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Gene information
General information
TC006726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13394429..13399335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006726
    iBeetle screen iB_04204

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    maxilla shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006726 RA
      MFRLILLLL YARRCLAYG YDSGGDQGA CGRQYRSGK 
      MHRPPCDLT SGYCTSAGT AYPWHAVKR FVRENQGLM 
      RRMYGDQRH GQVLQAELD DVELQSMTG EDRKMWAKQ 
      ESHFNTDNE LTRDTEPRF LREEDISND DVLNTKFVY 
      SEEPSISIK LADLRTAPH FRPTTTTTT EAPKTTTVT 
      EETTTVETA TTDTTDTTN DNNNPTEND SGDNNPQTV 
      LFQDMEDKN SHKVQVNYH MKGVNACPV KEEVVAPFW 
      ANNTRGEVL ALLNMYPFE QYVHWEKCT HEHRQMYCR 
      DGCRCEQQY RLHRLLAYD PNNECRGIF SDWFRFPSC 
      CVCKCYSIP PAEFRVTSR SPRAFDDGQ VPPDWYRRK 
      NEAIFGGDH G
    CDS sequence
    • >TC006726 RA
      atgttccgc ctaattcta ctcctttta tacgcccgt 
      cgatgtctt gcctacgga tacgattca gggggcgac 
      cagggggct tgtgggcgc caataccgc tcgggaaaa 
      atgcacaga cctccgtgc gatttaacc tcaggctat 
      tgcacctca gctggcact gcctacccc tggcacgct 
      gtgaagagg tttgtgagg gagaatcaa ggcctgatg 
      aggcggatg tacggggat cagaggcat ggacaggtg 
      ctccaggcc gagctggac gacgtggag ctgcagtca 
      atgacaggg gaggataga aaaatgtgg gctaagcaa 
      gaatcccac ttcaacacc gacaacgag ctaacgcga 
      gacactgag ccccgattt ctgcgtgag gaagatata 
      agtaatgat gacgttctc aacaccaaa ttcgtttat 
      tcagaggag ccttcaatt agtataaaa ttggcagat 
      ttgcggacg gctcctcat ttcagaccc acaacaaca 
      accacaacc gaagcacca aaaacaaca accgtaact 
      gaagaaaca accacagtt gaaaccgca accactgac 
      actactgat acaactaat gataataat aatcccact 
      gaaaatgat tcaggtgat aacaatccc caaacggtc 
      cttttccag gacatggag gacaaaaac agtcacaag 
      gtgcaagtc aactaccac atgaagggg gtgaatgct 
      tgtccggtg aaagaagaa gtggtggcc cccttctgg 
      gcgaataac acccggggc gaagtccta gccttgctt 
      aatatgtac ccctttgaa cagtacgtc cactgggag 
      aagtgcacc cacgagcac cgccagatg tactgtcga 
      gatgggtgt cgctgtgag cagcagtat cgtctccat 
      agactgctg gcctacgac cccaataat gaatgtcgg 
      gggattttc tcggattgg tttcgattt ccgtcgtgt 
      tgtgtttgt aaatgttac agtatacca ccggcggag 
      tttcgtgtg acgtcgcga agtccacga gcttttgac 
      gatgggcaa gtgcccccc gattggtat cgaagaaaa 
      aatgaggct atttttgga ggtgatcat ggatga
    mRNA sequence
    • >TC006726 RA
      ttacggtta agagcatct gacagtaga aacctcatt 
      tctcgcgta aacaggagg gtctcggct ttggaacag 
      ttcaccggt acctcgctg tcggtcaac agctcttgt 
      tcttcagtt acaaaaaaa aaaactcgc gcgaccatg 
      cggccaatt cggtgcctt tctgtgcaa atcgctggt 
      ttggaccaa gtgtagaga ctcaaatgc gagatgttc 
      cgcctaatt ctactcctt ttatacgcc cgtcgatgt 
      cttgcctac ggatacgat tcagggggc gaccagggg 
      gcttgtggg cgccaatac cgctcggga aaaatgcac 
      agacctccg tgcgattta acctcaggc tattgcacc 
      tcagctggc actgcctac ccctggcac gctgtgaag 
      aggtttgtg agggagaat caaggcctg atgaggcgg 
      atgtacggg gatcagagg catggacag gtgctccag 
      gccgagctg gacgacgtg gagctgcag tcaatgaca 
      ggggaggat agaaaaatg tgggctaag caagaatcc 
      cacttcaac accgacaac gagctaacg cgagacact 
      gagccccga tttctgcgt gaggaagat ataagtaat 
      gatgacgtt ctcaacacc aaattcgtt tattcagag 
      gagccttca attagtata aaattggca gatttgcgg 
      acggctcct catttcaga cccacaaca acaaccaca 
      accgaagca ccaaaaaca acaaccgta actgaagaa 
      acaaccaca gttgaaacc gcaaccact gacactact 
      gatacaact aatgataat aataatccc actgaaaat 
      gattcaggt gataacaat ccccaaacg gtccttttc 
      caggacatg gaggacaaa aacagtcac aaggtgcaa 
      gtcaactac cacatgaag ggggtgaat gcttgtccg 
      gtgaaagaa gaagtggtg gcccccttc tgggcgaat 
      aacacccgg ggcgaagtc ctagccttg cttaatatg 
      taccccttt gaacagtac gtccactgg gagaagtgc 
      acccacgag caccgccag atgtactgt cgagatggg 
      tgtcgctgt gagcagcag tatcgtctc catagactg 
      ctggcctac gaccccaat aatgaatgt cgggggatt 
      ttctcggat tggtttcga tttccgtcg tgttgtgtt 
      tgtaaatgt tacagtata ccaccggcg gagtttcgt 
      gtgacgtcg cgaagtcca cgagctttt gacgatggg 
      caagtgccc cccgattgg tatcgaaga aaaaatgag 
      gctattttt ggaggtgat catggatga ttcgaaaat 
      tggaaaagt aatcgccaa ggctcgtgt tttgtaatt 
      tagttattt ttgtgtcta tgttttgta catttatca 
      ttaatacta ttaattgta tgttgtagt tggtaagac 
      ttgttgtgt tcttgtaat gtgttaatt tttttataa 
      aattacgaa taaagttta tgattaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY