Status
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Gene information
General information
TC034161
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006668,TC006670,TC006669
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12716269..12724238
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034161
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04190

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034161 RA
      MQEIPLDEI AHKDTSPAP KRPKLNFYL YLVASIANL 
      LGFSTGNAI VWVAPVLPK LLSSADNPL GRVITHSEA 
      SWVAGLLPL GAIFGPFLA GKIADKIGR KKSLLVLAL 
      IKVGSLLIT AYAHSIWLY YVSRFSIGV AIGTVFAVL 
      PMYLAEIAQ NHNRGTLAC SMGVFIAIG FNFTFLLGP 
      YLTIQNFSL VCLAPLAVF LPCFVILCP ESPVFLATK 
      HERKQLVKS LLKLRNQSI ETEIALLET SQNREPTTS 
      GLTNLLKTK SLRKAFVIS LGLISLQQS AGVSAIMSY 
      LQTIFEATG SKFAPEICA MITGTFQVF GTVLASTIV 
      DKAGRKILL LCSSAGMSV TLLLLAVYF YLQGHKFAV 
      VAKLSWLPV LSLVVFILA FSFGLGPVP WAVMAEVFP 
      ASVRSLAAS ATSVTCFVN TFVVTVAFP SMALFCGMS 
      NCFLIFAMI CLVGTVFIY KVVPETKGR SLQEIQKLL 
      EGNQLCTMS KKINWGLYF SVLSINLTS FLTGAAYSW 
      TSPVIPKLN NAEKLEENP FGRLISPFE ESWLASLIS 
      VGASIGPVL SALVVDKIG RKKTLLVLT IPMIIPHLV 
      LAFAKNITL FYLSRFFLG LGIGSVYSI VPIYVGEIA 
      EDGNRGTLG CCISVMYVS GTVFCFIVG PFLTIRTLC 
      LVLVAPAVF FLIIVSWHV PESPYYLVM VHRKEEAEL 
      ALRKLRTSY DEKELEEII KNVEASKNV KIQLGQVVK 
      SRIIRKGLC TMSKKINWG LYFSVLSIN LTSFLTGAA 
      YSWTSPVIP KLNNAEKLE ENPFGRLIS PFEESWLAS 
      LISVGASIG PVLSALVVD KIGRKKTLL VLTIPMIIP 
      HLVLAFAKN ITLYYLSRF FLGLGIGSV YSIVPIYVG 
      EIAEDGNRG TLGCCISVM YVSGTVFCF IVGPFLTIR 
      TLCLVLVAP AVFFLIIVS LHVPESPYY LVMVHRKEE 
      AEVALRKLR TSYDEKELE EIIKNVEAS KNVKIRLGQ 
      VVKSRLIRK GVLIGSGLI FFQQCSGIT VIVAYMQSI 
      FEASGSSLK PEISAIIIG LIQLTTNVV TSQLIDRLG 
      RRVLLLGSL VGMFLAHSL LGLYFWLKI NGFDSIVSQ 
      MFWVPVGSL ILYFVMFTT GVGPVSWSM LGEIFPTHV 
      RAHASTFVC CVCSVLGFV LTLFFPNLA QIIGLGFTF 
      WFFAACCGV GVAFVWKVV PETRGKSLL EIQQILEYG 
      SIKH
    CDS sequence
    • >TC034161 RA
      atgcaagaa ataccactc gacgaaatc gcccataaa 
      gacacttct ccagcccca aaaaggcca aaactcaac 
      ttctatctc tacctcgtc gcttcaatc gccaatttg 
      ctgggtttt tcaacggga aacgccatc gtttgggtg 
      gccccagtc ctcccaaaa ctcctttca agtgccgat 
      aaccccctc ggacgcgtt atcacccat tcggaggcc 
      tcatgggtg gctggcttg ctaccactg ggcgcgatc 
      ttcggccct ttcctcgcc ggaaaaatc gccgataaa 
      atcggacgt aaaaaaagt ctccttgtt ttagctctc 
      atcaaagtt ggctcgctt ctaattacg gcctacgcc 
      cactcgatt tggctctac tacgtgtcg agattttcg 
      ataggggtg gcaataggc acggttttc gcagtgctg 
      ccaatgtat ttggccgaa atcgcccaa aaccacaac 
      aggggcact ttggcgtgc tccatgggc gtttttatc 
      gcaattggg ttcaatttc accttcctc ctgggccca 
      tacctcacc atacagaat ttcagtctg gtttgtctc 
      gcccccttg gcggttttt ctcccgtgt tttgttatt 
      ttgtgtccc gaaagtccc gtttttctg gcaaccaaa 
      catgagcgc aaacaactt gttaaaagt ttgctcaaa 
      cttcgcaac caatcaatc gaaacggaa atagcgctt 
      ttggaaacg tcccaaaac cgggaacca accaccagt 
      gggcttaca aatctgctc aagacaaaa tcgttgcgt 
      aaagccttc gttatctcc ctagggctg ataagtttg 
      cagcaatcg gccggcgtg agtgcgata atgtcgtac 
      ttacagacc atcttcgag gccactggg agcaaattc 
      gcgccggag atttgtgca atgatcacc gggaccttc 
      caggttttt ggcacagtt ctggcctcc acgattgtg 
      gacaaggcc ggccgtaaa attttactc ttgtgctct 
      tcggctgga atgtccgta actttgtta cttctcgcg 
      gtgtatttc tacctccag gggcataaa tttgcagtt 
      gtggcgaag ttgtcctgg ttgcccgtt ttatcgctt 
      gtggttttt atcctggcg tttagtttt ggtctgggg 
      ccggtgcct tgggccgta atggccgag gtttttccg 
      gcaagtgtt aggtcgctg gcagcgtct gccaccagt 
      gtcacttgc tttgttaac acttttgtg gtcactgtt 
      gcgttcccg tccatggca ttgttttgc ggaatgtcg 
      aattgtttt ctgattttt gcgatgatt tgtcttgtc 
      gggacggtt tttatttat aaagtggtg cccgaaacg 
      aaagggagg agtttgcaa gagatacag aaattgtta 
      gaagggaac cagttgtgc acaatgagc aaaaaaatc 
      aactggggt ttgtacttc tccgtccta tcaattaac 
      ctgacttcg ttcctaacc ggagctgcg tactcctgg 
      acttcccct gtaatcccg aaactgaac aatgcggaa 
      aaactcgaa gaaaacccc ttcggccgt ttaatatcg 
      ccctttgag gagtcctgg cttgcgagc ttgataagc 
      gtgggggct tccatcggc cccgtttta agcgccctg 
      gttgtggac aaaatcggc cggaaaaaa accctccta 
      gtgctcact atcccgatg ataatccca catttggtc 
      ctagctttc gccaaaaac atcaccctg ttttacttg 
      agccgattt ttcctgggc cttgggata ggctctgtc 
      tattccatt gtccccatc tatgttgga gaaatcgcc 
      gaagatgga aacagaggg actttgggc tgctgcatc 
      agcgtcatg tacgtttcc gggacggtt ttttgcttc 
      atcgtgggc cctttccta accatcaga accctgtgc 
      ctggttttg gtggcccca gcggttttt ttcctcata 
      attgtcagt tggcacgtc ccggaaagt ccctattac 
      ctcgttatg gtgcaccgg aaggaagaa gctgaacta 
      gcgttgagg aaacttcga acaagttac gacgagaaa 
      gagttagaa gagataata aaaaatgtg gaagcgtca 
      aaaaacgta aaaatccaa ctcggacaa gtcgtcaaa 
      agccggata atacggaaa ggattgtgc acaatgagc 
      aaaaaaatc aactggggt ttgtacttc tccgtccta 
      tcaattaac ctgacttca ttcctaacc ggagctgcg 
      tactcctgg acttcccct gtgatcccg aaactgaac 
      aatgcagaa aaactcgaa gaaaacccc ttcggccgt 
      ttaatatcg ccctttgag gagtcctgg cttgcgagc 
      ttgataagc gtgggggct tccatcggc cccgtttta 
      agcgccctg gttgtggac aaaatcggc cggaaaaaa 
      accctccta gtgctcact atcccgatg ataatccca 
      catttggtc ctagctttc gccaaaaac atcaccctg 
      tattacttg agccgattt ttcctgggc ctcgggata 
      ggctctgtc tattccatt gtccccatc tatgttgga 
      gaaatcgcc gaagatgga aacagaggg actttgggc 
      tgctgcatc agcgtcatg tacgtttcc gggacggtt 
      ttttgcttc atcgtgggc cctttccta accatcaga 
      accctgtgc ctggttttg gtggcccca gcggttttt 
      ttcctcata attgtcagt ttgcacgtc ccggaaagt 
      ccctattac ctcgttatg gtgcaccgg aaggaagaa 
      gctgaagta gcgttaagg aaacttcgg acgagttac 
      gacgagaaa gagttagaa gagataata aaaaatgtg 
      gaagcgtca aaaaacgta aaaatccga ctcggacaa 
      gtcgtcaaa agccggcta atacggaag ggagtgttg 
      atcgggtcc ggattaatt ttcttccag caatgttcc 
      ggaatcacg gtcatcgta gcctacatg cagtcgatt 
      ttcgaagcc tccgggagc agcctcaag ccggaaatt 
      tccgcaatc ataatcggg ctcatccaa ctaaccacc 
      aacgtggtc acttcgcag ctaatcgac cgcttgggc 
      cggagagtc ctcctgttg ggttctttg gtcggaatg 
      ttcctcgca cattctctc ctggggctg tatttttgg 
      ctcaagatc aacggtttc gactcaata gtctcgcaa 
      atgttctgg gtgcctgtc ggcagtttg attctctat 
      tttgtgatg tttaccacc ggtgtgggg cctgtgagc 
      tggtcaatg cttggggag atttttcca actcatgtc 
      agagcgcac gcttccact tttgtgtgc tgtgtttgc 
      tcggttttg ggctttgtc ctaacgctg tttttccca 
      aatttggcg cagattatt gggctcggg ttcactttt 
      tggtttttt gcggcgtgt tgtggcgtt ggggttgcg 
      ttcgtgtgg aaggttgtg ccggagaca aggggcaaa 
      agtctgctt gagatacaa caaattctg gaatatggc 
      tcaataaaa cattaa
    mRNA sequence
    • >TC034161 RA
      ggccacaag cccgcttta tgcatttct tcccgcgat 
      aacaaaggt tatcattca gcgccaact aaaccaaaa 
      tgcaagaaa taccactcg acgaaatcg cccataaag 
      acacttctc cagccccaa aaaggccaa aactcaact 
      tctatctct acctcgtcg cttcaatcg ccaatttgc 
      tgggttttt caacgggaa acgccatcg tttgggtgg 
      ccccagtcc tcccaaaac tcctttcaa gtgccgata 
      accccctcg gacgcgtta tcacccatt cggaggcct 
      catgggtgg ctggcttgc taccactgg gcgcgatct 
      tcggccctt tcctcgccg gaaaaatcg ccgataaaa 
      tcggacgta aaaaaagtc tccttgttt tagctctca 
      tcaaagttg gctcgcttc taattacgg cctacgccc 
      actcgattt ggctctact acgtgtcga gattttcga 
      taggggtgg caataggca cggttttcg cagtgctgc 
      caatgtatt tggccgaaa tcgcccaaa accacaaca 
      ggggcactt tggcgtgct ccatgggcg tttttatcg 
      caattgggt tcaatttca ccttcctcc tgggcccat 
      acctcacca tacagaatt tcagtctgg tttgtctcg 
      cccccttgg cggtttttc tcccgtgtt ttgttattt 
      tgtgtcccg aaagtcccg tttttctgg caaccaaac 
      atgagcgca aacaacttg ttaaaagtt tgctcaaac 
      ttcgcaacc aatcaatcg aaacggaaa tagcgcttt 
      tggaaacgt cccaaaacc gggaaccaa ccaccagtg 
      ggcttacaa atctgctca agacaaaat cgttgcgta 
      aagccttcg ttatctccc tagggctga taagtttgc 
      agcaatcgg ccggcgtga gtgcgataa tgtcgtact 
      tacagacca tcttcgagg ccactggga gcaaattcg 
      cgccggaga tttgtgcaa tgatcaccg ggaccttcc 
      aggtttttg gcacagttc tggcctcca cgattgtgg 
      acaaggccg gccgtaaaa ttttactct tgtgctctt 
      cggctggaa tgtccgtaa ctttgttac ttctcgcgg 
      tgtatttct acctccagg ggcataaat ttgcagttg 
      tggcgaagt tgtcctggt tgcccgttt tatcgcttg 
      tggttttta tcctggcgt ttagttttg gtctggggc 
      cggtgcctt gggccgtaa tggccgagg tttttccgg 
      caagtgtta ggtcgctgg cagcgtctg ccaccagtg 
      tcacttgct ttgttaaca cttttgtgg tcactgttg 
      cgttcccgt ccatggcat tgttttgcg gaatgtcga 
      attgttttc tgatttttg cgatgattt gtcttgtcg 
      ggacggttt ttatttata aagtggtgc ccgaaacga 
      aagggagga gtttgcaag agatacaga aattgttag 
      aagggaacc agttgtgca caatgagca aaaaaatca 
      actggggtt tgtacttct ccgtcctat caattaacc 
      tgacttcgt tcctaaccg gagctgcgt actcctgga 
      cttcccctg taatcccga aactgaaca atgcggaaa 
      aactcgaag aaaacccct tcggccgtt taatatcgc 
      cctttgagg agtcctggc ttgcgagct tgataagcg 
      tgggggctt ccatcggcc ccgttttaa gcgccctgg 
      ttgtggaca aaatcggcc ggaaaaaaa ccctcctag 
      tgctcacta tcccgatga taatcccac atttggtcc 
      tagctttcg ccaaaaaca tcaccctgt tttacttga 
      gccgatttt tcctgggcc ttgggatag gctctgtct 
      attccattg tccccatct atgttggag aaatcgccg 
      aagatggaa acagaggga ctttgggct gctgcatca 
      gcgtcatgt acgtttccg ggacggttt tttgcttca 
      tcgtgggcc ctttcctaa ccatcagaa ccctgtgcc 
      tggttttgg tggccccag cggtttttt tcctcataa 
      ttgtcagtt ggcacgtcc cggaaagtc cctattacc 
      tcgttatgg tgcaccgga aggaagaag ctgaactag 
      cgttgagga aacttcgaa caagttacg acgagaaag 
      agttagaag agataataa aaaatgtgg aagcgtcaa 
      aaaacgtaa aaatccaac tcggacaag tcgtcaaaa 
      gccggataa tacggaaag gattgtgca caatgagca 
      aaaaaatca actggggtt tgtacttct ccgtcctat 
      caattaacc tgacttcat tcctaaccg gagctgcgt 
      actcctgga cttcccctg tgatcccga aactgaaca 
      atgcagaaa aactcgaag aaaacccct tcggccgtt 
      taatatcgc cctttgagg agtcctggc ttgcgagct 
      tgataagcg tgggggctt ccatcggcc ccgttttaa 
      gcgccctgg ttgtggaca aaatcggcc ggaaaaaaa 
      ccctcctag tgctcacta tcccgatga taatcccac 
      atttggtcc tagctttcg ccaaaaaca tcaccctgt 
      attacttga gccgatttt tcctgggcc tcgggatag 
      gctctgtct attccattg tccccatct atgttggag 
      aaatcgccg aagatggaa acagaggga ctttgggct 
      gctgcatca gcgtcatgt acgtttccg ggacggttt 
      tttgcttca tcgtgggcc ctttcctaa ccatcagaa 
      ccctgtgcc tggttttgg tggccccag cggtttttt 
      tcctcataa ttgtcagtt tgcacgtcc cggaaagtc 
      cctattacc tcgttatgg tgcaccgga aggaagaag 
      ctgaagtag cgttaagga aacttcgga cgagttacg 
      acgagaaag agttagaag agataataa aaaatgtgg 
      aagcgtcaa aaaacgtaa aaatccgac tcggacaag 
      tcgtcaaaa gccggctaa tacggaagg gagtgttga 
      tcgggtccg gattaattt tcttccagc aatgttccg 
      gaatcacgg tcatcgtag cctacatgc agtcgattt 
      tcgaagcct ccgggagca gcctcaagc cggaaattt 
      ccgcaatca taatcgggc tcatccaac taaccacca 
      acgtggtca cttcgcagc taatcgacc gcttgggcc 
      ggagagtcc tcctgttgg gttctttgg tcggaatgt 
      tcctcgcac attctctcc tggggctgt atttttggc 
      tcaagatca acggtttcg actcaatag tctcgcaaa 
      tgttctggg tgcctgtcg gcagtttga ttctctatt 
      ttgtgatgt ttaccaccg gtgtggggc ctgtgagct 
      ggtcaatgc ttggggaga tttttccaa ctcatgtca 
      gagcgcacg cttccactt ttgtgtgct gtgtttgct 
      cggttttgg gctttgtcc taacgctgt ttttcccaa 
      atttggcgc agattattg ggctcgggt tcacttttt 
      ggttttttg cggcgtgtt gtggcgttg gggttgcgt 
      tcgtgtgga aggttgtgc cggagacaa ggggcaaaa 
      gtctgcttg agatacaac aaattctgg aatatggct 
      caataaaac attaacgaa ttgttgttt tgttatttt 
      aaaaacttg aaattaatg ctattctgg ctgtacctt 
      aagcctaaa ataaaccta cttttaatt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY