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Gene information
General information
TC006703 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13080287..13082097
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006703
    iBeetle screen iB_04197

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006703 RA
      MNDDVQSEL LDNEFRQIL LAIKPHINH IDNHYHLER 
      YRVWLERLS NAHCTERAE RNKYLLELA NQIHDNVLE 
      PPFTYNPPR GLLPRIKTF QRLQIDPAQ ISDKFFQND 
      QNQWPFIVA SQSNRNLNK NKNNTWPQK KNKPIKTKL 
      MRPFQKLGS VGQGEEKQS ETSGSWYDL AETTSETHA 
      CGDKDQEDD GNNKLGTQH LMEGLKTKE EFAKKYEQE 
      KCKGGGSFN SEKFLAEES SNRKPTAQE LEALKMRMK 
      DLLEVKNAL RDTHCLTQD RKKTLETLQ VKLSEAERE 
      KAELQVILD SQKKKFVEL DKIKSKEMN EFKEKFAHL 
      RQKEVDYLK KYHKDEVAS LEKSYSCKI RELEARIDS 
      LREEYEAKI KTLSDSNMS LLQEKEQRI HELQSELKI 
      RESQQTNQA NSEQILNLK KCLSRLDKV LHRNEKDYS 
      KKIEKLKEE LDLKEVTSQ VQLQAQKAD LIVRTSAAK 
      QEELRNALN SLESKYKKM VETVQTAAL EAKKRDEKM 
      IDELRGLLQ KHNIQIAYT V
    CDS sequence
    • >TC006703 RA
      atgaatgat gacgtacaa agtgaattg ttagataat 
      gagtttcgt caaattctc ctcgccatc aagccccac 
      atcaaccac atcgacaac cattaccac ctagaacgg 
      tacagggtg tggctggag cgcttaagc aacgcccat 
      tgcaccgaa cgggcggag cgcaacaag tacctactc 
      gagctggcc aatcagatc cacgacaac gtgctagaa 
      ccgcccttc acgtacaac ccgccccgt gggctcctc 
      ccacgaata aaaaccttc cagcgcttg caaatcgac 
      ccagcgcaa atttccgac aagtttttc caaaacgac 
      caaaaccaa tggcccttt attgtcgca tcacaatct 
      aaccgcaac ttaaataaa aataaaaac aacacctgg 
      ccgcaaaaa aagaacaaa ccgatcaaa acgaaatta 
      atgcggccg ttccaaaaa ctggggtcc gtgggacaa 
      ggggaggaa aaacagtca gaaacgagc ggctcatgg 
      tacgatttg gccgaaacc acgtccgaa acgcacgca 
      tgtggcgac aaagaccag gaggacgat gggaataac 
      aaattaggg acgcaacac ttgatggaa ggcttgaaa 
      accaaagaa gagtttgcc aaaaaatac gagcaagag 
      aaatgtaaa gggggtggt agtttcaat tctgagaaa 
      tttctagct gaggaaagc tctaaccgc aagcccacg 
      gcccaggaa ctagaagcg cttaagatg agaatgaag 
      gatttgctt gaggtcaag aacgcgctc agagacacg 
      cactgtctg acgcaagac aggaaaaag acactcgag 
      acgctccaa gtgaaatta tcggaggcg gagcgagaa 
      aaggcggag ctacaggtg atactagac tcgcagaaa 
      aaaaagttt gtggagttg gacaaaatc aagagtaaa 
      gaaatgaac gaattcaag gaaaagttt gcccacttg 
      aggcagaaa gaggtcgac tatttgaaa aagtaccac 
      aaggacgaa gtggcaagt ttggaaaag tcgtacagt 
      tgcaaaatt agggaattg gaggcgagg attgatagt 
      ttgagggag gagtacgag gcgaaaatt aaaactctg 
      agtgattca aacatgagt ttattacaa gagaaggag 
      caaagaata cacgaactt cagagcgag ttgaagatt 
      agggagagc caacaaacg aaccaggca aattctgag 
      caaatttta aacctgaaa aagtgtctc tcgagactg 
      gacaaagtc ctgcaccgg aacgaaaaa gactactcg 
      aagaaaatc gaaaagttg aaggaagag cttgacttg 
      aaggaagtg acaagtcag gtgcaactc caggcccag 
      aaggccgat ctaatcgtg cgaacctcg gccgcaaaa 
      caggaagaa ctgagaaac gcgctaaac agcctcgaa 
      agcaagtac aagaaaatg gtcgagacg gtccagacg 
      gcagctttg gaggcgaaa aaacgggac gagaaaatg 
      atcgacgag ctaaggggg ctgctgcag aagcacaac 
      atccagatt gcgtacact gtgtaa
    mRNA sequence
    • >TC006703 RA
      cgttaatta ggcgaatgc ctaagtcaa tatttttgt 
      tggtttaca cgcaaaaca agcgacatg aatgatgac 
      gtacaaagt gaattgtta gataatgag tttcgtcaa 
      attctcctc gccatcaag ccccacatc aaccacatc 
      gacaaccat taccaccta gaacggtac agggtgtgg 
      ctggagcgc ttaagcaac gcccattgc accgaacgg 
      gcggagcgc aacaagtac ctactcgag ctggccaat 
      cagatccac gacaacgtg ctagaaccg cccttcacg 
      tacaacccg ccccgtggg ctcctccca cgaataaaa 
      accttccag cgcttgcaa atcgaccca gcgcaaatt 
      tccgacaag tttttccaa aacgaccaa aaccaatgg 
      ccctttatt gtcgcatca caatctaac cgcaactta 
      aataaaaat aaaaacaac acctggccg caaaaaaag 
      aacaaaccg atcaaaacg aaattaatg cggccgttc 
      caaaaactg gggtccgtg ggacaaggg gaggaaaaa 
      cagtcagaa acgagcggc tcatggtac gatttggcc 
      gaaaccacg tccgaaacg cacgcatgt ggcgacaaa 
      gaccaggag gacgatggg aataacaaa ttagggacg 
      caacacttg atggaaggc ttgaaaacc aaagaagag 
      tttgccaaa aaatacgag caagagaaa tgtaaaggg 
      ggtggtagt ttcaattct gagaaattt ctagctgag 
      gaaagctct aaccgcaag cccacggcc caggaacta 
      gaagcgctt aagatgaga atgaaggat ttgcttgag 
      gtcaagaac gcgctcaga gacacgcac tgtctgacg 
      caagacagg aaaaagaca ctcgagacg ctccaagtg 
      aaattatcg gaggcggag cgagaaaag gcggagcta 
      caggtgata ctagactcg cagaaaaaa aagtttgtg 
      gagttggac aaaatcaag agtaaagaa atgaacgaa 
      ttcaaggaa aagtttgcc cacttgagg cagaaagag 
      gtcgactat ttgaaaaag taccacaag gacgaagtg 
      gcaagtttg gaaaagtcg tacagttgc aaaattagg 
      gaattggag gcgaggatt gatagtttg agggaggag 
      tacgaggcg aaaattaaa actctgagt gattcaaac 
      atgagttta ttacaagag aaggagcaa agaatacac 
      gaacttcag agcgagttg aagattagg gagagccaa 
      caaacgaac caggcaaat tctgagcaa attttaaac 
      ctgaaaaag tgtctctcg agactggac aaagtcctg 
      caccggaac gaaaaagac tactcgaag aaaatcgaa 
      aagttgaag gaagagctt gacttgaag gaagtgaca 
      agtcaggtg caactccag gcccagaag gccgatcta 
      atcgtgcga acctcggcc gcaaaacag gaagaactg 
      agaaacgcg ctaaacagc ctcgaaagc aagtacaag 
      aaaatggtc gagacggtc cagacggca gctttggag 
      gcgaaaaaa cgggacgag aaaatgatc gacgagcta 
      agggggctg ctgcagaag cacaacatc cagattgcg 
      tacactgtg taattgtct gaataaatt gatttcaag 
      tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY