Status
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Gene information
General information
TC006706 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13117507..13127850
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006706
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04198

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages rounded - Penetrance (percentage of animals/eggs on slide): more than 80%
    head irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006706 RA
      MGSMAGLKR CLSQVSMMA LKASTSGEE AWLHAWHRY 
      KQSRFNARR IRKRVVFKH GDCNVVQAN VAKRRRKYL 
      QDIFTTLVD AQWRWTLLV FAFNFLGSW LVFAVVWWL 
      IAYTHGDLL EEHLNGTEP WTPCITNIY GFTSCFLFS 
      VETQHTIGY GGRTTTEEC PEAIFVMCL QSIGGVMIQ 
      AFMVGVVFA KLSRPKKRT QTLLFSRNA VISHRDGVP 
      CLMFRVGDM RKSHIIEAH VRAQLIKHK VTKEGENLP 
      FFQTELKVW VGGDGEEDK IFFIWPTMI VHKIDKNSP 
      LYNMSATDL LRERFEIVV ILEGVIEST GMTTQARSS 
      YLPSEILWG HRFQSLINF KKDSGQYEV DYALFNNTV 
      EVDTPLCSA KQLDEYRHM SSTECVGLL ADHLRTGLF 
      PSMRTHSSD TLSSMDSLS TDEHIVMKV PEHIAMKVP 
      SPIPVTVTA PEEATPLRN GSVPKATLP VPV
    CDS sequence
    • >TC006706 RA
      atggggagc atggcagga ctcaagcga tgcctctcc 
      caggtctcg atgatggcc ctcaaggcc tccacctcc 
      ggggaagag gcctggctg cacgcctgg cacagatac 
      aagcagagt cgcttcaac gcgcgtcgc atccgcaaa 
      cgcgtggtg ttcaagcat ggcgactgc aacgtggtg 
      caggccaac gtggcgaag cgtcgccgc aagtacctg 
      caggacatc ttcacgact ctggtggac gcgcaatgg 
      cgttggact ttgctcgtg ttcgcgttt aatttctta 
      ggctcgtgg ttggtcttt gccgtggtt tggtggctg 
      atagcctac acccatgga gacctcctc gaggagcat 
      cttaacgga accgagccg tggacgccg tgcatcacc 
      aacatttac ggcttcact tcgtgcttc cttttcagc 
      gtcgagacg cagcacacg atcgggtac ggcggccgg 
      accaccacc gaggagtgt ccggaggcc atcttcgtg 
      atgtgtctg cagagcatt ggcggggtg atgattcag 
      gcgttcatg gtgggggtg gtttttgcg aaattgtcg 
      aggccgaaa aaacgaact caaacgttg cttttctcg 
      cgaaatgcc gtcatttcg cacagggac ggggtgccg 
      tgtttgatg tttagggtg ggggatatg aggaaaagc 
      cacattatt gaggcgcac gtcagggca caacttatc 
      aaacataaa gttaccaaa gaaggggag aatttgccg 
      ttcttccag acggagttg aaggtatgg gtcggcggt 
      gacggcgaa gaagacaaa atcttcttc atctggccg 
      acgatgatc gtccacaaa atcgacaaa aactccccc 
      ttgtacaac atgtcggcc accgacctc ctgcgggag 
      cgcttcgag atcgtcgtc atcctggag ggggtgatc 
      gagtcgacg gggatgaca acacaggcc cgctccagc 
      tacctcccc tccgagatc ctgtggggg caccgcttc 
      cagtccttg atcaacttc aagaaggac tcggggcag 
      tacgaggtg gactacgcc cttttcaac aacactgtg 
      gaggtggac actcccctg tgcagcgcc aagcagctg 
      gacgagtac cgccacatg tccagcacc gagtgtgtt 
      ggcttgctc gcagaccac ctgaggacc gggctgttc 
      ccctccatg cgcacgcac tccagcgac accctctcc 
      agcatggac tcgctgtcc acggacgag cacatcgtg 
      atgaaggtg ccggagcac atcgccatg aaggtgccg 
      agcccgatt ccggtgaca gttaccgcc ccggaggaa 
      gccacccct ttgcgcaac gggtccgtc cccaaggcc 
      acgttacca gtcccagtc tag
    mRNA sequence
    • >TC006706 RA
      ttacaccac gaaaaaata gtcaaaaaa gccggtcag 
      tccgaccaa ccgatgata ataatcaaa atagaattt 
      gcaaactgc gccaaattt tctcgagtg tcgtcaaaa 
      tactttcag tttgttgtg gtggttcgg gtgcgacaa 
      cggtggctc tccttgcat ttacacgca caaattccg 
      ataaaaagg tccgaaaaa gtcaaagtt gatgtcaag 
      tcgcgcggt tttgctgtg tcagttgat gagttcgaa 
      ggtgatgtg cgttgttta cgctacggg ccctcggcc 
      tttgttgtt atttttgaa gctatgtgc ttgatttta 
      ttccggtca tttggtgcg agtttttgg tgaagatga 
      acgagatat tgaacggct tccactagc gaaactaat 
      cagaaacat aattttctg tggaaaaaa tttggccac 
      tagccactt cattttttt cgatgaatt atttcttat 
      ttagacgta attttggca atcttgtaa attctagaa 
      cgttctgtg tactttgaa ctgtggcct ttttgacac 
      gtgaaagtc agataagtt attatctgg gttccatat 
      tatgctttt tctgctgtc actaattta tctgcaatg 
      gtggttact gtgaaaacg ttaacactt gttaaacga 
      aaattactg tttatactg gagaacact gggagccga 
      aggtgaaat tttactttt ttgagtcat ttgcggcct 
      caggtacta acatattaa acctcggtt cataatttt 
      gttatttat aaacaaaac tttaaacgg attttaaaa 
      tttatgaac atcttttct ggaagagtt aaactagga 
      aatgaattt gcatttgaa tttcaagtc gttacatta 
      ataacacag ataactgat ggagaccgg ttttaaaat 
      attttttac tataaacta ttgccaata tgggacggc 
      aagtaataa tttttagta caaagtagt tttacacac 
      ttttatttt taactttat tataaacac ggaaatcca 
      taaaaaatc ttttgacag gtcacatca atcattaca 
      aagcagttt ttaaataga ttttttaaa ctaaataaa 
      cgaattcag aattcgaat tttacctaa tttacactc 
      aggaagaaa gtatgcacg tgtaacggt tcaaaaact 
      tcaaaacca cgcaaattt tttttccct cgcgtgctc 
      caagatttg ttaatcgca atttcagta atttcataa 
      acgatttcg tgatgaaat gtccttgtg aggcgattt 
      tttgaaata atttgctca aaataagtg gcgacaaat 
      ccggaaatc acaattaca attattctt tcaccttga 
      taaccagga tattggttt aaattctcg gcaaaaacg 
      tcacaaaga taaaactag ttcgaaaaa tcggtctaa 
      agctcagag atcgagtga ttaagtcgg cttaaaact 
      taataaata aacccgttc ggacaaatt gattcactg 
      gactggttc ttgatagat tgttcttgc aggattact 
      ttcagttca ctcaccgtt caaccgtcg caattttgc 
      ccaatttaa aatcaggtg atcgatttc acttcagcc 
      aaacaaaaa aaaaaaatt caaaacgta aacagcgca 
      catccttcg ccggattaa cgtaaatcc gagattagt 
      aatttgtgg tgacagcgt tccagttta ggacacctt 
      gtgataata tgatttcaa tatcgtcaa taaataatc 
      catctaaag gctggcctt tatctaatc atcagtggt 
      gtggtatta ataaattgg tggtgttcg tctccaata 
      aagttaatc taatgtcgg cgctaaatt aatcgactc 
      tggtgtttg gtgacgagt ttgttgtgc cattccgcc 
      accatgggg agcatggca ggactcaag cgatgcctc 
      tcccaggtc tcgatgatg gccctcaag gcctccacc 
      tccggggaa gaggcctgg ctgcacgcc tggcacaga 
      tacaagcag agtcgcttc aacgcgcgt cgcatccgc 
      aaacgcgtg gtgttcaag catggcgac tgcaacgtg 
      gtgcaggcc aacgtggcg aagcgtcgc cgcaagtac 
      ctgcaggac atcttcacg actctggtg gacgcgcaa 
      tggcgttgg actttgctc gtgttcgcg tttaatttc 
      ttaggctcg tggttggtc tttgccgtg gtttggtgg 
      ctgatagcc tacacccat ggagacctc ctcgaggag 
      catcttaac ggaaccgag ccgtggacg ccgtgcatc 
      accaacatt tacggcttc acttcgtgc ttccttttc 
      agcgtcgag acgcagcac acgatcggg tacggcggc 
      cggaccacc accgaggag tgtccggag gccatcttc 
      gtgatgtgt ctgcagagc attggcggg gtgatgatt 
      caggcgttc atggtgggg gtggttttt gcgaaattg 
      tcgaggccg aaaaaacga actcaaacg ttgcttttc 
      tcgcgaaat gccgtcatt tcgcacagg gacggggtg 
      ccgtgtttg atgtttagg gtgggggat atgaggaaa 
      agccacatt attgaggcg cacgtcagg gcacaactt 
      atcaaacat aaagttacc aaagaaggg gagaatttg 
      ccgttcttc cagacggag ttgaaggta tgggtcggc 
      ggtgacggc gaagaagac aaaatcttc ttcatctgg 
      ccgacgatg atcgtccac aaaatcgac aaaaactcc 
      cccttgtac aacatgtcg gccaccgac ctcctgcgg 
      gagcgcttc gagatcgtc gtcatcctg gagggggtg 
      atcgagtcg acggggatg acaacacag gcccgctcc 
      agctacctc ccctccgag atcctgtgg gggcaccgc 
      ttccagtcc ttgatcaac ttcaagaag gactcgggg 
      cagtacgag gtggactac gcccttttc aacaacact 
      gtggaggtg gacactccc ctgtgcagc gccaagcag 
      ctggacgag taccgccac atgtccagc accgagtgt 
      gttggcttg ctcgcagac cacctgagg accgggctg 
      ttcccctcc atgcgcacg cactccagc gacaccctc 
      tccagcatg gactcgctg tccacggac gagcacatc 
      gtgatgaag gtgccggag cacatcgcc atgaaggtg 
      ccgagcccg attccggtg acagttacc gccccggag 
      gaagccacc cctttgcgc aacgggtcc gtccccaag 
      gccacgtta ccagtccca gtctagcca gtaacttaa 
      ctcactgat acgcttatt agaatatcg ctggttcga 
      tatttgtga acaattggc attttgttg tgctgcgac 
      aacacgaaa tgccaattc ttcagttgg ttttattgt 
      ttgatctcc cgtgtcgaa atttccagc ttgtattat 
      gtataaacg atacgtaaa cggatctag gaaaattgt 
      aagtttttg ttagttgat atttgatgt agcgccacc 
      tttcattat gcttttaat tttttaact gatgtactc 
      gtagtagtt gtctttttt ttttcattt ttatacttt 
      gctcagttt gtgttcacg aggaacttt ctttttatt 
      cactcaact tttctatta tttattttt atacagagt 
      acttaatgt agttcgtaa ttttctgct tagatttta 
      cggtaggat taaggatca gttgtaaat agtctgtgc 
      catacactc ctctaagga atttttcta caaagtaaa 
      gtcaataaa tcattgcaa aacct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY