Status
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Gene information
General information
TC006801 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14382314..14385515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006801
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04215

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head bristle number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gena triplet potentially position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    vertex triplet vertex triplet ventral potentially position ventralized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    bell row anteroposterior-axis elongated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006801 RA
      MASVISLSS ASLNASPKN TDSPVTVLT LSSEIHRVP 
VSWDGHKGA KRTSKVLDS DNTPNHYEK VKEWIDTTK 
YDDLGPPNE NKSVCIRYD FDKSEKITI NVPKSCFTR 
VTPLFSYME QAKKAALSD EKKSQECTA KVNGNLNFE 
TPLSPESQK KELCSYLQL MKPGEKKQK KLLFGNRRS 
KRVKNLVQN HQKTCSEND INTFPTLKS FEDLNIFES 
KKKKETPNK YLPLPPKSK FSEFVDFED SVRKIVESV 
KLRSKVTKR RKFNWGLKS HQFNFKRIN SLRSKGRLR 
FISLDRLIL RKSRKRRKK FLKKDSPQK NACPHCDSV 
EKQQRCEIV SVHTNSDDE NIDLKNFVD DMLELDKMS 
EEHRKSVIE TRILASSSI TNLINKQMA QEADVQKAY 
KIFADLSEK KTTEEKENK DLVTDFVLI EHDYAKIGE 
LPSQVEEIN ELTHEESVK ISEPSTEED QESAKINEL 
STEEEDEES VQNSLSPVP IPDLEVLPV NTDIEIKSL 
HRSLTRTNR RKLFLNNYG LHNWNSGSH SDNRKLNHS 
ISITKEDGA VLRGFYQDF NLVIVQEKS VSFWTQSAL 
GNVLGAQNM WIPKGQIQR IPLNSTYVQ KDANEMVIS 
TETSVAYVE LWTKEHKSD VREVPVADV FATVYFWRL 
RQSGLEKKA LQLENIKGC AEDVRYCVL RCYPRIIVC 
WQLVQGNEK RTKIHSFHL AADFQTVAN ISEIQPVEH 
YVSSLHNID DCDSLIMGC GESKITLWN IEYGYIVSS 
IELSNIKSP LATHWVKCD RGFLFMIQE CADYELRLI 
AINGMNHSW KKLQNFDPP TGFEGLKGV CVENGIVIA 
YYSQGILCW NAQSGEPIV EEVPNECEY IPSGKHVIL 
LIDENVIVR NSLSHLITD L
    CDS sequence
    • >TC006801 RA
      atggcaagt gttatttcc ctctcgagt gcatcgttg 
aacgcttcc cccaaaaac accgattct cctgtgact 
gttctaacc ctaagttcg gaaatacac cgagttccc 
gtgagttgg gacggccac aaaggtgcc aaaaggacg 
tccaaggtc ttggactct gataacacc cccaatcat 
tacgaaaaa gtcaaagaa tggatagat acgaccaag 
tacgatgat ctggggccc cctaatgag aataaaagc 
gtttgtatc cgctatgat ttcgataaa agcgagaaa 
atcacaata aatgtgccg aaatcatgt tttacgcga 
gtcactccc ttgttttct tatatggaa caggccaaa 
aaagcggct ctgagtgac gagaaaaaa agccaggaa 
tgtacagca aaagtcaat ggaaattta aactttgaa 
actccccta agcccagaa agccagaaa aaggaactt 
tgcagttac ttacagcta atgaaacct ggcgaaaag 
aaacagaag aaactgtta ttcggaaac agacgatcg 
aagcgagtc aagaattta gtccaaaac caccaaaaa 
acgtgttca gaaaacgac ataaacacc ttccccacc 
ctcaaatca ttcgaagat ttgaacatt tttgagtcg 
aagaagaaa aaagaaacg cccaataaa tatttacca 
ttgccccca aagtcaaaa tttagcgaa tttgttgat 
tttgaggac tcggttcgt aagattgtc gaaagtgtc 
aagttgagg agtaaagtg acaaagcgc cgaaaattt 
aattggggg ctcaaatcg catcagttt aatttcaaa 
cgaattaat tcactacgt agcaaaggt cggctcagg 
ttcatttct ttggatcgg ctcattttg cgtaaaagt 
cggaaacgg cggaaaaaa tttctcaag aaagattca 
ccacaaaaa aatgcatgt ccccactgt gattcggtg 
gagaaacaa caacgttgc gaaatcgtc tcggttcat 
acgaattcg gatgatgaa aatatcgat ttgaaaaat 
ttcgttgac gatatgtta gagttggat aaaatgagt 
gaagagcac aggaaaagt gtgatcgag acacgaatc 
ttggcttcc agttcgatt accaacctt ataaacaaa 
caaatggca caagaagcc gatgtccag aaggcctac 
aaaattttt gccgacttg tccgaaaag aaaaccaca 
gaagagaag gaaaataaa gatctagtc actgatttt 
gttctcatt gaacatgat tatgcaaaa attggcgaa 
ttaccaagt caggtggaa gaaattaac gaactaact 
cacgaagaa tcggtcaaa attagcgaa ccatcaact 
gaagaagat caagaatcg gccaaaatt aacgaatta 
tcaacggag gaagaagat gaagagtcg gtgcaaaac 
agtcttagc cctgtccct attccggac ctggaagtg 
ctccctgtt aacacagac attgagatc aaatcactg 
catcgaagt ctcacacgg acgaaccgc cgaaagttg 
tttttaaat aattacgga cttcataac tggaattcc 
ggctcacat tcggataat cgaaaactg aaccattca 
atttcaata acgaaagaa gacggtgct gttttaaga 
ggtttttac caagatttt aatctggtc attgtccag 
gagaagtcg gtttcgttt tggacgcag agtgcgttg 
gggaatgtc ctgggggca caaaatatg tggatcccc 
aaaggacaa atccagagg attcccctg aatagcacc 
tatgtacaa aaagatgcc aatgaaatg gtcatttcg 
actgagacc agtgtggca tacgttgaa ttatggacc 
aaagaacac aagtcagat gtgagggaa gtaccggtt 
gctgatgtt ttcgcgacg gtttatttc tggcgcttg 
cggcagagc gggttggag aagaaggcg ttgcaattg 
gagaatatt aaaggatgc gctgaggat gtgcgatat 
tgcgtcctg cggtgttac cctcgcatt atcgtttgt 
tggcaattg gtccaaggc aacgaaaaa cgtacaaaa 
atccattct ttccattta gcggccgat tttcagacc 
gttgccaac attagcgaa atacaaccg gttgaacat 
tacgtatcg tcgttacac aacattgat gattgtgac 
agtttaatc atgggttgt ggcgaaagt aaaatcact 
ttgtggaat atcgaatat ggttacatc gtttccagt 
attgaattg tcgaatatc aaatcgcct ttggccaca 
cattgggtc aagtgtgat aggggtttt ctgttcatg 
atccaagag tgcgctgat tatgagttg cgtttgatt 
gcaataaat gggatgaat cattcgtgg aaaaagctc 
cagaatttc gatccacca actggattt gaaggattg 
aaaggagtt tgcgttgaa aatggcata gtgatagcc 
tactattcg caaggtatt ttgtgctgg aatgcacaa 
agtggcgaa ccaatagtc gaggaagta ccaaatgaa 
tgtgaatat attccttct gggaaacat gtgatattg 
ctaattgac gaaaatgtc atcgtacgg aattcgtta 
agccatcta ataacagat ttgtaa
    mRNA sequence
    • >TC006801 RA
      ttcgtattt tttctatgt gtaaaaagg ccgttttgg 
cgagaaacg tgtttgttt ggcggcgct gctgtcgca 
gaaaaatca ccaaagcgt caaacaagc ccccttggt 
attttttga ttgtttgac gtttttggg ggggttgta 
gcgtccccc caaagtcag gtgtacaaa tgtgtgcta 
aacaattaa attaagtga tcgttacca cgttaaact 
ttcgtaatg gcaagtgtt atttccctc tcgagtgca 
tcgttgaac gcttccccc aaaaacacc gattctcct 
gtgactgtt ctaacccta agttcggaa atacaccga 
gttcccgtg agttgggac ggccacaaa ggtgccaaa 
aggacgtcc aaggtcttg gactctgat aacaccccc 
aatcattac gaaaaagtc aaagaatgg atagatacg 
accaagtac gatgatctg gggccccct aatgagaat 
aaaagcgtt tgtatccgc tatgatttc gataaaagc 
gagaaaatc acaataaat gtgccgaaa tcatgtttt 
acgcgagtc actcccttg ttttcttat atggaacag 
gccaaaaaa gcggctctg agtgacgag aaaaaaagc 
caggaatgt acagcaaaa gtcaatgga aatttaaac 
tttgaaact cccctaagc ccagaaagc cagaaaaag 
gaactttgc agttactta cagctaatg aaacctggc 
gaaaagaaa cagaagaaa ctgttattc ggaaacaga 
cgatcgaag cgagtcaag aatttagtc caaaaccac 
caaaaaacg tgttcagaa aacgacata aacaccttc 
cccaccctc aaatcattc gaagatttg aacattttt 
gagtcgaag aagaaaaaa gaaacgccc aataaatat 
ttaccattg cccccaaag tcaaaattt agcgaattt 
gttgatttt gaggactcg gttcgtaag attgtcgaa 
agtgtcaag ttgaggagt aaagtgaca aagcgccga 
aaatttaat tgggggctc aaatcgcat cagtttaat 
ttcaaacga attaattca ctacgtagc aaaggtcgg 
ctcaggttc atttctttg gatcggctc attttgcgt 
aaaagtcgg aaacggcgg aaaaaattt ctcaagaaa 
gattcacca caaaaaaat gcatgtccc cactgtgat 
tcggtggag aaacaacaa cgttgcgaa atcgtctcg 
gttcatacg aattcggat gatgaaaat atcgatttg 
aaaaatttc gttgacgat atgttagag ttggataaa 
atgagtgaa gagcacagg aaaagtgtg atcgagaca 
cgaatcttg gcttccagt tcgattacc aaccttata 
aacaaacaa atggcacaa gaagccgat gtccagaag 
gcctacaaa atttttgcc gacttgtcc gaaaagaaa 
accacagaa gagaaggaa aataaagat ctagtcact 
gattttgtt ctcattgaa catgattat gcaaaaatt 
ggcgaatta ccaagtcag gtggaagaa attaacgaa 
ctaactcac gaagaatcg gtcaaaatt agcgaacca 
tcaactgaa gaagatcaa gaatcggcc aaaattaac 
gaattatca acggaggaa gaagatgaa gagtcggtg 
caaaacagt cttagccct gtccctatt ccggacctg 
gaagtgctc cctgttaac acagacatt gagatcaaa 
tcactgcat cgaagtctc acacggacg aaccgccga 
aagttgttt ttaaataat tacggactt cataactgg 
aattccggc tcacattcg gataatcga aaactgaac 
cattcaatt tcaataacg aaagaagac ggtgctgtt 
ttaagaggt ttttaccaa gattttaat ctggtcatt 
gtccaggag aagtcggtt tcgttttgg acgcagagt 
gcgttgggg aatgtcctg ggggcacaa aatatgtgg 
atccccaaa ggacaaatc cagaggatt cccctgaat 
agcacctat gtacaaaaa gatgccaat gaaatggtc 
atttcgact gagaccagt gtggcatac gttgaatta 
tggaccaaa gaacacaag tcagatgtg agggaagta 
ccggttgct gatgttttc gcgacggtt tatttctgg 
cgcttgcgg cagagcggg ttggagaag aaggcgttg 
caattggag aatattaaa ggatgcgct gaggatgtg 
cgatattgc gtcctgcgg tgttaccct cgcattatc 
gtttgttgg caattggtc caaggcaac gaaaaacgt 
acaaaaatc cattctttc catttagcg gccgatttt 
cagaccgtt gccaacatt agcgaaata caaccggtt 
gaacattac gtatcgtcg ttacacaac attgatgat 
tgtgacagt ttaatcatg ggttgtggc gaaagtaaa 
atcactttg tggaatatc gaatatggt tacatcgtt 
tccagtatt gaattgtcg aatatcaaa tcgcctttg 
gccacacat tgggtcaag tgtgatagg ggttttctg 
ttcatgatc caagagtgc gctgattat gagttgcgt 
ttgattgca ataaatggg atgaatcat tcgtggaaa 
aagctccag aatttcgat ccaccaact ggatttgaa 
ggattgaaa ggagtttgc gttgaaaat ggcatagtg 
atagcctac tattcgcaa ggtattttg tgctggaat 
gcacaaagt ggcgaacca atagtcgag gaagtacca 
aatgaatgt gaatatatt ccttctggg aaacatgtg 
atattgcta attgacgaa aatgtcatc gtacggaat 
tcgttaagc catctaata acagatttg taagaattt 
gtttcctat ttgttgttt tgttgactg atattaggt 
actctagag tgttattat tattattta tgtgaatat 
aatttagtt gcaattgtg cagtttcct gtaaaaaga 
aatttgcta ttttgacga ataaatatt ttctatgaa 
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