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Gene information
General information
TC006801 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14382314..14385515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006801
    iBeetle screen iB_04215

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head bristle number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gena triplet potentially position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    vertex triplet vertex triplet ventral potentially position ventralized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    bell row anteroposterior-axis elongated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006801 RA
      MASVISLSS ASLNASPKN TDSPVTVLT LSSEIHRVP 
      VSWDGHKGA KRTSKVLDS DNTPNHYEK VKEWIDTTK 
      YDDLGPPNE NKSVCIRYD FDKSEKITI NVPKSCFTR 
      VTPLFSYME QAKKAALSD EKKSQECTA KVNGNLNFE 
      TPLSPESQK KELCSYLQL MKPGEKKQK KLLFGNRRS 
      KRVKNLVQN HQKTCSEND INTFPTLKS FEDLNIFES 
      KKKKETPNK YLPLPPKSK FSEFVDFED SVRKIVESV 
      KLRSKVTKR RKFNWGLKS HQFNFKRIN SLRSKGRLR 
      FISLDRLIL RKSRKRRKK FLKKDSPQK NACPHCDSV 
      EKQQRCEIV SVHTNSDDE NIDLKNFVD DMLELDKMS 
      EEHRKSVIE TRILASSSI TNLINKQMA QEADVQKAY 
      KIFADLSEK KTTEEKENK DLVTDFVLI EHDYAKIGE 
      LPSQVEEIN ELTHEESVK ISEPSTEED QESAKINEL 
      STEEEDEES VQNSLSPVP IPDLEVLPV NTDIEIKSL 
      HRSLTRTNR RKLFLNNYG LHNWNSGSH SDNRKLNHS 
      ISITKEDGA VLRGFYQDF NLVIVQEKS VSFWTQSAL 
      GNVLGAQNM WIPKGQIQR IPLNSTYVQ KDANEMVIS 
      TETSVAYVE LWTKEHKSD VREVPVADV FATVYFWRL 
      RQSGLEKKA LQLENIKGC AEDVRYCVL RCYPRIIVC 
      WQLVQGNEK RTKIHSFHL AADFQTVAN ISEIQPVEH 
      YVSSLHNID DCDSLIMGC GESKITLWN IEYGYIVSS 
      IELSNIKSP LATHWVKCD RGFLFMIQE CADYELRLI 
      AINGMNHSW KKLQNFDPP TGFEGLKGV CVENGIVIA 
      YYSQGILCW NAQSGEPIV EEVPNECEY IPSGKHVIL 
      LIDENVIVR NSLSHLITD L
    CDS sequence
    • >TC006801 RA
      atggcaagt gttatttcc ctctcgagt gcatcgttg 
      aacgcttcc cccaaaaac accgattct cctgtgact 
      gttctaacc ctaagttcg gaaatacac cgagttccc 
      gtgagttgg gacggccac aaaggtgcc aaaaggacg 
      tccaaggtc ttggactct gataacacc cccaatcat 
      tacgaaaaa gtcaaagaa tggatagat acgaccaag 
      tacgatgat ctggggccc cctaatgag aataaaagc 
      gtttgtatc cgctatgat ttcgataaa agcgagaaa 
      atcacaata aatgtgccg aaatcatgt tttacgcga 
      gtcactccc ttgttttct tatatggaa caggccaaa 
      aaagcggct ctgagtgac gagaaaaaa agccaggaa 
      tgtacagca aaagtcaat ggaaattta aactttgaa 
      actccccta agcccagaa agccagaaa aaggaactt 
      tgcagttac ttacagcta atgaaacct ggcgaaaag 
      aaacagaag aaactgtta ttcggaaac agacgatcg 
      aagcgagtc aagaattta gtccaaaac caccaaaaa 
      acgtgttca gaaaacgac ataaacacc ttccccacc 
      ctcaaatca ttcgaagat ttgaacatt tttgagtcg 
      aagaagaaa aaagaaacg cccaataaa tatttacca 
      ttgccccca aagtcaaaa tttagcgaa tttgttgat 
      tttgaggac tcggttcgt aagattgtc gaaagtgtc 
      aagttgagg agtaaagtg acaaagcgc cgaaaattt 
      aattggggg ctcaaatcg catcagttt aatttcaaa 
      cgaattaat tcactacgt agcaaaggt cggctcagg 
      ttcatttct ttggatcgg ctcattttg cgtaaaagt 
      cggaaacgg cggaaaaaa tttctcaag aaagattca 
      ccacaaaaa aatgcatgt ccccactgt gattcggtg 
      gagaaacaa caacgttgc gaaatcgtc tcggttcat 
      acgaattcg gatgatgaa aatatcgat ttgaaaaat 
      ttcgttgac gatatgtta gagttggat aaaatgagt 
      gaagagcac aggaaaagt gtgatcgag acacgaatc 
      ttggcttcc agttcgatt accaacctt ataaacaaa 
      caaatggca caagaagcc gatgtccag aaggcctac 
      aaaattttt gccgacttg tccgaaaag aaaaccaca 
      gaagagaag gaaaataaa gatctagtc actgatttt 
      gttctcatt gaacatgat tatgcaaaa attggcgaa 
      ttaccaagt caggtggaa gaaattaac gaactaact 
      cacgaagaa tcggtcaaa attagcgaa ccatcaact 
      gaagaagat caagaatcg gccaaaatt aacgaatta 
      tcaacggag gaagaagat gaagagtcg gtgcaaaac 
      agtcttagc cctgtccct attccggac ctggaagtg 
      ctccctgtt aacacagac attgagatc aaatcactg 
      catcgaagt ctcacacgg acgaaccgc cgaaagttg 
      tttttaaat aattacgga cttcataac tggaattcc 
      ggctcacat tcggataat cgaaaactg aaccattca 
      atttcaata acgaaagaa gacggtgct gttttaaga 
      ggtttttac caagatttt aatctggtc attgtccag 
      gagaagtcg gtttcgttt tggacgcag agtgcgttg 
      gggaatgtc ctgggggca caaaatatg tggatcccc 
      aaaggacaa atccagagg attcccctg aatagcacc 
      tatgtacaa aaagatgcc aatgaaatg gtcatttcg 
      actgagacc agtgtggca tacgttgaa ttatggacc 
      aaagaacac aagtcagat gtgagggaa gtaccggtt 
      gctgatgtt ttcgcgacg gtttatttc tggcgcttg 
      cggcagagc gggttggag aagaaggcg ttgcaattg 
      gagaatatt aaaggatgc gctgaggat gtgcgatat 
      tgcgtcctg cggtgttac cctcgcatt atcgtttgt 
      tggcaattg gtccaaggc aacgaaaaa cgtacaaaa 
      atccattct ttccattta gcggccgat tttcagacc 
      gttgccaac attagcgaa atacaaccg gttgaacat 
      tacgtatcg tcgttacac aacattgat gattgtgac 
      agtttaatc atgggttgt ggcgaaagt aaaatcact 
      ttgtggaat atcgaatat ggttacatc gtttccagt 
      attgaattg tcgaatatc aaatcgcct ttggccaca 
      cattgggtc aagtgtgat aggggtttt ctgttcatg 
      atccaagag tgcgctgat tatgagttg cgtttgatt 
      gcaataaat gggatgaat cattcgtgg aaaaagctc 
      cagaatttc gatccacca actggattt gaaggattg 
      aaaggagtt tgcgttgaa aatggcata gtgatagcc 
      tactattcg caaggtatt ttgtgctgg aatgcacaa 
      agtggcgaa ccaatagtc gaggaagta ccaaatgaa 
      tgtgaatat attccttct gggaaacat gtgatattg 
      ctaattgac gaaaatgtc atcgtacgg aattcgtta 
      agccatcta ataacagat ttgtaa
    mRNA sequence
    • >TC006801 RA
      ttcgtattt tttctatgt gtaaaaagg ccgttttgg 
      cgagaaacg tgtttgttt ggcggcgct gctgtcgca 
      gaaaaatca ccaaagcgt caaacaagc ccccttggt 
      attttttga ttgtttgac gtttttggg ggggttgta 
      gcgtccccc caaagtcag gtgtacaaa tgtgtgcta 
      aacaattaa attaagtga tcgttacca cgttaaact 
      ttcgtaatg gcaagtgtt atttccctc tcgagtgca 
      tcgttgaac gcttccccc aaaaacacc gattctcct 
      gtgactgtt ctaacccta agttcggaa atacaccga 
      gttcccgtg agttgggac ggccacaaa ggtgccaaa 
      aggacgtcc aaggtcttg gactctgat aacaccccc 
      aatcattac gaaaaagtc aaagaatgg atagatacg 
      accaagtac gatgatctg gggccccct aatgagaat 
      aaaagcgtt tgtatccgc tatgatttc gataaaagc 
      gagaaaatc acaataaat gtgccgaaa tcatgtttt 
      acgcgagtc actcccttg ttttcttat atggaacag 
      gccaaaaaa gcggctctg agtgacgag aaaaaaagc 
      caggaatgt acagcaaaa gtcaatgga aatttaaac 
      tttgaaact cccctaagc ccagaaagc cagaaaaag 
      gaactttgc agttactta cagctaatg aaacctggc 
      gaaaagaaa cagaagaaa ctgttattc ggaaacaga 
      cgatcgaag cgagtcaag aatttagtc caaaaccac 
      caaaaaacg tgttcagaa aacgacata aacaccttc 
      cccaccctc aaatcattc gaagatttg aacattttt 
      gagtcgaag aagaaaaaa gaaacgccc aataaatat 
      ttaccattg cccccaaag tcaaaattt agcgaattt 
      gttgatttt gaggactcg gttcgtaag attgtcgaa 
      agtgtcaag ttgaggagt aaagtgaca aagcgccga 
      aaatttaat tgggggctc aaatcgcat cagtttaat 
      ttcaaacga attaattca ctacgtagc aaaggtcgg 
      ctcaggttc atttctttg gatcggctc attttgcgt 
      aaaagtcgg aaacggcgg aaaaaattt ctcaagaaa 
      gattcacca caaaaaaat gcatgtccc cactgtgat 
      tcggtggag aaacaacaa cgttgcgaa atcgtctcg 
      gttcatacg aattcggat gatgaaaat atcgatttg 
      aaaaatttc gttgacgat atgttagag ttggataaa 
      atgagtgaa gagcacagg aaaagtgtg atcgagaca 
      cgaatcttg gcttccagt tcgattacc aaccttata 
      aacaaacaa atggcacaa gaagccgat gtccagaag 
      gcctacaaa atttttgcc gacttgtcc gaaaagaaa 
      accacagaa gagaaggaa aataaagat ctagtcact 
      gattttgtt ctcattgaa catgattat gcaaaaatt 
      ggcgaatta ccaagtcag gtggaagaa attaacgaa 
      ctaactcac gaagaatcg gtcaaaatt agcgaacca 
      tcaactgaa gaagatcaa gaatcggcc aaaattaac 
      gaattatca acggaggaa gaagatgaa gagtcggtg 
      caaaacagt cttagccct gtccctatt ccggacctg 
      gaagtgctc cctgttaac acagacatt gagatcaaa 
      tcactgcat cgaagtctc acacggacg aaccgccga 
      aagttgttt ttaaataat tacggactt cataactgg 
      aattccggc tcacattcg gataatcga aaactgaac 
      cattcaatt tcaataacg aaagaagac ggtgctgtt 
      ttaagaggt ttttaccaa gattttaat ctggtcatt 
      gtccaggag aagtcggtt tcgttttgg acgcagagt 
      gcgttgggg aatgtcctg ggggcacaa aatatgtgg 
      atccccaaa ggacaaatc cagaggatt cccctgaat 
      agcacctat gtacaaaaa gatgccaat gaaatggtc 
      atttcgact gagaccagt gtggcatac gttgaatta 
      tggaccaaa gaacacaag tcagatgtg agggaagta 
      ccggttgct gatgttttc gcgacggtt tatttctgg 
      cgcttgcgg cagagcggg ttggagaag aaggcgttg 
      caattggag aatattaaa ggatgcgct gaggatgtg 
      cgatattgc gtcctgcgg tgttaccct cgcattatc 
      gtttgttgg caattggtc caaggcaac gaaaaacgt 
      acaaaaatc cattctttc catttagcg gccgatttt 
      cagaccgtt gccaacatt agcgaaata caaccggtt 
      gaacattac gtatcgtcg ttacacaac attgatgat 
      tgtgacagt ttaatcatg ggttgtggc gaaagtaaa 
      atcactttg tggaatatc gaatatggt tacatcgtt 
      tccagtatt gaattgtcg aatatcaaa tcgcctttg 
      gccacacat tgggtcaag tgtgatagg ggttttctg 
      ttcatgatc caagagtgc gctgattat gagttgcgt 
      ttgattgca ataaatggg atgaatcat tcgtggaaa 
      aagctccag aatttcgat ccaccaact ggatttgaa 
      ggattgaaa ggagtttgc gttgaaaat ggcatagtg 
      atagcctac tattcgcaa ggtattttg tgctggaat 
      gcacaaagt ggcgaacca atagtcgag gaagtacca 
      aatgaatgt gaatatatt ccttctggg aaacatgtg 
      atattgcta attgacgaa aatgtcatc gtacggaat 
      tcgttaagc catctaata acagatttg taagaattt 
      gtttcctat ttgttgttt tgttgactg atattaggt 
      actctagag tgttattat tattattta tgtgaatat 
      aatttagtt gcaattgtg cagtttcct gtaaaaaga 
      aatttgcta ttttgacga ataaatatt ttctatgaa 
      a
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