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Gene information
General information
TC006773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14016599..14019743
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006773
      iBeetle screen iB_04210

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      abdomen segment potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006773 RA
        MAPTANFLS QPTNSNHIG AQIETMNCS 
        MLNTNWMQL SHQAEPFQP KLLSPTWST 
        FITPCVNYS PSQDTLSLS DLTEQFADL 
        ALDKRTLKR PPPSYMCHL CFQKGHYIK 
        DCPKARPKG EGKTPYQGR KRCFGEFKC 
        PKCKRKWMS GNSWANIGQ DCIKCQINV 
        YPHKQRPLE KPDGLDVSD QSKVHPQHL 
        CQKCRELGF YCRRVLQ
      • >TC006773 RB
        MVDLLSFFT DSDVLETRL RKMAPTANF 
        LSQPTNSNH IGAQIETMN CSMLNTNWM 
        QLSHQAEPF QPKLLSPTW STFITPCVN 
        YSPSQDTLS LSDLTEQFA DLALDKRTL 
        KRPPPSYMC HLCFQKGHY IKDCPKARP 
        KGEGKTPYQ GRKRCFGEF KCPKCKRKW 
        MSGNSWANI GQDCIKCQI NVYPHKQRP 
        LEKPDGLDV SDQSKVHPQ HLCQKCREL 
        GFYCRRVLQ
      CDS sequence
      • >TC006773 RA
        atggcccca actgctaac tttcttagt caaccgacg 
        aatagtaac cacattggg gcccaaatc gaaaccatg 
        aactgttca atgctcaac accaattgg atgcaactc 
        tcgcatcag gctgaaccc tttcagccc aagctcttg 
        tctccgact tggagcact tttataaca ccatgtgtc 
        aactacagt ccatcgcag gatacgctc tccctgagc 
        gatcttacg gaacaattt gctgatctg gctttggat 
        aaacgcact ttgaaaagg ccaccaccg tcctacatg 
        tgccatttg tgcttccag aagggacac tacatcaaa 
        gattgcccc aaggcgcgt cctaaaggc gaaggcaaa 
        accccttat caaggccgc aaacgttgt tttggcgaa 
        tttaaatgt cccaaatgt aagaggaag tggatgagc 
        gggaactcg tgggcgaat attggccaa gattgcatc 
        aaatgccaa attaatgtg tatccgcat aaacagcga 
        cctttggag aaaccggac gggctggac gtttcggac 
        caatcgaag gtgcatcct cagcacctg tgccagaag 
        tgccgcgag ttggggttt tactgcagg agggtgctg 
        cagtaa
      • >TC006773 RB
        atggtcgat ttgttgtcg tttttcact gactctgat 
        gtgcttgaa accaggcta agaaaaatg gccccaact 
        gctaacttt cttagtcaa ccgacgaat agtaaccac 
        attggggcc caaatcgaa accatgaac tgttcaatg 
        ctcaacacc aattggatg caactctcg catcaggct 
        gaacccttt cagcccaag ctcttgtct ccgacttgg 
        agcactttt ataacacca tgtgtcaac tacagtcca 
        tcgcaggat acgctctcc ctgagcgat cttacggaa 
        caatttgct gatctggct ttggataaa cgcactttg 
        aaaaggcca ccaccgtcc tacatgtgc catttgtgc 
        ttccagaag ggacactac atcaaagat tgccccaag 
        gcgcgtcct aaaggcgaa ggcaaaacc ccttatcaa 
        ggccgcaaa cgttgtttt ggcgaattt aaatgtccc 
        aaatgtaag aggaagtgg atgagcggg aactcgtgg 
        gcgaatatt ggccaagat tgcatcaaa tgccaaatt 
        aatgtgtat ccgcataaa cagcgacct ttggagaaa 
        ccggacggg ctggacgtt tcggaccaa tcgaaggtg 
        catcctcag cacctgtgc cagaagtgc cgcgagttg 
        gggttttac tgcaggagg gtgctgcag taa
      mRNA sequence
      • >TC006773 RA
        tcgttatga gcacataag gagcattta ccaccgaac 
        ccgaatctg gctaagaaa aatggcccc aactgctaa 
        ctttcttag tcaaccgac gaatagtaa ccacattgg 
        ggcccaaat cgaaaccat gaactgttc aatgctcaa 
        caccaattg gatgcaact ctcgcatca ggctgaacc 
        ctttcagcc caagctctt gtctccgac ttggagcac 
        ttttataac accatgtgt caactacag tccatcgca 
        ggatacgct ctccctgag cgatcttac ggaacaatt 
        tgctgatct ggctttgga taaacgcac tttgaaaag 
        gccaccacc gtcctacat gtgccattt gtgcttcca 
        gaagggaca ctacatcaa agattgccc caaggcgcg 
        tcctaaagg cgaaggcaa aacccctta tcaaggccg 
        caaacgttg ttttggcga atttaaatg tcccaaatg 
        taagaggaa gtggatgag cgggaactc gtgggcgaa 
        tattggcca agattgcat caaatgcca aattaatgt 
        gtatccgca taaacagcg acctttgga gaaaccgga 
        cgggctgga cgtttcgga ccaatcgaa ggtgcatcc 
        tcagcacct gtgccagaa gtgccgcga gttggggtt 
        ttactgcag gagggtgct gcagtaaag tgatttttt 
        acaccattt atatttttt tattttgag aggtttttg 
        ttcttttt
      • >TC006773 RB
        tcgttatga gcacataag gagcattta ccaccgaac 
        ccgaatctg ttttgcact aacaatggt cgatttgtt 
        gtcgttttt cactgactc tgatgtgct tgaaaccag 
        gctaagaaa aatggcccc aactgctaa ctttcttag 
        tcaaccgac gaatagtaa ccacattgg ggcccaaat 
        cgaaaccat gaactgttc aatgctcaa caccaattg 
        gatgcaact ctcgcatca ggctgaacc ctttcagcc 
        caagctctt gtctccgac ttggagcac ttttataac 
        accatgtgt caactacag tccatcgca ggatacgct 
        ctccctgag cgatcttac ggaacaatt tgctgatct 
        ggctttgga taaacgcac tttgaaaag gccaccacc 
        gtcctacat gtgccattt gtgcttcca gaagggaca 
        ctacatcaa agattgccc caaggcgcg tcctaaagg 
        cgaaggcaa aacccctta tcaaggccg caaacgttg 
        ttttggcga atttaaatg tcccaaatg taagaggaa 
        gtggatgag cgggaactc gtgggcgaa tattggcca 
        agattgcat caaatgcca aattaatgt gtatccgca 
        taaacagcg acctttgga gaaaccgga cgggctgga 
        cgtttcgga ccaatcgaa ggtgcatcc tcagcacct 
        gtgccagaa gtgccgcga gttggggtt ttactgcag 
        gagggtgct gcagtaaag tgatttttt acaccattt 
        atatttttt tattttgag aggtttttg ttcttttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY