Status
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Gene information
General information
TC030971
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006838
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450512.1: 88976..109668
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030971
    iBeetle screen iB_04223

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030971 RA
      MMHNRVHTG EKQNVCKSC GRTFNSVPG LQHHMYFHT 
      GEKPYECKM CDKAYVLAE NLKYHMMVH TGEKPYSCP 
      HCDKKSRSK FDMECHIRI HTGEKPFKC DTCSKAFRN 
      PRYLKRGCL VFHTPGYNQ VKMAILRQL LTKAMKPEI 
      LQAPEIGKT DFDNLCLAC LSKDCLRNV FVITYEGAA 
      LISLLEDLL NFHVAAGDD LPKKICKKC VEEIIQFYV 
      FKKRFNETQ TKLQRVLIE KDDKVVSSN GQFSRDEEF 
      IVKDSDVEN EVFVLSSDT EDDALRDFY GIPKDLDIV 
      FVDIETAKK ELNSEGFVL KHLEQDGEV KTIVCTCDG 
      ASQCLYHLK VKEQEISKV EVETVVPIL DLNHSSIKD 
      KPEEKEVET IVSTSRKSS PNLNNSNSE DKEQEIPEQ 
      FKCNICDKI FNLRVKKQH LCTHNIKEK PPTCQICSR 
      VFRFRKHLR EHMRVHTGE KPFVCEFCG LRFTQNRSL 
      KSHFLLHTG EKPYVCEFC GRSFSRKDY LTEHLHRHT 
      GKKQYVCEC CGNGFITNP MLKKHLLVH NIGYVCEFC 
      SKAFRYEKS LNSHKQNRH SEEKPFTCT HCNNRFCTI 
      LALTNHLKT HGIQREIQQ SENLCQICD KEFFSKHRL 
      KSHLLNHGN QKFITRGNQ KLSTRGNQK LLIHGDQKF 
      SCTSCDKKF YTQAQLTIH NRVHTGEKE NLCKYCGQI 
      FTSGNGLLQ HMYLHTGEK PYECKICDK AFVQSHHLK 
      HHMMVHTDE KPYACTHCD KQFRSKFNL ECHVRLHTG 
      EKPFKCDTC SKAFRNPIY LGRHKKKAH
    • >TC030971 RB
      MMHNRVHTG EKQNVCKSC GRTFNSVPG LQHHMYFHT 
      GEKPYECKM CDKAYVLAE NLKYHMMVH TGEKPYSCP 
      HCDKKSRSK FDMECHIRI HTGEKPFKC DTCSKAFRN 
      PRYLKRGCL VFHTPGYNQ VKMAILRQL LTKAMKPEI 
      LQAPEIGKT DFDNLCLAC LSKDCLRNV FVITYEGAA 
      LISLLEDLL NFHVSNCHF RKVLL
    CDS sequence
    • >TC030971 RA
      atgatgcac aacagagtc catacagga gaaaaacaa 
      aacgtttgt aagtcttgt ggacgaaca ttcaactct 
      gtgccggga ctccaacat catatgtat ttccacact 
      ggggaaaaa ccgtacgaa tgtaaaatg tgcgataaa 
      gcttatgtc ctggctgaa aatttaaaa tatcatatg 
      atggttcat actggtgaa aaaccatat tcgtgtcca 
      cattgtgat aaaaaatct aggagcaaa tttgacatg 
      gaatgccac attaggatt catacgggg gaaaaaccg 
      ttcaaatgt gacacctgt tcgaaagcg ttccggaac 
      ccgagatat ctcaaaagg ggctgcctt gtcttccat 
      actccagga tataatcaa gtcaagatg gccatattg 
      agacaatta ttaaccaaa gcaatgaag ccagaaata 
      ttgcaagcc cccgaaatt ggaaaaacc gattttgac 
      aatttgtgt ctcgcttgt ttatccaaa gattgtctc 
      aggaatgtt tttgtgata acatacgag ggggctgcc 
      ttaatatca ctactcgag gatttactc aactttcac 
      gtcgcagca ggcgatgac ttgcctaaa aaaatctgc 
      aaaaaatgc gtcgaagaa atcatccag ttttacgtc 
      tttaaaaaa cgatttaac gaaactcaa acaaagcta 
      caaagagtt ttgatagaa aaggatgat aaagtagtt 
      tcatctaat ggacaattt tcacgtgat gaagaattt 
      attgttaaa gacagtgat gtggaaaat gaagttttt 
      gtgttgagt agtgatact gaagatgat gcacttagg 
      gacttttat gggattccc aaagactta gacattgtg 
      tttgtcgat attgaaacg gcaaagaag gaattaaat 
      agcgaagga tttgtttta aagcatttg gaacaagat 
      ggagaagtt aagacaatt gtttgtacc tgtgatgga 
      gcatctcag tgcttatat catttgaaa gtaaaagaa 
      caagaaatt tccaaagta gaagttgaa acagttgtt 
      cccattcta gacttaaat cattcaagc attaaagat 
      aaaccagaa gaaaaagaa gttgaaaca attgtctcc 
      acctctagg aaatcttct ccaaactta aataattca 
      aacagtgaa gataaagag caagaaatt ccggaacaa 
      ttcaagtgt aacatttgt gacaaaata ttcaattta 
      agagtaaag aagcaacat ctttgtaca cataacata 
      aaagagaaa cctcccact tgtcaaatt tgttctcgt 
      gtatttcgt tttagaaag catttaagg gaacatatg 
      agagtacac acgggggaa aaaccgttt gtttgtgaa 
      ttctgtggt ttgagattt acacaaaat cgtagtcta 
      aagtcacac tttctacta catacagga gaaaagccg 
      tacgtttgt gaattctgt ggtcgcagt ttttcacga 
      aaggactat ctaactgaa cacctacat cgccataca 
      ggaaaaaaa caatatgtg tgtgaatgt tgcggaaac 
      ggtttcata actaatcca atgttaaaa aagcatcta 
      cttgtacac aatataggg tacgtttgt gaattttgt 
      tcgaaagct ttccgatat gagaaatca cttaattca 
      cacaaacaa aatagacat tcggaggaa aaaccgttt 
      acttgtaca cactgtaac aatagattt tgcaccata 
      ttggcactt acaaaccat ctgaaaact catggtatt 
      caacgtgaa atccagcaa agtgagaac ttgtgtcaa 
      atttgtgat aaggaattt ttctcaaaa cacagatta 
      aaatcacac ttgttgaat cacggaaac caaaagttc 
      ataactcgc ggaaaccaa aagttgtca actcgcgga 
      aaccaaaag ttgttgatt cacggagac cagaagttt 
      tcctgtacc tcttgcgat aagaagttt tacactcaa 
      gctcaactc acgatacat aatagagtc catacagga 
      gaaaaagaa aacctttgt aaatattgt ggtcaaata 
      ttcaccagt gggaatgga cttctacag catatgtat 
      ctccatact ggcgaaaaa ccatacgaa tgtaaaatt 
      tgcgataaa gcttttgtc cagagtcat catttaaaa 
      catcatatg atggttcat actgatgaa aaaccctat 
      gcgtgcaca cattgtgac aaacaattt agaagcaaa 
      tttaacttg gaatgtcat gtcaggctt catacgggg 
      gaaaaaccg tttaaatgt gacacctgt tccaaagcg 
      ttccgaaac ccgatttat ctcggaagg cataagaag 
      aaggcacat taa
    • >TC030971 RB
      atgatgcac aacagagtc catacagga gaaaaacaa 
      aacgtttgt aagtcttgt ggacgaaca ttcaactct 
      gtgccggga ctccaacat catatgtat ttccacact 
      ggggaaaaa ccgtacgaa tgtaaaatg tgcgataaa 
      gcttatgtc ctggctgaa aatttaaaa tatcatatg 
      atggttcat actggtgaa aaaccatat tcgtgtcca 
      cattgtgat aaaaaatct aggagcaaa tttgacatg 
      gaatgccac attaggatt catacgggg gaaaaaccg 
      ttcaaatgt gacacctgt tcgaaagcg ttccggaac 
      ccgagatat ctcaaaagg ggctgcctt gtcttccat 
      actccagga tataatcaa gtcaagatg gccatattg 
      agacaatta ttaaccaaa gcaatgaag ccagaaata 
      ttgcaagcc cccgaaatt ggaaaaacc gattttgac 
      aatttgtgt ctcgcttgt ttatccaaa gattgtctc 
      aggaatgtt tttgtgata acatacgag ggggctgcc 
      ttaatatca ctactcgag gatttactc aactttcac 
      gtaagtaac tgccatttc aggaaagtt ttactttag
    mRNA sequence
    • >TC030971 RA
      ttatttctc caataaacc tcaaaaaaa acgtaaaac 
      tattaattc aaagttgca ctctcaaga ttcgaaaaa 
      ctgacattg tctactttt acttctcca atttatata 
      gctagagat tgtctcgtg tgcgttttt gtgataaat 
      ttcaggggg gctgacttg tcttccata ctccaggat 
      acaaacatt attcaagtc gctgttgat gcgttgccg 
      aagaaaatg tgtgaagtc gtccagttt tacatcttc 
      aagtagcga tttcaacac tacaaagtg tttaaccga 
      aatggataa taaaatagt ttcatgcaa cgaagaata 
      tgaatattc aagtgatga agaaagtga agttgaaga 
      tgaaccttt tgtgttgag tagtgatag tgaagatga 
      tgcaattag ggacattta tgggattcc caaagactt 
      aaacattgt gtttgtcga tattgaaac ggcaaagaa 
      agaactaaa tagcaaagg attcgtttt aaagcattt 
      ggaacaaga cggagaggt cgagagaat tgtttgcac 
      ttgtgatgg agcatctca gtgcttata tcatatcaa 
      aatagaaga gccattaaa cattaaaga agaagagcc 
      agaaattcc caaaataga agttgaaac aacaagctt 
      aaatgaact taaaactaa aatgaaact taaacttaa 
      aaatgaacc agaagaaaa aaaatttga aacaattgc 
      ctccacctc taggatatc ttctccaaa attaaataa 
      ttcaaatat caaagatag agaacaaaa aaatccgaa 
      accaaagcg aaaacaata caagtgtaa gatttgtga 
      caaaatatt aaacttatc aggaaagta tcatcatct 
      tcgtaccca caaaaaacc gtttgtttg tgaattctg 
      tggtttgag attttcact aaaaggtag tctaaaggc 
      acaccttct acaacatac aggagaaaa accgcacgt 
      ttgtgaatt ctgtggtcg cagtttttc acgaaaaga 
      ctatctaac ggaacacct acatcgcca tacaggaaa 
      aaaaccaat tgtgtgtga acattgtgg taaaagttt 
      cagaactaa gccagtttt aaaaacaca tctacttgt 
      acacaatat agggtacgg ttgtgaatt ttgttcgaa 
      agttttccg atatgagaa atcacttaa ttcacacaa 
      acaaaatat acattcgga ggaaaaacc gtttgtttg 
      tacacattg taaccaaag atattgcac cttattggc 
      atttacaaa tcatctgga aactcatgg tattaaacg 
      taaaatcca cgaaagtgg cccattgtt atgtcaaat 
      ttgtgataa agaattttt gacaaaaaa cagattaaa 
      atcacactt gttgacaca cggagacca aaagttttc 
      ctgtagctt ttgcgctaa ggcatttaa cactaaagc 
      tcgacttat gatgcacaa cagagtcca tacaggaga 
      aaaacaaaa cgtttgtaa gtcttgtgg acgaacatt 
      caactctgt gccgggact ccaacatca tatgtattt 
      ccacactgg ggaaaaacc gtacgaatg taaaatgtg 
      cgataaagc ttatgtcct ggctgaaaa tttaaaata 
      tcatatgat ggttcatac tggtgaaaa accatattc 
      gtgtccaca ttgtgataa aaaatctag gagcaaatt 
      tgacatgga atgccacat taggattca tacggggga 
      aaaaccgtt caaatgtga cacctgttc gaaagcgtt 
      ccggaaccc gagatatct caaaagggg ctgccttgt 
      cttccatac tccaggata taatcaagt caagatggc 
