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Gene information
General information
TC006723 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13356478..13364093
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006723
      iBeetle screen iB_04203

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      larva circumference decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      labium split - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      legs pretarsus rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen circumference decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen potentially constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006723 RA
        MSPASIIVL ITVASCVFS YPSKPLNER 
        STELAWEAW LLVDQSNRQ SDLGMLRRR 
        ITPKSVFIA PTFSPDSLP ACAEGYKSD 
        VMGRCVKII KINESVHFD FLLQKLNAQ 
        FPDYEEVVE ENTTHGPLQ LNIPLFGDE 
        DKDVAIVVT PSKKNHHDT TTTVKVDVK 
        RNGGDKFGV EDVAAILGV KPEEVEVTT 
        IADFATTIK DVESTTLEG TTTTESAST 
        TSTEGGETT TLLDGEALF WRRI
      CDS sequence
      • >TC006723 RA
        atgagcccc gcatccata attgtgtta atcaccgtt 
        gcatcttgc gtcttcagc tacccctcc aagcccttg 
        aacgagcgt tccaccgaa ctggcttgg gaggcttgg 
        ctgctggtc gaccagagc aaccgtcag agcgacttg 
        gggatgctc cgacggaga atcaccccc aagtcggtc 
        ttcatcgcc cccaccttc agccccgac tcgctgccg 
        gcctgcgcc gaaggctac aagtcggac gtgatgggg 
        cgctgcgtg aaaatcatc aaaatcaac gagtcggtg 
        cactttgac ttcctcctc cagaagctc aacgcgcag 
        ttcccggac tacgaggaa gtcgttgag gagaatacc 
        acgcatggg cccctccag ctcaacatc ccgctcttt 
        ggggacgag gacaaggac gtggcgatt gtggtcact 
        ccctccaag aagaaccac catgatacc acgacaacg 
        gtcaaggtg gacgtgaag cggaatggg ggtgataag 
        ttcggggtt gaggatgtt gctgcgatt ttgggggtg 
        aagcccgag gaggtggaa gtgaccacg atcgccgat 
        ttcgccaca accatcaaa gatgtggaa agtaccacg 
        cttgagggt actaccact acggagagt gcgtccacc 
        accagtaca gaaggtggg gagacgacg acgctcctc 
        gatggtgag gcgctcttc tggaggagg atttaa
      mRNA sequence
      • >TC006723 RA
        ctacgcttc aggaatcgt aatgaaaaa tattgactg 
        gcgaagtgt aggcgtgta gcaaatcta gtgaaacag 
        tccgttgtt tgttgcaaa tttaatcgc aaacctata 
        acttttcaa gtgataagt gagtgtttt ccgcagttc 
        gtaaatcaa gtgcgtcac aaaaattgc ccattttcg 
        ccgataaac agtcgtaaa cccagctaa gaattttcc 
        gcgccatca gataagcca ccacttctt gtggaaaag 
        ctaagggtt cgccccact cgagtgcac ctgttcgtg 
        ttggtgtgt cttgcagtc gtcagagcg ttatatacc 
        ggtgaggtc gccgcaact ccccagtca aacgcttga 
        aaccgctgt gttcgcgcg agtttcctt caccaagtg 
        tcttttgca cccccggag tgcccaagt tcgtgccag 
        ggcgattct gccgcagtg tgtgtcaaa taagattcc 
        gatgatgtg gtgccccag tcgcaacac ttagcccgt 
        ccgaagatg agcaaaacg ccaccatca tgagccccg 
        catccataa ttgtgttaa tcaccgttg catcttgcg 
        tcttcagct acccctcca agcccttga acgagcgtt 
        ccaccgaac tggcttggg aggcttggc tgctggtcg 
        accagagca accgtcaga gcgacttgg ggatgctcc 
        gacggagaa tcaccccca agtcggtct tcatcgccc 
        ccaccttca gccccgact cgctgccgg cctgcgccg 
        aaggctaca agtcggacg tgatggggc gctgcgtga 
        aaatcatca aaatcaacg agtcggtgc actttgact 
        tcctcctcc agaagctca acgcgcagt tcccggact 
        acgaggaag tcgttgagg agaatacca cgcatgggc 
        ccctccagc tcaacatcc cgctctttg gggacgagg 
        acaaggacg tggcgattg tggtcactc cctccaaga 
        agaaccacc atgatacca cgacaacgg tcaaggtgg 
        acgtgaagc ggaatgggg gtgataagt tcggggttg 
        aggatgttg ctgcgattt tgggggtga agcccgagg 
        aggtggaag tgaccacga tcgccgatt tcgccacaa 
        ccatcaaag atgtggaaa gtaccacgc ttgagggta 
        ctaccacta cggagagtg cgtccacca ccagtacag 
        aaggtgggg agacgacga cgctcctcg atggtgagg 
        cgctcttct ggaggagga tttaaggac tgtgaggct 
        ccaagttgc cacttggag tgaactgct actaaacca 
        aatcacgta caaaaaaca gtaagacaa taaaagaac 
        acgcccaga gtcacaccc tacacatgg gcgccgact 
        aacaacgaa ccaggcaag tggtcggcg cctgtgctg 
        gtcctagtc tagggttca aaccaaatc gctattgtg 
        attctttta gtctttaac tacggtatt acttgtaca 
        tagtgtgta aatggaggt gcaaatgtt ttaatttgt 
        cgtttcttg tacatatta ttatttatt ttactaacg 
        cgggagtga gcttgacca cagtattac acactccga 
        tttactaat cttatcaac tgtattgta ggactaggt 
        taatcgtgc aaatgtaac gtatcgtat tgtttttca 
        acacacaaa cactagact gattaacct tttgttcga 
        ctgttaggt acaaatgta aagttagat taagctgaa 
        aagtgtatt tattatcag aatattcca tgttatgtt 
        cttgtgcca gtatacaaa taaatttac aagcagact
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY