Status
Please wait ...
Gene information
General information
TC006642 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12275801..12290092
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006642
    iBeetle screen iB_04184

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006642 RA
      MDGENRIIL NVGGIRYET YKATLKKIP ATRLSRLTE 
      ALANYDPIL NEYFFDRHP GVFAQILNY YRTGKLHYP 
      TNVCGPLFE EELEFWGLD SNQVEPCCW STYSVHRDT 
      QATLAILDK LDIDTERPS DEEVARMFG YEEAYFNGT 
      LTTWQKFKP KIWALFDEP YSSSAAKIV AGASVFFIC 
      ASVLSFCLK THHGFHVDC DKIRRNVTD DANCTDPHP 
      YFLTVEHIC NAWFTFEFV VRLIVAPNV VEFVKSPVN 
      IIDFAATLS FYVDMILIF EKKSQLLDF FSIIRIFRL 
      FKLTRHSPG LKILIHTFK ASATELGLL VFFLVLGIV 
      VFASLIFYA EKLEENPKN NFKSIPEGL WWAIVTMTT 
      VGYGDMVPK TYAGMFVGA LCALAGVLT IALPVPVIV 
      SNFSMFYSH TQARSKLPK KRRRVLPVE QPRRKKVET 
      ATNRRMNAI KHHHPAVFK DAFGGAKLG NVNGVNVIG 
      LALQGPSVP SIAVLHHNG PYNLQGGEP VPSLPLQPR 
      PATTGSVDM LPLQPKYLP NFDRESRSE PREIPPRPD 
      ASPLKSTEA EIGLASVPA MAIGSSTEV LFSIVENQA 
      GFRRNSFLA PKSPDCEVV KSPSTEMTT SMESNGSDR 
      K
    CDS sequence
    • >TC006642 RA
      atggatggg gaaaatcgc atcattctc aacgttggc 
      ggcatccgc tacgagacg tacaaagca accttgaaa 
      aaaatccca gctactaga ttatcacgc ctgactgaa 
      gctctggcc aactacgac cccatcctc aacgagtat 
      ttcttcgac cggcatccg ggcgtgttc gcacaaata 
      ctcaactat tacagaact ggtaagttg cattatcca 
      accaacgtg tgcggccct ttgttcgaa gaggagctg 
      gagttctgg gggttggac tcgaaccag gtcgagccc 
      tgctgctgg agcacgtac agcgtccat agggacacc 
      caggccact ctcgccatt ttggataag ctcgacata 
      gacacagag cggccgagc gatgaggaa gtggctcga 
      atgtttggc tatgaggag gcttatttt aacgggact 
      ttgacgacg tggcagaag tttaagccg aaaatttgg 
      gcgcttttt gatgagccg tactcgtcg tcggcggct 
      aagatcgtc gccggagcg tcagttttc ttcatctgt 
      gcttcggtc ctctccttc tgcctcaaa acccaccac 
      ggcttccac gtcgactgc gacaagatc cgcagaaac 
      gtcaccgac gacgccaac tgcaccgac ccgcacccg 
      tacttcctc acagtggag cacatctgc aacgcctgg 
      ttcaccttc gagttcgtc gtcaggctc attgtggct 
      cctaacgtg gtcgaattc gtaaaatcc cccgtcaac 
      atcatcgac ttcgccgcc actttgagt ttctacgtc 
      gacatgatc ctcatcttc gagaagaaa tcgcaactt 
      ttggacttt ttcagcatc atccgaatt ttccgtctc 
      ttcaagctc actcgtcac tcccccggc ctgaaaatc 
      ctcatccat acgttcaag gcgtcggcc accgagctg 
      ggattactg gtgtttttt ctagttttg ggaattgtg 
      gtgttcgcg agtttgatt ttctacgcc gagaagctg 
      gaggagaat cccaaaaac aatttcaag agcatcccg 
      gagggatta tggtgggct atcgtgacc atgaccacg 
      gtgggctat ggcgacatg gtcccgaaa acctacgct 
      gggatgttc gttggggct ttgtgtgcc cttgctggg 
      gttctgact attgcactc ccagtcccg gtcatcgtt 
      tccaacttt tccatgttt tattcacac acccaggct 
      aggtctaaa ctcccgaaa aaacggaga agagtcctt 
      ccagtcgaa caaccgcgg cgaaaaaaa gtcgagact 
      gcgacaaat cgacgaatg aatgcaatc aaacatcat 
      catcctgcc gtttttaaa gatgcgttc ggtggcgcc 
      aaattagga aacgtgaac ggagtcaac gtcatcggc 
      ttggccctt caaggccct tccgtccct agcatagca 
      gtcctacat cacaatgga ccttacaat ttacaaggc 
      ggcgagcct gttccatcc cttccacta caaccgcgc 
      cccgccacg acgggtagc gtcgacatg cttcccctc 
      caacccaaa tacctcccg aatttcgat cgggaatcg 
      cgatccgaa ccgcgcgaa atcccccca agacccgat 
      gcgagtcct ttaaagtcc accgaggcc gaaatcggc 
      ctcgccagc gtcccggca atggcaatc gggagttcc 
      accgaagtt cttttcagc attgtcgag aatcaggct 
      ggttttcgg cgcaattcg ttcctcgca ccgaaaagt 
      cccgattgt gaagtggta aaatcaccg agtactgaa 
      atgacgacg tcaatggag tcgaacgga agtgaccgg 
      aagtaa
    mRNA sequence
    • >TC006642 RA
      aggcgtgcg attaactgc tagtttttc caccacaaa 
      atgttatgg atggggaaa atcgcatca ttctcaacg 
      ttggcggca tccgctacg agacgtaca aagcaacct 
      tgaaaaaaa tcccagcta ctagattat cacgcctga 
      ctgaagctc tggccaact acgacccca tcctcaacg 
      agtatttct tcgaccggc atccgggcg tgttcgcac 
      aaatactca actattaca gaactggta agttgcatt 
      atccaacca acgtgtgcg gccctttgt tcgaagagg 
      agctggagt tctgggggt tggactcga accaggtcg 
      agccctgct gctggagca cgtacagcg tccataggg 
      acacccagg ccactctcg ccattttgg ataagctcg 
      acatagaca cagagcggc cgagcgatg aggaagtgg 
      ctcgaatgt ttggctatg aggaggctt attttaacg 
      ggactttga cgacgtggc agaagttta agccgaaaa 
      tttgggcgc tttttgatg agccgtact cgtcgtcgg 
      cggctaaga tcgtcgccg gagcgtcag ttttcttca 
      tctgtgctt cggtcctct ccttctgcc tcaaaaccc 
      accacggct tccacgtcg actgcgaca agatccgca 
      gaaacgtca ccgacgacg ccaactgca ccgacccgc 
      acccgtact tcctcacag tggagcaca tctgcaacg 
      cctggttca ccttcgagt tcgtcgtca ggctcattg 
      tggctccta acgtggtcg aattcgtaa aatcccccg 
      tcaacatca tcgacttcg ccgccactt tgagtttct 
      acgtcgaca tgatcctca tcttcgaga agaaatcgc 
      aacttttgg actttttca gcatcatcc gaattttcc 
      gtctcttca agctcactc gtcactccc ccggcctga 
      aaatcctca tccatacgt tcaaggcgt cggccaccg 
      agctgggat tactggtgt tttttctag ttttgggaa 
      ttgtggtgt tcgcgagtt tgattttct acgccgaga 
      agctggagg agaatccca aaaacaatt tcaagagca 
      tcccggagg gattatggt gggctatcg tgaccatga 
      ccacggtgg gctatggcg acatggtcc cgaaaacct 
      acgctggga tgttcgttg gggctttgt gtgcccttg 
      ctggggttc tgactattg cactcccag tcccggtca 
      tcgtttcca acttttcca tgttttatt cacacaccc 
      aggctaggt ctaaactcc cgaaaaaac ggagaagag 
      tccttccag tcgaacaac cgcggcgaa aaaaagtcg 
      agactgcga caaatcgac gaatgaatg caatcaaac 
      atcatcatc ctgccgttt ttaaagatg cgttcggtg 
      gcgccaaat taggaaacg tgaacggag tcaacgtca 
      tcggcttgg cccttcaag gcccttccg tccctagca 
      tagcagtcc tacatcaca atggacctt acaatttac 
      aaggcggcg agcctgttc catcccttc cactacaac 
      cgcgccccg ccacgacgg gtagcgtcg acatgcttc 
      ccctccaac ccaaatacc tcccgaatt tcgatcggg 
      aatcgcgat ccgaaccgc gcgaaatcc ccccaagac 
      ccgatgcga gtcctttaa agtccaccg aggccgaaa 
      tcggcctcg ccagcgtcc cggcaatgg caatcggga 
      gttccaccg aagttcttt tcagcattg tcgagaatc 
      aggctggtt ttcggcgca attcgttcc tcgcaccga 
      aaagtcccg attgtgaag tggtaaaat caccgagta 
      ctgaaatga cgacgtcaa tggagtcga acggaagtg 
      accggaagt aatttttgt cttttgtcg atttttcaa 
      gcacgattt cctgcaaac actcggccc ttgcaacct 
      tcgatgttt cgatgtgtt ttgtgtttc aggaggaga 
      ggcgtgcgg agggaatgc tcattgtaa ttaggagag 
      tatttttat aataacctt tttcattgt tggattaat 
      aaatgcttt atcagtct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY