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Gene information
General information
TC006833 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450512.1: 6815..8299
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006833
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04221

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006833 RA
      MNRVERREL KHFLTICNP TLIICTTKS LTNVQKLAN 
      ELELPQILL FSDLNNTPN NYNEELTPI DLDPTKEVA 
      LILTSSGTT GFPKCVQLT HLNLRTTML YAMDPNFLD 
      LNQNESTIA FLPYFHVFG CAVSLASIL SGCKSIVME 
      KFIPDLFLA NIQKHKVTK LFVVPPILQ FLVKNPMVG 
      KFDISSVVD ILCGAAVVG KELEEMVQE RFKVKSVRQ 
      VYGMTELCG AATMIPKNF QKYGSSGKV ISCTQIKVC 
      EVASGKTLA AQEIGEIRV KGDGTMKSY LKNEEETKK 
      AFDEEGFLK TGDLGYYDE EGYFYIVDR LKEIIKYKG 
      FQVSPAELE NLLIQHPAV KDAAVVGLP DERAGELPL 
      AFVVKQDQN VTEKELIRF ISENVSVQK HLYGGVRFI 
      ENIPKNSSG KILRLKLQE LL
    CDS sequence
    • >TC006833 RA
      atgaatcgg gtcgaacgc cgagaactg aaacacttc 
      ctaacaatt tgtaaccca actttgatc atttgcaca 
      accaaatcg ttaaccaac gtccaaaaa ctagcaaac 
      gagctcgaa ctcccccaa attttgctc ttttctgat 
      ctaaacaac acaccaaat aattacaac gaggaatta 
      accccaatt gacttggac cccaccaaa gaagttgcc 
      ctcatttta acatcttct ggtaccaca ggcttccca 
      aaatgcgtc caactaacc catttaaac ctacgcaca 
      acaatgtta tatgccatg gacccaaac ttcctcgac 
      ttgaaccaa aacgaatca accatcgct tttttacca 
      tatttccac gtttttggt tgtgctgtc tctcttgct 
      tcgattcta agtggttgt aagtcaata gttatggaa 
      aaatttatc cccgatttg tttttggcc aacatacag 
      aagcacaaa gttaccaaa ttgtttgtg gttcctccg 
      attttgcaa tttttggtt aaaaatcca atggttggg 
      aaatttgat atttcctca gttgttgat attttgtgt 
      ggggctgct gttgttggt aaagaattg gaagaaatg 
      gttcaggaa cggttcaag gtcaagtca gttaggcaa 
      gtttatggt atgacagaa ttatgtggt gctgcgacc 
      atgattccc aaaaatttt caaaaatat gggtcttct 
      gggaaagtg atttcctgt acccagatc aaagtttgt 
      gaggttgca agtggaaaa acactggca gctcaagaa 
      attggggaa atacgggtt aaaggcgat ggaaccatg 
      aagagttat ttgaagaat gaagaagaa acgaagaaa 
      gcgtttgat gaagaaggg tttttgaag actggtgat 
      ttgggttat tatgatgaa gaaggttat ttctacatt 
      gttgaccgt ttgaaggaa attatcaaa tataaagga 
      tttcaggta tcaccagct gagttggag aatttgttg 
      atacaacat cctgctgtt aaagatgca gctgttgtt 
      ggacttcct gatgaaaga gccggagag ttacctttg 
      gcatttgtt gttaaacag gatcaaaat gtgacggaa 
      aaagaattg attcgattc atttcagag aatgtttct 
      gtccagaaa catttgtat ggtggagtt agatttatt 
      gaaaacatt ccaaaaaat tccagtggg aaaattcta 
      agactaaaa ctacaagag ctgctatga
    mRNA sequence
    • >TC006833 RA
      gtttaaatg aatcacatg aatcgggtc gaacgccga 
      gaactgaaa cacttccta acaatttgt aacccaact 
      ttgatcatt tgcacaacc aaatcgtta accaacgtc 
      caaaaacta gcaaacgag ctcgaactc ccccaaatt 
      ttgctcttt tctgatcta aacaacaca ccaaataat 
      tacaacgag gaattaacc ccaattgac ttggacccc 
      accaaagaa gttgccctc attttaaca tcttctggt 
      accacaggc ttcccaaaa tgcgtccaa ctaacccat 
      ttaaaccta cgcacaaca atgttatat gccatggac 
      ccaaacttc ctcgacttg aaccaaaac gaatcaacc 
      atcgctttt ttaccatat ttccacgtt tttggttgt 
      gctgtctct cttgcttcg attctaagt ggttgtaag 
      tcaatagtt atggaaaaa tttatcccc gatttgttt 
      ttggccaac atacagaag cacaaagtt accaaattg 
      tttgtggtt cctccgatt ttgcaattt ttggttaaa 
      aatccaatg gttgggaaa tttgatatt tcctcagtt 
      gttgatatt ttgtgtggg gctgctgtt gttggtaaa 
      gaattggaa gaaatggtt caggaacgg ttcaaggtc 
      aagtcagtt aggcaagtt tatggtatg acagaatta 
      tgtggtgct gcgaccatg attcccaaa aattttcaa 
      aaatatggg tcttctggg aaagtgatt tcctgtacc 
      cagatcaaa gtttgtgag gttgcaagt ggaaaaaca 
      ctggcagct caagaaatt ggggaaata cgggttaaa 
      ggcgatgga accatgaag agttatttg aagaatgaa 
      gaagaaacg aagaaagcg tttgatgaa gaagggttt 
      ttgaagact ggtgatttg ggttattat gatgaagaa 
      ggttatttc tacattgtt gaccgtttg aaggaaatt 
      atcaaatat aaaggattt caggtatca ccagctgag 
      ttggagaat ttgttgata caacatcct gctgttaaa 
      gatgcagct gttgttgga cttcctgat gaaagagcc 
      ggagagtta cctttggca tttgttgtt aaacaggat 
      caaaatgtg acggaaaaa gaattgatt cgattcatt 
      tcagagaat gtttctgtc cagaaacat ttgtatggt 
      ggagttaga tttattgaa aacattcca aaaaattcc 
      agtgggaaa attctaaga ctaaaacta caagagctg 
      ctatgacgt ggattatta ataaatatt ccttatatg 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY