Status
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Gene information
General information
TC006735 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13539378..13565746
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006735
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04205

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands not present - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006735 RA
      MCVCCGGGF LRVVLVLWL CGPSMHFRI SKREIVDDS 
VQRYLTRFG YMDATADGA FALRTEESV RDAIKDMQE 
FAGIPVTGR LDERTLKLL NTPRCGMPD KNKQTSGRR 
KRFTLHGQK WPYTNLTWS LRSRDLMGM DPYQVRLVI 
SKALDVWAR HSKLTFTEV DSPKADILI FFVRGEHGD 
NFPFDGKGV ILAHAFFPN GGHSIDVHF DADEAWTTV 
PNSDEGTNL FNVAAHEFG HSLGLSHSS EEGALMYPW 
YKEMENGFD YELPDDDKL GIQALYGAR TTRLWDRIK 
PYRPPTTTT TTTTTTTRR PYFRPSQRP FNPNGRHPH 
NPYNPYHPH KNNPKKPMH PDKKYHNPK EHPGRYPVH 
RYTTARPTP PPRFPDKYP DWRHHNSDK EGPKRPPDT 
CDTSYDAVA VIRREVFFF KDEYFWRID ETGVLMPGY 
PAEIRRLWR DLPPNFTHV DAVYERPDN KIVFFIDDR 
FYVFTGNRL EPGYPRPLT DLGLPETLK KIDGAMIWG 
HNGKTYFYS GSMYWRFDE EERKVELDY PRDMSMWSG 
VGTDIDAVF QWKDGRTYF FKGTGFWKF NDIYMRVEH 
RQQKPSGPF WMGCSNNLE GNDVLQKLP YKHFAASGS 
SCRYLDVTA ALLVILLYY RF
    CDS sequence
    • >TC006735 RA
      atgtgtgtg tgctgtggt ggtggtttc ctgcgggtg 
gttctagtg ctgtggctg tgcggacct tcgatgcac 
tttaggatt agcaaaagg gaaattgtc gatgattcg 
gtgcaacgc tacttgacc cgctttggc tacatggac 
gccacggca gatggcgct ttcgccctt cgaacggag 
gaatctgtg cgggacgcc attaaagac atgcaagag 
tttgcgggt attcccgta acggggcgc ttagatgaa 
cgaacactt aaattactc aatacgcct cgttgcggg 
atgcctgat aaaaataaa caaacatca ggcagaagg 
aaaagattc actttacac ggccagaaa tggccgtac 
acgaatcta acatggagc ttgaggagc cgggatttg 
atgggaatg gacccgtac caagtccgg ctcgtcatt 
tccaaagct ttggacgtt tgggcaaga cattccaaa 
cttaccttc acggaagtt gacagccct aaggcggat 
atactcatc tttttcgtt agaggcgaa cacggagac 
aatttcccc tttgacggc aaaggagtg attttggcg 
cacgctttt ttcccaaac ggtggccac agcatcgac 
gtccacttc gacgcagat gaggcctgg acaactgtg 
cccaacagc gatgaaggg actaattta ttcaatgtg 
gcggcccac gaattcggc cactccttg ggcctatcg 
cactcttcc gaagagggc gctctgatg tacccatgg 
tacaaagaa atggaaaat gggttcgac tacgaactc 
cccgacgac gacaaattg ggcattcag gccctttat 
ggtgccaga accacgcga ttgtgggac aggatcaag 
ccctacagg ccccccact accacaact acaaccacc 
acgaccacg acccgacgg ccctatttc aggcccagt 
caacgtcct tttaatccc aacgggcga catccacac 
aatccttac aatccctac catccacat aaaaacaat 
cccaaaaaa cccatgcat cccgataag aagtaccac 
aatcctaag gagcatcca gggaggtac ccggtgcat 
aggtacacg acggcgcga ccgacgcct ccgccgagg 
ttcccagat aagtacccc gactggagg caccataac 
agtgataag gagggcccc aaaaggccc ccagatacg 
tgcgatacg agctacgat gccgtggcg gtgattcgg 
agggaggtt ttcttcttc aaggacgaa tacttttgg 
agaatcgac gaaaccggc gtgttaatg ccgggctac 
cctgcggag atccgtcgc ttgtggcgc gaccttccg 
cccaatttc acgcacgtg gacgccgtc tacgaacgg 
cccgacaac aaaatcgtt tttttcatc gacgatcgg 
ttttacgtt ttcaccgga aatcgactc gagccgggt 
tacccgcgc cccttaacc gacctgggg ctccccgaa 
actttgaaa aaaatcgac ggggcgatg atttggggc 
cacaacggc aagacctac ttctacagc ggctccatg 
tactggaga ttcgacgaa gaggagaga aaagtcgag 
ttggattat cctagagat atgtccatg tggagcgga 
gtggggacg gacattgac gcagtcttc cagtggaaa 
gacgggaga acgtatttt ttcaaaggg accggtttc 
tggaaattc aacgacatc tacatgcgg gtggagcac 
cggcagcag aagccgtct gggcccttc tggatgggc 
tgcagcaac aacctggag ggcaacgac gtcctgcag 
aagctgccc tacaagcac ttcgcagcg tctgggagt 
tcgtgtcgc tatctggat gtgacagcc gccttgctt 
gtaattctt ttgtattat cggttttaa
    mRNA sequence
    • >TC006735 RA
      tagttcata aaaaacaac tctccattc tcgtgtagt 
cagtcctca gctggtgtt gctcggctc cgggtctct 
attttcgcc gtccagcct gaaataacg caccctctg 
aacacctcc gctcgctcc acagcatgg ccacgctgt 
gggaaatac gaatttcgg tttatcgga aaaacgtac 
gctgttgcg gtttctgta tttcctaac gcttgtgct 
tcctcggaa ccggagaaa tgtgtgttt gatataaac 
cggcgagtg gatgaaaac tggacattt tccgcgatg 
tgtgtgtgc tgtggtggt ggtttcctg cgggtggtt 
ctagtgctg tggctgtgc ggaccttcg atgcacttt 
aggattagc aaaagggaa attgtcgat gattcggtg 
caacgctac ttgacccgc tttggctac atggacgcc 
acggcagat ggcgctttc gcccttcga acggaggaa 
tctgtgcgg gacgccatt aaagacatg caagagttt 
gcgggtatt cccgtaacg gggcgctta gatgaacga 
acacttaaa ttactcaat acgcctcgt tgcgggatg 
cctgataaa aataaacaa acatcaggc agaaggaaa 
agattcact ttacacggc cagaaatgg ccgtacacg 
aatctaaca tggagcttg aggagccgg gatttgatg 
ggaatggac ccgtaccaa gtccggctc gtcatttcc 
aaagctttg gacgtttgg gcaagacat tccaaactt 
accttcacg gaagttgac agccctaag gcggatata 
ctcatcttt ttcgttaga ggcgaacac ggagacaat 
ttccccttt gacggcaaa ggagtgatt ttggcgcac 
gcttttttc ccaaacggt ggccacagc atcgacgtc 
cacttcgac gcagatgag gcctggaca actgtgccc 
aacagcgat gaagggact aatttattc aatgtggcg 
gcccacgaa ttcggccac tccttgggc ctatcgcac 
tcttccgaa gagggcgct ctgatgtac ccatggtac 
aaagaaatg gaaaatggg ttcgactac gaactcccc 
gacgacgac aaattgggc attcaggcc ctttatggt 
gccagaacc acgcgattg tgggacagg atcaagccc 
tacaggccc cccactacc acaactaca accaccacg 
accacgacc cgacggccc tatttcagg cccagtcaa 
cgtcctttt aatcccaac gggcgacat ccacacaat 
ccttacaat ccctaccat ccacataaa aacaatccc 
aaaaaaccc atgcatccc gataagaag taccacaat 
cctaaggag catccaggg aggtacccg gtgcatagg 
tacacgacg gcgcgaccg acgcctccg ccgaggttc 
ccagataag taccccgac tggaggcac cataacagt 
gataaggag ggccccaaa aggccccca gatacgtgc 
gatacgagc tacgatgcc gtggcggtg attcggagg 
gaggttttc ttcttcaag gacgaatac ttttggaga 
atcgacgaa accggcgtg ttaatgccg ggctaccct 
gcggagatc cgtcgcttg tggcgcgac cttccgccc 
aatttcacg cacgtggac gccgtctac gaacggccc 
gacaacaaa atcgttttt ttcatcgac gatcggttt 
tacgttttc accggaaat cgactcgag ccgggttac 
ccgcgcccc ttaaccgac ctggggctc cccgaaact 
ttgaaaaaa atcgacggg gcgatgatt tggggccac 
aacggcaag acctacttc tacagcggc tccatgtac 
tggagattc gacgaagag gagagaaaa gtcgagttg 
gattatcct agagatatg tccatgtgg agcggagtg 
gggacggac attgacgca gtcttccag tggaaagac 
gggagaacg tattttttc aaagggacc ggtttctgg 
aaattcaac gacatctac atgcgggtg gagcaccgg 
cagcagaag ccgtctggg cccttctgg atgggctgc 
agcaacaac ctggagggc aacgacgtc ctgcagaag 
ctgccctac aagcacttc gcagcgtct gggagttcg 
tgtcgctat ctggatgtg acagccgcc ttgcttgta 
attcttttg tattatcgg ttttaaaaa ctctcactg 
ccttattgg agaataata ctcaaagga aaaattcga 
agactgtaa gtaggaaga aaatgtgct cattgtgcg 
tgcactgcc tcaggaggt ttcctgaat ttgtgatag 
aggagcgtg tttttcggc attttattc atacttttt 
atttaagta tactgtgat attgttgaa attaaaacg 
attcattca aacacacat tttcggcta gcggtacaa 
atcatggta atttatgac tatttttat gtgtaaatt 
atttaatta ttgaatgta ggatcaggt ccgggatta 
aagcacaat atttttata tatttatat aataatgca 
aaaagtgcc attttcggc acagttgct gtttctgtc 
tgcctgacc tttacatta ttgttatgc tacgtctgt 
gtcgcggtg tgaacattg atcaaatat actgtaatt 
ttttatcta tcaactatg taataaaga acataaaac 
gag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY