General information | |||
TC034161
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12716269..12724238 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC034161 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04190 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC034161 RA
MQEIPLDEI AHKDTSPAP KRPKLNFYL YLVASIANL LGFSTGNAI VWVAPVLPK LLSSADNPL GRVITHSEA SWVAGLLPL GAIFGPFLA GKIADKIGR KKSLLVLAL IKVGSLLIT AYAHSIWLY YVSRFSIGV AIGTVFAVL PMYLAEIAQ NHNRGTLAC SMGVFIAIG FNFTFLLGP YLTIQNFSL VCLAPLAVF LPCFVILCP ESPVFLATK HERKQLVKS LLKLRNQSI ETEIALLET SQNREPTTS GLTNLLKTK SLRKAFVIS LGLISLQQS AGVSAIMSY LQTIFEATG SKFAPEICA MITGTFQVF GTVLASTIV DKAGRKILL LCSSAGMSV TLLLLAVYF YLQGHKFAV VAKLSWLPV LSLVVFILA FSFGLGPVP WAVMAEVFP ASVRSLAAS ATSVTCFVN TFVVTVAFP SMALFCGMS NCFLIFAMI CLVGTVFIY KVVPETKGR SLQEIQKLL EGNQLCTMS KKINWGLYF SVLSINLTS FLTGAAYSW TSPVIPKLN NAEKLEENP FGRLISPFE ESWLASLIS VGASIGPVL SALVVDKIG RKKTLLVLT IPMIIPHLV LAFAKNITL FYLSRFFLG LGIGSVYSI VPIYVGEIA EDGNRGTLG CCISVMYVS GTVFCFIVG PFLTIRTLC LVLVAPAVF FLIIVSWHV PESPYYLVM VHRKEEAEL ALRKLRTSY DEKELEEII KNVEASKNV KIQLGQVVK SRIIRKGLC TMSKKINWG LYFSVLSIN LTSFLTGAA YSWTSPVIP KLNNAEKLE ENPFGRLIS PFEESWLAS LISVGASIG PVLSALVVD KIGRKKTLL VLTIPMIIP HLVLAFAKN ITLYYLSRF FLGLGIGSV YSIVPIYVG EIAEDGNRG TLGCCISVM YVSGTVFCF IVGPFLTIR TLCLVLVAP AVFFLIIVS LHVPESPYY LVMVHRKEE AEVALRKLR TSYDEKELE EIIKNVEAS KNVKIRLGQ VVKSRLIRK GVLIGSGLI FFQQCSGIT VIVAYMQSI FEASGSSLK PEISAIIIG LIQLTTNVV TSQLIDRLG RRVLLLGSL VGMFLAHSL LGLYFWLKI NGFDSIVSQ MFWVPVGSL ILYFVMFTT GVGPVSWSM LGEIFPTHV RAHASTFVC CVCSVLGFV LTLFFPNLA QIIGLGFTF WFFAACCGV GVAFVWKVV PETRGKSLL EIQQILEYG SIKH
- >TC034161 RA
atgcaagaa ataccactc gacgaaatc gcccataaa gacacttct ccagcccca aaaaggcca aaactcaac ttctatctc tacctcgtc gcttcaatc gccaatttg ctgggtttt tcaacggga aacgccatc gtttgggtg gccccagtc ctcccaaaa ctcctttca agtgccgat aaccccctc ggacgcgtt atcacccat tcggaggcc tcatgggtg gctggcttg ctaccactg ggcgcgatc ttcggccct ttcctcgcc ggaaaaatc gccgataaa atcggacgt aaaaaaagt ctccttgtt ttagctctc atcaaagtt ggctcgctt ctaattacg gcctacgcc cactcgatt tggctctac tacgtgtcg agattttcg ataggggtg gcaataggc acggttttc gcagtgctg ccaatgtat ttggccgaa atcgcccaa aaccacaac aggggcact ttggcgtgc tccatgggc gtttttatc gcaattggg ttcaatttc accttcctc ctgggccca tacctcacc atacagaat ttcagtctg gtttgtctc gcccccttg gcggttttt ctcccgtgt tttgttatt ttgtgtccc gaaagtccc gtttttctg gcaaccaaa catgagcgc aaacaactt gttaaaagt ttgctcaaa cttcgcaac caatcaatc gaaacggaa atagcgctt ttggaaacg tcccaaaac cgggaacca accaccagt gggcttaca aatctgctc aagacaaaa tcgttgcgt aaagccttc gttatctcc ctagggctg ataagtttg cagcaatcg gccggcgtg agtgcgata atgtcgtac ttacagacc atcttcgag gccactggg agcaaattc gcgccggag atttgtgca atgatcacc gggaccttc caggttttt ggcacagtt ctggcctcc acgattgtg gacaaggcc ggccgtaaa attttactc ttgtgctct tcggctgga atgtccgta actttgtta cttctcgcg gtgtatttc tacctccag gggcataaa tttgcagtt gtggcgaag ttgtcctgg ttgcccgtt ttatcgctt gtggttttt atcctggcg tttagtttt ggtctgggg ccggtgcct tgggccgta atggccgag gtttttccg gcaagtgtt aggtcgctg gcagcgtct gccaccagt gtcacttgc tttgttaac acttttgtg gtcactgtt gcgttcccg tccatggca ttgttttgc ggaatgtcg aattgtttt ctgattttt gcgatgatt tgtcttgtc gggacggtt tttatttat aaagtggtg cccgaaacg aaagggagg agtttgcaa gagatacag aaattgtta gaagggaac cagttgtgc acaatgagc aaaaaaatc aactggggt ttgtacttc tccgtccta tcaattaac ctgacttcg ttcctaacc ggagctgcg tactcctgg acttcccct gtaatcccg aaactgaac aatgcggaa aaactcgaa gaaaacccc ttcggccgt ttaatatcg ccctttgag gagtcctgg cttgcgagc ttgataagc gtgggggct tccatcggc cccgtttta agcgccctg gttgtggac aaaatcggc cggaaaaaa accctccta gtgctcact atcccgatg ataatccca catttggtc ctagctttc gccaaaaac atcaccctg ttttacttg agccgattt ttcctgggc cttgggata ggctctgtc tattccatt gtccccatc tatgttgga gaaatcgcc gaagatgga aacagaggg actttgggc tgctgcatc agcgtcatg tacgtttcc gggacggtt ttttgcttc atcgtgggc cctttccta accatcaga accctgtgc ctggttttg gtggcccca gcggttttt ttcctcata attgtcagt tggcacgtc ccggaaagt ccctattac ctcgttatg gtgcaccgg aaggaagaa gctgaacta gcgttgagg aaacttcga acaagttac gacgagaaa gagttagaa gagataata aaaaatgtg gaagcgtca aaaaacgta aaaatccaa ctcggacaa gtcgtcaaa agccggata atacggaaa ggattgtgc acaatgagc aaaaaaatc aactggggt ttgtacttc tccgtccta tcaattaac ctgacttca ttcctaacc ggagctgcg tactcctgg acttcccct gtgatcccg aaactgaac aatgcagaa aaactcgaa gaaaacccc ttcggccgt ttaatatcg ccctttgag gagtcctgg cttgcgagc ttgataagc gtgggggct tccatcggc cccgtttta agcgccctg gttgtggac aaaatcggc cggaaaaaa accctccta gtgctcact atcccgatg ataatccca catttggtc ctagctttc gccaaaaac atcaccctg tattacttg agccgattt ttcctgggc ctcgggata ggctctgtc tattccatt gtccccatc tatgttgga gaaatcgcc gaagatgga aacagaggg actttgggc tgctgcatc agcgtcatg tacgtttcc gggacggtt ttttgcttc atcgtgggc cctttccta accatcaga accctgtgc ctggttttg gtggcccca gcggttttt ttcctcata attgtcagt ttgcacgtc ccggaaagt ccctattac ctcgttatg gtgcaccgg aaggaagaa gctgaagta gcgttaagg aaacttcgg acgagttac gacgagaaa gagttagaa gagataata aaaaatgtg gaagcgtca aaaaacgta aaaatccga ctcggacaa gtcgtcaaa agccggcta atacggaag ggagtgttg atcgggtcc ggattaatt ttcttccag caatgttcc ggaatcacg gtcatcgta gcctacatg cagtcgatt ttcgaagcc tccgggagc agcctcaag ccggaaatt tccgcaatc ataatcggg ctcatccaa ctaaccacc aacgtggtc acttcgcag ctaatcgac cgcttgggc cggagagtc ctcctgttg ggttctttg gtcggaatg ttcctcgca cattctctc ctggggctg tatttttgg ctcaagatc aacggtttc gactcaata gtctcgcaa atgttctgg gtgcctgtc ggcagtttg attctctat tttgtgatg tttaccacc ggtgtgggg cctgtgagc tggtcaatg cttggggag atttttcca actcatgtc agagcgcac gcttccact tttgtgtgc tgtgtttgc tcggttttg ggctttgtc ctaacgctg tttttccca aatttggcg cagattatt gggctcggg ttcactttt tggtttttt gcggcgtgt tgtggcgtt ggggttgcg ttcgtgtgg aaggttgtg ccggagaca aggggcaaa agtctgctt gagatacaa caaattctg gaatatggc tcaataaaa cattaa
- >TC034161 RA
ggccacaag cccgcttta tgcatttct tcccgcgat aacaaaggt tatcattca gcgccaact aaaccaaaa tgcaagaaa taccactcg acgaaatcg cccataaag acacttctc cagccccaa aaaggccaa aactcaact tctatctct acctcgtcg cttcaatcg ccaatttgc tgggttttt caacgggaa acgccatcg tttgggtgg ccccagtcc tcccaaaac tcctttcaa gtgccgata accccctcg gacgcgtta tcacccatt cggaggcct catgggtgg ctggcttgc taccactgg gcgcgatct tcggccctt tcctcgccg gaaaaatcg ccgataaaa tcggacgta aaaaaagtc tccttgttt tagctctca tcaaagttg gctcgcttc taattacgg cctacgccc actcgattt ggctctact acgtgtcga gattttcga taggggtgg caataggca cggttttcg cagtgctgc caatgtatt tggccgaaa tcgcccaaa accacaaca ggggcactt tggcgtgct ccatgggcg tttttatcg caattgggt tcaatttca ccttcctcc tgggcccat acctcacca tacagaatt tcagtctgg tttgtctcg cccccttgg cggtttttc tcccgtgtt ttgttattt tgtgtcccg aaagtcccg tttttctgg caaccaaac atgagcgca aacaacttg ttaaaagtt tgctcaaac ttcgcaacc aatcaatcg aaacggaaa tagcgcttt tggaaacgt cccaaaacc gggaaccaa ccaccagtg ggcttacaa atctgctca agacaaaat cgttgcgta aagccttcg ttatctccc tagggctga taagtttgc agcaatcgg ccggcgtga gtgcgataa tgtcgtact tacagacca tcttcgagg ccactggga gcaaattcg cgccggaga tttgtgcaa tgatcaccg ggaccttcc aggtttttg gcacagttc tggcctcca cgattgtgg acaaggccg gccgtaaaa ttttactct tgtgctctt cggctggaa tgtccgtaa ctttgttac ttctcgcgg tgtatttct acctccagg ggcataaat ttgcagttg tggcgaagt tgtcctggt tgcccgttt tatcgcttg tggttttta tcctggcgt ttagttttg gtctggggc cggtgcctt gggccgtaa tggccgagg tttttccgg caagtgtta ggtcgctgg cagcgtctg ccaccagtg tcacttgct ttgttaaca cttttgtgg tcactgttg cgttcccgt ccatggcat tgttttgcg gaatgtcga attgttttc tgatttttg cgatgattt gtcttgtcg ggacggttt ttatttata aagtggtgc ccgaaacga aagggagga gtttgcaag agatacaga aattgttag aagggaacc agttgtgca caatgagca aaaaaatca actggggtt tgtacttct ccgtcctat caattaacc tgacttcgt tcctaaccg gagctgcgt actcctgga cttcccctg taatcccga aactgaaca atgcggaaa aactcgaag aaaacccct tcggccgtt taatatcgc cctttgagg agtcctggc ttgcgagct tgataagcg tgggggctt ccatcggcc ccgttttaa gcgccctgg ttgtggaca aaatcggcc ggaaaaaaa ccctcctag tgctcacta tcccgatga taatcccac atttggtcc tagctttcg ccaaaaaca tcaccctgt tttacttga gccgatttt tcctgggcc ttgggatag gctctgtct attccattg tccccatct atgttggag aaatcgccg aagatggaa acagaggga ctttgggct gctgcatca gcgtcatgt acgtttccg ggacggttt tttgcttca tcgtgggcc ctttcctaa ccatcagaa ccctgtgcc tggttttgg tggccccag cggtttttt tcctcataa ttgtcagtt ggcacgtcc cggaaagtc cctattacc tcgttatgg tgcaccgga aggaagaag ctgaactag cgttgagga aacttcgaa caagttacg acgagaaag agttagaag agataataa aaaatgtgg aagcgtcaa aaaacgtaa aaatccaac tcggacaag tcgtcaaaa gccggataa tacggaaag gattgtgca caatgagca aaaaaatca actggggtt tgtacttct ccgtcctat caattaacc tgacttcat tcctaaccg gagctgcgt actcctgga cttcccctg tgatcccga aactgaaca atgcagaaa aactcgaag aaaacccct tcggccgtt taatatcgc cctttgagg agtcctggc ttgcgagct tgataagcg tgggggctt ccatcggcc ccgttttaa gcgccctgg ttgtggaca aaatcggcc ggaaaaaaa ccctcctag tgctcacta tcccgatga taatcccac atttggtcc tagctttcg ccaaaaaca tcaccctgt attacttga gccgatttt tcctgggcc tcgggatag gctctgtct attccattg tccccatct atgttggag aaatcgccg aagatggaa acagaggga ctttgggct gctgcatca gcgtcatgt acgtttccg ggacggttt tttgcttca tcgtgggcc ctttcctaa ccatcagaa ccctgtgcc tggttttgg tggccccag cggtttttt tcctcataa ttgtcagtt tgcacgtcc cggaaagtc cctattacc tcgttatgg tgcaccgga aggaagaag ctgaagtag cgttaagga aacttcgga cgagttacg acgagaaag agttagaag agataataa aaaatgtgg aagcgtcaa aaaacgtaa aaatccgac tcggacaag tcgtcaaaa gccggctaa tacggaagg gagtgttga tcgggtccg gattaattt tcttccagc aatgttccg gaatcacgg tcatcgtag cctacatgc agtcgattt tcgaagcct ccgggagca gcctcaagc cggaaattt ccgcaatca taatcgggc tcatccaac taaccacca acgtggtca cttcgcagc taatcgacc gcttgggcc ggagagtcc tcctgttgg gttctttgg tcggaatgt tcctcgcac attctctcc tggggctgt atttttggc tcaagatca acggtttcg actcaatag tctcgcaaa tgttctggg tgcctgtcg gcagtttga ttctctatt ttgtgatgt ttaccaccg gtgtggggc ctgtgagct ggtcaatgc ttggggaga tttttccaa ctcatgtca gagcgcacg cttccactt ttgtgtgct gtgtttgct cggttttgg gctttgtcc taacgctgt ttttcccaa atttggcgc agattattg ggctcgggt tcacttttt ggttttttg cggcgtgtt gtggcgttg gggttgcgt tcgtgtgga aggttgtgc cggagacaa ggggcaaaa gtctgcttg agatacaac aaattctgg aatatggct caataaaac attaacgaa ttgttgttt tgttatttt aaaaacttg aaattaatg ctattctgg ctgtacctt aagcctaaa ataaaccta cttttaatt t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
