Status
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Gene information
General information
TC006783 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14202540..14204821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006783
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04212

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      remarks: animals lost,
      Adult morphology
      remarks: animals lost,
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006783 RA
        MLSLEKLAN ANIKNRAEY TQLSTELNR LEKEKKNEL 
        RMFNNEIQR FKSKYSKSN FSTDSPEAS NVKMYETHN 
        PGFCYKRNG EHRVLQVSE NTPPVDFER LHFLLFTNG 
        EVDIEKILC LHLLSSHET DHLTPPRRP RTSGGFMKK 
        DSPVEQLIE RSKENPREA VLRAILPTY ILDVNKGHT 
        KRTLLDERL SARLIVNEK ILELIMTVI TALENSNPE 
        KVQHLISLA EDPSSLKRI FRKQHIQDF VMTLKNHSL 
        VNSCSSFIL TQPNKVTPS HIAIRTPTG ALEDLVRYI 
        RANVLQFPK RSSFNLIEP ESRELLTIE RSKMSTKVS 
        NLSTKPITI TMPKPEFRK ESKDEASRF RRKSTNFIK 
        KNIESIPNY KRGSEDENF TNKPKKIRR ASTDSSRRT 
        VPNSRKSST TSDRSMLDS PLKKHEHKN DEIKVHLKQ 
        TTCPACMEK WKIPLPSPK VPRYKRPSS GSTSSEVPQ 
        PIIKTDSMR RISNMIEKE QFVLSSYDR RYSNVSSGS 
        NQSRRKSSV QSGSLDLVA LNILDLEKI RHEIREKET 
        QKKLEKFLG A
      CDS sequence
      • >TC006783 RA
        atgctttcg cttgaaaaa ctcgccaat gccaatatt 
        aaaaatcgc gctgagtat acacaactt tccaccgaa 
        ttaaaccga ctcgaaaaa gagaaaaag aatgagttg 
        cgaatgttt aacaatgag atacagcgc ttcaaatcg 
        aaatattcc aagtccaac ttcagcacg gattcaccc 
        gaggcctct aatgttaaa atgtacgaa acgcacaac 
        ccgggattt tgctacaag aggaatggg gagcacagg 
        gtgttgcaa gtcagcgaa aacacgccg ccagtcgat 
        ttcgaaaga ctacatttt ttactgttc acaaatggc 
        gaagtagat attgaaaaa atcctttgt cttcattta 
        ctctcctcg catgaaacg gaccacttg acaccacct 
        aggagacct cgaacttcc ggtggtttc atgaaaaaa 
        gactcgcca gtagagcaa ctaatcgaa cggtcgaag 
        gaaaaccca cgagaggct gtcttaaga gcaattcta 
        ccgacctat atccttgat gtaaataaa ggtcacact 
        aaacgaacc cttttggac gaaagactg tcagctcgt 
        ctgatagtc aacgaaaaa attctcgaa ctgattatg 
        accgtgatc acggctttg gaaaactca aaccctgaa 
        aaagtccaa cacttgata agtctagct gaagacccg 
        agcagcctg aaacgaatt ttcaggaag caacacatc 
        caagacttc gtaatgacg ctgaaaaat cactcgttg 
        gtcaacagt tgcagttcg tttattttg actcaacct 
        aacaaagtc actcctagt catatcgca attcggaca 
        ccgactggt gcccttgaa gacctggtc cgttatatc 
        cgtgcaaat gtcttgcag tttcccaaa cggagcagt 
        tttaatctc attgaacca gaatctaga gaactcctc 
        acaattgaa cgaagcaaa atgtcgacc aaagttagt 
        aacttaagc acgaaaccg atcacaata acgatgcca 
        aaaccagag tttcggaaa gaatcaaag gacgaagcg 
        tcacgtttt cgtcggaaa agcaccaac tttatcaag 
        aaaaatatc gagtcaatc ccgaattat aagcgaggt 
        tcggaagat gaaaatttc acaaataag ccgaaaaaa 
        atcagacgt gcgagcacc gactccagt cgaagaacg 
        gttccaaat agccgcaaa agtagcacc actagtgat 
        agaagcatg ctggactct cctctcaaa aaacatgag 
        cataagaat gacgaaatt aaagtacat ttgaagcaa 
        acaacgtgt cctgcgtgt atggagaag tggaagatc 
        ccgcttcct tcgcccaaa gtgccgcgg tacaagcga 
        ccaagttcg ggatcaact tccagcgaa gtgcctcaa 
        ccaatcatt aaaacggac agtatgcga agaattagt 
        aacatgatt gagaaggaa cagtttgtt ttgagttcg 
        tacgaccgc agatattcc aatgtgagc agtgggtcg 
        aaccaaagc aggcgaaaa tcgagtgtt cagagcggt 
        tctttggac ttggtcgct ttgaatatc ttggacttg 
        gagaaaatt cggcatgaa attcgggag aaggagacg 
        cagaagaag cttgagaag tttctaggt gcttga
      mRNA sequence
      • >TC006783 RA
        tggagcata aacaagagg atgaacagt agatggtaa 
        tctttgata gattttgta ataactttt caagaaaaa 
        actataatc gctgcttgt gggggaaaa acaaaaccg 
        ctcataatt gctgggtgt cgactggct ccattaaaa 
        atttaaata caattattt tcggaaaaa tgtcgaatt 
        tttccacta caatacacg cacctgcat tgtttcaat 
        catgtctaa attgtcaat ttgcgaaat ttgtcactg 
        gcgacatgc tttcgcttg aaaaactcg ccaatgcca 
        atattaaaa atcgcgctg agtatacac aactttcca 
        ccgaattaa accgactcg aaaaagaga aaaagaatg 
        agttgcgaa tgtttaaca atgagatac agcgcttca 
        aatcgaaat attccaagt ccaacttca gcacggatt 
        cacccgagg cctctaatg ttaaaatgt acgaaacgc 
        acaacccgg gattttgct acaagagga atggggagc 
        acagggtgt tgcaagtca gcgaaaaca cgccgccag 
        tcgatttcg aaagactac attttttac tgttcacaa 
        atggcgaag tagatattg aaaaaatcc tttgtcttc 
        atttactct cctcgcatg aaacggacc acttgacac 
        cacctagga gacctcgaa cttccggtg gtttcatga 
        aaaaagact cgccagtag agcaactaa tcgaacggt 
        cgaaggaaa acccacgag aggctgtct taagagcaa 
        ttctaccga cctatatcc ttgatgtaa ataaaggtc 
        acactaaac gaacccttt tggacgaaa gactgtcag 
        ctcgtctga tagtcaacg aaaaaattc tcgaactga 
        ttatgaccg tgatcacgg ctttggaaa actcaaacc 
        ctgaaaaag tccaacact tgataagtc tagctgaag 
        acccgagca gcctgaaac gaattttca ggaagcaac 
        acatccaag acttcgtaa tgacgctga aaaatcact 
        cgttggtca acagttgca gttcgttta ttttgactc 
        aacctaaca aagtcactc ctagtcata tcgcaattc 
        ggacaccga ctggtgccc ttgaagacc tggtccgtt 
        atatccgtg caaatgtct tgcagtttc ccaaacgga 
        gcagtttta atctcattg aaccagaat ctagagaac 
        tcctcacaa ttgaacgaa gcaaaatgt cgaccaaag 
        ttagtaact taagcacga aaccgatca caataacga 
        tgccaaaac cagagtttc ggaaagaat caaaggacg 
        aagcgtcac gttttcgtc ggaaaagca ccaacttta 
        tcaagaaaa atatcgagt caatcccga attataagc 
        gaggttcgg aagatgaaa atttcacaa ataagccga 
        aaaaaatca gacgtgcga gcaccgact ccagtcgaa 
        gaacggttc caaatagcc gcaaaagta gcaccacta 
        gtgatagaa gcatgctgg actctcctc tcaaaaaac 
        atgagcata agaatgacg aaattaaag tacatttga 
        agcaaacaa cgtgtcctg cgtgtatgg agaagtgga 
        agatcccgc ttccttcgc ccaaagtgc cgcggtaca 
        agcgaccaa gttcgggat caacttcca gcgaagtgc 
        ctcaaccaa tcattaaaa cggacagta tgcgaagaa 
        ttagtaaca tgattgaga aggaacagt ttgttttga 
        gttcgtacg accgcagat attccaatg tgagcagtg 
        ggtcgaacc aaagcaggc gaaaatcga gtgttcaga 
        gcggttctt tggacttgg tcgctttga atatcttgg 
        acttggaga aaattcggc atgaaattc gggagaagg 
        agacgcaga agaagcttg agaagtttc taggtgctt 
        gaagctcga ataaagcac tttcactgt g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY