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Gene information
General information
TC006641 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12264471..12268665
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006641
    iBeetle screen iB_04183

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006641 RA
      MFQVKLSHV CTIEHVASR SFLPPLPPD ASCCARFLR 
      FLKKMILIT EINPNSMNF FRSTAEILD ERRQQVKSG 
      SWIIHPFSI FRLYYEIYM AIVLFLWLI YLPLDASYD 
      NYLTKEIFI INYCFNALG IVDIFVNFT TGYKSKKKE 
      TIVMKWSSI ACNYMFSFY FLGDVLALT PELPAYANN 
      RKLDSLLDL LSLTKTVRI FTYLNYLET LFNYFRIKA 
      GAQTVVKMF TLAFLLIHD LACIFPMMA TSSEIFDTS 
      GKIEMKLNL HISEENLAT DFKSLYIRN LYTACSYIL 
      GVDIRFKFH YPWSFYVTA ILGYFIGKF LLLFATEKL 
      KKEINYHTC RRLVNNVQI FQSLTRELV EEILHHLKP 
      EIYLPNDII VKAGSEGDC MYFLASGTV AVVSPSGKE 
      ICHLEDGDY FGEICLLYS NLKRTANII ALEISEVYK 
      LDRKSFKKS MENNLPLYK KLQADAKKR YRETRDIER 
      MIMTASHRQ IFDTQSIAS NNQSANLFN VN
    CDS sequence
    • >TC006641 RA
      atgtttcag gtgaaactg tcccacgtt tgcacaatc 
      gagcatgtg gcgtctcgc agtttcttg ccccctttg 
      cccccggac gcgtcctgt tgcgcccgt ttcttgcgt 
      ttcctgaaa aagatgatc ctcatcact gaaatcaac 
      ccaaacagc atgaacttt ttcaggagc acggctgaa 
      atcctcgac gagcgccga caacaggtc aagtcaggg 
      tcgtggatc attcaccct ttcagtatt ttcagactc 
      tactacgag atttacatg gcgattgtg ctgttcctc 
      tggctgatt tatctgcct ttggacgcg tcctatgac 
      aattatctc acgaaagaa atcttcatt attaattat 
      tgtttcaat gcgcttgga attgtcgat atttttgtg 
      aattttaca acggggtat aagagcaag aagaaggag 
      acgattgta atgaagtgg tcgtcaatc gcttgtaac 
      tacatgttt agtttttat tttctgggg gacgtttta 
      gccctcact cctgaatta cctgcttat gcgaacaac 
      cgcaagctt gacagttta ctcgacttg ttatcatta 
      accaaaact gtgcgaatt ttcacctac ttgaattat 
      ttggagacg cttttcaac tatttcaga atcaaagct 
      ggggcccaa actgtggtg aaaatgttc actttggcg 
      tttcttctc attcatgat ctcgcttgt atttttcca 
      atgatggca acttcgagt gaaattttt gacacgtct 
      ggtaaaata gagatgaaa cttaatctg cacataagt 
      gaggaaaat ctggccacg gatttcaaa tccttgtac 
      ataagaaac ctctacacg gcctgttcc tatattcta 
      ggggttgat attcgattc aaatttcat tatccttgg 
      tcgttttat gttacggcc attcttgga tattttatc 
      gggaagttt ttgctcctt tttgccaca gaaaaactc 
      aaaaaagag atcaattat cacacatgc cgtcgcttg 
      gtcaacaac gtccaaatt ttccaatct ctaacacgc 
      gaactcgtc gaagaaatc ctccaccac ctcaaaccc 
      gaaatctat ctcccgaac gacatcatc gtcaaggcg 
      ggctctgag ggcgactgc atgtatttc ctggcttct 
      ggaactgta gcagtggtc tcaccctca ggcaaagaa 
      atttgtcac ctcgaggat ggcgattat ttcggcgag 
      atttgtctg ctttactcg aatttgaaa cggacggct 
      aacataatc gcgcttgaa attagcgaa gtgtacaaa 
      ttggaccga aaatcgttc aaaaaatca atggaaaat 
      aatttacct ctctacaag aaactgcaa gctgatgct 
      aagaagaga tacagggag acaagggac atagaaagg 
      atgattatg acggcttct catcggcag attttcgac 
      acgcaatcg atcgcatcg aacaatcag tcagccaat 
      ctttttaac gttaattaa
    mRNA sequence
    • >TC006641 RA
      ttctaaggc aaacttttg acagtatta ttttttatt 
      tttttgcat aaaaataaa aatacgttt tttggtcaa 
      gtgggcgct tttcagtct gaaaagtcg cgccatgtt 
      tcaggtgaa actgtccca cgtttgcac aatcgagca 
      tgtggcgtc tcgcagttt cttgccccc tttgccccc 
      ggacgcgtc ctgttgcgc ccgtttctt gcgtttcct 
      gaaaaagat gatcctcat cactgaaat caacccaaa 
      cagcatgaa ctttttcag gagcacggc tgaaatcct 
      cgacgagcg ccgacaaca ggtcaagtc agggtcgtg 
      gatcattca ccctttcag tattttcag actctacta 
      cgagattta catggcgat tgtgctgtt cctctggct 
      gatttatct gcctttgga cgcgtccta tgacaatta 
      tctcacgaa agaaatctt cattattaa ttattgttt 
      caatgcgct tggaattgt cgatatttt tgtgaattt 
      tacaacggg gtataagag caagaagaa ggagacgat 
      tgtaatgaa gtggtcgtc aatcgcttg taactacat 
      gtttagttt ttattttct gggggacgt tttagccct 
      cactcctga attacctgc ttatgcgaa caaccgcaa 
      gcttgacag tttactcga cttgttatc attaaccaa 
      aactgtgcg aattttcac ctacttgaa ttatttgga 
      gacgctttt caactattt cagaatcaa agctggggc 
      ccaaactgt ggtgaaaat gttcacttt ggcgtttct 
      tctcattca tgatctcgc ttgtatttt tccaatgat 
      ggcaacttc gagtgaaat ttttgacac gtctggtaa 
      aatagagat gaaacttaa tctgcacat aagtgagga 
      aaatctggc cacggattt caaatcctt gtacataag 
      aaacctcta cacggcctg ttcctatat tctaggggt 
      tgatattcg attcaaatt tcattatcc ttggtcgtt 
      ttatgttac ggccattct tggatattt tatcgggaa 
      gtttttgct cctttttgc cacagaaaa actcaaaaa 
      agagatcaa ttatcacac atgccgtcg cttggtcaa 
      caacgtcca aattttcca atctctaac acgcgaact 
      cgtcgaaga aatcctcca ccacctcaa acccgaaat 
      ctatctccc gaacgacat catcgtcaa ggcgggctc 
      tgagggcga ctgcatgta tttcctggc ttctggaac 
      tgtagcagt ggtctcacc ctcaggcaa agaaatttg 
      tcacctcga ggatggcga ttatttcgg cgagatttg 
      tctgcttta ctcgaattt gaaacggac ggctaacat 
      aatcgcgct tgaaattag cgaagtgta caaattgga 
      ccgaaaatc gttcaaaaa atcaatgga aaataattt 
      acctctcta caagaaact gcaagctga tgctaagaa 
      gagatacag ggagacaag ggacataga aaggatgat 
      tatgacggc ttctcatcg gcagatttt cgacacgca 
      atcgatcgc atcgaacaa tcagtcagc caatctttt 
      taacgttaa ttaatttat tccattaaa taaacttaa 
      gccaataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY