General information | |||
TC006735 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13539378..13565746 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC006735 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04205 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC006735 RA
MCVCCGGGF LRVVLVLWL CGPSMHFRI SKREIVDDS VQRYLTRFG YMDATADGA FALRTEESV RDAIKDMQE FAGIPVTGR LDERTLKLL NTPRCGMPD KNKQTSGRR KRFTLHGQK WPYTNLTWS LRSRDLMGM DPYQVRLVI SKALDVWAR HSKLTFTEV DSPKADILI FFVRGEHGD NFPFDGKGV ILAHAFFPN GGHSIDVHF DADEAWTTV PNSDEGTNL FNVAAHEFG HSLGLSHSS EEGALMYPW YKEMENGFD YELPDDDKL GIQALYGAR TTRLWDRIK PYRPPTTTT TTTTTTTRR PYFRPSQRP FNPNGRHPH NPYNPYHPH KNNPKKPMH PDKKYHNPK EHPGRYPVH RYTTARPTP PPRFPDKYP DWRHHNSDK EGPKRPPDT CDTSYDAVA VIRREVFFF KDEYFWRID ETGVLMPGY PAEIRRLWR DLPPNFTHV DAVYERPDN KIVFFIDDR FYVFTGNRL EPGYPRPLT DLGLPETLK KIDGAMIWG HNGKTYFYS GSMYWRFDE EERKVELDY PRDMSMWSG VGTDIDAVF QWKDGRTYF FKGTGFWKF NDIYMRVEH RQQKPSGPF WMGCSNNLE GNDVLQKLP YKHFAASGS SCRYLDVTA ALLVILLYY RF
- >TC006735 RA
atgtgtgtg tgctgtggt ggtggtttc ctgcgggtg gttctagtg ctgtggctg tgcggacct tcgatgcac tttaggatt agcaaaagg gaaattgtc gatgattcg gtgcaacgc tacttgacc cgctttggc tacatggac gccacggca gatggcgct ttcgccctt cgaacggag gaatctgtg cgggacgcc attaaagac atgcaagag tttgcgggt attcccgta acggggcgc ttagatgaa cgaacactt aaattactc aatacgcct cgttgcggg atgcctgat aaaaataaa caaacatca ggcagaagg aaaagattc actttacac ggccagaaa tggccgtac acgaatcta acatggagc ttgaggagc cgggatttg atgggaatg gacccgtac caagtccgg ctcgtcatt tccaaagct ttggacgtt tgggcaaga cattccaaa cttaccttc acggaagtt gacagccct aaggcggat atactcatc tttttcgtt agaggcgaa cacggagac aatttcccc tttgacggc aaaggagtg attttggcg cacgctttt ttcccaaac ggtggccac agcatcgac gtccacttc gacgcagat gaggcctgg acaactgtg cccaacagc gatgaaggg actaattta ttcaatgtg gcggcccac gaattcggc cactccttg ggcctatcg cactcttcc gaagagggc gctctgatg tacccatgg tacaaagaa atggaaaat gggttcgac tacgaactc cccgacgac gacaaattg ggcattcag gccctttat ggtgccaga accacgcga ttgtgggac aggatcaag ccctacagg ccccccact accacaact acaaccacc acgaccacg acccgacgg ccctatttc aggcccagt caacgtcct tttaatccc aacgggcga catccacac aatccttac aatccctac catccacat aaaaacaat cccaaaaaa cccatgcat cccgataag aagtaccac aatcctaag gagcatcca gggaggtac ccggtgcat aggtacacg acggcgcga ccgacgcct ccgccgagg ttcccagat aagtacccc gactggagg caccataac agtgataag gagggcccc aaaaggccc ccagatacg tgcgatacg agctacgat gccgtggcg gtgattcgg agggaggtt ttcttcttc aaggacgaa tacttttgg agaatcgac gaaaccggc gtgttaatg ccgggctac cctgcggag atccgtcgc ttgtggcgc gaccttccg cccaatttc acgcacgtg gacgccgtc tacgaacgg cccgacaac aaaatcgtt tttttcatc gacgatcgg ttttacgtt ttcaccgga aatcgactc gagccgggt tacccgcgc cccttaacc gacctgggg ctccccgaa actttgaaa aaaatcgac ggggcgatg atttggggc cacaacggc aagacctac ttctacagc ggctccatg tactggaga ttcgacgaa gaggagaga aaagtcgag ttggattat cctagagat atgtccatg tggagcgga gtggggacg gacattgac gcagtcttc cagtggaaa gacgggaga acgtatttt ttcaaaggg accggtttc tggaaattc aacgacatc tacatgcgg gtggagcac cggcagcag aagccgtct gggcccttc tggatgggc tgcagcaac aacctggag ggcaacgac gtcctgcag aagctgccc tacaagcac ttcgcagcg tctgggagt tcgtgtcgc tatctggat gtgacagcc gccttgctt gtaattctt ttgtattat cggttttaa
- >TC006735 RA
tagttcata aaaaacaac tctccattc tcgtgtagt cagtcctca gctggtgtt gctcggctc cgggtctct attttcgcc gtccagcct gaaataacg caccctctg aacacctcc gctcgctcc acagcatgg ccacgctgt gggaaatac gaatttcgg tttatcgga aaaacgtac gctgttgcg gtttctgta tttcctaac gcttgtgct tcctcggaa ccggagaaa tgtgtgttt gatataaac cggcgagtg gatgaaaac tggacattt tccgcgatg tgtgtgtgc tgtggtggt ggtttcctg cgggtggtt ctagtgctg tggctgtgc ggaccttcg atgcacttt aggattagc aaaagggaa attgtcgat gattcggtg caacgctac ttgacccgc tttggctac atggacgcc acggcagat ggcgctttc gcccttcga acggaggaa tctgtgcgg gacgccatt aaagacatg caagagttt gcgggtatt cccgtaacg gggcgctta gatgaacga acacttaaa ttactcaat acgcctcgt tgcgggatg cctgataaa aataaacaa acatcaggc agaaggaaa agattcact ttacacggc cagaaatgg ccgtacacg aatctaaca tggagcttg aggagccgg gatttgatg ggaatggac ccgtaccaa gtccggctc gtcatttcc aaagctttg gacgtttgg gcaagacat tccaaactt accttcacg gaagttgac agccctaag gcggatata ctcatcttt ttcgttaga ggcgaacac ggagacaat ttccccttt gacggcaaa ggagtgatt ttggcgcac gcttttttc ccaaacggt ggccacagc atcgacgtc cacttcgac gcagatgag gcctggaca actgtgccc aacagcgat gaagggact aatttattc aatgtggcg gcccacgaa ttcggccac tccttgggc ctatcgcac tcttccgaa gagggcgct ctgatgtac ccatggtac aaagaaatg gaaaatggg ttcgactac gaactcccc gacgacgac aaattgggc attcaggcc ctttatggt gccagaacc acgcgattg tgggacagg atcaagccc tacaggccc cccactacc acaactaca accaccacg accacgacc cgacggccc tatttcagg cccagtcaa cgtcctttt aatcccaac gggcgacat ccacacaat ccttacaat ccctaccat ccacataaa aacaatccc aaaaaaccc atgcatccc gataagaag taccacaat cctaaggag catccaggg aggtacccg gtgcatagg tacacgacg gcgcgaccg acgcctccg ccgaggttc ccagataag taccccgac tggaggcac cataacagt gataaggag ggccccaaa aggccccca gatacgtgc gatacgagc tacgatgcc gtggcggtg attcggagg gaggttttc ttcttcaag gacgaatac ttttggaga atcgacgaa accggcgtg ttaatgccg ggctaccct gcggagatc cgtcgcttg tggcgcgac cttccgccc aatttcacg cacgtggac gccgtctac gaacggccc gacaacaaa atcgttttt ttcatcgac gatcggttt tacgttttc accggaaat cgactcgag ccgggttac ccgcgcccc ttaaccgac ctggggctc cccgaaact ttgaaaaaa atcgacggg gcgatgatt tggggccac aacggcaag acctacttc tacagcggc tccatgtac tggagattc gacgaagag gagagaaaa gtcgagttg gattatcct agagatatg tccatgtgg agcggagtg gggacggac attgacgca gtcttccag tggaaagac gggagaacg tattttttc aaagggacc ggtttctgg aaattcaac gacatctac atgcgggtg gagcaccgg cagcagaag ccgtctggg cccttctgg atgggctgc agcaacaac ctggagggc aacgacgtc ctgcagaag ctgccctac aagcacttc gcagcgtct gggagttcg tgtcgctat ctggatgtg acagccgcc ttgcttgta attcttttg tattatcgg ttttaaaaa ctctcactg ccttattgg agaataata ctcaaagga aaaattcga agactgtaa gtaggaaga aaatgtgct cattgtgcg tgcactgcc tcaggaggt ttcctgaat ttgtgatag aggagcgtg tttttcggc attttattc atacttttt atttaagta tactgtgat attgttgaa attaaaacg attcattca aacacacat tttcggcta gcggtacaa atcatggta atttatgac tatttttat gtgtaaatt atttaatta ttgaatgta ggatcaggt ccgggatta aagcacaat atttttata tatttatat aataatgca aaaagtgcc attttcggc acagttgct gtttctgtc tgcctgacc tttacatta ttgttatgc tacgtctgt gtcgcggtg tgaacattg atcaaatat actgtaatt ttttatcta tcaactatg taataaaga acataaaac gag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
