Status
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Gene information
General information
TC006591 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11735631..11740062
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006591
    iBeetle screen iB_04178

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006591 RA
      MMDSELSNT DICRVCRSE GLPDRPLFH PCICTGSIK 
      WIHQECLMQ WMRYSRKEY CELCSYRFS FTPIYSPDM 
      PRRLPVRDL AAGLLSSIG TAIKYWLHY TLVAIAWLG 
      IVPLTACRI YRALFAGTV DAILTLPFE LLSMENLAS 
      DIFQGCFVV TCTLFAFAG LVWLREQIL HGGGPDWLQ 
      RDNVGVAED APPPVLNNN IPEDVEENN GQEPEVAPQ 
      GENQGEQQE ENEVNGGED NNWIPMEWD RAAEEITWE 
      RLLGLDGSL MFLEHVFWV ISLNTLFIL IFAYSPYHM 
      GLMTLSLVN MREAASASH FEGLLTTLV GYCTVGLFF 
      VFFHRVAGV LGFYKAKRV LGLCYVVVK VSMLSVVEI 
      GILPLVCGW WLDICSLAM FDASLKDRE SSFSAAPGT 
      SIFIHWLIG MVYVYYFAS FVLLLREIL RPGVLWFLR 
      NFNDPDFSP IQEMIHLPI LEHIRKLLI SAVIFGSVV 
      LLMLWLPIR ILKILLPSF LPYTVSLNS ETQVNELSL 
      ELLLLQVIL PALLEQSHT RIWLKGLVR NWCRAVAWI 
      LNIHSYLLG NSDGQSPNE AGQAVANPP EPGAGLGAA 
      HHALLMMEP PSGFQPYER PSFFVARLV GLLILVCVS 
      LVTFSLTAL TLPVWLGRR VMALWLVGA PPPGPQIST 
      NSAQTEVKV HELYTAACG LYLCWLSAR AVTLVMSWL 
      PQGRAAMIS RLKQWCVLG LKTMIASTI LLGVIPLLF 
      GMLLELVVI VPLRVPIHQ TPILFIWQD WALGVLYTK 
      IACAVTMMG PDWFLRAAI ERAYRDGIR DMHLSFIFR 
      QLAAPVIVS FGLALAVPY VLAYSIVPA FVSNLQMRN 
      LVARRIYPF LLLLGVVGA VVVLQIRQF KKLYEHIKN 
      DKYLVGQRL VNYDHRKAK SQTAT
    CDS sequence
    • >TC006591 RA
      atgatggac tcggaactg tccaacacc gacatctgc 
      cgggtgtgc cgttcggaa ggcttgccc gaccgcccc 
      ctgttccac ccctgcatc tgcacgggc agcataaag 
      tggatccat caggaatgc ctcatgcaa tggatgcgt 
      tactcgcgc aaagaatac tgcgaattg tgcagttat 
      cgtttctcc ttcactccc atctactcc cccgacatg 
      ccacgccgc ctccctgtg cgagacctc gccgccggt 
      ctcctctcc tccatcgga accgccatc aaatactgg 
      ctccactac actctagtc gccatcgct tggctcgga 
      atcgtccct ttgacagcc tgtcggatc taccgcgcc 
      cttttcgcc ggcactgtc gacgccatc ttgaccctt 
      ccgtttgaa ctcctctca atggaaaac ctggcatcg 
      gacattttc caaggttgc ttcgtcgtc acgtgcacg 
      ctattcgca ttcgctggg ttggtgtgg ctgcgcgag 
      cagatctta cacggggga ggacccgat tggttacag 
      agagataac gtgggcgtg gccgaagat gccccacct 
      cccgtcttg aataacaac ataccggaa gacgtggag 
      gagaacaac ggacaggag ccagaagta gcacctcaa 
      ggggagaat cagggggaa caacaggag gagaatgaa 
      gtgaatgga ggcgaagac aacaattgg atacccatg 
      gagtgggat agagcggcc gaggagata acatgggag 
      cgtttgttg ggtcttgat ggttcttta atgttcctg 
      gaacacgtt ttctgggtc atttcgttg aacactttg 
      tttatactc atttttgcc tactccccg taccatatg 
      ggtctaatg actctttcc cttgttaat atgagggag 
      gcggcttcg gctagtcat ttcgaaggt ttattgacc 
      actttggtc ggctattgc actgttggt cttttcttt 
      gtgttcttc catcgagtc gccggtgtt ttgggtttt 
      tacaaagct aagcgcgtc ctaggtttg tgttacgtg 
      gtggttaaa gtatcgatg ctttcggtg gttgaaatc 
      ggaattcta ccattggtt tgtggctgg tggttggat 
      atttgctct ttggcaatg tttgacgct tcgctcaaa 
      gacagggag agtagtttt agtgccgct cctggtact 
      tcaatcttc atccattgg ttgatcgga atggtctat 
      gtctattat ttcgcttcg tttgttctc ctcttgcgt 
      gaaattcta agaccagga gtgttgtgg ttcttgcgc 
      aacttcaac gaccctgat ttcagtcca attcaagaa 
      atgatccat ttacctatc ttggaacat atacggaaa 
      ttgttgatc tcggcggtt atcttcggt agtgttgtt 
      cttttgatg ttgtggctc ccgattcga attttgaaa 
      atccttctt ccgtctttt ctcccatat acagtgtcc 
      ctcaacagt gagacacaa gtgaatgaa ctgtcctta 
      gaattgtta cttttacaa gtgatcctc ccagcatta 
      ttggaacaa tcccacacg aggatctgg ttgaaaggc 
      ctggtgcgt aattggtgc cgcgccgtt gcctggatt 
      ttaaacatc cattcgtat ttgttgggc aatagtgac 
      ggccaatcg ccgaacgag gcggggcaa gctgtagcc 
      aatccgccc gaaccgggc gctggctta ggggcggcc 
      caccacgcc ctactaatg atggaaccg ccttctggt 
      ttccaaccc tacgagcgt ccgtccttt ttcgtcgcg 
      cgtctggta ggtctcctc atcttagtc tgtgtttcc 
      ctggtaacg ttcagcctc accgccttg accctgccc 
      gtgtggctg gggcggcgc gtcatggcg ttgtggctg 
      gtcggggcc ccgcccccc ggcccccaa atctcgaca 
      aactccgcc cagaccgag gtgaaagtc cacgaattg 
      tacacagcc gcgtgcggt ttgtacttg tgttggctg 
      tcggcgcgt gctgtgacc ttggtcatg agttggctg 
      ccccaaggc agggctgcc atgatttcg cgcttgaaa 
      cagtggtgc gtcttgggg cttaaaacc atgatcgct 
      agcacgatt ctactgggg gtgattccc ctactgttc 
      gggatgttg ctagagttg gtggttatc gtcccgctg 
      agggtgccg atccaccaa acgccgatt ttgttcatt 
      tggcaagac tgggcgctg ggcgtgttg tacacgaaa 
      atcgcgtgt gccgtcacg atgatgggc cctgattgg 
      ttccttcgg gcggccatc gaaagggcc tacagggat 
      gggataagg gatatgcat ctgagtttt atttttaga 
      cagttggcg gcgccggtt attgtcagt tttgggcta 
      gcgttggcc gtgccctat gtcttggcc tactcgatc 
      gtgccggcg tttgtgagt aatctacaa atgaggaat 
      ctagtagcc aggcggatc tatccgttt ttgctactg 
      ctcggtgtc gtgggtgcg gtggtagtt ttacaaatt 
      agacaattt aaaaaattg tacgaacat attaaaaac 
      gacaagtat ctagtaggc caaagattg gttaattac 
      gatcacagg aaagccaaa tcgcagacg gccacttag
    mRNA sequence
    • >TC006591 RA
      ggtattacg tcaaaaaat aaatttttt cgtcctgaa 
      aagcctcaa aacgcctaa aaaattgca gtttttgtc 
      gaatttcga gcatttgtg atcacgcaa ctatggatc 
      tttgagtcg gatccgtaa attacataa tgttcttca 
      cattcaagt agtttcctg tgccaaaaa acatcagcg 
      taatgatgg actcggaac tgtccaaca ccgacatct 
      gccgggtgt gccgttcgg aaggcttgc ccgaccgcc 
      ccctgttcc acccctgca tctgcacgg gcagcataa 
      agtggatcc atcaggaat gcctcatgc aatggatgc 
      gttactcgc gcaaagaat actgcgaat tgtgcagtt 
      atcgtttct ccttcactc ccatctact cccccgaca 
      tgccacgcc gcctccctg tgcgagacc tcgccgccg 
      gtctcctct cctccatcg gaaccgcca tcaaatact 
      ggctccact acactctag tcgccatcg cttggctcg 
      gaatcgtcc ctttgacag cctgtcgga tctaccgcg 
      cccttttcg ccggcactg tcgacgcca tcttgaccc 
      ttccgtttg aactcctct caatggaaa acctggcat 
      cggacattt tccaaggtt gcttcgtcg tcacgtgca 
      cgctattcg cattcgctg ggttggtgt ggctgcgcg 
      agcagatct tacacgggg gaggacccg attggttac 
      agagagata acgtgggcg tggccgaag atgccccac 
      ctcccgtct tgaataaca acataccgg aagacgtgg 
      aggagaaca acggacagg agccagaag tagcacctc 
      aaggggaga atcaggggg aacaacagg aggagaatg 
      aagtgaatg gaggcgaag acaacaatt ggataccca 
      tggagtggg atagagcgg ccgaggaga taacatggg 
      agcgtttgt tgggtcttg atggttctt taatgttcc 
      tggaacacg ttttctggg tcatttcgt tgaacactt 
      tgtttatac tcatttttg cctactccc cgtaccata 
      tgggtctaa tgactcttt cccttgtta atatgaggg 
      aggcggctt cggctagtc atttcgaag gtttattga 
      ccactttgg tcggctatt gcactgttg gtcttttct 
      ttgtgttct tccatcgag tcgccggtg ttttgggtt 
      tttacaaag ctaagcgcg tcctaggtt tgtgttacg 
      tggtggtta aagtatcga tgctttcgg tggttgaaa 
      tcggaattc taccattgg tttgtggct ggtggttgg 
      atatttgct ctttggcaa tgtttgacg cttcgctca 
      aagacaggg agagtagtt ttagtgccg ctcctggta 
      cttcaatct tcatccatt ggttgatcg gaatggtct 
      atgtctatt atttcgctt cgtttgttc tcctcttgc 
      gtgaaattc taagaccag gagtgttgt ggttcttgc 
      gcaacttca acgaccctg atttcagtc caattcaag 
      aaatgatcc atttaccta tcttggaac atatacgga 
      aattgttga tctcggcgg ttatcttcg gtagtgttg 
      ttcttttga tgttgtggc tcccgattc gaattttga 
      aaatccttc ttccgtctt ttctcccat atacagtgt 
      ccctcaaca gtgagacac aagtgaatg aactgtcct 
      tagaattgt tacttttac aagtgatcc tcccagcat 
      tattggaac aatcccaca cgaggatct ggttgaaag 
      gcctggtgc gtaattggt gccgcgccg ttgcctgga 
      ttttaaaca tccattcgt atttgttgg gcaatagtg 
      acggccaat cgccgaacg aggcggggc aagctgtag 
      ccaatccgc ccgaaccgg gcgctggct taggggcgg 
      cccaccacg ccctactaa tgatggaac cgccttctg 
      gtttccaac cctacgagc gtccgtcct ttttcgtcg 
      cgcgtctgg taggtctcc tcatcttag tctgtgttt 
      ccctggtaa cgttcagcc tcaccgcct tgaccctgc 
      ccgtgtggc tggggcggc gcgtcatgg cgttgtggc 
      tggtcgggg ccccgcccc ccggccccc aaatctcga 
      caaactccg cccagaccg aggtgaaag tccacgaat 
      tgtacacag ccgcgtgcg gtttgtact tgtgttggc 
      tgtcggcgc gtgctgtga ccttggtca tgagttggc 
      tgccccaag gcagggctg ccatgattt cgcgcttga 
      aacagtggt gcgtcttgg ggcttaaaa ccatgatcg 
      ctagcacga ttctactgg gggtgattc ccctactgt 
      tcgggatgt tgctagagt tggtggtta tcgtcccgc 
      tgagggtgc cgatccacc aaacgccga ttttgttca 
      tttggcaag actgggcgc tgggcgtgt tgtacacga 
      aaatcgcgt gtgccgtca cgatgatgg gccctgatt 
      ggttccttc gggcggcca tcgaaaggg cctacaggg 
      atgggataa gggatatgc atctgagtt ttattttta 
      gacagttgg cggcgccgg ttattgtca gttttgggc 
      tagcgttgg ccgtgccct atgtcttgg cctactcga 
      tcgtgccgg cgtttgtga gtaatctac aaatgagga 
      atctagtag ccaggcgga tctatccgt ttttgctac 
      tgctcggtg tcgtgggtg cggtggtag ttttacaaa 
      ttagacaat ttaaaaaat tgtacgaac atattaaaa 
      acgacaagt atctagtag gccaaagat tggttaatt 
      acgatcaca ggaaagcca aatcgcaga cggccactt 
      agaaatttt attgcgttt ttcacggtt aggttcatt 
      cctgtacat attattatt tattgtaaa tatggaaaa 
      tttaagttt ttggtttct ttctcctag atctacttt 
      cattcgaag gtgaggttg gtgcacttg agcttgtaa 
      ttgaagtta agtctgtga taataaaaa gcactttga 
      atcaataat tgaattgaa atctggtta ttcattcgt 
      acaacaaag tcggcacta atctcatca tgaattact 
      ttacgtaga ttgaaacgt taaagattt ttttaagtt 
      actatacct ttattttac ataataaag actgttttt 
      tgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY