Status
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Gene information
General information
TC006809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14514615..14519838
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006809
    iBeetle screen iB_06397

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages potentially structure fused -
    mesothorax circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006809 RA
      MKCGTILLI ISTLIGIYI GILTRRVSN LFDPSKCPF 
CYGSDLCKD IARIRLQYK TFSDLFYNL FSVKNIFFA 
EYDGKRVVL KKLGHDSEL EQIKSHSVD EESLVDYLV 
NSASGLRIC YRDVAKRLV KRLRKHFDL EQIWTISQV 
NCEPLMLAI LNGPIPKLY GSCGFVIVE EYCGQPLNH 
FETSPWHVR AFLALQLLE AAIDFTHKH HDFRVYLTD 
ISPDNIAVD HNFKLTFVD LEHVILQRK ENQTDVVHN 
TQFFPDDDF IFSEAEICS NSLSDHNIY AVCRLLLSR 
KAPWPMMAN GLLHSPPPT IARYNNELF LLLEDCVDS 
NQRFDIAEK LINLLKKLN KDIS
    CDS sequence
    • >TC006809 RA
      atgaaatgt ggtacgatc ctgttaata atttccaca 
ctcatcgga atttacatc gggattttg actcgaaga 
gtttcaaat ctgtttgat ccgagcaaa tgtccgttc 
tgttacggc agtgatcta tgcaaggat atcgccagg 
atcaggctc caatataaa actttcagc gacttgttt 
tataattta ttcagcgtg aaaaatatt ttttttgct 
gaatatgat ggaaaaagg gtcgttttg aaaaagttg 
ggccatgac agtgaactt gaacaaatc aaatcacac 
tctgttgac gaagaatcg ctcgttgat tacttagtt 
aacagtgcc agtggtttg agaatttgt tatcgagat 
gttgcgaaa cgtttagtc aaaagattg aggaaacat 
ttcgacttg gagcaaatt tggacgatt agtcaagtc 
aattgcgag cctttgatg ttggcaatt ttaaacgga 
ccgattcca aaattatat gggagttgt ggttttgta 
attgtcgaa gagtattgt ggtcaacca ctcaatcat 
tttgaaacg tcaccatgg cacgtgcga gctttttta 
gctttgcaa ttgttggaa gctgctatt gattttaca 
cacaaacat cacgatttc agggtgtat ttgacagat 
atatcacct gataatatc gcagtcgat cataatttc 
aaactgact tttgtcgac ttggaacat gttattttg 
caacgcaaa gaaaatcaa accgatgtt gttcataat 
actcaattt ttccctgac gatgatttt atctttagt 
gaagccgaa atctgttca aactcttta agtgaccac 
aatatctat gccgtgtgc cgattgttg ttgagtcgt 
aaagctcct tggcccatg atggcaaat ggcctcctt 
cattcaccg cctccgaca atcgctcga tataataac 
gaacttttc ttactgctt gaagactgt gttgactca 
aaccagcgc tttgatatc gctgagaaa cttataaat 
ctgttaaaa aaactgaat aaagatata tcttga
    mRNA sequence
    • >TC006809 RA
      cttgcagtg aaaaagtgt actagtgtt ggtgtccta 
acgcgtgcg cttttccaa cgaatttca cgaatccat 
tgaagtctt cttttgact ttcttgatg atatgtggt 
gtgtgttgt aaatttcct agtttactt catgttttg 
tgttggaat taactttag ttcagtgtt ttgttcgat 
ttggaagtg aagcgatta ccctgcacc acagctaca 
caagatatg aaatagcag cactgttcc accaactac 
aagactaaa tacgacatt aaggtaagg tgatttggc 
tttgtgttc gaaaaaaaa aacgattaa ataacaaat 
aagatcact tgtgctacc actgaattc gtgtctttt 
tttctcaca agtcctaca ccaataacg ataaatacc 
gttgtattt gcacaagac ttggggcaa gtcatatgt 
ctatcaaga ttaatagtc gtcattaat tagaatgac 
actacaaca gcggcgttt accaatgat taattacaa 
ttttgataa cggagaggg aagccagat cggtctcaa 
atcttagtg aatcgagtg aaaattaca gcaaagtgt 
ataacaaat tggcaatca ttagcaaag ccaatattt 
agagccgag tcaagtcac aaccagaat atatcatca 
actgcccgc cctacgttt tcctatcat gtccgtgca 
tcccttacc gagggtcaa acctctttg ttaaattta 
ttggaacag atgttccga agaaattcg attaattag 
tagattcta tcacctgtg agttgatta aactaagaa 
gtttcttca acagaaatc aataggttg taatggcat 
ttccgcttt ttagatgaa atgtggtac gatcctgtt 
aataatttc cacactcat cggaattta catcgggat 
tttgactcg aagagtttc aaatctgtt tgatccgag 
caaatgtcc gttctgtta cggcagtga tctatgcaa 
ggatatcgc caggatcag gctccaata taaaacttt 
cagcgactt gttttataa tttattcag cgtgaaaaa 
tattttttt tgctgaata tgatggaaa aagggtcgt 
tttgaaaaa gttgggcca tgacagtga acttgaaca 
aatcaaatc acactctgt tgacgaaga atcgctcgt 
tgattactt agttaacag tgccagtgg tttgagaat 
ttgttatcg agatgttgc gaaacgttt agtcaaaag 
attgaggaa acatttcga cttggagca aatttggac 
gattagtca agtcaattg cgagccttt gatgttggc 
aattttaaa cggaccgat tccaaaatt atatgggag 
ttgtggttt tgtaattgt cgaagagta ttgtggtca 
accactcaa tcattttga aacgtcacc atggcacgt 
gcgagcttt tttagcttt gcaattgtt ggaagctgc 
tattgattt tacacacaa acatcacga tttcagggt 
gtatttgac agatatatc acctgataa tatcgcagt 
cgatcataa tttcaaact gacttttgt cgacttgga 
acatgttat tttgcaacg caaagaaaa tcaaaccga 
tgttgttca taatactca atttttccc tgacgatga 
ttttatctt tagtgaagc cgaaatctg ttcaaactc 
tttaagtga ccacaatat ctatgccgt gtgccgatt 
gttgttgag tcgtaaagc tccttggcc catgatggc 
aaatggcct ccttcattc accgcctcc gacaatcgc 
tcgatataa taacgaact tttcttact gcttgaaga 
ctgtgttga ctcaaacca gcgctttga tatcgctga 
gaaacttat aaatctgtt aaaaaaact gaataaaga 
tatatcttg agtcgcctt tattctagt taaattcgg 
acctaaaag ggagattaa ttcggggtt ttgcgtcta 
catacgtgt gtgtagacc taagccaag tgggatggt 
acggttgta gtgtgataa attggtcgt gcaagattt 
tggaagcga cttatattt agataatta cgctcgaag 
atgattaga cgagacttt attttagga ttgaaaggg 
atggaatta agtggaagg gtgggttta attaggcac 
gtatgtgtt gacatggca atgaaacga gtggaacag 
ctttatttg tgaccggcg ataaaaggg gatcttgtt 
tgaagaagc taaataatg aaatcactt tcacatcag 
ttcgaattt ttttatcaa agtttatgc aaagttgag 
atttttttc aacagaaaa aattgaagg tttaataag 
gggatacga tagtttaga aatcaaata catatttgc 
acttacgag tacatattc caaaaataa acattttaa 
aatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY