General information | |||
TC006665 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12690578..12693522 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC006665 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04189 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC006665 RA
MEVGEVSEN PEDKIGQLE AEKKRIQDE FNMQRAKMK ELFLQKEDE LLRRNHENA RLNSEIEEL KRELDDLKS QLVVAGMTL ENSFEQEKR KANEEIATL QQLVHETVE ESSSSRSLY DSELKNLQT YIQQLQNEI LVLKQQKQQ SPHHSSQES SLAPSVVLN ALTKGIAKK LGADAFAAS QDSGDDPAR KVETSDHAQ EDPEVLRSL IGPLEDQIQ ALKEKLRAT DEQLQRCRE CGHSQDKND QNVSTNTSF DSPKHATSC DMCSNYEDQ LVAEQRKTK ELELKVQKA EKAAERHKE DLLKEIGFR KEIEEKWNE KKEEHKQQV AELTRSTEC AEQDLKELR QVFSQTCAE MSEMLKKLT KGREKVQEE LTNLQKEND NLVGKYTIH SQELQSEAI NLPNTVEEL HELVLKHHQ DLIIAKIGK EAAEEKVNT LQSDILLLR DQITNDQHE KEVIENNLA QEIDLLKKQ KHQLEKEKK LYLANQEKL QNTDKANLA QIAELQKQI HELQTIKRQ LEDQNSELR TRVSSLQQE LDTSETVQK DFVRLSQSL QGTYPLSSF SHPESRCNW RKSASPTPK SDGSTKKTS SSAPTVAPT SAAPNARST VDTAARSSA PRVSPELWK VGLACSRQR FVMSVILY
- >TC006665 RA
atggaagtg ggtgaagtc tccgaaaat ccggaagac aaaatcggg caattggag gccgaaaag aagcgaatc caggatgag ttcaacatg caaagggcc aaaatgaaa gaactgttc ttgcaaaaa gaagatgaa ctgttacgc agaaatcac gaaaacgca cgcttaaat tccgaaatc gaagagtta aagcgcgaa ttggacgat ttgaaaagt cagttagtt gtggcaggg atgaccctt gagaatagt tttgagcag gagaaacgt aaagccaac gaggaaatt gcaacgtta caacaacta gttcacgaa accgtggaa gagtccagc tctagtcgg agtttgtac gacagcgag cttaaaaac ttacaaacg tatatccag caattacaa aacgagatt ttagtcctg aagcagcaa aagcaacag tcgccccac cattccagc caggagtct agtctggct cctagtgtc gtccttaac gccctaact aaaggaata gctaaaaaa ttgggagct gacgcgttc gctgcctct caagacagt ggcgatgac cccgccaga aaggtagag accagcgac catgcccag gaggacccc gaggtgctg cgctcgctc attggcccc cttgaggac cagatccag gccctcaag gaaaagctg cgcgcgact gacgaacag ctacaaagg tgtcgcgag tgtggccac agccaggac aaaaacgac cagaatgtt tcgacaaac acgtcgttc gattcgccg aaacatgcc acttcgtgc gacatgtgc agcaattac gaggaccag ttggtggcc gaacagcgc aaaacgaaa gagttggaa ctgaaagta caaaaggcg gaaaaggcg gccgaaaga cacaaggag gatttgctc aaggagatc ggcttccgg aaggagatc gaggagaag tggaacgag aaaaaggag gaacataag cagcaagtg gccgaactg acccggagc acggaatgt gccgagcag gacttgaag gagttgcgg caggttttt agccagaca tgcgccgaa atgagcgaa atgttgaag aaattgacc aaagggcgg gagaaagtc caggaggaa ttaaccaat ctccaaaag gagaacgat aatttggtc gggaagtac acaattcac tcacaagag ctgcagagt gaagcgatt aatctcccg aatactgtt gaggagctt cacgagttg gtgctaaag caccaccag gatttgatt atagccaag atcgggaag gaggcggct gaggaaaaa gtcaatact ttgcagtcg gatatttta ttgttgcga gatcagatt accaacgac caacacgag aaggaagtt attgagaat aatctggca caagaaatt gatttgttg aagaagcag aaacatcag ttagagaag gagaaaaag ttgtatttg gccaatcag gagaagttg caaaacacg gataaggcc aatttggca caaattgca gagctgcag aagcaaata cacgagctg caaacaatc aaacgccag ctggaagac cagaacagc gagctccgc acgcgcgtc tcctccctg cagcaggag ctcgacacg agcgaaacg gtccagaaa gacttcgtg cgtttatcg caaagcttg cagggtacg taccccctg tccagtttt tctcaccct gagtccaga tgcaattgg agaaaatcc gcgagtccg acacccaag tccgatggc agcacgaag aagacgtcg agcagtgcc ccaactgtc gctccgact tcggcggcc ccaaacgca aggagcact gtcgacact gcggccaga tcttctgca cctcgtgtc tcaccagaa ctgtggaaa gtggggcta gcatgctcc cgtcaaagg tttgtgatg tctgtcata ctttactag
- >TC006665 RA
aacaacgac aaaaacacc acaaaattc aaaaaaaca caccgattg cgagtaatt tgtcttcac acattaatc agttattac taattaaac aattgatta acgaattag cgaattaag cgctacata atcaattga tccgctcgg tcggtaaca cgtcaattg tgacaagta tcataggtt aggagctta atttgtgat ttaattgca aaaaccaca aaattatca attcgcccg taaatatta acgaacgtg agtcagtgc tgacgtaat gattgttta cttgtgcta aaccaccaa aatggaagt gggtgaagt ctccgaaaa tccggaaga caaaatcgg gcaattgga ggccgaaaa gaagcgaat ccaggatga gttcaacat gcaaagggc caaaatgaa agaactgtt cttgcaaaa agaagatga actgttacg cagaaatca cgaaaacgc acgcttaaa ttccgaaat cgaagagtt aaagcgcga attggacga tttgaaaag tcagttagt tgtggcagg gatgaccct tgagaatag ttttgagca ggagaaacg taaagccaa cgaggaaat tgcaacgtt acaacaact agttcacga aaccgtgga agagtccag ctctagtcg gagtttgta cgacagcga gcttaaaaa cttacaaac gtatatcca gcaattaca aaacgagat tttagtcct gaagcagca aaagcaaca gtcgcccca ccattccag ccaggagtc tagtctggc tcctagtgt cgtccttaa cgccctaac taaaggaat agctaaaaa attgggagc tgacgcgtt cgctgcctc tcaagacag tggcgatga ccccgccag aaaggtaga gaccagcga ccatgccca ggaggaccc cgaggtgct gcgctcgct cattggccc ccttgagga ccagatcca ggccctcaa ggaaaagct gcgcgcgac tgacgaaca gctacaaag gtgtcgcga gtgtggcca cagccagga caaaaacga ccagaatgt ttcgacaaa cacgtcgtt cgattcgcc gaaacatgc cacttcgtg cgacatgtg cagcaatta cgaggacca gttggtggc cgaacagcg caaaacgaa agagttgga actgaaagt acaaaaggc ggaaaaggc ggccgaaag acacaagga ggatttgct caaggagat cggcttccg gaaggagat cgaggagaa gtggaacga gaaaaagga ggaacataa gcagcaagt ggccgaact gacccggag cacggaatg tgccgagca ggacttgaa ggagttgcg gcaggtttt tagccagac atgcgccga aatgagcga aatgttgaa gaaattgac caaagggcg ggagaaagt ccaggagga attaaccaa tctccaaaa ggagaacga taatttggt cgggaagta cacaattca ctcacaaga gctgcagag tgaagcgat taatctccc gaatactgt tgaggagct tcacgagtt ggtgctaaa gcaccacca ggatttgat tatagccaa gatcgggaa ggaggcggc tgaggaaaa agtcaatac tttgcagtc ggatatttt attgttgcg agatcagat taccaacga ccaacacga gaaggaagt tattgagaa taatctggc acaagaaat tgatttgtt gaagaagca gaaacatca gttagagaa ggagaaaaa gttgtattt ggccaatca ggagaagtt gcaaaacac ggataaggc caatttggc acaaattgc agagctgca gaagcaaat acacgagct gcaaacaat caaacgcca gctggaaga ccagaacag cgagctccg cacgcgcgt ctcctccct gcagcagga gctcgacac gagcgaaac ggtccagaa agacttcgt gcgtttatc gcaaagctt gcagggtac gtaccccct gtccagttt ttctcaccc tgagtccag atgcaattg gagaaaatc cgcgagtcc gacacccaa gtccgatgg cagcacgaa gaagacgtc gagcagtgc cccaactgt cgctccgac ttcggcggc cccaaacgc aaggagcac tgtcgacac tgcggccag atcttctgc acctcgtgt ctcaccaga actgtggaa agtggggct agcatgctc ccgtcaaag gtttgtgat gtctgtcat actttacta gtcaaatcg tcggcgccc tacttcagc gaagcgccc ccgatgact taagcagtt aaggttgta ttcaaatgt ctaataaat tctgttcca attt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