      catattgag acaattatt aaccaaagc aatgaagcc 
      agaaatatt gcaagcccc cgaaattgg aaaaaccga 
      ttttgacaa tttgtgtct cgcttgttt atccaaaga 
      ttgtctcag gaatgtttt tgtgataac atacgaggg 
      ggctgcctt aatatcact actcgagga tttactcaa 
      ctttcacgt cgcagcagg cgatgactt gcctaaaaa 
      aatctgcaa aaaatgcgt cgaagaaat catccagtt 
      ttacgtctt taaaaaacg atttaacga aactcaaac 
      aaagctaca aagagtttt gatagaaaa ggatgataa 
      agtagtttc atctaatgg acaattttc acgtgatga 
      agaatttat tgttaaaga cagtgatgt ggaaaatga 
      agtttttgt gttgagtag tgatactga agatgatgc 
      acttaggga cttttatgg gattcccaa agacttaga 
      cattgtgtt tgtcgatat tgaaacggc aaagaagga 
      attaaatag cgaaggatt tgttttaaa gcatttgga 
      acaagatgg agaagttaa gacaattgt ttgtacctg 
      tgatggagc atctcagtg cttatatca tttgaaagt 
      aaaagaaca agaaatttc caaagtaga agttgaaac 
      agttgttcc cattctaga cttaaatca ttcaagcat 
      taaagataa accagaaga aaaagaagt tgaaacaat 
      tgtctccac ctctaggaa atcttctcc aaacttaaa 
      taattcaaa cagtgaaga taaagagca agaaattcc 
      ggaacaatt caagtgtaa catttgtga caaaatatt 
      caatttaag agtaaagaa gcaacatct ttgtacaca 
      taacataaa agagaaacc tcccacttg tcaaatttg 
      ttctcgtgt atttcgttt tagaaagca tttaaggga 
      acatatgag agtacacac gggggaaaa accgtttgt 
      ttgtgaatt ctgtggttt gagatttac acaaaatcg 
      tagtctaaa gtcacactt tctactaca tacaggaga 
      aaagccgta cgtttgtga attctgtgg tcgcagttt 
      ttcacgaaa ggactatct aactgaaca cctacatcg 
      ccatacagg aaaaaaaca atatgtgtg tgaatgttg 
      cggaaacgg tttcataac taatccaat gttaaaaaa 
      gcatctact tgtacacaa tatagggta cgtttgtga 
      attttgttc gaaagcttt ccgatatga gaaatcact 
      taattcaca caaacaaaa tagacattc ggaggaaaa 
      accgtttac ttgtacaca ctgtaacaa tagattttg 
      caccatatt ggcacttac aaaccatct gaaaactca 
      tggtattca acgtgaaat ccagcaaag tgagaactt 
      gtgtcaaat ttgtgataa ggaattttt ctcaaaaca 
      cagattaaa atcacactt gttgaatca cggaaacca 
      aaagttcat aactcgcgg aaaccaaaa gttgtcaac 
      tcgcggaaa ccaaaagtt gttgattca cggagacca 
      gaagttttc ctgtacctc ttgcgataa gaagtttta 
      cactcaagc tcaactcac gatacataa tagagtcca 
      tacaggaga aaaagaaaa cctttgtaa atattgtgg 
      tcaaatatt caccagtgg gaatggact tctacagca 
      tatgtatct ccatactgg cgaaaaacc atacgaatg 
      taaaatttg cgataaagc ttttgtcca gagtcatca 
      tttaaaaca tcatatgat ggttcatac tgatgaaaa 
      accctatgc gtgcacaca ttgtgacaa acaatttag 
      aagcaaatt taacttgga atgtcatgt caggcttca 
      tacggggga aaaaccgtt taaatgtga cacctgttc 
      caaagcgtt ccgaaaccc gatttatct cggaaggca 
      taagaagaa ggcacatta agacaattt tgcataaaa 
      taaaaatgt attgtcttg
    • >TC030971 RB
      ttatttctc caataaacc tcaaaaaaa acgtaaaac 
      tattaattc aaagttgca ctctcaaga ttcgaaaaa 
      ctgacattg tctactttt acttctcca atttatata 
      gctagagat tgtctcgtg tgcgttttt gtgataaat 
      ttcaggggg gctgacttg tcttccata ctccaggat 
      acaaacatt attcaagtc gctgttgat gcgttgccg 
      aagaaaatg tgtgaagtc gtccagttt tacatcttc 
      aagtagcga tttcaacac tacaaagtg tttaaccga 
      aatggataa taaaatagt ttcatgcaa cgaagaata 
      tgaatattc aagtgatga agaaagtga agttgaaga 
      tgaaccttt tgtgttgag tagtgatag tgaagatga 
      tgcaattag ggacattta tgggattcc caaagactt 
      aaacattgt gtttgtcga tattgaaac ggcaaagaa 
      agaactaaa tagcaaagg attcgtttt aaagcattt 
      ggaacaaga cggagaggt cgagagaat tgtttgcac 
      ttgtgatgg agcatctca gtgcttata tcatatcaa 
      aatagaaga gccattaaa cattaaaga agaagagcc 
      agaaattcc caaaataga agttgaaac aacaagctt 
      aaatgaact taaaactaa aatgaaact taaacttaa 
      aaatgaacc agaagaaaa aaaatttga aacaattgc 
      ctccacctc taggatatc ttctccaaa attaaataa 
      ttcaaatat caaagatag agaacaaaa aaatccgaa 
      accaaagcg aaaacaata caagtgtaa gatttgtga 
      caaaatatt aaacttatc aggaaagta tcatcatct 
      tcgtaccca caaaaaacc gtttgtttg tgaattctg 
      tggtttgag attttcact aaaaggtag tctaaaggc 
      acaccttct acaacatac aggagaaaa accgcacgt 
      ttgtgaatt ctgtggtcg cagtttttc acgaaaaga 
      ctatctaac ggaacacct acatcgcca tacaggaaa 
      aaaaccaat tgtgtgtga acattgtgg taaaagttt 
      cagaactaa gccagtttt aaaaacaca tctacttgt 
      acacaatat agggtacgg ttgtgaatt ttgttcgaa 
      agttttccg atatgagaa atcacttaa ttcacacaa 
      acaaaatat acattcgga ggaaaaacc gtttgtttg 
      tacacattg taaccaaag atattgcac cttattggc 
      atttacaaa tcatctgga aactcatgg tattaaacg 
      taaaatcca cgaaagtgg cccattgtt atgtcaaat 
      ttgtgataa agaattttt gacaaaaaa cagattaaa 
      atcacactt gttgacaca cggagacca aaagttttc 
      ctgtagctt ttgcgctaa ggcatttaa cactaaagc 
      tcgacttat gatgcacaa cagagtcca tacaggaga 
      aaaacaaaa cgtttgtaa gtcttgtgg acgaacatt 
      caactctgt gccgggact ccaacatca tatgtattt 
      ccacactgg ggaaaaacc gtacgaatg taaaatgtg 
      cgataaagc ttatgtcct ggctgaaaa tttaaaata 
      tcatatgat ggttcatac tggtgaaaa accatattc 
      gtgtccaca ttgtgataa aaaatctag gagcaaatt 
      tgacatgga atgccacat taggattca tacggggga 
      aaaaccgtt caaatgtga cacctgttc gaaagcgtt 
      ccggaaccc gagatatct caaaagggg ctgccttgt 
      cttccatac tccaggata taatcaagt caagatggc 
      catattgag acaattatt aaccaaagc aatgaagcc 
      agaaatatt gcaagcccc cgaaattgg aaaaaccga 
      ttttgacaa tttgtgtct cgcttgttt atccaaaga 
      ttgtctcag gaatgtttt tgtgataac atacgaggg 
      ggctgcctt aatatcact actcgagga tttactcaa 
      ctttcacgt aagtaactg ccatttcag gaaagtttt 
      actttagcc acactaaac gtatctgct taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY