Status
Please wait ...
Gene information
General information
TC006802 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14389344..14392684
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006802
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04216

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: the leg musculature seems to be normal
    abdomen musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen musculature sometimes rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen musculature partially thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006802 RA
      MEANKRQVE MKLNHECVY VFETKGEMV PSKFRRKVW 
      QILPSICAT LMCLPFGIM LGWPSPTYP TLVQPGAPV 
      WISMDQSAM VAGFLMIGN TVSTPFSTI DRIGAKYGI 
      IIGASLITI GWILMWQAR DIFWLLGSR FLIGAGNGF 
      GTGQVKLYI TEICQDSLA QTMAKQINL YVFLGVVMA 
      FSYGPFVDF RNFSIINSI LSVLVLFLA IFLPSTPRE 
      LVKSGKMTE ARKLIAFLS PDLSVSEEI VKIKERINA 
      PDCSLGFCD LVRDKSLRR SLVVFVGLV FFGQFSGAI 
      ATLVYSQMI FERTHCPRP EMCALAYAL IFLVANFYG 
      TFCVPSHNK KYVLLFSSI GVSVLLVLN IVVLVEELN 
      VQFWQFTSL VGLLLYILV YTVGLGTVP VLLIPELFP 
      KEANKVVVQ FFVMSNSML ALTITKIFQ VLFTNMGLV 
      TPLCLFLTA STFSIIFVI IFVPNKSNN KSNNT
    CDS sequence
    • >TC006802 RA
      atggaagct aataaaaga caagtggag atgaagctc 
      aaccatgag tgcgtttac gtcttcgaa acgaaagga 
      gagatggtc ccttcgaaa tttcggcgg aaagtctgg 
      caaatccta ccatcgatc tgtgcaaca cttatgtgc 
      ctccccttt ggcataatg ttagggtgg ccttcccca 
      acttatccc actttggtc caacctggg gccccagtc 
      tggatcagt atggaccaa agtgccatg gtggcaggc 
      ttcctcatg attggcaat actgtctcc acccctttt 
      tccacaatt gaccgaatt ggagcaaaa tacgggatc 
      ataatcggc gccagcctc atcaccatt ggctggatc 
      ctgatgtgg caagcccgc gatattttc tggctcctg 
      gggagccgc ttcctgatt ggtgcagga aatggtttc 
      ggcaccggt caagtcaaa ctctacatc accgaaatc 
      tgccaagac tctctcgcc caaaccatg gccaaacaa 
      atcaacctc tatgttttc ctaggagtg gttatggcc 
      ttctcttac ggaccgttt gtcgatttc cgaaatttc 
      agcatcatt aactcaatc ctctcagtt ctggtcctt 
      ttccttgcc attttcttg ccgtcaact ccacgcgaa 
      ttggtcaag agtggtaaa atgaccgag gcgaggaaa 
      ttgatagcg tttctgagt cctgatttg agcgttagc 
      gaggaaatt gtgaagatt aaggagcgg atcaatgct 
      cctgattgc agtctgggg ttttgtgac ttagttagg 
      gacaagagt ttgaggagg agtttggtg gtttttgtt 
      ggactggtg ttttttgga cagttttcg ggagctata 
      gccacattg gtttactcg caaatgatt tttgagagg 
      acacattgt ccgaggccc gaaatgtgt gccttggcc 
      tatgctctt attttccta gtggcgaat ttttacggg 
      actttttgt gtcccgagt cataacaaa aagtacgtt 
      ctcttgttc tcctcaatt ggagtgtca gtattacta 
      gtgttaaat attgtagtg ttagttgag gaactaaac 
      gtccagttt tggcaattc acctctttg gtgggcctg 
      ctattgtac attctagtc tatactgtg ggattagga 
      accgttcct gtcctcctg atccctgaa ttgttcccc 
      aaggaagca aacaaagtc gtagtccaa tttttcgtc 
      atgtctaac tcaatgcta gctttgacc ataactaaa 
      atcttccag gttttattc accaatatg gggctggtc 
      acccctttg tgcttattt ctgacagct agcaccttc 
      tctatcatc tttgtgata atttttgtg cccaataaa 
      agtaacaat aaatcgaat aacacgtga
    mRNA sequence
    • >TC006802 RA
      gagtagtag tttaagaat tttatcaaa aaattcagt 
      gattaatta gcaaatttt ggctcgctt caattcctt 
      tatcagaca tcacacgtg ttattaaca cagaaatat 
      cattttgtc tgtaaataa taacaaatt aagaccaca 
      ggttgaatt aattaatgg gcagattgc atgtagtgt 
      taaataaac aaagggttt attattaca tattgtata 
      attatgtgg tgtagcagt gccactata tgactggag 
      aggcgtgaa aattattgc caattttgt gaaacaatc 
      agttgctga tctcaggta taaaacaat aaaagtgat 
      aaatcaaca aaaatattt agctaagtg cgccccaat 
      ggaagctaa taaaagaca agtggagat gaagctcaa 
      ccatgagtg cgtttacgt cttcgaaac gaaaggaga 
      gatggtccc ttcgaaatt tcggcggaa agtctggca 
      aatcctacc atcgatctg tgcaacact tatgtgcct 
      cccctttgg cataatgtt agggtggcc ttccccaac 
      ttatcccac tttggtcca acctggggc cccagtctg 
      gatcagtat ggaccaaag tgccatggt ggcaggctt 
      cctcatgat tggcaatac tgtctccac ccctttttc 
      cacaattga ccgaattgg agcaaaata cgggatcat 
      aatcggcgc cagcctcat caccattgg ctggatcct 
      gatgtggca agcccgcga tattttctg gctcctggg 
      gagccgctt cctgattgg tgcaggaaa tggtttcgg 
      caccggtca agtcaaact ctacatcac cgaaatctg 
      ccaagactc tctcgccca aaccatggc caaacaaat 
      caacctcta tgttttcct aggagtggt tatggcctt 
      ctcttacgg accgtttgt cgatttccg aaatttcag 
      catcattaa ctcaatcct ctcagttct ggtcctttt 
      ccttgccat tttcttgcc gtcaactcc acgcgaatt 
      ggtcaagag tggtaaaat gaccgaggc gaggaaatt 
      gatagcgtt tctgagtcc tgatttgag cgttagcga 
      ggaaattgt gaagattaa ggagcggat caatgctcc 
      tgattgcag tctggggtt ttgtgactt agttaggga 
      caagagttt gaggaggag tttggtggt ttttgttgg 
      actggtgtt ttttggaca gttttcggg agctatagc 
      cacattggt ttactcgca aatgatttt tgagaggac 
      acattgtcc gaggcccga aatgtgtgc cttggccta 
      tgctcttat tttcctagt ggcgaattt ttacgggac 
      tttttgtgt cccgagtca taacaaaaa gtacgttct 
      cttgttctc ctcaattgg agtgtcagt attactagt 
      gttaaatat tgtagtgtt agttgagga actaaacgt 
      ccagttttg gcaattcac ctctttggt gggcctgct 
      attgtacat tctagtcta tactgtggg attaggaac 
      cgttcctgt cctcctgat ccctgaatt gttccccaa 
      ggaagcaaa caaagtcgt agtccaatt tttcgtcat 
      gtctaactc aatgctagc tttgaccat aactaaaat 
      cttccaggt tttattcac caatatggg gctggtcac 
      ccctttgtg cttatttct gacagctag caccttctc 
      tatcatctt tgtgataat ttttgtgcc caataaaag 
      taacaataa atcgaataa cacgtgatt tcgtttatt 
      tcttcttac atcattaca attgactta attgattca 
      ctacttatt ctaagtttg tatcctcac gaagtagtc 
      tccgcaatt tttccttca tctctttgg acttttcaa 
      agttccgtt gacgtcgag ctagtcgtt cttctttca 
      gacatttgg tcatttgat ggtcgaggg aattgcaat 
      aaccgacga atttccgat agggatgat cattgtggc 
      attcttgca acaatcgga tgagttcat tgcactttt 
      ctgagtgca aatgtgtcg acaggtctg tgtgcattt 
      tactaagaa tgacaatcg agtgggctt gtgtcaaaa 
      ctacaccag acatctagc gcacaatac acaaactac 
      gatcaagta gaaaacttt aaatagagg gagccacac 
      aacagattt gaatttttt gaagcaaaa ataactcat 
      cgattttgt aaatttgcg tgcgttttg ggctgttta 
      aattgccaa acataagtt aaatgtctg atagtcgtt 
      tcaatgaaa cgtttattc gagtttaac atgcaatag 
      atgacttgt acacaataa gaacttaat taaagacta 
      taagtgcac gtgtctttt atttataat ggaagtgaa 
      cgttaatgg gatactaaa ttgatgata ttggtggtt 
      cgtggtctc aatttaatt tgcagattg agtataatg 
      gagttttgc aaaaagagg agcaaattt cgattgaat 
      cattcttaa aaaaatatt aaaataaat gagcttaat 
      ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY