Status
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Gene information
General information
TC006634 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12164301..12167385
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006634
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04182

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006634 RA
      MSISTRTSD LSSSAASRN SDLLENKHS KYFLFPQRY 
      IFCLLASLI ILNAASIQF TLEVIALEN FSSGKRLRT 
      NDTCTITPQ IRRRPRRRP KNESLLNYD WDLKTRYLI 
      KDSFYWGTL VTSIPGGVL SDEYGGKYI VSLSVLFSA 
      FFNLVAPAI IDLGHSVGL IIMIRVGLG FCRGVLDAG 
      LMALIARWA PVYERATIT SVIFGSRLL GEQFSYVMH 
      KAFENFTWR PMFYFFASV HLVLGFFWH LLVYPSPKQ 
      NPFMTLNEK RYLEEDPDL SLVDGWKKI PWRRILKSK 
      PVWGLCCLH FATTWAWTV YRKTLHLYL PRVLKSEVV 
      WESAWWTPF FLYYILAIF YGFVADWFV NGQHASVTA 
      MRKVYALLG NFGPALCLI FVTFERCNH FTVMIFTTM 
      ALCLDAMHF ASNMVNALD LTSNFVGIV TSITGAIEF 
      LIFALSTQL INNLFTTED SLSQWNFLL WFTCGVQVG 
      GTTLFLLLG DAERQAWNY VIKGVN
    CDS sequence
    • >TC006634 RA
      atgtctatt tcgacccgc acttcagac ctcagttca 
      tccgcagcc tctcgcaac tctgacctc ctcgaaaac 
      aaacactca aaatatttc ctcttcccc caacggtac 
      atcttttgc ctccttgcc tccctcata atcctcaac 
      gcagcgtcc atccaattc acgttggaa gtcatagct 
      ttggagaac ttctcctcg ggaaaacgc ctccgaacc 
      aacgacact tgcaccatc acaccccag atacgaaga 
      aggcccaga aggcggccc aaaaacgaa tcgcttttg 
      aactacgac tgggatctc aaaacgcgc tatttaatc 
      aaagattcg ttctattgg ggcacttta gtaacctcc 
      atacctggg ggagtgctt tcggatgaa tacggcggc 
      aaatacatt gttagtttg agtgtttta ttttcggcg 
      tttttcaat ttggtggct cctgcgatc atcgatttg 
      ggccacagt gtggggtta attatcatg attcgagta 
      ggcctgggt ttttgtcgg ggggttttg gacgcgggg 
      ttgatggcg cttatcgca cgttgggct ccagtttac 
      gaacgtgcc accataacg agtgttatc ttcgggagt 
      agactttta ggcgagcag ttctcgtat gttatgcat 
      aaagctttc gaaaatttc acttggagg ccgatgttc 
      tatttcttt gcgagtgtt catctagtt ttgggattt 
      ttttggcac ctgctggtg tacccaagt cccaaacaa 
      aacccgttc atgacactg aatgagaag aggtatttg 
      gaggaagac ccagatttg agtttggtc gatggttgg 
      aagaaaatc ccctggagg aggattctc aagtcgaaa 
      cctgtgtgg gggttgtgt tgtttgcac tttgcaacg 
      acttgggcc tggactgtg tacaggaaa acacttcat 
      ttgtatttg cccagggtt ttgaagtcg gaagttgtg 
      tgggagtcg gcttggtgg acaccgttt tttctctat 
      tatatcttg gcgattttt tatgggttt gtggccgat 
      tggtttgtt aatgggcaa catgcgagt gtgactgct 
      atgcgaaaa gtttacgcc cttttaggg aactttggc 
      ccggctttg tgtctcatc tttgtgacg tttgaacgt 
      tgtaaccac ttcacagtt atgattttt accacgatg 
      gccttatgt ttggacgca atgcacttt gcctcaaat 
      atggtcaat gcgttagat ttgacatcg aatttcgtt 
      ggaatcgtg acctcaatc acgggagca atcgaattt 
      ttgattttt gcattaagt acccaactg atcaataat 
      ttatttacg actgaagat tctttgagc caatggaac 
      ttcttactt tggttcacg tgtggcgtc caagtcggt 
      ggtaccacc ttgtttcta ctacttggg gacgcagag 
      agacaagct tggaattat gtcataaag ggagtcaat 
      taa
    mRNA sequence
    • >TC006634 RA
      tgtcaaaaa tttcaaaat tttccgctt caatgtcta 
      tttcgaccc gcacttcag acctcagtt catccgcag 
      cctctcgca actctgacc tcctcgaaa acaaacact 
      caaaatatt tcctcttcc cccaacggt acatctttt 
      gcctccttg cctccctca taatcctca acgcagcgt 
      ccatccaat tcacgttgg aagtcatag ctttggaga 
      acttctcct cgggaaaac gcctccgaa ccaacgaca 
      cttgcacca tcacacccc agatacgaa gaaggccca 
      gaaggcggc ccaaaaacg aatcgcttt tgaactacg 
      actgggatc tcaaaacgc gctatttaa tcaaagatt 
      cgttctatt ggggcactt tagtaacct ccatacctg 
      ggggagtgc tttcggatg aatacggcg gcaaataca 
      ttgttagtt tgagtgttt tattttcgg cgtttttca 
      atttggtgg ctcctgcga tcatcgatt tgggccaca 
      gtgtggggt taattatca tgattcgag taggcctgg 
      gtttttgtc ggggggttt tggacgcgg ggttgatgg 
      cgcttatcg cacgttggg ctccagttt acgaacgtg 
      ccaccataa cgagtgtta tcttcggga gtagacttt 
      taggcgagc agttctcgt atgttatgc ataaagctt 
      tcgaaaatt tcacttgga ggccgatgt tctatttct 
      ttgcgagtg ttcatctag ttttgggat ttttttggc 
      acctgctgg tgtacccaa gtcccaaac aaaacccgt 
      tcatgacac tgaatgaga agaggtatt tggaggaag 
      acccagatt tgagtttgg tcgatggtt ggaagaaaa 
      tcccctgga ggaggattc tcaagtcga aacctgtgt 
      gggggttgt gttgtttgc actttgcaa cgacttggg 
      cctggactg tgtacagga aaacacttc atttgtatt 
      tgcccaggg ttttgaagt cggaagttg tgtgggagt 
      cggcttggt ggacaccgt tttttctct attatatct 
      tggcgattt tttatgggt ttgtggccg attggtttg 
      ttaatgggc aacatgcga gtgtgactg ctatgcgaa 
      aagtttacg cccttttag ggaactttg gcccggctt 
      tgtgtctca tctttgtga cgtttgaac gttgtaacc 
      acttcacag ttatgattt ttaccacga tggccttat 
      gtttggacg caatgcact ttgcctcaa atatggtca 
      atgcgttag atttgacat cgaatttcg ttggaatcg 
      tgacctcaa tcacgggag caatcgaat ttttgattt 
      ttgcattaa gtacccaac tgatcaata atttattta 
      cgactgaag attctttga gccaatgga acttcttac 
      tttggttca cgtgtggcg tccaagtcg gtggtacca 
      ccttgtttc tactacttg gggacgcag agagacaag 
      cttggaatt atgtcataa agggagtca attaaaaca 
      atttttcga aaaaatctc tttagttga ttgctattt 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY