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Gene information
General information
TC006809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14514615..14519838
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006809
    iBeetle screen iB_06397

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages potentially structure fused -
    mesothorax circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006809 RA
      MKCGTILLI ISTLIGIYI GILTRRVSN LFDPSKCPF 
      CYGSDLCKD IARIRLQYK TFSDLFYNL FSVKNIFFA 
      EYDGKRVVL KKLGHDSEL EQIKSHSVD EESLVDYLV 
      NSASGLRIC YRDVAKRLV KRLRKHFDL EQIWTISQV 
      NCEPLMLAI LNGPIPKLY GSCGFVIVE EYCGQPLNH 
      FETSPWHVR AFLALQLLE AAIDFTHKH HDFRVYLTD 
      ISPDNIAVD HNFKLTFVD LEHVILQRK ENQTDVVHN 
      TQFFPDDDF IFSEAEICS NSLSDHNIY AVCRLLLSR 
      KAPWPMMAN GLLHSPPPT IARYNNELF LLLEDCVDS 
      NQRFDIAEK LINLLKKLN KDIS
    CDS sequence
    • >TC006809 RA
      atgaaatgt ggtacgatc ctgttaata atttccaca 
      ctcatcgga atttacatc gggattttg actcgaaga 
      gtttcaaat ctgtttgat ccgagcaaa tgtccgttc 
      tgttacggc agtgatcta tgcaaggat atcgccagg 
      atcaggctc caatataaa actttcagc gacttgttt 
      tataattta ttcagcgtg aaaaatatt ttttttgct 
      gaatatgat ggaaaaagg gtcgttttg aaaaagttg 
      ggccatgac agtgaactt gaacaaatc aaatcacac 
      tctgttgac gaagaatcg ctcgttgat tacttagtt 
      aacagtgcc agtggtttg agaatttgt tatcgagat 
      gttgcgaaa cgtttagtc aaaagattg aggaaacat 
      ttcgacttg gagcaaatt tggacgatt agtcaagtc 
      aattgcgag cctttgatg ttggcaatt ttaaacgga 
      ccgattcca aaattatat gggagttgt ggttttgta 
      attgtcgaa gagtattgt ggtcaacca ctcaatcat 
      tttgaaacg tcaccatgg cacgtgcga gctttttta 
      gctttgcaa ttgttggaa gctgctatt gattttaca 
      cacaaacat cacgatttc agggtgtat ttgacagat 
      atatcacct gataatatc gcagtcgat cataatttc 
      aaactgact tttgtcgac ttggaacat gttattttg 
      caacgcaaa gaaaatcaa accgatgtt gttcataat 
      actcaattt ttccctgac gatgatttt atctttagt 
      gaagccgaa atctgttca aactcttta agtgaccac 
      aatatctat gccgtgtgc cgattgttg ttgagtcgt 
      aaagctcct tggcccatg atggcaaat ggcctcctt 
      cattcaccg cctccgaca atcgctcga tataataac 
      gaacttttc ttactgctt gaagactgt gttgactca 
      aaccagcgc tttgatatc gctgagaaa cttataaat 
      ctgttaaaa aaactgaat aaagatata tcttga
    mRNA sequence
    • >TC006809 RA
      cttgcagtg aaaaagtgt actagtgtt ggtgtccta 
      acgcgtgcg cttttccaa cgaatttca cgaatccat 
      tgaagtctt cttttgact ttcttgatg atatgtggt 
      gtgtgttgt aaatttcct agtttactt catgttttg 
      tgttggaat taactttag ttcagtgtt ttgttcgat 
      ttggaagtg aagcgatta ccctgcacc acagctaca 
      caagatatg aaatagcag cactgttcc accaactac 
      aagactaaa tacgacatt aaggtaagg tgatttggc 
      tttgtgttc gaaaaaaaa aacgattaa ataacaaat 
      aagatcact tgtgctacc actgaattc gtgtctttt 
      tttctcaca agtcctaca ccaataacg ataaatacc 
      gttgtattt gcacaagac ttggggcaa gtcatatgt 
      ctatcaaga ttaatagtc gtcattaat tagaatgac 
      actacaaca gcggcgttt accaatgat taattacaa 
      ttttgataa cggagaggg aagccagat cggtctcaa 
      atcttagtg aatcgagtg aaaattaca gcaaagtgt 
      ataacaaat tggcaatca ttagcaaag ccaatattt 
      agagccgag tcaagtcac aaccagaat atatcatca 
      actgcccgc cctacgttt tcctatcat gtccgtgca 
      tcccttacc gagggtcaa acctctttg ttaaattta 
      ttggaacag atgttccga agaaattcg attaattag 
      tagattcta tcacctgtg agttgatta aactaagaa 
      gtttcttca acagaaatc aataggttg taatggcat 
      ttccgcttt ttagatgaa atgtggtac gatcctgtt 
      aataatttc cacactcat cggaattta catcgggat 
      tttgactcg aagagtttc aaatctgtt tgatccgag 
      caaatgtcc gttctgtta cggcagtga tctatgcaa 
      ggatatcgc caggatcag gctccaata taaaacttt 
      cagcgactt gttttataa tttattcag cgtgaaaaa 
      tattttttt tgctgaata tgatggaaa aagggtcgt 
      tttgaaaaa gttgggcca tgacagtga acttgaaca 
      aatcaaatc acactctgt tgacgaaga atcgctcgt 
      tgattactt agttaacag tgccagtgg tttgagaat 
      ttgttatcg agatgttgc gaaacgttt agtcaaaag 
      attgaggaa acatttcga cttggagca aatttggac 
      gattagtca agtcaattg cgagccttt gatgttggc 
      aattttaaa cggaccgat tccaaaatt atatgggag 
      ttgtggttt tgtaattgt cgaagagta ttgtggtca 
      accactcaa tcattttga aacgtcacc atggcacgt 
      gcgagcttt tttagcttt gcaattgtt ggaagctgc 
      tattgattt tacacacaa acatcacga tttcagggt 
      gtatttgac agatatatc acctgataa tatcgcagt 
      cgatcataa tttcaaact gacttttgt cgacttgga 
      acatgttat tttgcaacg caaagaaaa tcaaaccga 
      tgttgttca taatactca atttttccc tgacgatga 
      ttttatctt tagtgaagc cgaaatctg ttcaaactc 
      tttaagtga ccacaatat ctatgccgt gtgccgatt 
      gttgttgag tcgtaaagc tccttggcc catgatggc 
      aaatggcct ccttcattc accgcctcc gacaatcgc 
      tcgatataa taacgaact tttcttact gcttgaaga 
      ctgtgttga ctcaaacca gcgctttga tatcgctga 
      gaaacttat aaatctgtt aaaaaaact gaataaaga 
      tatatcttg agtcgcctt tattctagt taaattcgg 
      acctaaaag ggagattaa ttcggggtt ttgcgtcta 
      catacgtgt gtgtagacc taagccaag tgggatggt 
      acggttgta gtgtgataa attggtcgt gcaagattt 
      tggaagcga cttatattt agataatta cgctcgaag 
      atgattaga cgagacttt attttagga ttgaaaggg 
      atggaatta agtggaagg gtgggttta attaggcac 
      gtatgtgtt gacatggca atgaaacga gtggaacag 
      ctttatttg tgaccggcg ataaaaggg gatcttgtt 
      tgaagaagc taaataatg aaatcactt tcacatcag 
      ttcgaattt ttttatcaa agtttatgc aaagttgag 
      atttttttc aacagaaaa aattgaagg tttaataag 
      gggatacga tagtttaga aatcaaata catatttgc 
      acttacgag tacatattc caaaaataa acattttaa 
      aatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY