Status
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Gene information
General information
TC006608 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11938921..11951584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006608
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04180

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006608 RA
      MISFHKPIG HKTTKNRQP EIEEKRRPP SRLPRHSRL 
      QNPRQIPLQ DFPIHLVGP ARAHLEEPQ RPQRARLEN 
      PPPWHFLPP LNQTLVRLF VQKPLFAHS QEGVVSVGR 
      VDQQVPDEA KNDVHPSRQ VEHRPPGVV HEGLVHGDG 
      DLLPQRRAP VDQTRHQGA FARRRPTGQ QGQNRRLQN 
      PLEAPVQHP ERDHKPHLA RTQVDDRGR HQVAERREK 
      HRKGDDVLA PQLVRDETA GDNSQQAAP VERVFDNEP 
      GLETPVVDQ QRAVSRPLG DLFPGVGGA RARVAGVGG 
      FVRVEVFHC DDLEGGVDR VTTANNILL LHLGAIDVL 
      LGVLFLAFS VPGFSKSVW LSDGLPCIL HGFLYNLLH 
      PLALWTICG LNCDRFYAI AAPLHYSHI VNAKKVLVG 
      LAIGWTISL LLCLPPFFK VAPYTYIPG LEACAPDFA 
      YGSGTLWYS TLYTAFTLL LPATLIICC NLKILMIAR 
      YHRHRIASA IYEVTLSAQ VTITHQRNP FFVPTVTAP 
      NAGPPKFKG RSAIYSVLQ LVGSLALLY LPYYSLIIW 
      EATSGSFEK ISKRIHPHF FTTAMALLT CSPPVNGFL 
      YGVKSKPLR KTFKNYWRK KQTKSEVNQ EIQARTPST 
      CGSRRPSLT PLGFLTKPT LQSRLSEAL LDVQKVVNS 
      PQKHKIKRI TTELSWRPA SANSLNFLA RDDDKRIKQ 
      TSSCNTLQV PISDTEISV ITEDEKPVQ KTPKPTNLF 
      LQKFFGTSD NDKKSEVVQ NILDGTPKR SPRITITRT 
      CSEESDTPR RESMTRKHS LSSTTLLER KWRQLRECE 
      ERNVPTTKP LLDNSSNGS DSSESETSS GKIFMTLDA 
      KLDDQILLT WKDRPRPVL LKQKTVQSH DVVLH
    CDS sequence
    • >TC006608 RA
      atgatttcc tttcacaaa ccaatcggc cacaaaacc 
      acaaaaaat cgtcagcca gaaatagag gaaaaacgg 
      cgtccacca agccgactc ccacggcac tcccgactt 
      caaaaccct cggcaaata ccactccag gattttcct 
      atacacctg gtaggacca gctcgagcc cacttggag 
      gaaccccag cgaccacaa cgcgcccga cttgagaat 
      cctccccca tggcacttt cttccaccc ctcaaccag 
      actctcgtc cgcctcttc gtccaaaaa ccgcttttc 
      gcgcacagt caagaaggg gttgtctct gtgggacgg 
      gtagaccag caagtgcca gacgaagca aaaaacgac 
      gtgcacccc agccgacaa gtagaacat cggcctcca 
      ggtgtagtt catgaaggc cttgtgcat ggcgatggc 
      gatctgctc ccccaaaga cgcgctccc gtagaccag 
      actcgccat cagggcgcg ttcgctcgg cggcgccca 
      acgggccag cagggacag aaccgccga ctgcaaaac 
      cccttggaa gcacccgtt cagcacccg gagcgcgac 
      cacaaaccc caccttgcc cgcacccag gtcgacgac 
      cgcgggcgc catcaggtg gcagaacgc cgagaaaag 
      cacagaaag ggtgacgac gtacttgcc ccccaactg 
      gtccgagac gaaaccgcc ggggataat agtcaacag 
      gcggctcca gtagaacga gtctttgat aatgagccg 
      ggtctcgag actccagtc gtagaccag cagcgagcg 
      gtttcgcgg ccgcttggc gatttgttt cctggtgtc 
      gggggtgct agggcacgt gtcgctggt gttggtggt 
      tttgtccga gtcgaagta ttccactgc gatgacctc 
      gagggcgga gtggatcga gtgacgact gccaataac 
      attctcctg ttgcaccta ggagccatc gacgtcctt 
      ttaggagtt ttattcctg gcgttctca gtgccggga 
      ttttctaaa tcggtatgg ttgtcagac ggccttccg 
      tgcattctt cacggcttt ttgtacaat ctcttgcac 
      cccttagcg ctatggact atctgcgga ctcaattgt 
      gacagattt tacgccatc gctgccccg ctgcactac 
      agccacatc gtcaatgcc aaaaaggtc ctcgtgggc 
      ttggccatc ggctggacc atctcgctc ctcctctgc 
      ctccccccc ttttttaaa gtggcccct tacacctac 
      atccccggc ctggaggcg tgcgccccc gacttcgcc 
      tacggctcc ggcacccta tggtactcc accctctac 
      accgccttc actctcctc ctccccgcc accctcatc 
      atctgttgc aacctcaag atcttgatg atcgcgcgc 
      taccaccgc caccgcatc gcctccgcc atctacgaa 
      gtgacgctc tcagcccaa gtgacaata acacaccaa 
      agaaaccca ttttttgta ccaacagtc accgccccc 
      aacgccggc cccccaaag ttcaagggc cgcagcgcc 
      atctactct gtactccaa ctggtgggg tccctagcc 
      ctcctgtat cttccctac tacagtttg atcatctgg 
      gaggcgacc agcggctcc tttgagaag atctccaag 
      cggattcat ccgcatttc ttcacaact gccatggct 
      ttgctcacg tgctcgccc ccagtcaac ggtttcttg 
      tacggagtg aagagcaaa ccgctgcgg aagaccttc 
      aagaactac tggcgcaaa aaacagacc aagagcgaa 
      gtcaaccaa gagattcaa gcacggacg ccgtcgact 
      tgcggctcc aggaggccg tcactcaca cccctgggg 
      ttcctaacc aaaccaacg ttacaatcc cgcctctct 
      gaagcgcta ctggacgtg caaaaggtg gtaaacagc 
      ccacaaaag cacaaaatt aaacgaatc acgaccgag 
      ctgtcctgg cggccggcc tccgccaac agtctcaat 
      ttcctggca cgggacgac gataaaaga atcaaacag 
      acgtccagt tgcaacact ttgcaagtc ccaatcagc 
      gacaccgaa atctccgtt atcacagaa gacgagaaa 
      cctgtgcaa aaaaccccc aaacccacc aatctattt 
      ttgcaaaag ttcttcggc acttcggac aatgacaag 
      aagagtgag gtggtgcag aacatcctt gacggcacc 
      ccgaagcgc tcccccagg atcacaatc accaggacc 
      tgctccgag gagagcgac accccccgc agggagagc 
      atgaccagg aagcactcc ctgtccagc accacgctc 
      ctggagcgc aagtggcgc cagctgcgg gagtgtgaa 
      gagcgaaac gtccccaca acgaaaccg ctgctggac 
      aattcgagc aacgggagc gactcctca gagtccgaa 
      acctccagc gggaagatt ttcatgacg ttggacgcg 
      aaactagac gaccaaatt ttgctcacg tggaaagac 
      aggccgagg cccgttctg ctgaagcag aaaacagta 
      caatcacac gacgttgtt ttgcattag
    mRNA sequence
    • >TC006608 RA
      gaacagttg tcacactta gatgatttc ctttcacaa 
      accaatcgg ccacaaaac cacaaaaaa tcgtcagcc 
      agaaataga ggaaaaacg gcgtccacc aagccgact 
      cccacggca ctcccgact tcaaaaccc tcggcaaat 
      accactcca ggattttcc tatacacct ggtaggacc 
      agctcgagc ccacttgga ggaacccca gcgaccaca 
      acgcgcccg acttgagaa tcctccccc atggcactt 
      tcttccacc cctcaacca gactctcgt ccgcctctt 
      cgtccaaaa accgctttt cgcgcacag tcaagaagg 
      ggttgtctc tgtgggacg ggtagacca gcaagtgcc 
      agacgaagc aaaaaacga cgtgcaccc cagccgaca 
      agtagaaca tcggcctcc aggtgtagt tcatgaagg 
      ccttgtgca tggcgatgg cgatctgct cccccaaag 
      acgcgctcc cgtagacca gactcgcca tcagggcgc 
      gttcgctcg gcggcgccc aacgggcca gcagggaca 
      gaaccgccg actgcaaaa ccccttgga agcacccgt 
      tcagcaccc ggagcgcga ccacaaacc ccaccttgc 
      ccgcaccca ggtcgacga ccgcgggcg ccatcaggt 
      ggcagaacg ccgagaaaa gcacagaaa gggtgacga 
      cgtacttgc cccccaact ggtccgaga cgaaaccgc 
      cggggataa tagtcaaca ggcggctcc agtagaacg 
      agtctttga taatgagcc gggtctcga gactccagt 
      cgtagacca gcagcgagc ggtttcgcg gccgcttgg 
      cgatttgtt tcctggtgt cgggggtgc tagggcacg 
      tgtcgctgg tgttggtgg ttttgtccg agtcgaagt 
      attccactg cgatgacct cgagggcgg agtggatcg 
      agtgacgac tgccaataa cattctcct gttgcacct 
      aggagccat cgacgtcct tttaggagt tttattcct 
      ggcgttctc agtgccggg attttctaa atcggtatg 
      gttgtcaga cggccttcc gtgcattct tcacggctt 
      tttgtacaa tctcttgca ccccttagc gctatggac 
      tatctgcgg actcaattg tgacagatt ttacgccat 
      cgctgcccc gctgcacta cagccacat cgtcaatgc 
      caaaaaggt cctcgtggg cttggccat cggctggac 
      catctcgct cctcctctg cctcccccc cttttttaa 
      agtggcccc ttacaccta catccccgg cctggaggc 
      gtgcgcccc cgacttcgc ctacggctc cggcaccct 
      atggtactc caccctcta caccgcctt cactctcct 
      cctccccgc caccctcat catctgttg caacctcaa 
      gatcttgat gatcgcgcg ctaccaccg ccaccgcat 
      cgcctccgc catctacga agtgacgct ctcagccca 
      agtgacaat aacacacca aagaaaccc attttttgt 
      accaacagt caccgcccc caacgccgg ccccccaaa 
      gttcaaggg ccgcagcgc catctactc tgtactcca 
      actggtggg gtccctagc cctcctgta tcttcccta 
      ctacagttt gatcatctg ggaggcgac cagcggctc 
      ctttgagaa gatctccaa gcggattca tccgcattt 
      cttcacaac tgccatggc tttgctcac gtgctcgcc 
      cccagtcaa cggtttctt gtacggagt gaagagcaa 
      accgctgcg gaagacctt caagaacta ctggcgcaa 
      aaaacagac caagagcga agtcaacca agagattca 
      agcacggac gccgtcgac ttgcggctc caggaggcc 
      gtcactcac acccctggg gttcctaac caaaccaac 
      gttacaatc ccgcctctc tgaagcgct actggacgt 
      gcaaaaggt ggtaaacag cccacaaaa gcacaaaat 
      taaacgaat cacgaccga gctgtcctg gcggccggc 
      ctccgccaa cagtctcaa tttcctggc acgggacga 
      cgataaaag aatcaaaca gacgtccag ttgcaacac 
      tttgcaagt cccaatcag cgacaccga aatctccgt 
      tatcacaga agacgagaa acctgtgca aaaaacccc 
      caaacccac caatctatt tttgcaaaa gttcttcgg 
      cacttcgga caatgacaa gaagagtga ggtggtgca 
      gaacatcct tgacggcac cccgaagcg ctcccccag 
      gatcacaat caccaggac ctgctccga ggagagcga 
      caccccccg cagggagag catgaccag gaagcactc 
      cctgtccag caccacgct cctggagcg caagtggcg 
      ccagctgcg ggagtgtga agagcgaaa cgtccccac 
      aacgaaacc gctgctgga caattcgag caacgggag 
      cgactcctc agagtccga aacctccag cgggaagat 
      tttcatgac gttggacgc gaaactaga cgaccaaat 
      tttgctcac gtggaaaga caggccgag gcccgttct 
      gctgaagca gaaaacagt acaatcaca cgacgttgt 
      tttgcatta gtcttagtt caatgtgat gtctgaatg 
      tgataatag agtagttga tggtgcgga gcgattatt 
      ttgctactg ttggccgct tgttagttc acacacctg 
      ttaaaatga cattattca agttttaaa tgtctaaac 
      aatgttaac attccattt ttgagtatt gagcaatgt 
      aagaatgtg tgcacggtc tgcttcgtt cacttcggt 
      tcaaactgc gctaaatca cagggtata aatacaatc 
      tcttcgtag gggtaaaga gtcaatgca tatcttaca 
      taataataa ccgatcagt attaaagcg actcattga 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY