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Gene information
General information
TC006575 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11516280..11518781
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006575
    iBeetle screen iB_04174

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006575 RA
      MTIIGICGG SERGDLELT DKANYVKNE EGKRSYTCG 
      QNLLNYVVE FCNNGTIHG IRYFGEYRT WIEKLWWAI 
      SICLSLYFC ITLIIQTYI KWETSPVIV SFATKETAV 
      WQIPFPAVT ICPESKTRA SVFNYTHYL HKKQDNEVL 
      SEEEERKFG YLSFICDTK FDIEYKSPK YADEEMLEF 
      LEQVQPKFL EDSFDCKFL GIPYNCSEL FVPILTDDG 
      ICYVFNIVD RTQLLSDIV VHFKNYHKT QVSHDWNLE 
      QGYAPTAGI DAYPRRALR AGIPYALEI KMAVHVDDL 
      DYTCGTDVK GFKVQIAHP QRMPRVKQQ HFIVPLERV 
      AKAGIAPDM MTTSVEVKE YKPRKRNCH FPSERGLKY 
      FKKYSQNNC AIECWANFT MSRCGCVPF YVPREESVP 
      LCGAGSLHC LEEAQKYQF RGNLEKKID ALSNKKTHS 
      DIPDCRCFP ICTSMYYDV ENTQATWDW RTTYKYNKH 
      KRIDVNDKK KRMSMLQVF FKGNQFIYS ERNELYGST 
      DLLANVGGL LGLFIGFSA LSLVEIIYY LTLRIICNV 
      AMFGRRNWS GTVN
    CDS sequence
    • >TC006575 RA
      atgaccatc ataggaatc tgtggtggc agtgaacgt 
      ggtgatctc gaactcacc gacaaagca aactacgtg 
      aaaaatgaa gaagggaaa cggagttac acttgcggc 
      caaaacctc ctcaattac gtggtggag ttttgcaac 
      aacggcaca atacacgga attcgctac ttcggagag 
      taccggact tggatcgag aaattgtgg tgggccatt 
      agtatctgt ctatcgctc tatttttgt ataacttta 
      atcatacaa acgtacatt aaatgggaa acttcccca 
      gttattgta agtttcgcg actaaagaa acagccgtc 
      tggcaaatc cctttcccg gccgttacc atttgtccc 
      gagtccaaa acccgggct tccgttttt aattacacg 
      cactacttg cataaaaaa caagacaac gaagttcta 
      agtgaagaa gaggaacgc aaattcgga tacttgtcg 
      tttatttgc gacacgaag ttcgacatt gagtacaaa 
      tcgccgaaa tacgccgat gaagaaatg ttagagttt 
      cttgaacaa gtccaaccc aaattcctg gaggactcg 
      ttcgactgc aaattcctg ggaattcca tacaattgc 
      tccgaatta ttcgtcccc attttgaca gatgatgga 
      atctgctac gtttttaac atcgtcgac agaacccaa 
      cttttgagt gacatagtg gttcatttt aagaactac 
      cacaaaacc caagtttcg cacgactgg aacctggag 
      cagggctac gcgcccacg gcggggatt gacgcctac 
      ccccgaagg gcccttcgg gcgggaatc ccctacgcc 
      cttgagatc aaaatggcg gttcacgtg gatgatttg 
      gactacacg tgtgggact gacgtgaaa gggtttaaa 
      gtccaaata gcgcacccg caaaggatg cctcgagtg 
      aaacaacag cactttata gtgcctttg gaacgcgtg 
      gccaaagct gggattgct ccagatatg atgacgacc 
      tctgtcgag gtcaaggag tacaaaccg aggaagcgc 
      aactgtcat ttcccctca gaacgaggg ttgaaatat 
      tttaaaaaa tacagtcag aataattgt gcgattgag 
      tgttgggcc aattttacg atgagtcgg tgcggatgt 
      gtaccattt tatgtacca cgggaagaa agtgtgccg 
      ttgtgtggg gctggaagc ctacactgc ctggaggag 
      gcccaaaaa taccagttt cggggcaat cttgagaaa 
      aaaatcgac gcattgagt aataaaaaa acccacagt 
      gatattccc gactgtcgt tgtttcccc atttgtacg 
      tcgatgtat tacgacgtt gaaaatacc caagccacg 
      tgggactgg aggacgact tacaaatac aacaagcat 
      aaacgtatc gatgttaat gacaaaaag aaacgcatg 
      tccatgtta caggttttt ttcaaaggg aaccaattc 
      atttattcg gagaggaat gagttgtac ggatcgacc 
      gatttgttg gccaatgtt ggaggtctg ctggggttg 
      tttatcggt ttttcggcg ctgagtctc gtggagatt 
      atttattat ttgactttg aggattatc tgcaatgtt 
      gcgatgttt ggacgcagg aactggtca ggaaccgta 
      aactga
    mRNA sequence
    • >TC006575 RA
      tgttgttgc tacaatgac catcatagg aatctgtgg 
      tggcagtga acgtggtga tctcgaact caccgacaa 
      agcaaacta cgtgaaaaa tgaagaagg gaaacggag 
      ttacacttg cggccaaaa cctcctcaa ttacgtggt 
      ggagttttg caacaacgg cacaataca cggaattcg 
      ctacttcgg agagtaccg gacttggat cgagaaatt 
      gtggtgggc cattagtat ctgtctatc gctctattt 
      ttgtataac tttaatcat acaaacgta cattaaatg 
      ggaaacttc cccagttat tgtaagttt cgcgactaa 
      agaaacagc cgtctggca aatcccttt cccggccgt 
      taccatttg tcccgagtc caaaacccg ggcttccgt 
      ttttaatta cacgcacta cttgcataa aaaacaaga 
      caacgaagt tctaagtga agaagagga acgcaaatt 
      cggatactt gtcgtttat ttgcgacac gaagttcga 
      cattgagta caaatcgcc gaaatacgc cgatgaaga 
      aatgttaga gtttcttga acaagtcca acccaaatt 
      cctggagga ctcgttcga ctgcaaatt cctgggaat 
      tccatacaa ttgctccga attattcgt ccccatttt 
      gacagatga tggaatctg ctacgtttt taacatcgt 
      cgacagaac ccaactttt gagtgacat agtggttca 
      ttttaagaa ctaccacaa aacccaagt ttcgcacga 
      ctggaacct ggagcaggg ctacgcgcc cacggcggg 
      gattgacgc ctacccccg aagggccct tcgggcggg 
      aatccccta cgcccttga gatcaaaat ggcggttca 
      cgtggatga tttggacta cacgtgtgg gactgacgt 
      gaaagggtt taaagtcca aatagcgca cccgcaaag 
      gatgcctcg agtgaaaca acagcactt tatagtgcc 
      tttggaacg cgtggccaa agctgggat tgctccaga 
      tatgatgac gacctctgt cgaggtcaa ggagtacaa 
      accgaggaa gcgcaactg tcatttccc ctcagaacg 
      agggttgaa atattttaa aaaatacag tcagaataa 
      ttgtgcgat tgagtgttg ggccaattt tacgatgag 
      tcggtgcgg atgtgtacc attttatgt accacggga 
      agaaagtgt gccgttgtg tggggctgg aagcctaca 
      ctgcctgga ggaggccca aaaatacca gtttcgggg 
      caatcttga gaaaaaaat cgacgcatt gagtaataa 
      aaaaaccca cagtgatat tcccgactg tcgttgttt 
      ccccatttg tacgtcgat gtattacga cgttgaaaa 
      tacccaagc cacgtggga ctggaggac gacttacaa 
      atacaacaa gcataaacg tatcgatgt taatgacaa 
      aaagaaacg catgtccat gttacaggt ttttttcaa 
      agggaacca attcattta ttcggagag gaatgagtt 
      gtacggatc gaccgattt gttggccaa tgttggagg 
      tctgctggg gttgtttat cggtttttc ggcgctgag 
      tctcgtgga gattattta ttatttgac tttgaggat 
      tatctgcaa tgttgcgat gtttggacg caggaactg 
      gtcaggaac cgtaaactg attgttttg tggtaatct 
      gtattatgt tgttaaaac ttgaaaata ataaaactc 
      gttaaaaac t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY