Status
Please wait ...
Gene information
General information
TC006810 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14525535..14530202
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006810
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt not completely molted - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006810 RA
      MSRLSQFYT VEVGDTKFT ILKRYQNLK PIGSGAQGI 
      VCAAVDTET QQNVAIKKL SRPFQNVTH AKRAYREFK 
      LMKLVNHKN IIGLLNAFT PQRSLEDFQ DVYLVMELM 
      DANLCQVIQ MDLDHERMS YLLYQMLCG IKHLHSAGI 
      IHRDLKPSN IVVKSDCTL KILDFGLAR TAGTTFMMT 
      PYVVTRYYR APEVILGMG YTENVDIWS VGCIMGEMI 
      RGGVLFPGT DHIDQWNKI IEQLGTPSQ QFMMRLQPT 
      VRNYVENRP RFTGFPFDK LFPDVLFPS DSSEHNRLK 
      ASQARDLLS RMLVIDPER RISVDDALL HPYINVWYD 
      ELEVNAPAP GPYDHSVDE REHTVEQWK ELIYQEVME 
      YESSRGRGA SSSQPSPAS NSIEPNGTD SMR
    CDS sequence
    • >TC006810 RA
      atgtcccgt ctgtctcaa ttctacacc gttgaggtg 
      ggcgacacc aaattcacc attctgaag aggtaccaa 
      aatctgaaa ccgatcggc tcaggagca caaggaatc 
      gtctgcgcc gctgttgat actgaaaca caacaaaat 
      gtagcgatt aagaaattg agtaggccc tttcaaaat 
      gtgacgcac gctaaaagg gcgtatcgc gaattcaag 
      ctcatgaaa ttagttaat cataaaaat attatcgga 
      cttttgaac gctttcacg cctcaaagg tccttagaa 
      gacttccaa gatgtttat ttagtgatg gaattaatg 
      gacgcgaat ctatgtcaa gtcatacaa atggattta 
      gatcatgaa aggatgagt tatctcctt tatcaaatg 
      ctttgcggt attaaacat ttacattca gctggtatt 
      atacatagg gatctcaaa ccaagcaat attgtagtt 
      aaatcagat tgtacattg aaaatttta gactttggt 
      cttgcacgt actgctggt actactttt atgatgact 
      ccatacgtg gtcactaga tattacaga gcgccagaa 
      gttatctta ggtatggga tatacggaa aatgtggac 
      atttggagc gtcggatgt atcatgggt gagatgata 
      agaggagga gtcttattt cctggtact gatcacata 
      gaccaatgg aataaaatc atagaacaa ctgggtaca 
      ccatcgcaa caattcatg atgcgtttg caaccaact 
      gttcgcaac tacgtcgaa aatcggccc cgtttcacg 
      ggcttcccc ttcgacaag ctcttcccc gatgtgctg 
      tttccctcc gacagcagc gaacacaat cgactgaaa 
      gcgagtcaa gcgagagat ttactatcc aggatgctt 
      gtaatcgat ccagaacgc aggatatcg gtcgacgat 
      gcacttctt catccatac atcaacgtc tggtatgac 
      gaactagaa gtgaatgcg cccgcacca ggaccctac 
      gatcatagt gtcgatgaa agagaacac actgtcgaa 
      caatggaag gaacttatc taccaagaa gttatggaa 
      tatgaaagt tcgcgcgga agaggcgcc tccagttca 
      caaccgagt cccgcatct aattctatt gagccaaat 
      gggacggat tcaatgcgg tag
    mRNA sequence
    • >TC006810 RA
      attgtttca gagcttcaa ttctaactt ggcaccgtc 
      ctaccaata gagccacta aaatggatc gttcttgac 
      gacggcgtc gaactattg ggactggcg ctataaaat 
      gtcccgtct gtctcaatt ctacaccgt tgaggtggg 
      cgacaccaa attcaccat tctgaagag gtaccaaaa 
      tctgaaacc gatcggctc aggagcaca aggaatcgt 
      ctgcgccgc tgttgatac tgaaacaca acaaaatgt 
      agcgattaa gaaattgag taggccctt tcaaaatgt 
      gacgcacgc taaaagggc gtatcgcga attcaagct 
      catgaaatt agttaatca taaaaatat tatcggact 
      tttgaacgc tttcacgcc tcaaaggtc cttagaaga 
      cttccaaga tgtttattt agtgatgga attaatgga 
      cgcgaatct atgtcaagt catacaaat ggatttaga 
      tcatgaaag gatgagtta tctccttta tcaaatgct 
      ttgcggtat taaacattt acattcagc tggtattat 
      acataggga tctcaaacc aagcaatat tgtagttaa 
      atcagattg tacattgaa aattttaga ctttggtct 
      tgcacgtac tgctggtac tacttttat gatgactcc 
      atacgtggt cactagata ttacagagc gccagaagt 
      tatcttagg tatgggata tacggaaaa tgtggacat 
      ttggagcgt cggatgtat catgggtga gatgataag 
      aggaggagt cttatttcc tggtactga tcacataga 
      ccaatggaa taaaatcat agaacaact gggtacacc 
      atcgcaaca attcatgat gcgtttgca accaactgt 
      tcgcaacta cgtcgaaaa tcggccccg tttcacggg 
      cttcccctt cgacaagct cttccccga tgtgctgtt 
      tccctccga cagcagcga acacaatcg actgaaagc 
      gagtcaagc gagagattt actatccag gatgcttgt 
      aatcgatcc agaacgcag gatatcggt cgacgatgc 
      acttcttca tccatacat caacgtctg gtatgacga 
      actagaagt gaatgcgcc cgcaccagg accctacga 
      tcatagtgt cgatgaaag agaacacac tgtcgaaca 
      atggaagga acttatcta ccaagaagt tatggaata 
      tgaaagttc gcgcggaag aggcgcctc cagttcaca 
      accgagtcc cgcatctaa ttctattga gccaaatgg 
      gacggattc aatgcggta gaaaaggtg agtgttatc 
      attgactga agagcgaca aaattaaaa aaatttaat 
      tattaaaat taaacacaa accataaaa aaagttttg 
      tttgcttca ccgttaaca aaggatgaa tttattcca 
      acaaaaata aagcatagc tttgtgaaa aagttttgc 
      tttaaattc acatagtcc atgaaaagt caaacacat 
      ccagagtat tacataatc gaatcatat ttaaaaatt 
      aaatggatt tggaaacgt ttaatgaat tttttctcc 
      gtttatgtg tttagccga cagcgtctt cttagttgt 
      tttttaata tttctttat tgtattcta gacaagtgt 
      aataataac actaacaaa aagctcttt acgtccaac 
      ttttttaac agcttgttc tctttcgtg tcgtcattg 
      tggtattta ttgtagttt ttgttttag gatttgaga 
      tataaagca caagctgca atttaagct agtctaaac 
      tattattga aaatattac tgatataaa gcccaccat 
      taactagat accgaacag acagagagt ctagtgagc 
      tgtgtttgt tattgacga cagtgagta taagactac 
      acacgggtc ccttttttc tatcaaccg gaacattat 
      aatatatcc ttaatcagt atgtgtgat atgataact 
      taagactaa tttgtattt gaatcgttt tgtttttcc 
      aaacttgtt tttttaacg aaataaata acgtatttt 
      tccaagata cagatatac gaagcattg tttgaaaga 
      acgtcgttt ttagacaat cattcagtt cgttcggtt 
      tccattgaa aaatacaaa ttagtaatg aatgtataa 
      caaaataat caatattag gtagtacct agttattat 
      tgattatta tcggtagtt ttgtttttt taatattat 
      ttatttttt cttctgtca aatgcaata ttttttcta 
      acgttaggt ataacttat ttttgctct ttttcaaat 
      tttatagtt atatataga cataaatag aattagcga 
      ctataacca tttgaaatt aaggtagga acatcaaat 
      tgtttacat aatttgcaa acaaattgt gtgaaacta 
      gcgaaaaac aattacaat aactttatg tacttgtga 
      acatccgat ccaaaaaag aatgtaaaa agtgacaat 
      tagtgaatt ttataggtg gacttttta ttgttgatt 
      accattttg ttgtgtttt ttggacgat aagtttctt 
      tatttattc gtgcaatgt atgaacgtc actggcgtt 
      ttcattttt gttttatta ttaattttt ttattgtga 
      ttaaatcgt gaatgtgca aagactttt aaaggaaaa 
      aaaattaac tcaagtcga gttgtgtag tggtttgtt 
      taatttatt tttattttt tacataata atttattaa 
      gatggcagt tttttcctt tctctcttt cataaaaat 
      actgtaaat accttctca attagactt acttaattt 
      ttcgacttt ccacttatc cttaaataa aaaacaaaa 
      cgttttaag cgtagacaa caaattgca aatcggtag 
      aatgttttc gtggttttc gtgttggtg atgttggga 
      tcacctttt attcagttc ttgttcttt ttcgaataa 
      cacagacta gtgttttac cggtttaca acaggatta 
      ttatatcac gtttagtaa tttgtagta tacatacaa 
      tgcttgtat tgttctgct ttgtaaata aacaaagta 
      aactgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY