Status
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Gene information
General information
TC034161
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006668,TC006670,TC006669 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12716269..12724238
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034161
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04190

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034161 RA
      MQEIPLDEI AHKDTSPAP KRPKLNFYL YLVASIANL 
LGFSTGNAI VWVAPVLPK LLSSADNPL GRVITHSEA 
SWVAGLLPL GAIFGPFLA GKIADKIGR KKSLLVLAL 
IKVGSLLIT AYAHSIWLY YVSRFSIGV AIGTVFAVL 
PMYLAEIAQ NHNRGTLAC SMGVFIAIG FNFTFLLGP 
YLTIQNFSL VCLAPLAVF LPCFVILCP ESPVFLATK 
HERKQLVKS LLKLRNQSI ETEIALLET SQNREPTTS 
GLTNLLKTK SLRKAFVIS LGLISLQQS AGVSAIMSY 
LQTIFEATG SKFAPEICA MITGTFQVF GTVLASTIV 
DKAGRKILL LCSSAGMSV TLLLLAVYF YLQGHKFAV 
VAKLSWLPV LSLVVFILA FSFGLGPVP WAVMAEVFP 
ASVRSLAAS ATSVTCFVN TFVVTVAFP SMALFCGMS 
NCFLIFAMI CLVGTVFIY KVVPETKGR SLQEIQKLL 
EGNQLCTMS KKINWGLYF SVLSINLTS FLTGAAYSW 
TSPVIPKLN NAEKLEENP FGRLISPFE ESWLASLIS 
VGASIGPVL SALVVDKIG RKKTLLVLT IPMIIPHLV 
LAFAKNITL FYLSRFFLG LGIGSVYSI VPIYVGEIA 
EDGNRGTLG CCISVMYVS GTVFCFIVG PFLTIRTLC 
LVLVAPAVF FLIIVSWHV PESPYYLVM VHRKEEAEL 
ALRKLRTSY DEKELEEII KNVEASKNV KIQLGQVVK 
SRIIRKGLC TMSKKINWG LYFSVLSIN LTSFLTGAA 
YSWTSPVIP KLNNAEKLE ENPFGRLIS PFEESWLAS 
LISVGASIG PVLSALVVD KIGRKKTLL VLTIPMIIP 
HLVLAFAKN ITLYYLSRF FLGLGIGSV YSIVPIYVG 
EIAEDGNRG TLGCCISVM YVSGTVFCF IVGPFLTIR 
TLCLVLVAP AVFFLIIVS LHVPESPYY LVMVHRKEE 
AEVALRKLR TSYDEKELE EIIKNVEAS KNVKIRLGQ 
VVKSRLIRK GVLIGSGLI FFQQCSGIT VIVAYMQSI 
FEASGSSLK PEISAIIIG LIQLTTNVV TSQLIDRLG 
RRVLLLGSL VGMFLAHSL LGLYFWLKI NGFDSIVSQ 
MFWVPVGSL ILYFVMFTT GVGPVSWSM LGEIFPTHV 
RAHASTFVC CVCSVLGFV LTLFFPNLA QIIGLGFTF 
WFFAACCGV GVAFVWKVV PETRGKSLL EIQQILEYG 
SIKH
    CDS sequence
    • >TC034161 RA
      atgcaagaa ataccactc gacgaaatc gcccataaa 
gacacttct ccagcccca aaaaggcca aaactcaac 
ttctatctc tacctcgtc gcttcaatc gccaatttg 
ctgggtttt tcaacggga aacgccatc gtttgggtg 
gccccagtc ctcccaaaa ctcctttca agtgccgat 
aaccccctc ggacgcgtt atcacccat tcggaggcc 
tcatgggtg gctggcttg ctaccactg ggcgcgatc 
ttcggccct ttcctcgcc ggaaaaatc gccgataaa 
atcggacgt aaaaaaagt ctccttgtt ttagctctc 
atcaaagtt ggctcgctt ctaattacg gcctacgcc 
cactcgatt tggctctac tacgtgtcg agattttcg 
ataggggtg gcaataggc acggttttc gcagtgctg 
ccaatgtat ttggccgaa atcgcccaa aaccacaac 
aggggcact ttggcgtgc tccatgggc gtttttatc 
gcaattggg ttcaatttc accttcctc ctgggccca 
tacctcacc atacagaat ttcagtctg gtttgtctc 
gcccccttg gcggttttt ctcccgtgt tttgttatt 
ttgtgtccc gaaagtccc gtttttctg gcaaccaaa 
catgagcgc aaacaactt gttaaaagt ttgctcaaa 
cttcgcaac caatcaatc gaaacggaa atagcgctt 
ttggaaacg tcccaaaac cgggaacca accaccagt 
gggcttaca aatctgctc aagacaaaa tcgttgcgt 
aaagccttc gttatctcc ctagggctg ataagtttg 
cagcaatcg gccggcgtg agtgcgata atgtcgtac 
ttacagacc atcttcgag gccactggg agcaaattc 
gcgccggag atttgtgca atgatcacc gggaccttc 
caggttttt ggcacagtt ctggcctcc acgattgtg 
gacaaggcc ggccgtaaa attttactc ttgtgctct 
tcggctgga atgtccgta actttgtta cttctcgcg 
gtgtatttc tacctccag gggcataaa tttgcagtt 
gtggcgaag ttgtcctgg ttgcccgtt ttatcgctt 
gtggttttt atcctggcg tttagtttt ggtctgggg 
ccggtgcct tgggccgta atggccgag gtttttccg 
gcaagtgtt aggtcgctg gcagcgtct gccaccagt 
gtcacttgc tttgttaac acttttgtg gtcactgtt 
gcgttcccg tccatggca ttgttttgc ggaatgtcg 
aattgtttt ctgattttt gcgatgatt tgtcttgtc 
gggacggtt tttatttat aaagtggtg cccgaaacg 
aaagggagg agtttgcaa gagatacag aaattgtta 
gaagggaac cagttgtgc acaatgagc aaaaaaatc 
aactggggt ttgtacttc tccgtccta tcaattaac 
ctgacttcg ttcctaacc ggagctgcg tactcctgg 
acttcccct gtaatcccg aaactgaac aatgcggaa 
aaactcgaa gaaaacccc ttcggccgt ttaatatcg 
ccctttgag gagtcctgg cttgcgagc ttgataagc 
gtgggggct tccatcggc cccgtttta agcgccctg 
gttgtggac aaaatcggc cggaaaaaa accctccta 
gtgctcact atcccgatg ataatccca catttggtc 
ctagctttc gccaaaaac atcaccctg ttttacttg 
agccgattt ttcctgggc cttgggata ggctctgtc 
tattccatt gtccccatc tatgttgga gaaatcgcc 
gaagatgga aacagaggg actttgggc tgctgcatc 
agcgtcatg tacgtttcc gggacggtt ttttgcttc 
atcgtgggc cctttccta accatcaga accctgtgc 
ctggttttg gtggcccca gcggttttt ttcctcata 
attgtcagt tggcacgtc ccggaaagt ccctattac 
ctcgttatg gtgcaccgg aaggaagaa gctgaacta 
gcgttgagg aaacttcga acaagttac gacgagaaa 
gagttagaa gagataata aaaaatgtg gaagcgtca 
aaaaacgta aaaatccaa ctcggacaa gtcgtcaaa 
agccggata atacggaaa ggattgtgc acaatgagc 
aaaaaaatc aactggggt ttgtacttc tccgtccta 
tcaattaac ctgacttca ttcctaacc ggagctgcg 
tactcctgg acttcccct gtgatcccg aaactgaac 
aatgcagaa aaactcgaa gaaaacccc ttcggccgt 
ttaatatcg ccctttgag gagtcctgg cttgcgagc 
ttgataagc gtgggggct tccatcggc cccgtttta 
agcgccctg gttgtggac aaaatcggc cggaaaaaa 
accctccta gtgctcact atcccgatg ataatccca 
catttggtc ctagctttc gccaaaaac atcaccctg 
tattacttg agccgattt ttcctgggc ctcgggata 
ggctctgtc tattccatt gtccccatc tatgttgga 
gaaatcgcc gaagatgga aacagaggg actttgggc 
tgctgcatc agcgtcatg tacgtttcc gggacggtt 
ttttgcttc atcgtgggc cctttccta accatcaga 
accctgtgc ctggttttg gtggcccca gcggttttt 
ttcctcata attgtcagt ttgcacgtc ccggaaagt 
ccctattac ctcgttatg gtgcaccgg aaggaagaa 
gctgaagta gcgttaagg aaacttcgg acgagttac 
gacgagaaa gagttagaa gagataata aaaaatgtg 
gaagcgtca aaaaacgta aaaatccga ctcggacaa 
gtcgtcaaa agccggcta atacggaag ggagtgttg 
atcgggtcc ggattaatt ttcttccag caatgttcc 
ggaatcacg gtcatcgta gcctacatg cagtcgatt 
ttcgaagcc tccgggagc agcctcaag ccggaaatt 
tccgcaatc ataatcggg ctcatccaa ctaaccacc 
aacgtggtc acttcgcag ctaatcgac cgcttgggc 
cggagagtc ctcctgttg ggttctttg gtcggaatg 
ttcctcgca cattctctc ctggggctg tatttttgg 
ctcaagatc aacggtttc gactcaata gtctcgcaa 
atgttctgg gtgcctgtc ggcagtttg attctctat 
tttgtgatg tttaccacc ggtgtgggg cctgtgagc 
tggtcaatg cttggggag atttttcca actcatgtc 
agagcgcac gcttccact tttgtgtgc tgtgtttgc 
tcggttttg ggctttgtc ctaacgctg tttttccca 
aatttggcg cagattatt gggctcggg ttcactttt 
tggtttttt gcggcgtgt tgtggcgtt ggggttgcg 
ttcgtgtgg aaggttgtg ccggagaca aggggcaaa 
agtctgctt gagatacaa caaattctg gaatatggc 
tcaataaaa cattaa
    mRNA sequence
    • >TC034161 RA
      ggccacaag cccgcttta tgcatttct tcccgcgat 
aacaaaggt tatcattca gcgccaact aaaccaaaa 
tgcaagaaa taccactcg acgaaatcg cccataaag 
acacttctc cagccccaa aaaggccaa aactcaact 
tctatctct acctcgtcg cttcaatcg ccaatttgc 
tgggttttt caacgggaa acgccatcg tttgggtgg 
ccccagtcc tcccaaaac tcctttcaa gtgccgata 
accccctcg gacgcgtta tcacccatt cggaggcct 
catgggtgg ctggcttgc taccactgg gcgcgatct 
tcggccctt tcctcgccg gaaaaatcg ccgataaaa 
tcggacgta aaaaaagtc tccttgttt tagctctca 
tcaaagttg gctcgcttc taattacgg cctacgccc 
actcgattt ggctctact acgtgtcga gattttcga 
taggggtgg caataggca cggttttcg cagtgctgc 
caatgtatt tggccgaaa tcgcccaaa accacaaca 
ggggcactt tggcgtgct ccatgggcg tttttatcg 
caattgggt tcaatttca ccttcctcc tgggcccat 
acctcacca tacagaatt tcagtctgg tttgtctcg 
cccccttgg cggtttttc tcccgtgtt ttgttattt 
tgtgtcccg aaagtcccg tttttctgg caaccaaac 
atgagcgca aacaacttg ttaaaagtt tgctcaaac 
ttcgcaacc aatcaatcg aaacggaaa tagcgcttt 
tggaaacgt cccaaaacc gggaaccaa ccaccagtg 
ggcttacaa atctgctca agacaaaat cgttgcgta 
aagccttcg ttatctccc tagggctga taagtttgc 
agcaatcgg ccggcgtga gtgcgataa tgtcgtact 
tacagacca tcttcgagg ccactggga gcaaattcg 
cgccggaga tttgtgcaa tgatcaccg ggaccttcc 
aggtttttg gcacagttc tggcctcca cgattgtgg 
acaaggccg gccgtaaaa ttttactct tgtgctctt 
cggctggaa tgtccgtaa ctttgttac ttctcgcgg 
tgtatttct acctccagg ggcataaat ttgcagttg 
tggcgaagt tgtcctggt tgcccgttt tatcgcttg 
tggttttta tcctggcgt ttagttttg gtctggggc 
cggtgcctt gggccgtaa tggccgagg tttttccgg 
caagtgtta ggtcgctgg cagcgtctg ccaccagtg 
tcacttgct ttgttaaca cttttgtgg tcactgttg 
cgttcccgt ccatggcat tgttttgcg gaatgtcga 
attgttttc tgatttttg cgatgattt gtcttgtcg 
ggacggttt ttatttata aagtggtgc ccgaaacga 
aagggagga gtttgcaag agatacaga aattgttag 
aagggaacc agttgtgca caatgagca aaaaaatca 
actggggtt tgtacttct ccgtcctat caattaacc 
tgacttcgt tcctaaccg gagctgcgt actcctgga 
cttcccctg taatcccga aactgaaca atgcggaaa 
aactcgaag aaaacccct tcggccgtt taatatcgc 
cctttgagg agtcctggc ttgcgagct tgataagcg 
tgggggctt ccatcggcc ccgttttaa gcgccctgg 
ttgtggaca aaatcggcc ggaaaaaaa ccctcctag 
tgctcacta tcccgatga taatcccac atttggtcc 
tagctttcg ccaaaaaca tcaccctgt tttacttga 
gccgatttt tcctgggcc ttgggatag gctctgtct 
attccattg tccccatct atgttggag aaatcgccg 
aagatggaa acagaggga ctttgggct gctgcatca 
gcgtcatgt acgtttccg ggacggttt tttgcttca 
tcgtgggcc ctttcctaa ccatcagaa ccctgtgcc 
tggttttgg tggccccag cggtttttt tcctcataa 
ttgtcagtt ggcacgtcc cggaaagtc cctattacc 
tcgttatgg tgcaccgga aggaagaag ctgaactag 
cgttgagga aacttcgaa caagttacg acgagaaag 
agttagaag agataataa aaaatgtgg aagcgtcaa 
aaaacgtaa aaatccaac tcggacaag tcgtcaaaa 
gccggataa tacggaaag gattgtgca caatgagca 
aaaaaatca actggggtt tgtacttct ccgtcctat 
caattaacc tgacttcat tcctaaccg gagctgcgt 
actcctgga cttcccctg tgatcccga aactgaaca 
atgcagaaa aactcgaag aaaacccct tcggccgtt 
taatatcgc cctttgagg agtcctggc ttgcgagct 
tgataagcg tgggggctt ccatcggcc ccgttttaa 
gcgccctgg ttgtggaca aaatcggcc ggaaaaaaa 
ccctcctag tgctcacta tcccgatga taatcccac 
atttggtcc tagctttcg ccaaaaaca tcaccctgt 
attacttga gccgatttt tcctgggcc tcgggatag 
gctctgtct attccattg tccccatct atgttggag 
aaatcgccg aagatggaa acagaggga ctttgggct 
gctgcatca gcgtcatgt acgtttccg ggacggttt 
tttgcttca tcgtgggcc ctttcctaa ccatcagaa 
ccctgtgcc tggttttgg tggccccag cggtttttt 
tcctcataa ttgtcagtt tgcacgtcc cggaaagtc 
cctattacc tcgttatgg tgcaccgga aggaagaag 
ctgaagtag cgttaagga aacttcgga cgagttacg 
acgagaaag agttagaag agataataa aaaatgtgg 
aagcgtcaa aaaacgtaa aaatccgac tcggacaag 
tcgtcaaaa gccggctaa tacggaagg gagtgttga 
tcgggtccg gattaattt tcttccagc aatgttccg 
gaatcacgg tcatcgtag cctacatgc agtcgattt 
tcgaagcct ccgggagca gcctcaagc cggaaattt 
ccgcaatca taatcgggc tcatccaac taaccacca 
acgtggtca cttcgcagc taatcgacc gcttgggcc 
ggagagtcc tcctgttgg gttctttgg tcggaatgt 
tcctcgcac attctctcc tggggctgt atttttggc 
tcaagatca acggtttcg actcaatag tctcgcaaa 
tgttctggg tgcctgtcg gcagtttga ttctctatt 
ttgtgatgt ttaccaccg gtgtggggc ctgtgagct 
ggtcaatgc ttggggaga tttttccaa ctcatgtca 
gagcgcacg cttccactt ttgtgtgct gtgtttgct 
cggttttgg gctttgtcc taacgctgt ttttcccaa 
atttggcgc agattattg ggctcgggt tcacttttt 
ggttttttg cggcgtgtt gtggcgttg gggttgcgt 
tcgtgtgga aggttgtgc cggagacaa ggggcaaaa 
gtctgcttg agatacaac aaattctgg aatatggct 
caataaaac attaacgaa ttgttgttt tgttatttt 
aaaaacttg aaattaatg ctattctgg ctgtacctt 
aagcctaaa ataaaccta cttttaatt t
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