Status
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Gene information
General information
TC006721 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13347678..13353912
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006721
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04202

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006721 RA
      MSEEKAKRS GRIPVRVKP GTRKNQTVA TGTKPGKEK 
      WVPKSNKPS RIPVLVKRR PKKIIRNKS YTKVKKAPP 
      QKPEPKLNE ESGGKNHWH ERGDFGDIR RKSSVTLKK 
      SSIIVREKG EEIPQIIFN EEFEETDTL SSSTLSLNS 
      EELVFFSRI EEMSSCEFE TIFDSQLEA LDDFDLMPL 
      QPEYAFEKE EEEKSHSVS EIQNADYFR CKNYLQSSF 
      PFIVMAQFD TKTPPLPPP QSEGVLIKL EEEEEEFEY 
      EPEWKGSET SIVIQRYID SGLRPLKPD ESRLYLDPI 
      HYQRKKKVP TTYDGVQPL LQDIPSEIF QTPKVRKTE 
      SPQKVGPES EVLSEKESE SSEESLEIP QTPESIDTE 
      PLKKLQELE DTTFTIETD KEEEEPEQL EEKEEEPED 
      LEEKEEEPE IIEEQEEEP EIIEEKEEE PEIMEKKEE 
      EPEVIEEKE EEPEVIEEK EEEPEILEE KEEEPEILE 
      EKEEEPEIL EEEEEEPEI VEEKEEEPE IIEKKEEEP 
      EVLEEKEEE PEIVEEKEE EPEIIEKKE EEPEVLEET 
      EEEKEPEIL KEKEEEKEP EILEEKEEE PEPEIIEEK 
      AEEPEIIEE KEEEPEILE EKEEEPEIV EEKEEEPEI 
      LEEKEEEPE IVEKKEEPE IVEEKEEPE ILEEKQEEK 
      EPEVLEEKK EEPKIIEKK EEEPEIIEK KKEEPEIIE 
      IKKEEPEIL EEKEEEPEI LEEKEEEPK ILEEKEEEP 
      EIIKDKEEE PEIIEEKKE SVIIEEKEE EPAIVEEKE 
      EEPEITEEE EEESEIIMI KEEEPEIIE EKEEEPEII 
      ETKGEPEII EEKEEEPEI VEKKEEKPE EPFEYKPKE 
      LKKREQDEF CIHTRDPPY EGDSGEFSS TSTLTDEVI 
      EWKERKQRT IFKIIRIKD EFCVHTHSP VSSYSDSSC 
      SCANLTEEE EVPKTKIRT SPQPKFDQK VKSRSRRTK 
      NLEKGDQNT KKLTEEKSK DQQIQTSST SGSLTEKNK 
      IKTRDAASL ATTESLIKY IATGSQTKS TQTVDAKSL 
      DSCECITEY KKSRKQSTS DKSLQVQIR TSPSNSLAS 
      RESLTKKQA SSKSGSTSD RGLKVQSRT SPRESMAEE 
      SHTQTTDAE SLASRESIT KDKSKGSRS RSTSDKNFT 
      VQSRTSRLG SLAEEDQIQ TADAESFVS RESVTKDKS 
      TGSRSRSTS NKVLKVQSR TSPTGSLAE ETAAAESLV 
      SRGSLTKDK ISSRSGSMS VKSLKIPSR TSPRSSLAE 
      EGQIQTAEA GSLALRGSI TKDKPTGSR SRSTSNKVL 
      KVQSRTSPT GSLTEGTAA AESLVSRGS LTKDKISSR 
      SGSMSVKSR TSPRSSLTE EGQIQTADA RSLASRGSI 
      TKKKPTSSR SKSTSDKNF KAQSRTSPT GSLTEEGQT 
      KTGDAGSLA SRVTITKDK TTGSRSKST SDKSIKVQS 
      RTSPTGSLA EEDQTKTSD AESLVSRES ITKDKLTSS 
      RSKSTSDKT LKVQSGTSP PESSDEEGQ TNTADARSL 
      ASRGSLTKD KTTSSRSRS TSIKSFKVQ TRTSPLSIL 
      AEEGEIQTA EAESLASRG SISKKKGSQ SVTTSDKSM 
      KVLSRPSPP GSLAEEGQT KTDDAGSLV SRESITKEK 
      GSRSGSTSD KNLKVQTRT SPPGSLAEE GQTKTGDAK 
      SSASRGSIT KDKTTSSRS RSTSVKSFK VQTRTSPLS 
      SLAEEQTAE VKSLASRGS ITKEKGSRS GSMSDKGPK 
      VQTRTSLPE SLTEEGQTK TSEAESLAS RGSITKEQT 
      TKSRSESMS DKGLKVQTR TSPPGNLPE KDQTQNASS 
      TKSITKDKP TGSRAENIP EKGLKVQIR SSRLSSLAE 
      EGQTQTADG KTLASTKSI IRDKSPSSR TGSISEKSL 
      KVQIRASPP GSLSEEDQT PISDKPATV GKKNKRERR 
      PKVRHQPST RSLVEPEKD SAISASTLM LVSNKERRR 
      TKSRMQVLS KLP
    CDS sequence
    • >TC006721 RA
      atgagcgag gaaaaagca aaacgctcc ggacgaatt 
      ccggtgcgt gttaaacca ggaacgcga aaaaatcaa 
      acagttgca accggaact aaacccgga aaagaaaaa 
      tgggtgcca aagagcaac aaaccttcc agaattcca 
      gttttggta aaaaggcgc ccgaaaaaa atcattagg 
      aacaagtca tacaccaaa gttaaaaaa gcgccacca 
      caaaaacca gaaccaaaa ttgaatgaa gaaagtggt 
      ggaaaaaac cactggcac gaacgtgga gattttgga 
      gacatacgt cgcaagtct agtgttact ttgaaaaaa 
      tcatccatt atagttcga gaaaagggc gaagaaatc 
      ccccagata atttttaat gaagaattc gaagaaacc 
      gatacttta agctcaagc acgctgtct ttgaacagt 
      gaagaacta gtgtttttt agtcgcatc gaggaaatg 
      agtagttgc gaattcgag actattttc gatagtcaa 
      cttgaagca ttggacgat tttgactta atgccactt 
      cagcccgag tacgctttc gaaaaagaa gaagaagaa 
      aaaagtcac tctgtttcc gaaattcag aatgctgat 
      tatttccgc tgtaaaaac tacctccaa tcgtccttc 
      ccctttata gtcatggcc caattcgac accaaaact 
      cctcccctt cctccccct caatcagaa ggggtctta 
      ataaagctc gaagaagaa gaagaagaa tttgaatat 
      gagccagaa tggaaaggc agcgaaaca agtatcgta 
      attcaacgc tacattgat tcgggttta aggccttta 
      aagccggac gagtcacgt ctatatctt gatccaatc 
      cattatcaa agaaagaag aaagttcca acgacttac 
      gatggagtc caaccactt ttacaagac attccgtcc 
      gaaatcttt caaactccg aaagttcgt aaaacggag 
      agtccacaa aaagtggga cccgaatca gaagttttg 
      agcgaaaaa gaatcagaa tcaagtgaa gaaagtctg 
      gaaatacca caaactccc gaaagtatt gacactgaa 
      ccgttgaag aagttgcaa gagctcgaa gatacgact 
      ttcaccatc gaaactgac aaggaagaa gaagagccc 
      gaacaattg gaagagaaa gaagaagag cctgaagat 
      ttagaagag aaggaagaa gaacccgaa attatagag 
      gaacaggaa gaagaaccg gaaatcata gaggaaaaa 
      gaagaagag cctgaaatt atggagaaa aaagaagaa 
      gagcctgaa gttatagag gagaaggaa gaagagcct 
      gaagttata gaggagaaa gaagaagag cctgaaatt 
      ttagaggag aaggaagaa gagcctgaa attttagag 
      gagaaggaa gaagagcct gaaatttta gaggaggaa 
      gaagaagag cctgaaatt gtggaggag aaagaagaa 
      gagcctgaa attatagag aagaaggaa gaagagcct 
      gaagttttg gaggagaaa gaagaagag cctgaaatt 
      gtggaggag aaagaagaa gagcctgaa attatagag 
      aagaaagaa gaagagcct gaagtttta gaagagacg 
      gaagaagaa aaagagcct gaaatttta aaagagaag 
      gaagaagaa aaagaacct gaaatttta gaagagaag 
      gaagaagaa cctgagcct gaaatcata gaagagaaa 
      gcagaagag cctgaaatt atagaggag aaggaagaa 
      gagcctgaa attttagag gagaaagaa gaagagcct 
      gaaattgtg gaggagaaa gaagaagag cctgaaatt 
      ttagaggag aaagaagaa gagcctgaa attgtagag 
      aagaaagaa gagcctgaa attgtagag gagaaagaa 
      gagcctgaa attttagag gagaagcaa gaagaaaaa 
      gagcctgaa gttttagaa gagaagaaa gaagagcct 
      aaaatcata gagaagaaa gaagaagaa cctgaaatc 
      atagagaag aaaaaagaa gagcctgaa attatagaa 
      ataaaaaaa gaagagcct gaaatttta gaggaaaaa 
      gaagaagag cctgaaatt ttagaagag aaagaagag 
      gagcctaaa attttagag gagaaagaa gaagagcca 
      gaaatcata aaggataag gaagaggag cctgaaatc 
      attgaggag aaaaaagag tcagtaatc atagaggag 
      aaggaagaa gagcctgca attgtggag gagaaagaa 
      gaggagcct gaaatcaca gaagaggag gaagaagag 
      tctgaaatc ataatgata aaagaagaa gagcctgaa 
      atcatagaa gagaaggaa gaagagcct gaaatcata 
      gagacgaaa ggagagcct gaaattata gaagagaag 
      gaagaagag cctgaaatt gtggagaag aaagaagaa 
      aagcctgaa gaacctttt gaatataag cctaaagag 
      ttgaaaaaa agagaacaa gacgagttt tgcattcat 
      acaagagat cccccttat gaaggcgat tcaggagaa 
      ttttcctcc acttcgact ctaacagat gaagtaata 
      gagtggaag gaaagaaaa caacgaacc atcttcaaa 
      atcataaga ataaaagac gagttttgt gttcacact 
      cacagccct gtgtctagt tatagtgac agtagttgc 
      tcatgtgcg aatttaacc gaagaagaa gaagtccct 
      aaaaccaaa attcggact tcaccacaa ccgaaattt 
      gaccaaaaa gtcaaaagt cggtctcga cgaacaaaa 
      aatttagaa aaaggagac caaaatacc aaaaagtta 
      acagaagaa aaatctaaa gaccaacaa attcagact 
      tcttcaaca agtggcagt ctgaccgaa aaaaacaaa 
      attaaaact agggatgct gcaagtttg gccacaact 
      gaaagttta ataaaatac atagcaaca ggttctcaa 
      acaaaaagt acccaaact gtcgatgcg aaaagtttg 
      gattcttgc gaatgtata acagagtac aaaaaatcc 
      agaaaacaa agtacgtcg gataaaagc ctccaagtc 
      cagattcgg acttcacct tctaatagt ttggcctca 
      cgtgaaagt ctgacaaag aaacaagca agttccaaa 
      agtggaagt acatcagat agaggtctt aaagtccaa 
      agtaggact tcacctcgc gaaagtatg gccgaagaa 
      agccataca caaacaact gatgccgaa agtttggct 
      tcacgtgaa agtataaca aaagacaaa tcaaaaggt 
      tcccgaagt agaagtacg tcggataaa aattttaca 
      gtccaaagt aggacttca cgtctcggg agtttggct 
      gaagaagac caaatacaa actgctgat gcagaaagt 
      ttcgtctca cgtgaaagt gtaacaaaa gacaaatca 
      acaggttcc cgaagtaga agtacatca aataaagtt 
      cttaaagtc caaagtagg acttcacct accggaagt 
      ttggccgaa gaaactgct gctgccgaa agtttagtc 
      tcacgtgga agcttaaca aaagacaaa ataagttcc 
      cgaagtgga agtatgtcg gttaaaagt cttaaaatc 
      ccaagtcgg acttcgcct cgaagcagt ttggccgaa 
      gaaggccaa atacaaact gctgaagcc ggaagttta 
      gctttacgt ggaagtata acaaaggac aaaccaaca 
      ggttcccga agtagaagt acatcaaat aaagttctt 
      aaagtccaa agtaggact tcacctact gggagtttg 
      accgaagga actgctgct gccgaaagt ttagtctca 
      cgtggaagc ttaacaaaa gacaaaata agttcccga 
      agtggaagt atgtcggtt aaaagtcgg acttcgcct 
      cgaagcagt ttgaccgaa gaaggccaa atacaaact 
      gctgacgcc agaagttta gcttcacgt ggaagtata 
      acaaagaag aaaccaaca agttcccga agtaaaagt 
      acgtcggat aaaaatttt aaagcccaa agtaggact 
      tcacctacc gggagtttg accgaagaa ggtcaaact 
      aaaactggt gatgctgga agtttggcc tcacgagta 
      actataaca aaggacaaa acaacaggt tcccgaagt 
      aaaagtacg tcagataaa agtattaaa gtgcaaagt 
      aggacttca cctaccggg agtttggcc gaagaagac 
      caaactaaa acttctgat gccgaaagt ttagtctca 
      cgtgaaagc ataacaaaa gacaaatta acaagttcc 
      cgaagtaaa agtacgtca gataaaact cttaaagtc 
      cagagtggg acttcacct cccgagagt tcggacgaa 
      gaaggacaa actaatact gctgatgcc agaagtttg 
      gcttcacgt ggtagttta acaaaagac aaaacaacc 
      agttcccga agtagaagt acgtcaatt aaaagtttt 
      aaagtccaa actcggact tcgcctcta agcattttg 
      gctgaagaa ggcgaaata caaacagcc gaagccgaa 
      agtttggcg tcacgtgga agtatatca aagaaaaaa 
      ggttcccaa agtgtaact acgtcagat aagagcatg 
      aaagtccta agtcggcct tcaccaccc ggaagtttg 
      gccgaagaa ggccaaact aaaaccgat gatgccgga 
      agtttggtc tcacgtgaa agtataaca aaagaaaaa 
      ggttcccga agtggaagt acgtcggat aagaacctc 
      aaagtccaa actcggact tcacccccc ggaagttta 
      gctgaagaa ggccaaact aaaactggt gatgccaaa 
      agttcggct tcacgtgga agtataaca aaggacaaa 
      acaacaagt tcccgaagt agaagtacg tcggttaaa 
      agttttaaa gtccaaact cggacttca cctctaagc 
      agtttggcg gaggaacaa actgccgaa gtcaaaagt 
      ttagcttca cgtggaagt ataacaaag gaaaaaggt 
      tcccgaagt ggaagtatg tcagataaa ggccccaaa 
      gtccaaact cggacttca cttcccgag agtttgact 
      gaagaaggc caaactaaa actagtgaa gccgaaagt 
      ttggcctca cgtggaagt ataacaaaa gaacaaaca 
      acaaaatcc cgaagtgaa agtatgtcg gataaaggt 
      ctgaaagtc caaactagg acttcacct cctggcaat 
      ttgcccgaa aaagatcaa actcaaaat gcttcttct 
      actaaaagt ataacaaaa gacaagcca acaggttcc 
      cgtgcagaa aatatccca gaaaaaggc ctcaaggtt 
      caaattcgg tcttcacgc ctaagcagt ttggccgaa 
      gaaggccaa actcaaaca gccgatggt aaaactcta 
      gcctctacg aaaagtata ataagagat aagtcacca 
      agttcccga acaggaagt atctcagaa aagagccta 
      aaagtccaa attcgggct tcacctccc ggtagtttg 
      tcagaagaa gaccaaact ccaatttcc gataaacca 
      gcaactgtt ggtaaaaag aataaacga gaacggcgt 
      ccaaaagtt cgtcatcaa ccttcaacg agatcgctt 
      gtagaaccc gaaaaagat tcggcaatt tcggcttca 
      accttaatg ctcgtcagt aataaagaa cgaagacgt 
      actaaaagt cgcatgcaa gtcctttca aaattacct 
      tga
    mRNA sequence
    • >TC006721 RA
      cagttaaca aaactatcg ttttcctcg cattgttgc 
      ttttttgac attctgcca cacgatttg acactattt 
      tgtcaacga attaaatct ccgtacgtc taaaattac 
      cgctgtgtg ccctatcct tctatttat gctcctaag 
      caaatcgat tggcttcgc tgaacccaa attaaacca 
      aatttcaac ggaaatctt cacttagaa tttcgcata 
      gtcacgttc gagaaaaaa acgtttaac ggtaaaatt 
      atgagcgag gaaaaagca aaacgctcc ggacgaatt 
      ccggtgcgt gttaaacca ggaacgcga aaaaatcaa 
      acagttgca accggaact aaacccgga aaagaaaaa 
      tgggtgcca aagagcaac aaaccttcc agaattcca 
      gttttggta aaaaggcgc ccgaaaaaa atcattagg 
      aacaagtca tacaccaaa gttaaaaaa gcgccacca 
      caaaaacca gaaccaaaa ttgaatgaa gaaagtggt 
      ggaaaaaac cactggcac gaacgtgga gattttgga 
      gacatacgt cgcaagtct agtgttact ttgaaaaaa 
      tcatccatt atagttcga gaaaagggc gaagaaatc 
      ccccagata atttttaat gaagaattc gaagaaacc 
      gatacttta agctcaagc acgctgtct ttgaacagt 
      gaagaacta gtgtttttt agtcgcatc gaggaaatg 
      agtagttgc gaattcgag actattttc gatagtcaa 
      cttgaagca ttggacgat tttgactta atgccactt 
      cagcccgag tacgctttc gaaaaagaa gaagaagaa 
      aaaagtcac tctgtttcc gaaattcag aatgctgat 
      tatttccgc tgtaaaaac tacctccaa tcgtccttc 
      ccctttata gtcatggcc caattcgac accaaaact 
      cctcccctt cctccccct caatcagaa ggggtctta 
      ataaagctc gaagaagaa gaagaagaa tttgaatat 
      gagccagaa tggaaaggc agcgaaaca agtatcgta 
      attcaacgc tacattgat tcgggttta aggccttta 
      aagccggac gagtcacgt ctatatctt gatccaatc 
      cattatcaa agaaagaag aaagttcca acgacttac 
      gatggagtc caaccactt ttacaagac attccgtcc 
      gaaatcttt caaactccg aaagttcgt aaaacggag 
      agtccacaa aaagtggga cccgaatca gaagttttg 
      agcgaaaaa gaatcagaa tcaagtgaa gaaagtctg 
      gaaatacca caaactccc gaaagtatt gacactgaa 
      ccgttgaag aagttgcaa gagctcgaa gatacgact 
      ttcaccatc gaaactgac aaggaagaa gaagagccc 
      gaacaattg gaagagaaa gaagaagag cctgaagat 
      ttagaagag aaggaagaa gaacccgaa attatagag 
      gaacaggaa gaagaaccg gaaatcata gaggaaaaa 
      gaagaagag cctgaaatt atggagaaa aaagaagaa 
      gagcctgaa gttatagag gagaaggaa gaagagcct 
      gaagttata gaggagaaa gaagaagag cctgaaatt 
      ttagaggag aaggaagaa gagcctgaa attttagag 
      gagaaggaa gaagagcct gaaatttta gaggaggaa 
      gaagaagag cctgaaatt gtggaggag aaagaagaa 
      gagcctgaa attatagag aagaaggaa gaagagcct 
      gaagttttg gaggagaaa gaagaagag cctgaaatt 
      gtggaggag aaagaagaa gagcctgaa attatagag 
      aagaaagaa gaagagcct gaagtttta gaagagacg 
      gaagaagaa aaagagcct gaaatttta aaagagaag 
      gaagaagaa aaagaacct gaaatttta gaagagaag 
      gaagaagaa cctgagcct gaaatcata gaagagaaa 
      gcagaagag cctgaaatt atagaggag aaggaagaa 
      gagcctgaa attttagag gagaaagaa gaagagcct 
      gaaattgtg gaggagaaa gaagaagag cctgaaatt 
      ttagaggag aaagaagaa gagcctgaa attgtagag 
      aagaaagaa gagcctgaa attgtagag gagaaagaa 
      gagcctgaa attttagag gagaagcaa gaagaaaaa 
      gagcctgaa gttttagaa gagaagaaa gaagagcct 
      aaaatcata gagaagaaa gaagaagaa cctgaaatc 
      atagagaag aaaaaagaa gagcctgaa attatagaa 
      ataaaaaaa gaagagcct gaaatttta gaggaaaaa 
      gaagaagag cctgaaatt ttagaagag aaagaagag 
      gagcctaaa attttagag gagaaagaa gaagagcca 
      gaaatcata aaggataag gaagaggag cctgaaatc 
      attgaggag aaaaaagag tcagtaatc atagaggag 
      aaggaagaa gagcctgca attgtggag gagaaagaa 
      gaggagcct gaaatcaca gaagaggag gaagaagag 
      tctgaaatc ataatgata aaagaagaa gagcctgaa 
      atcatagaa gagaaggaa gaagagcct gaaatcata 
      gagacgaaa ggagagcct gaaattata gaagagaag 
      gaagaagag cctgaaatt gtggagaag aaagaagaa 
      aagcctgaa gaacctttt gaatataag cctaaagag 
      ttgaaaaaa agagaacaa gacgagttt tgcattcat 
      acaagagat cccccttat gaaggcgat tcaggagaa 
      ttttcctcc acttcgact ctaacagat gaagtaata 
      gagtggaag gaaagaaaa caacgaacc atcttcaaa 
      atcataaga ataaaagac gagttttgt gttcacact 
      cacagccct gtgtctagt tatagtgac agtagttgc 
      tcatgtgcg aatttaacc gaagaagaa gaagtccct 
      aaaaccaaa attcggact tcaccacaa ccgaaattt 
      gaccaaaaa gtcaaaagt cggtctcga cgaacaaaa 
      aatttagaa aaaggagac caaaatacc aaaaagtta 
      acagaagaa aaatctaaa gaccaacaa attcagact 
      tcttcaaca agtggcagt ctgaccgaa aaaaacaaa 
      attaaaact agggatgct gcaagtttg gccacaact 
      gaaagttta ataaaatac atagcaaca ggttctcaa 
      acaaaaagt acccaaact gtcgatgcg aaaagtttg 
      gattcttgc gaatgtata acagagtac aaaaaatcc 
      agaaaacaa agtacgtcg gataaaagc ctccaagtc 
      cagattcgg acttcacct tctaatagt ttggcctca 
      cgtgaaagt ctgacaaag aaacaagca agttccaaa 
      agtggaagt acatcagat agaggtctt aaagtccaa 
      agtaggact tcacctcgc gaaagtatg gccgaagaa 
      agccataca caaacaact gatgccgaa agtttggct 
      tcacgtgaa agtataaca aaagacaaa tcaaaaggt 
      tcccgaagt agaagtacg tcggataaa aattttaca 
      gtccaaagt aggacttca cgtctcggg agtttggct 
      gaagaagac caaatacaa actgctgat gcagaaagt 
      ttcgtctca cgtgaaagt gtaacaaaa gacaaatca 
      acaggttcc cgaagtaga agtacatca aataaagtt 
      cttaaagtc caaagtagg acttcacct accggaagt 
      ttggccgaa gaaactgct gctgccgaa agtttagtc 
      tcacgtgga agcttaaca aaagacaaa ataagttcc 
      cgaagtgga agtatgtcg gttaaaagt cttaaaatc 
      ccaagtcgg acttcgcct cgaagcagt ttggccgaa 
      gaaggccaa atacaaact gctgaagcc ggaagttta 
      gctttacgt ggaagtata acaaaggac aaaccaaca 
      ggttcccga agtagaagt acatcaaat aaagttctt 
      aaagtccaa agtaggact tcacctact gggagtttg 
      accgaagga actgctgct gccgaaagt ttagtctca 
      cgtggaagc ttaacaaaa gacaaaata agttcccga 
      agtggaagt atgtcggtt aaaagtcgg acttcgcct 
      cgaagcagt ttgaccgaa gaaggccaa atacaaact 
      gctgacgcc agaagttta gcttcacgt ggaagtata 
      acaaagaag aaaccaaca agttcccga agtaaaagt 
      acgtcggat aaaaatttt aaagcccaa agtaggact 
      tcacctacc gggagtttg accgaagaa ggtcaaact 
      aaaactggt gatgctgga agtttggcc tcacgagta 
      actataaca aaggacaaa acaacaggt tcccgaagt 
      aaaagtacg tcagataaa agtattaaa gtgcaaagt 
      aggacttca cctaccggg agtttggcc gaagaagac 
      caaactaaa acttctgat gccgaaagt ttagtctca 
      cgtgaaagc ataacaaaa gacaaatta acaagttcc 
      cgaagtaaa agtacgtca gataaaact cttaaagtc 
      cagagtggg acttcacct cccgagagt tcggacgaa 
      gaaggacaa actaatact gctgatgcc agaagtttg 
      gcttcacgt ggtagttta acaaaagac aaaacaacc 
      agttcccga agtagaagt acgtcaatt aaaagtttt 
      aaagtccaa actcggact tcgcctcta agcattttg 
      gctgaagaa ggcgaaata caaacagcc gaagccgaa 
      agtttggcg tcacgtgga agtatatca aagaaaaaa 
      ggttcccaa agtgtaact acgtcagat aagagcatg 
      aaagtccta agtcggcct tcaccaccc ggaagtttg 
      gccgaagaa ggccaaact aaaaccgat gatgccgga 
      agtttggtc tcacgtgaa agtataaca aaagaaaaa 
      ggttcccga agtggaagt acgtcggat aagaacctc 
      aaagtccaa actcggact tcacccccc ggaagttta 
      gctgaagaa ggccaaact aaaactggt gatgccaaa 
      agttcggct tcacgtgga agtataaca aaggacaaa 
      acaacaagt tcccgaagt agaagtacg tcggttaaa 
      agttttaaa gtccaaact cggacttca cctctaagc 
      agtttggcg gaggaacaa actgccgaa gtcaaaagt 
      ttagcttca cgtggaagt ataacaaag gaaaaaggt 
      tcccgaagt ggaagtatg tcagataaa ggccccaaa 
      gtccaaact cggacttca cttcccgag agtttgact 
      gaagaaggc caaactaaa actagtgaa gccgaaagt 
      ttggcctca cgtggaagt ataacaaaa gaacaaaca 
      acaaaatcc cgaagtgaa agtatgtcg gataaaggt 
      ctgaaagtc caaactagg acttcacct cctggcaat 
      ttgcccgaa aaagatcaa actcaaaat gcttcttct 
      actaaaagt ataacaaaa gacaagcca acaggttcc 
      cgtgcagaa aatatccca gaaaaaggc ctcaaggtt 
      caaattcgg tcttcacgc ctaagcagt ttggccgaa 
      gaaggccaa actcaaaca gccgatggt aaaactcta 
      gcctctacg aaaagtata ataagagat aagtcacca 
      agttcccga acaggaagt atctcagaa aagagccta 
      aaagtccaa attcgggct tcacctccc ggtagtttg 
      tcagaagaa gaccaaact ccaatttcc gataaacca 
      gcaactgtt ggtaaaaag aataaacga gaacggcgt 
      ccaaaagtt cgtcatcaa ccttcaacg agatcgctt 
      gtagaaccc gaaaaagat tcggcaatt tcggcttca 
      accttaatg ctcgtcagt aataaagaa cgaagacgt 
      actaaaagt cgcatgcaa gtcctttca aaattacct 
      tgaatcgcc aatatcacg tgtactgtc aagcataat 
      aaaagggta attaattct ccgttttgt aatcacagc 
      aactgttac atcacgtgt tctgtacct aattttctc 
      gctcaattc gccgaagaa aaaatccaa acgtgactt 
      attgcccca aataaatgc aaattttta ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY