactcgag gatttactc aactttcac gtcgcagca ggcgatgac ttgcctaaa aaaatctgc aaaaaatgc gtcgaagaa atcatccag ttttacgtc tttaaaaaa cgatttaac gaaactcaa acaaagcta caaagagtt ttgatagaa aaggatgat aaagtagtt tcatctaat ggacaattt tcacgtgat gaagaattt attgttaaa gacagtgat gtggaaaat gaagttttt gtgttgagt agtgatact gaagatgat gcacttagg gacttttat gggattccc aaagactta gacattgtg tttgtcgat attgaaacg gcaaagaag gaattaaat agcgaagga tttgtttta aagcatttg gaacaagat ggagaagtt aagacaatt gtttgtacc tgtgatgga gcatctcag tgcttatat catttgaaa gtaaaagaa caagaaatt tccaaagta gaagttgaa acagttgtt cccattcta gacttaaat cattcaagc attaaagat aaaccagaa gaaaaagaa gttgaaaca attgtctcc acctctagg aaatcttct ccaaactta aataattca aacagtgaa gataaagag caagaaatt ccggaacaa ttcaagtgt aacatttgt gacaaaata ttcaattta agagtaaag aagcaacat ctttgtaca cataacata aaagagaaa cctcccact tgtcaaatt tgttctcgt gtatttcgt tttagaaag catttaagg gaacatatg agagtacac acgggggaa aaaccgttt gtttgtgaa ttctgtggt ttgagattt acacaaaat cgtagtcta aagtcacac tttctacta catacagga gaaaagccg tacgtttgt gaattctgt ggtcgcagt ttttcacga aaggactat ctaactgaa cacctacat cgccataca ggaaaaaaa caatatgtg tgtgaatgt tgcggaaac ggtttcata actaatcca atgttaaaa aagcatcta cttgtacac aatataggg tacgtttgt gaattttgt tcgaaagct ttccgatat gagaaatca cttaattca cacaaacaa aatagacat tcggaggaa aaaccgttt acttgtaca cactgtaac aatagattt tgcaccata ttggcactt acaaaccat ctgaaaact catggtatt caacgtgaa atccagcaa agtgagaac ttgtgtcaa atttgtgat aaggaattt ttctcaaaa cacagatta aaatcacac ttgttgaat cacggaaac caaaagttc ataactcgc ggaaaccaa aagttgtca actcgcgga aaccaaaag ttgttgatt cacggagac cagaagttt tcctgtacc tcttgcgat aagaagttt tacactcaa gctcaactc acgatacat aatagagtc catacagga gaaaaagaa aacctttgt aaatattgt ggtcaaata ttcaccagt gggaatgga cttctacag catatgtat ctccatact ggcgaaaaa ccatacgaa tgtaaaatt tgcgataaa gcttttgtc cagagtcat catttaaaa catcatatg atggttcat actgatgaa aaaccctat gcgtgcaca cattgtgac aaacaattt agaagcaaa tttaacttg gaatgtcat gtcaggctt catacgggg gaaaaaccg tttaaatgt gacacctgt tccaaagcg ttccgaaac ccgatttat ctcggaagg cataagaag aaggcacat taa
- >TC030971 RB
atgatgcac aacagagtc catacagga gaaaaacaa aacgtttgt aagtcttgt ggacgaaca ttcaactct gtgccggga ctccaacat catatgtat ttccacact ggggaaaaa ccgtacgaa tgtaaaatg tgcgataaa gcttatgtc ctggctgaa aatttaaaa tatcatatg atggttcat actggtgaa aaaccatat tcgtgtcca cattgtgat aaaaaatct aggagcaaa tttgacatg gaatgccac attaggatt catacgggg gaaaaaccg ttcaaatgt gacacctgt tcgaaagcg ttccggaac ccgagatat ctcaaaagg ggctgcctt gtcttccat actccagga tataatcaa gtcaagatg gccatattg agacaatta ttaaccaaa gcaatgaag ccagaaata ttgcaagcc cccgaaatt ggaaaaacc gattttgac aatttgtgt ctcgcttgt ttatccaaa gattgtctc aggaatgtt tttgtgata acatacgag ggggctgcc ttaatatca ctactcgag gatttactc aactttcac gtaagtaac tgccatttc aggaaagtt ttactttag
- >TC030971 RA
ttatttctc caataaacc tcaaaaaaa acgtaaaac tattaattc aaagttgca ctctcaaga ttcgaaaaa ctgacattg tctactttt acttctcca atttatata gctagagat tgtctcgtg tgcgttttt gtgataaat ttcaggggg gctgacttg tcttccata ctccaggat acaaacatt attcaagtc gctgttgat gcgttgccg aagaaaatg tgtgaagtc gtccagttt tacatcttc aagtagcga tttcaacac tacaaagtg tttaaccga aatggataa taaaatagt ttcatgcaa cgaagaata tgaatattc aagtgatga agaaagtga agttgaaga tgaaccttt tgtgttgag tagtgatag tgaagatga tgcaattag ggacattta tgggattcc caaagactt aaacattgt gtttgtcga tattgaaac ggcaaagaa agaactaaa tagcaaagg attcgtttt aaagcattt ggaacaaga cggagaggt cgagagaat tgtttgcac ttgtgatgg agcatctca gtgcttata tcatatcaa aatagaaga gccattaaa cattaaaga agaagagcc agaaattcc caaaataga agttgaaac aacaagctt aaatgaact taaaactaa aatgaaact taaacttaa aaatgaacc agaagaaaa aaaatttga aacaattgc ctccacctc taggatatc ttctccaaa attaaataa ttcaaatat caaagatag agaacaaaa aaatccgaa accaaagcg aaaacaata caagtgtaa gatttgtga caaaatatt aaacttatc aggaaagta tcatcatct tcgtaccca caaaaaacc gtttgtttg tgaattctg tggtttgag attttcact aaaaggtag tctaaaggc acaccttct acaacatac aggagaaaa accgcacgt ttgtgaatt ctgtggtcg cagtttttc acgaaaaga ctatctaac ggaacacct acatcgcca tacaggaaa aaaaccaat tgtgtgtga acattgtgg taaaagttt cagaactaa gccagtttt aaaaacaca tctacttgt acacaatat agggtacgg ttgtgaatt ttgttcgaa agttttccg atatgagaa atcacttaa ttcacacaa acaaaatat acattcgga ggaaaaacc gtttgtttg tacacattg taaccaaag atattgcac cttattggc atttacaaa tcatctgga aactcatgg tattaaacg taaaatcca cgaaagtgg cccattgtt atgtcaaat ttgtgataa agaattttt gacaaaaaa cagattaaa atcacactt gttgacaca cggagacca aaagttttc ctgtagctt ttgcgctaa ggcatttaa cactaaagc tcgacttat gatgcacaa cagagtcca tacaggaga aaaacaaaa cgtttgtaa gtcttgtgg acgaacatt caactctgt gccgggact ccaacatca tatgtattt ccacactgg ggaaaaacc gtacgaatg taaaatgtg cgataaagc ttatgtcct ggctgaaaa tttaaaata tcatatgat ggttcatac tggtgaaaa accatattc gtgtccaca ttgtgataa aaaatctag gagcaaatt tgacatgga atgccacat taggattca tacggggga aaaaccgtt caaatgtga cacctgttc gaaagcgtt ccggaaccc gagatatct caaaagggg ctgccttgt cttccatac tccaggata taatcaagt caagatggc catattgag acaattatt aaccaaagc aatgaagcc agaaatatt gcaagcccc cgaaattgg aaaaaccga ttttgacaa tttgtgtct cgcttgttt atccaaaga ttgtctcag gaatgtttt tgtgataac atacgaggg ggctgcctt aatatcact actcgagga tttactcaa ctttcacgt cgcagcagg cgatgactt gcctaaaaa aatctgcaa aaaatgcgt cgaagaaat catccagtt ttacgtctt taaaaaacg atttaacga aactcaaac aaagctaca aagagtttt gatagaaaa ggatgataa agtagtttc atctaatgg acaattttc acgtgatga agaatttat tgttaaaga cagtgatgt ggaaaatga agtttttgt gttgagtag tgatactga agatgatgc acttaggga cttttatgg gattcccaa agacttaga cattgtgtt tgtcgatat tgaaacggc aaagaagga attaaatag cgaaggatt tgttttaaa gcatttgga acaagatgg agaagttaa gacaattgt ttgtacctg tgatggagc atctcagtg cttatatca tttgaaagt aaaagaaca agaaatttc caaagtaga agttgaaac agttgttcc cattctaga cttaaatca ttcaagcat taaagataa accagaaga aaaagaagt tgaaacaat tgtctccac ctctaggaa atcttctcc aaacttaaa taattcaaa cagtgaaga taaagagca agaaattcc ggaacaatt caagtgtaa catttgtga caaaatatt caatttaag agtaaagaa gcaacatct ttgtacaca taacataaa agagaaacc tcccacttg tcaaatttg ttctcgtgt atttcgttt tagaaagca tttaaggga acatatgag agtacacac gggggaaaa accgtttgt ttgtgaatt ctgtggttt gagatttac acaaaatcg tagtctaaa gtcacactt tctactaca tacaggaga aaagccgta cgtttgtga attctgtgg tcgcagttt ttcacgaaa ggactatct aactgaaca cctacatcg ccatacagg aaaaaaaca atatgtgtg tgaatgttg cggaaacgg tttcataac taatccaat gttaaaaaa gcatctact tgtacacaa tatagggta cgtttgtga attttgttc gaaagcttt ccgatatga gaaatcact taattcaca caaacaaaa tagacattc ggaggaaaa accgtttac ttgtacaca ctgtaacaa tagattttg caccatatt ggcacttac aaaccatct gaaaactca tggtattca acgtgaaat ccagcaaag tgagaactt gtgtcaaat ttgtgataa ggaattttt ctcaaaaca cagattaaa atcacactt gttgaatca cggaaacca aaagttcat aactcgcgg aaaccaaaa gttgtcaac tcgcggaaa ccaaaagtt gttgattca cggagacca gaagttttc ctgtacctc ttgcgataa gaagtttta cactcaagc tcaactcac gatacataa tagagtcca tacaggaga aaaagaaaa cctttgtaa atattgtgg tcaaatatt caccagtgg gaatggact tctacagca tatgtatct ccatactgg cgaaaaacc atacgaatg taaaatttg cgataaagc ttttgtcca gagtcatca tttaaaaca tcatatgat ggttcatac tgatgaaaa accctatgc gtgcacaca ttgtgacaa acaatttag aagcaaatt taacttgga atgtcatgt caggcttca tacggggga aaaaccgtt taaatgtga cacctgttc caaagcgtt ccgaaaccc gatttatct cggaaggca taagaagaa ggcacatta agacaattt tgcataaaa taaaaatgt attgtcttg
- >TC030971 RB
ttatttctc caataaacc tcaaaaaaa acgtaaaac tattaattc aaagttgca ctctcaaga ttcgaaaaa ctgacattg tctactttt acttctcca atttatata gctagagat tgtctcgtg tgcgttttt gtgataaat ttcaggggg gctgacttg tcttccata ctccaggat acaaacatt attcaagtc gctgttgat gcgttgccg aagaaaatg tgtgaagtc gtccagttt tacatcttc aagtagcga tttcaacac tacaaagtg tttaaccga aatggataa taaaatagt ttcatgcaa cgaagaata tgaatattc aagtgatga agaaagtga agttgaaga tgaaccttt tgtgttgag tagtgatag tgaagatga tgcaattag ggacattta tgggattcc caaagactt aaacattgt gtttgtcga tattgaaac ggcaaagaa agaactaaa tagcaaagg attcgtttt aaagcattt ggaacaaga cggagaggt cgagagaat tgtttgcac ttgtgatgg agcatctca gtgcttata tcatatcaa aatagaaga gccattaaa cattaaaga agaagagcc agaaattcc caaaataga agttgaaac aacaagctt aaatgaact taaaactaa aatgaaact taaacttaa aaatgaacc agaagaaaa aaaatttga aacaattgc ctccacctc taggatatc ttctccaaa attaaataa ttcaaatat caaagatag agaacaaaa aaatccgaa accaaagcg aaaacaata caagtgtaa gatttgtga caaaatatt aaacttatc aggaaagta tcatcatct tcgtaccca caaaaaacc gtttgtttg tgaattctg tggtttgag attttcact aaaaggtag tctaaaggc acaccttct acaacatac aggagaaaa accgcacgt ttgtgaatt ctgtggtcg cagtttttc acgaaaaga ctatctaac ggaacacct acatcgcca tacaggaaa aaaaccaat tgtgtgtga acattgtgg taaaagttt cagaactaa gccagtttt aaaaacaca tctacttgt acacaatat agggtacgg ttgtgaatt ttgttcgaa agttttccg atatgagaa atcacttaa ttcacacaa acaaaatat acattcgga ggaaaaacc gtttgtttg tacacattg taaccaaag atattgcac cttattggc atttacaaa tcatctgga aactcatgg tattaaacg taaaatcca cgaaagtgg cccattgtt atgtcaaat ttgtgataa agaattttt gacaaaaaa cagattaaa atcacactt gttgacaca cggagacca aaagttttc ctgtagctt ttgcgctaa ggcatttaa cactaaagc tcgacttat gatgcacaa cagagtcca tacaggaga aaaacaaaa cgtttgtaa gtcttgtgg acgaacatt caactctgt gccgggact ccaacatca tatgtattt ccacactgg ggaaaaacc gtacgaatg taaaatgtg cgataaagc ttatgtcct ggctgaaaa tttaaaata tcatatgat ggttcatac tggtgaaaa accatattc gtgtccaca ttgtgataa aaaatctag gagcaaatt tgacatgga atgccacat taggattca tacggggga aaaaccgtt caaatgtga cacctgttc gaaagcgtt ccggaaccc gagatatct caaaagggg ctgccttgt cttccatac tccaggata taatcaagt caagatggc catattgag acaattatt aaccaaagc aatgaagcc agaaatatt gcaagcccc cgaaattgg aaaaaccga ttttgacaa tttgtgtct cgcttgttt atccaaaga ttgtctcag gaatgtttt tgtgataac atacgaggg ggctgcctt aatatcact actcgagga tttactcaa ctttcacgt aagtaactg ccatttcag gaaagtttt actttagcc acactaaac gtatctgct taaa
