Status
Please wait ...
Gene information
General information
TC006676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12752423..12754681
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006676
    iBeetle screen iB_04191

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006676 RA
      MSSLRPMSS CYEHNVYEM NNESMVFQQ NDLFPLAFP 
      CMEEIRRQG RLCDVVLKI DGQDFSAHK IVLAATIPY 
      FHAMFMNDM VESRNKEIE MKCIDAMAL EALINFAYS 
      GRVILDKNN VQSIMIGAS FLQLSKVRD ACANYLLQG 
      LHPQNAIGV RDFADSLGC PSLADSAEK YIETYFHEV 
      SLHEEYLNL SLAEVKTLL NKNELRVES EEQVFEACM 
      RWIKHRDCR KEFLPELLA LVRLPLLSP LYITDRVGT 
      EEAIRYSLQ CRDLLDEAR TYHLIPERR ALMQSFKTE 
      PRACEVKGY IYVVGGLNK HGDSLSTVE YYDPKTNTW 
      HMAPPMSML RSRLGVAVL RSQLYAFGG YNGKDRLAS 
      VEVYDATKK EWSSVSPMQ CKRSALGAT ALGDIIYVC 
      GGYDGVTSL NSVERYHPL TNTWFSLAP MNKSRSAGA 
      VIACQGYIY ALGGHDGLS IFDSVERYD PNSNTWTEA 
      APMLTKRCR LGVAMLGGK LYACGGYDG STFLQTVEM 
      YNPYTNKWT YVAPMNAQR SRVALTANM GKLWAVGGY 
      DGISNLVSV EVYDPKTDQ WTYAAPMVA HEGGVGLGV 
      ISVP
    CDS sequence
    • >TC006676 RA
      atgagtagt ttgcgtccc atgagctcg tgctacgag 
      cacaatgtg tacgaaatg aacaacgag agtatggtt 
      ttccaacaa aacgacctt ttccccctg gctttcccc 
      tgcatggag gaaatccgg cgtcagggg cggctatgc 
      gacgttgtt ttgaagatt gatggccag gacttcagt 
      gcccataaa atcgtcctg gccgccacc atcccctac 
      ttccatgcc atgttcatg aacgacatg gtagagtcc 
      cggaataag gagattgaa atgaaatgc atcgacgca 
      atggcgcta gaagctctg attaacttc gcatactct 
      ggccgtgtc attctggac aaaaacaac gttcagagc 
      ataatgata ggggcctcg tttttgcaa ctgagcaaa 
      gtcagggac gcctgtgcc aattatttg ctacaaggg 
      ttgcacccc cagaacgcc ataggggtg cgtgatttt 
      gccgacagt cttgggtgc cccagcttg gccgactcg 
      gccgagaaa tacattgag acgtacttc cacgaggtg 
      tcgctgcat gaggagtac ctcaatttg agcctggct 
      gaggttaag actttgcta aacaaaaat gaattgagg 
      gtggaaagc gaggagcag gtttttgag gcttgtatg 
      aggtggatc aagcatcgg gactgtcgg aaggagttt 
      ttacccgag cttttggcc cttgtgaga ctcccgttg 
      ctgtccccg ctctacatt accgacaga gtggggacc 
      gaggaagcc attcgttac tcactgcaa tgccgtgat 
      ttgttagat gaggcacgc acttatcat ctaattccc 
      gagcgacgt gctttgatg caaagtttt aagaccgag 
      ccacgggct tgcgaagtt aaaggttac atttacgta 
      gttgggggg ctaaataaa cacggggac tcactgagc 
      actgttgag tattacgac cccaaaaca aacacctgg 
      cacatggcg ccacctatg tcaatgttg cggtctcgg 
      ttaggtgtc gcggtcctg aggagccaa ttgtacgcc 
      ttcgggggc tataatggc aaagacagg ttggccagt 
      gttgaagtt tacgacgcc actaaaaaa gaatggtcc 
      tcggtgagc cccatgcag tgtaaacga agcgcactg 
      ggggctacc gccctaggc gacataatc tacgtttgt 
      gggggctat gacggggtc acgtcgctc aattccgtg 
      gagcggtac cacccattg accaacact tggttttcg 
      ttagctccc atgaataag tcgcgcagc gctggggcc 
      gtgatcgcc tgccagggc tacatttac gcactgggg 
      gggcacgat gggctttcc atttttgat tcagtcgag 
      cgctacgat ccgaattct aatacttgg acggaggcg 
      gcccccatg ttgacgaag cggtgcagg ttgggggtg 
      gcaatgttg ggggggaag ttgtacgcg tgtgggggc 
      tacgacggg agcacgttt ttgcaaact gtggaaatg 
      tacaatcct tacactaat aagtggacg tatgtggcc 
      cccatgaat gcgcaaagg agtcgcgta gccttgacg 
      gcaaatatg ggcaaattg tgggctgtt gggggctat 
      gacgggatc tcgaatttg gtttctgtg gaggtttat 
      gacccgaag acggatcag tggacgtat gcggccccc 
      atggtggcg catgagggg ggcgtgggg ctgggggtt 
      atctctgtt ccttaa
    mRNA sequence
    • >TC006676 RA
      cgtcaaaca tttgcatta ctaaattca gaaattttt 
      gatttcttg aatgatttt gcattaaag tgggcccta 
      attaccact agtgattat gtcccagta atgtccaga 
      gtaagtgtt ccttaattg tgcttaaac tacccaaaa 
      agtgggatt agttttgtg caaaaatga gtagtttgc 
      gtcccatga gctcgtgct acgagcaca atgtgtacg 
      aaatgaaca acgagagta tggttttcc aacaaaacg 
      accttttcc ccctggctt tcccctgca tggaggaaa 
      tccggcgtc aggggcggc tatgcgacg ttgttttga 
      agattgatg gccaggact tcagtgccc ataaaatcg 
      tcctggccg ccaccatcc cctacttcc atgccatgt 
      tcatgaacg acatggtag agtcccgga ataaggaga 
      ttgaaatga aatgcatcg acgcaatgg cgctagaag 
      ctctgatta acttcgcat actctggcc gtgtcattc 
      tggacaaaa acaacgttc agagcataa tgatagggg 
      cctcgtttt tgcaactga gcaaagtca gggacgcct 
      gtgccaatt atttgctac aagggttgc acccccaga 
      acgccatag gggtgcgtg attttgccg acagtcttg 
      ggtgcccca gcttggccg actcggccg agaaataca 
      ttgagacgt acttccacg aggtgtcgc tgcatgagg 
      agtacctca atttgagcc tggctgagg ttaagactt 
      tgctaaaca aaaatgaat tgagggtgg aaagcgagg 
      agcaggttt ttgaggctt gtatgaggt ggatcaagc 
      atcgggact gtcggaagg agtttttac ccgagcttt 
      tggcccttg tgagactcc cgttgctgt ccccgctct 
      acattaccg acagagtgg ggaccgagg aagccattc 
      gttactcac tgcaatgcc gtgatttgt tagatgagg 
      cacgcactt atcatctaa ttcccgagc gacgtgctt 
      tgatgcaaa gttttaaga ccgagccac gggcttgcg 
      aagttaaag gttacattt acgtagttg gggggctaa 
      ataaacacg gggactcac tgagcactg ttgagtatt 
      acgacccca aaacaaaca cctggcaca tggcgccac 
      ctatgtcaa tgttgcggt ctcggttag gtgtcgcgg 
      tcctgagga gccaattgt acgccttcg ggggctata 
      atggcaaag acaggttgg ccagtgttg aagtttacg 
      acgccacta aaaaagaat ggtcctcgg tgagcccca 
      tgcagtgta aacgaagcg cactggggg ctaccgccc 
      taggcgaca taatctacg tttgtgggg gctatgacg 
      gggtcacgt cgctcaatt ccgtggagc ggtaccacc 
      cattgacca acacttggt tttcgttag ctcccatga 
      ataagtcgc gcagcgctg gggccgtga tcgcctgcc 
      agggctaca tttacgcac tgggggggc acgatgggc 
      tttccattt ttgattcag tcgagcgct acgatccga 
      attctaata cttggacgg aggcggccc ccatgttga 
      cgaagcggt gcaggttgg gggtggcaa tgttggggg 
      ggaagttgt acgcgtgtg ggggctacg acgggagca 
      cgtttttgc aaactgtgg aaatgtaca atccttaca 
      ctaataagt ggacgtatg tggccccca tgaatgcgc 
      aaaggagtc gcgtagcct tgacggcaa atatgggca 
      aattgtggg ctgttgggg gctatgacg ggatctcga 
      atttggttt ctgtggagg tttatgacc cgaagacgg 
      atcagtgga cgtatgcgg cccccatgg tggcgcatg 
      aggggggcg tggggctgg gggttatct ctgttcctt 
      aattgactg ttaattaag taatttatt gttttatct 
      taagaaata aaatgtttc atcaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY