Status
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Gene information
General information
TC006693 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12960984..12971447
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006693
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04196

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006693 RA
      MNTNDPNAL AGAHGAEAA EATTQRPPP TTSIPTDLR 
      VNTTALNAV ALSSVAKYW VLTNLLPGP IPQVSVYGL 
      PGSGRSDNQ GKITSQEAM LGQHAAGLL PADPLLLQQ 
      HSQIPVSIS TSQGVTSLS IPTGSMHLE ANHQGHQGM 
      GGHQQNDPM NQNQMNRDM SHQQQQTHT NQQNMVQVQ 
      VQDNLVSVI EDSKEQKDI IAAQLAHAQ IQLNDNQHL 
      NQQALTVQQ LQHLQVQQV LDNVVRMEN SVENTQNQA 
      NTADQTQLS DNIQIVKDE KNLQNCKLL SGTQFGLQD 
      HKGNLMDVR TADGSIVKI STNLQEQDL AKTLGVEMV 
      QNMYKVNVD DLNQLLAYH EVFGKLQGE IATTGQNIV 
      QNTNNTNVN LQQNTNNIA IVTKDEQEA STSSLPTDN 
      TATVITGNH VCDLCGKMF QFRYQLIVH RRYHTERKP 
      FTCQVCGKA FTNSQELTR HGKCHLGGS MFTCATCFH 
      VFANAASLE RHMKRHSTD KPYNCTICG KSFARKEHL 
      DNHTRCHTG ETPYRCQYC AKTFTRKEH MVNHVRKHT 
      GETPHRCEI CKKSFTRKE HFMNHVMWH TGETPHHCN 
      ICGKKYTRK EHLANHMRS HTNDTPFRC EICGKSFTR 
      KEHFTNHIM WHTGETPHR CDFCSKTFT RKEHLLNHV 
      RQHTGESPH RCGFCSKSF TRKEHLINH VRQHTGETP 
      FRCNYCPKA FTRKDHLVN HVRQHTGES PHKCTFCTK 
      SFTRKEHLN NHVRQHTGE SPHRCHFCS KSFTRKEHL 
      TNHVRIHTG ESPHRCEFC QKTFTRKEH LTNHLRQHT 
      GETQHCCNV CSKPFTRKE HLINHMRSH TGERPFACT 
      ECGKSFPLK GNLLFHQRS HNKGALADR PFRCDLCEK 
      DFMCKGHLV SHRRSHSGE RPHSCPDCG KTFVEKGNM 
      LRHLRKHTV ENGQSNDQV ATNQAPQTS NANLQIPQV 
      SQTQTAPSN NIVTQMPMH PPSNHPVVV PTANGNVLA 
      SY
    CDS sequence
    • >TC006693 RA
      atgaatacc aatgatccg aacgccctt gcgggggct 
      cacggggct gaagccgcg gaggccacc acccaacgt 
      ccaccacca accacttca atcccgacc gatcttcgc 
      gtgaatacg acagccctc aacgctgtg gcgctttcc 
      agcgtggcg aaatactgg gtgttgacg aatctcctc 
      ccgggaccc attccccaa gtgagtgtt tatgggttg 
      ccgggatcg ggacgtagt gataatcag gggaaaata 
      acatcgcag gaggctatg ttggggcaa catgcggct 
      gggttgtta ccagctgat cccttactt ttacaacaa 
      cattcgcaa attcctgtc agtatttcg acatcgcaa 
      ggggttacg agtttgtcg ataccgaca ggtagtatg 
      catctggag gccaatcac caggggcat cagggcatg 
      ggagggcat cagcagaat gatcccatg aatcagaat 
      cagatgaat cgagatatg tctcatcaa cagcaacaa 
      acccacacc aaccagcaa aacatggtg caagtccaa 
      gtccaagat aatttagtt tcagtaatt gaagatagc 
      aaagaacaa aaagatata atcgctgct caactagcc 
      catgctcag attcaatta aacgataat caacacttg 
      aatcagcag gcattgacc gttcaacaa ttacaacat 
      cttcaagtg caacaagtc cttgataat gttgtccgg 
      atggaaaat tcggtcgaa aacacgcaa aatcaagca 
      aacaccgcc gatcaaacc caactatcc gacaacatc 
      cagattgta aaagacgag aaaaactta caaaattgt 
      aagttactg tctgggaca cagtttggt ttgcaagac 
      cataaaggt aacttgatg gatgtgcgg actgctgat 
      ggtagtatt gtcaagatt tcgacgaat cttcaagag 
      caagacttg gcaaagact ttgggtgtt gaaatggtg 
      caaaatatg tacaaagtg aacgtggac gacttgaac 
      caattgttg gcgtatcat gaagttttc ggtaagctt 
      caaggtgag attgcaaca actggtcaa aacatcgtc 
      caaaataca aacaacacg aacgtgaat ctccaacaa 
      aacacaaac aacattgca atagtgaca aaagacgag 
      caggaggct tcgactagt agtttaccg accgacaac 
      accgccacg gtgattaca gggaaccac gtctgtgat 
      ttatgcggg aaaatgttt cagtttcgt taccagtta 
      atcgtccat agacgttac cacaccgaa cgtaaaccg 
      tttacgtgt caagtttgc ggtaaagcg tttaccaat 
      tcgcaggaa ctcacccgt cacgggaag tgccatcta 
      ggcggtagt atgttcacg tgtgcgacg tgtttccac 
      gtgttcgca aacgcggcc tctctggag cgtcacatg 
      aagcgacac tccaccgat aaaccgtac aattgcaca 
      atttgcggg aagtcattc gcgcggaaa gaacactta 
      gataatcac acgcggtgt catacgggg gaaaccccc 
      tacagatgt cagtattgt gccaaaacc ttcacccgc 
      aaggaacac atggtgaat cacgtgcgg aaacataca 
      ggggaaaca ccgcatcgt tgcgaaatc tgcaagaag 
      agtttcacg cggaaggaa cacttcatg aatcatgtg 
      atgtggcat acgggggaa accccccac cactgcaac 
      atatgcggg aagaagtac acacggaag gaacacttg 
      gccaatcac atgcgctcg catacgaac gatacgccg 
      ttcagatgc gagatttgt gggaaatcc ttcacgcgg 
      aaggaacac ttcacgaat cacatcatg tggcatacg 
      ggggaaacc ccccaccgg tgtgacttc tgttccaag 
      acgttcaca cgcaaggaa catctacta aatcacgtg 
      cgacaacac acgggggaa tcaccgcat cgctgtggt 
      ttctgttcc aagtcgttc acacggaag gaacatttg 
      atcaatcac gtgagacaa cataccggc gaaacgccg 
      ttcaggtgc aattactgt cccaaagct ttcacccgc 
      aaggaccat ttggttaat cacgtgcgg cagcacacg 
      ggggaatca ccccataag tgtacgttc tgtaccaag 
      tcattcacg cggaaggaa catttgaac aaccatgtg 
      agacaacat acgggggaa tccccccat aggtgtcac 
      ttttgttcc aagtccttc acccgcaag gagcatctc 
      acgaatcac gtgaggatc cacacaggg gagtcccca 
      catcggtgc gagttctgc caaaagaca ttcactcgg 
      aaggaacat ctaaccaat catttgaga caacatacg 
      ggggaaacc cagcattgt tgcaacgtt tgttccaaa 
      cccttcacc cgcaaagaa catctgatc aaccatatg 
      agatcacac accggcgaa cgcccgttt gcttgtacc 
      gaatgtgga aagtcgttt ccactcaaa ggcaacttg 
      ctcttccac caaaggtcg cacaacaag ggcgcgtta 
      gccgatcgg cccttccgt tgcgacctc tgtgagaag 
      gatttcatg tgcaaaggg catttggtg tcgcaccgt 
      cggtcacac agcggcgaa aggccacat tcgtgtccc 
      gattgtggt aaaactttc gtcgaaaaa gggaatatg 
      ttgcgacat ttgcgtaaa cataccgtc gaaaacggg 
      caaagtaat gatcaagtg gcgacgaat caagcgcct 
      caaacgtcg aatgctaac ttgcaaata ccgcaagtt 
      agtcaaacg caaacggcg cctagcaat aacatcgtg 
      acgcaaatg ccgatgcat ccgccgagt aatcatccg 
      gtcgttgtg cctaccgcc aacggaaat gtcttggct 
      agttattag
    mRNA sequence
    • >TC006693 RA
      gggtttgta atttaaaat tgtgactta aaattgggg 
      taaaggggg gcgtccgcc acactttga cctgtctca 
      gagtgcaat ttaccaaat tgtggggct gttttcgcg 
      aaacggggg cccaatgcc ggtgaataa gctttgttt 
      atgttatga cgcatcggt tcggattca ctctcggcc 
      atgttgatt ctctctgtt tattgtttg aggcgcccc 
      gcgaccgtt tctgtcttg atcgaaaat ttttcgtga 
      tttaggccg aaatgtgat tgataaggg cgataagag 
      cgatgtgcg gggtcttgc ggcgagttt gtgctaagt 
      cttgcgggt cctttgcgc gccttgagg cgtaaaatt 
      gtgaaaatc acttctcgg tttgtaatg gctacctct 
      cgcgtcccc ccgttggtg tgcagacaa tgacagtga 
      aaattttca cgtgatatt ttcgaaaat cggcttcgg 
      ttggcgatt gttgaatgg aaagcgagt tccgggggc 
      ggtgagaca ccacactga tgaatacca atgatccga 
      acgcccttg cgggggctc acggggctg aagccgcgg 
      aggccacca cccaacgtc caccaccaa ccacttcaa 
      tcccgaccg atcttcgcg tgaatacga cagccctca 
      acgctgtgg cgctttcca gcgtggcga aatactggg 
      tgttgacga atctcctcc cgggaccca ttccccaag 
      tgagtgttt atgggttgc cgggatcgg gacgtagtg 
      ataatcagg ggaaaataa catcgcagg aggctatgt 
      tggggcaac atgcggctg ggttgttac cagctgatc 
      ccttacttt tacaacaac attcgcaaa ttcctgtca 
      gtatttcga catcgcaag gggttacga gtttgtcga 
      taccgacag gtagtatgc atctggagg ccaatcacc 
      aggggcatc agggcatgg gagggcatc agcagaatg 
      atcccatga atcagaatc agatgaatc gagatatgt 
      ctcatcaac agcaacaaa cccacacca accagcaaa 
      acatggtgc aagtccaag tccaagata atttagttt 
      cagtaattg aagatagca aagaacaaa aagatataa 
      tcgctgctc aactagccc atgctcaga ttcaattaa 
      acgataatc aacacttga atcagcagg cattgaccg 
      ttcaacaat tacaacatc ttcaagtgc aacaagtcc 
      ttgataatg ttgtccgga tggaaaatt cggtcgaaa 
      acacgcaaa atcaagcaa acaccgccg atcaaaccc 
      aactatccg acaacatcc agattgtaa aagacgaga 
      aaaacttac aaaattgta agttactgt ctgggacac 
      agtttggtt tgcaagacc ataaaggta acttgatgg 
      atgtgcgga ctgctgatg gtagtattg tcaagattt 
      cgacgaatc ttcaagagc aagacttgg caaagactt 
      tgggtgttg aaatggtgc aaaatatgt acaaagtga 
      acgtggacg acttgaacc aattgttgg cgtatcatg 
      aagttttcg gtaagcttc aaggtgaga ttgcaacaa 
      ctggtcaaa acatcgtcc aaaatacaa acaacacga 
      acgtgaatc tccaacaaa acacaaaca acattgcaa 
      tagtgacaa aagacgagc aggaggctt cgactagta 
      gtttaccga ccgacaaca ccgccacgg tgattacag 
      ggaaccacg tctgtgatt tatgcggga aaatgtttc 
      agtttcgtt accagttaa tcgtccata gacgttacc 
      acaccgaac gtaaaccgt ttacgtgtc aagtttgcg 
      gtaaagcgt ttaccaatt cgcaggaac tcacccgtc 
      acgggaagt gccatctag gcggtagta tgttcacgt 
      gtgcgacgt gtttccacg tgttcgcaa acgcggcct 
      ctctggagc gtcacatga agcgacact ccaccgata 
      aaccgtaca attgcacaa tttgcggga agtcattcg 
      cgcggaaag aacacttag ataatcaca cgcggtgtc 
      atacggggg aaaccccct acagatgtc agtattgtg 
      ccaaaacct tcacccgca aggaacaca tggtgaatc 
      acgtgcgga aacatacag gggaaacac cgcatcgtt 
      gcgaaatct gcaagaaga gtttcacgc ggaaggaac 
      acttcatga atcatgtga tgtggcata cgggggaaa 
      ccccccacc actgcaaca tatgcggga agaagtaca 
      cacggaagg aacacttgg ccaatcaca tgcgctcgc 
      atacgaacg atacgccgt tcagatgcg agatttgtg 
      ggaaatcct tcacgcgga aggaacact tcacgaatc 
      acatcatgt ggcatacgg gggaaaccc cccaccggt 
      gtgacttct gttccaaga cgttcacac gcaaggaac 
      atctactaa atcacgtgc gacaacaca cgggggaat 
      caccgcatc gctgtggtt tctgttcca agtcgttca 
      cacggaagg aacatttga tcaatcacg tgagacaac 
      ataccggcg aaacgccgt tcaggtgca attactgtc 
      ccaaagctt tcacccgca aggaccatt tggttaatc 
      acgtgcggc agcacacgg gggaatcac cccataagt 
      gtacgttct gtaccaagt cattcacgc ggaaggaac 
      atttgaaca accatgtga gacaacata cgggggaat 
      ccccccata ggtgtcact tttgttcca agtccttca 
      cccgcaagg agcatctca cgaatcacg tgaggatcc 
      acacagggg agtccccac atcggtgcg agttctgcc 
      aaaagacat tcactcgga aggaacatc taaccaatc 
      atttgagac aacatacgg gggaaaccc agcattgtt 
      gcaacgttt gttccaaac ccttcaccc gcaaagaac 
      atctgatca accatatga gatcacaca ccggcgaac 
      gcccgtttg cttgtaccg aatgtggaa agtcgtttc 
      cactcaaag gcaacttgc tcttccacc aaaggtcgc 
      acaacaagg gcgcgttag ccgatcggc ccttccgtt 
      gcgacctct gtgagaagg atttcatgt gcaaagggc 
      atttggtgt cgcaccgtc ggtcacaca gcggcgaaa 
      ggccacatt cgtgtcccg attgtggta aaactttcg 
      tcgaaaaag ggaatatgt tgcgacatt tgcgtaaac 
      ataccgtcg aaaacgggc aaagtaatg atcaagtgg 
      cgacgaatc aagcgcctc aaacgtcga atgctaact 
      tgcaaatac cgcaagtta gtcaaacgc aaacggcgc 
      ctagcaata acatcgtga cgcaaatgc cgatgcatc 
      cgccgagta atcatccgg tcgttgtgc ctaccgcca 
      acggaaatg tcttggcta gttattagt gtgatattg 
      tgaggttag gtagttcct tgtattgcc atatccttg 
      aagcagtat tccctcctc gagtaggtt aagaagaga 
      gtgacaagt gaggagcac ttgacactg cgatgatat 
      tctaaagta tgccatgac gcgatggaa gcccatcat 
      ggcctacta atactcaat acgcctggg tgacttaaa 
      gattgtacc attaaagtt taattcatt catttccaa 
      ttccgtgtt gccataagt ttttgacgc aatctatta 
      tagtcaccc ggtagtata actggtggt ttctatatt 
      tttacaaac agtgaatga gcgatttat agctgattt 
      aaagctcga aagagagat tgtccaatc catattcca 
      tggttattt gatcaagaa gtaatacta atggtaatt 
      acagatgcc ctatggttg tcccctaaa gtaccgggt 
      gacttaaag attgtagca tcatattta aacgtcaaa 
      atttaatac ataattgtc attcattcc caattccgt 
      gctgccaat cgatttttg acacaatct tttatagtc 
      acccggtag tataactgg tgtttctat atttttaca 
      aacagcgaa tgagcgatt tataactga tttaaggct 
      cgaaagaga gactattca atcaatatt ccttagtta 
      tttgatcaa gaagtaata ctaatggta ataagacat 
      gtcgtatgg ttgtctatt attacctat gaatttggc 
      tcagctggt ggcgtattg agtattagg ggatcatgt 
      cccagtgag ggttccgtg cacaccctg tacatttag 
      cggtttgtg ttgtaaata attatatat attaacgag 
      gccagttga atttttgta cttatgtta agcagctta 
      gatcgtgga attttatct atactataa tattcttgt 
      gataaatga tttcgatgc gggcctatt ttgtgtatt 
      cacgaccaa tttgtaggg ttagtgcgt cacttgttt 
      tgatattaa tttttttgg tgctataat aacggggac 
      tgatgtgtt gaaaacact ttttgattt ttgcacaga 
      tctgatttt ttaccgttc aagtgacga gttttaatg 
      aagcttgct gtacataaa actatagga gaattatgc 
      taaaatgta aatttgtta ttagataat gtaacaatt 
      acttgttac tggatgttt ctgacttag ttacattgt 
      agaacaatt actatgatt acaaatgct tttttgtac 
      ataactatt atgtaaagt aacccgaaa agtgtttgt 
      agttgtaaa ttttcgaga gtaattatt ataaattta 
      tagaagcta tatattata catatacac tttagttaa 
      tatttttta gattatttt gtaatacaa ccaagatgt 
      atgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY