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Gene information
General information
TC006643 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12293271..12321411
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006643
    iBeetle screen iB_04185

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006643 RA
      MNLINMDAE NRVVLNVGG IRHETYKAT LKKIPATRL 
SRLTEALAN YDPILNEYF FDRHPGVFA QVLNYYRTG 
KLHYPSDVC GPLFEEELE FWGLDANQV EPCCWMTYT 
QHRDTQETL AVLDRLDLD TDKPSDEEI ARKFGFEED 
YFKGTLSWW QMIKPKIWS LFDEPYSST AAKVIGVLS 
VFFICVSIL SFCLKTHPD MKVPVIKTV AVRTASNGT 
DFVIDKTDT DAHVAFFYI ECICNAWFT FEILIRFIA 
SPNKCEFIN SSVNIIDYI ATASFYIDL ILRRYAAHL 
ENADIMEFF SIIRIMRLF KLTRHSSGL KILIQTFRA 
SAKELTLLV FFLVLGIVI FASLVYYAE RIQTNPDND 
FKSIPLGLW WALVTMTTV GYGDMVPKT YIGMFVGAL 
CALAGVLTI ALPVPVIVS NFAMYYSHT QARAKLPKK 
RRRVLPVEP TRGPRLPGQ APAQPPGPV APSAQNRRM 
NAVKSNHPK DMMGGPGPK MGVMSVVTS SLNPNGLES 
NPAVMICKP KEFSSSQVN LAQGSTQCY TTLKAAKDL 
TLQE
    CDS sequence
    • >TC006643 RA
      atgaatctg atcaatatg gatgccgaa aatcgggtg 
gtcctcaac gtcggaggc atccgtcat gagacctac 
aaggccacc ttgaagaaa atcccggcg actagatta 
tcacggctg actgaagcc ttggccaac tacgatccg 
atactcaac gagtatttt ttcgaccgg catcccgga 
gttttcgcg caggttctc aattattac agaactggc 
aagctccac tacccttcg gacgtatgc ggccccttg 
ttcgaggaa gagctggaa ttctggggt ctggacgcc 
aaccaggtc gagccatgt tgttggatg acatacact 
caacatcga gacacccag gagaccctc gccgtcctt 
gatcgtctg gatttggac accgacaag cccagcgac 
gaggaaata gcacggaaa tttggcttt gaagaggat 
tatttcaaa ggcaccctc tcctggtgg cagatgatc 
aagccgaag atttggtcg cttttcgat gagccgtac 
tcatcgacg gccgcaaaa gtcataggt gtcctatca 
gtatttttc atctgcgtt tccatcctc tcgttctgt 
ttgaagact catcccgac atgaaagtg cccgtcata 
aaaaccgta gccgtccgg actgcgagc aatgggaca 
gatttcgtc atcgataaa acagacact gatgctcat 
gtcgctttt ttctacatc gagtgcatc tgcaatgca 
tggttcacg ttcgagatc ctgattcgc ttcatcgcc 
tcgcctaac aaatgcgaa ttcatcaat tcatcggtg 
aacataatc gactacatc gccaccgcg tcgttctac 
atcgatttg atcctccgt cggtacgcg gcccactta 
gaaaacgcc gacataatg gaattcttt tcaataatt 
cgaatcatg cgactgttt aaactgact cgacattcg 
tccggattg aaaattttg atccagacg ttccgcgcc 
tccgccaaa gaactgacc cttttagtg ttttttctt 
gtgttaggt atagtgatt tttgctagt ttggtctac 
tacgcggag aggatacag accaatccc gacaacgac 
ttcaaaagc attccgttg ggactgtgg tgggcactg 
gtgaccatg accacagtc gggtacggt gacatggtg 
ccaaagaca tacatcggc atgtttgta ggcgctttg 
tgcgccctt gccggtgtc ttaaccatc gcacttccg 
gttccggtc atcgtttcc aactttgcc atgtactac 
agccacacg caggcccgc gccaaattg ccgaaaaaa 
cgtcgccgc gtccttccc gtggagcca acgcgaggt 
cctcgactt ccggggcaa gcccctgcc cagcctccc 
ggtcctgtc gctccttcc gcacagaat cggcgaatg 
aacgccgta aaatcgaat catcccaaa gacatgatg 
ggtgggccc ggtcccaaa atgggtgta atgagtgta 
gtgacgtcg tcgctgaat cccaacggc ttggaatcg 
aatccagct gtgatgatc tgcaagccc aaggagttc 
tcttcgagt caagtgaac ctggcccaa ggctcgacc 
caatgctac accacactc aaagctgcc aaagacctt 
actttacag gaatga
    mRNA sequence
    • >TC006643 RA
      tcacgcaac ctgaaaagg ggtggcgtc tgcatgcgt 
caaaattac cccccgccc ccatctgcc gccgccacc 
gccaccgcc agtgtgttt cagagcggc ggcgggact 
ccgtgctcg tttcgacgc cgaaaacgc gctgaaaat 
ctcataaaa aaacacatt ttcgatagt gttcaattc 
acgtgtgtt gtggtgaaa aactgaaaa ggtgatcga 
tacaagtgt cggtttttc accgctgaa acattaatt 
aatcgcctg aaatgacgc tggaatgtc actggtgaa 
aaaatgcca aggtacgac tgacaaggc ggaaaagca 
caacacgaa aaagcgaaa gcgtcctcg agcttgaca 
caatggatt tgcggccgt aacgcgaca ctaatccgg 
taagttttc agtttcagg tgtcgaaaa atcctgaaa 
aatacatgc attttttcc acagccatc atgaatctg 
atcaatatg gatgccgaa aatcgggtg gtcctcaac 
gtcggaggc atccgtcat gagacctac aaggccacc 
ttgaagaaa atcccggcg actagatta tcacggctg 
actgaagcc ttggccaac tacgatccg atactcaac 
gagtatttt ttcgaccgg catcccgga gttttcgcg 
caggttctc aattattac agaactggc aagctccac 
tacccttcg gacgtatgc ggccccttg ttcgaggaa 
gagctggaa ttctggggt ctggacgcc aaccaggtc 
gagccatgt tgttggatg acatacact caacatcga 
gacacccag gagaccctc gccgtcctt gatcgtctg 
gatttggac accgacaag cccagcgac gaggaaata 
gcacggaaa tttggcttt gaagaggat tatttcaaa 
ggcaccctc tcctggtgg cagatgatc aagccgaag 
atttggtcg cttttcgat gagccgtac tcatcgacg 
gccgcaaaa gtcataggt gtcctatca gtatttttc 
atctgcgtt tccatcctc tcgttctgt ttgaagact 
catcccgac atgaaagtg cccgtcata aaaaccgta 
gccgtccgg actgcgagc aatgggaca gatttcgtc 
atcgataaa acagacact gatgctcat gtcgctttt 
ttctacatc gagtgcatc tgcaatgca tggttcacg 
ttcgagatc ctgattcgc ttcatcgcc tcgcctaac 
aaatgcgaa ttcatcaat tcatcggtg aacataatc 
gactacatc gccaccgcg tcgttctac atcgatttg 
atcctccgt cggtacgcg gcccactta gaaaacgcc 
gacataatg gaattcttt tcaataatt cgaatcatg 
cgactgttt aaactgact cgacattcg tccggattg 
aaaattttg atccagacg ttccgcgcc tccgccaaa 
gaactgacc cttttagtg ttttttctt gtgttaggt 
atagtgatt tttgctagt ttggtctac tacgcggag 
aggatacag accaatccc gacaacgac ttcaaaagc 
attccgttg ggactgtgg tgggcactg gtgaccatg 
accacagtc gggtacggt gacatggtg ccaaagaca 
tacatcggc atgtttgta ggcgctttg tgcgccctt 
gccggtgtc ttaaccatc gcacttccg gttccggtc 
atcgtttcc aactttgcc atgtactac agccacacg 
caggcccgc gccaaattg ccgaaaaaa cgtcgccgc 
gtccttccc gtggagcca acgcgaggt cctcgactt 
ccggggcaa gcccctgcc cagcctccc ggtcctgtc 
gctccttcc gcacagaat cggcgaatg aacgccgta 
aaatcgaat catcccaaa gacatgatg ggtgggccc 
ggtcccaaa atgggtgta atgagtgta gtgacgtcg 
tcgctgaat cccaacggc ttggaatcg aatccagct 
gtgatgatc tgcaagccc aaggagttc tcttcgagt 
caagtgaac ctggcccaa ggctcgacc caatgctac 
accacactc aaagctgcc aaagacctt actttacag 
gaatgaagt gctttcgtt tcatttgta cagtagcat 
atcgtgata cgtagtatt aagggggcg tatatcaca 
ctcggcata tctcggggg gtgggacca atcagtatg 
cacaaaaac taagtgtta ggagaaacc aattctaga 
tgtaactcc cggctattg tccaccatt ctaaatttc 
tcgatttct cctcattgt tattgttgt aaattaagg 
gtccatgtt gaatttaca aagtttatg gcgggttta 
tatatcaca tatctttgc gtttcgaat tcggaccaa 
gaaattttt taacgatca ttgtgatac acgaggttt 
tttcggtta ggaggggtt tcaatcgct gtattactg 
attcagatt ttggccttt gctctttac tgtaatata 
atgtagatc tagggttaa ataagcgac tgtattact 
aaataacta aattgagac actcaactc tcaactaaa 
cgatagaaa tactgctcg tattacact cacaggcaa 
gtgtaataa ctgcagtat tgcattgta ttatagtaa 
taataaatt taaataaat gacaaaaca aatatttcc 
atgttacga gcgactttg ttatatata cacattaaa 
cattgtaat aagtacact tatttcacc ttctcacca 
cgtaattat acgataagt tgttggatt cttcctgta 
catgatata aattgtaga aatttcgat aaataaatc 
atacaaaat taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY