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Gene information
General information
TC006711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13182931..13185789
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006711
    iBeetle screen iB_04199

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labrum potentially split - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006711 RA
      MFRFRIELA SYTKRRQSH CAALWRQIA TAIKSSVSQ 
      TDTKRLHLA ELNRLDAES AAEIALNQE RITLQEEAI 
      KTLTQHLEN LTTKRTEHI AQLKATIAE KTATFYAVR 
      QQFRKDVAK DERQLLHLT HISNKSIEF LHRIQTRGV 
      QIVNLMKTC RKFETQREK IKFLPLEPQ DLSLDEEEQ 
      EEVRRRSSG KSAPEEVKK IVISRKQSS PESQSPEPE 
      DIVGKEFAL LDCMEMFWV AYNRVDLEH REMKEERRV 
      LQGQNKHIR GMIRAVLEA VALDQSPAS KVPTRAVSK 
      TRGAKSAPL RRILV
    CDS sequence
    • >TC006711 RA
      atgttccgg ttccgcatc gaactcgcc tcttacaca 
      aagcgtcgc cagagccat tgcgccgcc ctctggaga 
      caaatagcc acagcaata aagtcctcc gtctcccaa 
      acggacaca aaacgtcta caccttgcc gaactgaac 
      cgtttagac gccgagagc gctgcagag atcgccctc 
      aaccaggag cgcataacc ctccaggag gaagcgatc 
      aaaacgctg acccagcac ttggagaac ctcaccacc 
      aaacgcacc gaacacatc gcccagctc aaagccacc 
      atcgcggaa aaaaccgcc acgttttac gccgtccgg 
      caacaattc cgcaaagat gtggccaaa gacgagcgg 
      caattgctc cacctgacc cacatctcc aacaaaagc 
      atcgaattt ttgcaccgg atccaaact cgaggcgtg 
      caaattgtg aacttaatg aaaacgtgc cggaagttt 
      gaaacccag cgcgaaaag atcaaattt ttgccactc 
      gaaccccag gacttgagt ctagacgag gaggagcaa 
      gaggaggtc aggcggagg tcgtcgggg aagagtgcc 
      ccagaagaa gtgaaaaag atcgtgatt tcgcggaaa 
      caaagttcg cccgagtct caaagtcct gagcctgag 
      gatatagta ggcaaggag tttgccttg ttggactgc 
      atggagatg ttctgggtt gcctacaac agggtggac 
      ctggagcac cgagagatg aaggaggag aggagggtt 
      ttgcaaggg cagaacaag cacatcagg gggatgatt 
      agggcggtt ttggaggcc gtggctttg gatcaatcg 
      ccggcgagt aaagtgcca acacgggcc gtttcgaag 
      acaaggggg gccaagtcg gcccccctt aggaggatc 
      ctggtctaa
    mRNA sequence
    • >TC006711 RA
      tgctcgaga aatgttccg gttccgcat cgaactcgc 
      ctcttacac aaagcgtcg ccagagcca ttgcgccgc 
      cctctggag acaaatagc cacagcaat aaagtcctc 
      cgtctccca aacggacac aaaacgtct acaccttgc 
      cgaactgaa ccgtttaga cgccgagag cgctgcaga 
      gatcgccct caaccagga gcgcataac cctccagga 
      ggaagcgat caaaacgct gacccagca cttggagaa 
      cctcaccac caaacgcac cgaacacat cgcccagct 
      caaagccac catcgcgga aaaaaccgc cacgtttta 
      cgccgtccg gcaacaatt ccgcaaaga tgtggccaa 
      agacgagcg gcaattgct ccacctgac ccacatctc 
      caacaaaag catcgaatt tttgcaccg gatccaaac 
      tcgaggcgt gcaaattgt gaacttaat gaaaacgtg 
      ccggaagtt tgaaaccca gcgcgaaaa gatcaaatt 
      tttgccact cgaacccca ggacttgag tctagacga 
      ggaggagca agaggaggt caggcggag gtcgtcggg 
      gaagagtgc cccagaaga agtgaaaaa gatcgtgat 
      ttcgcggaa acaaagttc gcccgagtc tcaaagtcc 
      tgagcctga ggatatagt aggcaagga gtttgcctt 
      gttggactg catggagat gttctgggt tgcctacaa 
      cagggtgga cctggagca ccgagagat gaaggagga 
      gaggagggt tttgcaagg gcagaacaa gcacatcag 
      ggggatgat tagggcggt tttggaggc cgtggcttt 
      ggatcaatc gccggcgag taaagtgcc aacacgggc 
      cgtttcgaa gacaagggg ggccaagtc ggcccccct 
      taggaggat cctggtcta aggtctaag gccttgaac 
      cgacgggtg ttccaataa caatcattt aaattatag 
      ttcattcat aagtaaatt agatggtta tgactaaga 
      taatgtgaa agttattgg taattacgt cttttattg 
      tttaagttg gtaatttat gctccgatt gattgtaga 
      tgagttaca gaaacaata aacagtgat aaagaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY