| General information | |||
| TC006551 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 11342749..11376594 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006551 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04170 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC006551 RA
MDKCGINAG GALSVLVKS GSGIVIPGA AITDHHGDR HRCPDSGAR ASRMPVEED YGRRQITLQ QHASQSPVP PSLQCPVGC IPAPADHRY ASDKNLLDP SERYSDRFG SERSNSDRL PSGERYQGV SERYPLGER PQTSVSSER LQPAEERTQ FQTERYHYA TCQRYPERY TPVGSLDRY HTHTVDRYS RTSTPTDRY HTLSDKDKT NASDRYTPI EKYQGENFS ANDRHNSAQ SVCSIERYT PTERHRSKS SDMYKIRER SSSSDRKTE RYQQYQNRY DQYVPERFP AIPCPERFA TQERNERVP FTQVPYMEP PSPAPASDR FVPPPPLSP ENTPSPDCF PNNAFPSPT NTVPPPPER FIPPPPLSP SPTEKYSPK KVERYEKQQ RYYDRYHRT QYYPQNERY HVNPDRFVT NDRYIPPNA HMPVERYVP QQQEPYYNT YQTYERYPK FNANDPYMR RDLAFHYRL PLPYSSTQY QRIRYVGTP NRVKCCQYQ EGYQLSKSS PGSSSSSSV TSQGKDLHQ KEVQCVNNS SLQEMQCQS YQTCAYQQE KGTQCGKEC VGFVSPNLR AKGQCRHSI CASPSVEYV GASGGRHVC ATPPPRGSI GSADGAVCN DNCCTARRA QNSLTVAVC SHKNLSRSI GRMELDDLR AAAPSQPPA GPAAAVAAA AARRHPELR QPQHSPKIP PVQRAVSLP ATSPPERPR MQPRLGYTQ SERLPNRPT LARSLSRKE MIKNYIKKE TATFFGVDE ESEEKQQRR WLDRRIRMA SRTYGPLKD EYRTLGSTY RRTISEMAP GGRMSGQLQ AERPDVLPG PNDVVDTNQ ERLHEVTVR RKDSVARMT WDGLSYVVN TLTRHRPRR RSQQSQQSR SFPPDTPYS SSSVEEETF FQKPTSPTS PRPQEEGDT IDRGHEATL QPAYRDRLT IRPGSGGWR RDPHVVLRH EGQNRPAVG GSRVFSSLL DNVLDNSNR RQYGMGWVG RMFGRSMRH SVTQDDKIL QQLDDMEDH RPMFTYWVT TVQILVLFI SLVCYGLGP FGIDLHSRS GQVLVTSLS LQQVDYMEP ANFWFGPRA SDLIHLGAK FAPCMRIDD KISQQIDKI RAKERETAC CIRNDDSGC VQSSQADCS KTISKWKKW SAGEAGPGG RISGSVCGL DPKFCDAPA SVHPYEWPD DITKWPICR KTNTQFSIQ KRSGSRDKS AEHMVCEVI GHPCCIGIH GQCKITTKE YCDFVQGTF HEEASLCSQ VSCLNDVCG MLPFYFAET PDQFYRLWT SLFLHAGVL QLAVTVLIQ YFLMRDLEK LTGSLRIGI IYIGSGVAG NLASAIFVP YRADVGPAG SQFGLLACL IVEVLNSWP MLKHPNQAL CKLLSITLV LFLIGLLPW VDNYAHLFG FVFGFLLSY ALLPFISFG VYERRKKIV LIWVCLVSA GVLFICLVL LFYIIPVYD CKICSYFNC IPFTRDFCA SQNINFKRE EPIV
- >TC006551 RB
MDKCGINAG GALSVLVKS GSGIVIPGA AITDHHGDR HRCPDSGAR ASRMPVEED YGRRQITLQ QHASQSPVP PSLQCPVGC IPAPADHRY ASDKNLLDP SERYSDRFG SERSNSDRL PSGERYQGV SERYPLGER PQTSVSSER LQPAEERTQ FQTERYHYA TCQRYPERY TPVGSLDRY HTHTVDRYS RTSTPTDRY HTLSDKDKT NASDRYTPI EKYQGENFS ANDRHNSAQ SVCSIERYT PTERHRSKS SDMYKIRER SSSSDRKTE RYQQYQNRY DQYVPERFP AIPCPERFA TQERNERVP FTQVPYMEP PSPAPASDR FVPPPPLSP ENTPSPDCF PNNAFPSPT NTVPPPPER FIPPPPLSP SPTEKYSPK KVERYEKQQ RYYDRYHRT QYYPQNERY HVNPDRFVT NDRYIPPNA HMPVERYVP QQQEPYYNT YQTYERYPK FNANDPYMR RDLAFHYRL PLPYSSTQY QRIRYVGTP NRVKCCQYQ EGYQLSKSS PGSSSSSSV TSQGKDLHQ KEVQCVNNS SLQEMQCQS YQTCAYQQE KGTQCGKEC VGFVSPNLR AKGQCRHSI CASPSVEYV GASGGRHVC ATPPPRGSI GSADGAVCN DNCCTARRA QNSLTVAVC SHKNLSRSI GRMELDDLR AAAPSQPPA GPAAAVAAA AARRHPELR QPQHSPKIP PVQRAVSLP ATSPPERPR MQPRLGYTQ SERLPNRPT LARSLSRKE MIKNYIKKE TATFFGVDE ESEEKQQRR WLDRRIRMA SRTYGPLKD EYRTLGSTY RRTISEMAP GGRMSGQLQ AERPDVLPG PNDVVDTNQ ERLHEVTVR RKDSVARMT WDGLSYVVN TLTRHRPRR RSQQSQQSR SFPPDTPYS SSSVEEETF FQKPTSPTS PRPQEEGDT IDRGHEATL QPAYRDRLT IRPGSGGWR RDPHVVLRH EGQNRPAVG GSRVFSSLL DNVLDNSNR RQYGMGWVG RMFGRSMRH SVTQDDKIL QQLDDMEDH RPMFTYWVT TVQILVLFI SLVCYGLGP FGIDLHSRS GQVLVTSLS LQQVDYMEP ANFWFGPRA SDLIHLGAK FAPCMRIDD KISQQIDKI RAKERETAC CIRNDDSGC VQSSQADCS LRGLRPTKT ISKWKKWSA GEAGPGGRI SGSVCGLDP KFCDAPASV HPYEWPDDI TKWPICRKT NTQFSIQKR SGSRDKSAE HMVCEVIGH PCCIGIHGQ CKITTKEYC DFVQGTFHE EASLCSQVS CLNDVCGML PFYFAETPD QFYRLWTSL FLHAGVLQL AVTVLIQYF LMRDLEKLT GSLRIGIIY IGSGVAGNL ASAIFVPYR ADVGPAGSQ FGLLACLIV EVLNSWPML KHPNQALCK LLSITLVLF LIGLLPWVD NYAHLFGFV FGFLLSYAL LPFISFGVY ERRKKIVLI WVCLVSAGV LFICLVLLF YIIPVYDCK ICSYFNCIP FTRDFCASQ NINFKREEP IV
- >TC006551 RA
atggataag tgtgggatt aacgctgga ggggccctt tcagtatta gtgaagagc ggaagcggc atcgtcata cctggcgcc gccatcacg gatcaccac ggcgaccgt cacagatgt cctgacagc ggggccagg gcctccaga atgcctgtg gaagaagat tatggcaga agacagatt acgctacag cagcatgct tcccagtcg ccggtaccg ccgtcccta cagtgtccc gttggttgt attcctgcg cctgcagat catcgctat gcaagcgat aagaatttg ctcgatccc tccgaacgc tactcagac aggttcggc tcggagcgc agcaattcg gatcggctg ccgtcggga gaacggtac cagggggtc tcggagcgg tacccgttg ggggagagg cctcagacc tccgtgtcc tccgaacgc ttgcagccc gcggaggaa cgcacgcag ttccagacg gaacggtac cactatgcc acgtgtcag aggtacccc gagaggtac acccccgtc ggcagtctc gataggtac cacacgcac actgtcgat aggtactcg aggacgagc accccgacc gatcggtac cacaccctg tccgacaaa gacaaaact aacgcttcc gatagatac acacccatc gagaagtac caaggggag aacttcagt gccaacgat aggcacaat tccgcacaa agcgtgtgt tccatcgaa cgttacacg ccgacggaa cgccacaga agcaaatcg agcgacatg tacaaaatc cgggagaga tcgtctagt tcggatagg aaaacggaa cggtaccag cagtaccag aaccggtac gaccaatac gttcctgaa cgctttccc gctattccg tgtcctgaa cggttcgcg acgcaggag cggaacgaa cgcgtacct ttcactcaa gtaccttac atggaacca ccttctcca gctccggca agcgataga ttcgtacca ccgccgcct ctatccccc gagaacaca ccaagtccc gactgtttt cctaataac gcttttcct tctcctact aacacggta ccaccccca cccgaacgt ttcatccca ccaccgcca ctatcacct tcgcccaca gaaaagtac tcccctaag aaagtcgaa aggtacgaa aaacagcaa cggtactac gaccggtac caccgcact caatactac ccccagaac gaacgctac catgtaaac cccgatcgc ttcgtcacc aacgaccgc tacatcccg cccaacgcc cacatgccc gtcgaacgc tacgtcccc caacaacag gaaccctac tacaacact taccaaacc tacgaacgg taccccaaa ttcaacgcg aacgacccc tacatgcga cgcgaccta gcgttccac taccgcctc ccgctccct tactcctcc acccagtac cagagaatc cgctatgtg ggtaccccg aaccgcgtc aagtgttgc cagtaccaa gaggggtac caactatcc aaatccagt cccggttcc agttcgagc agttcagtg acgagccaa ggcaaagac ctccaccag aaagaagtg caatgtgtc aacaactcc agtcttcaa gaaatgcag tgccaaagc taccaaacc tgtgcgtac caacaggaa aaggggacg cagtgcggc aaagagtgc gtcggtttt gtgtcgcct aatttacgc gcgaaagga caatgtcgc cacagcatc tgcgccagt ccgagcgtg gagtacgtg ggggcctcc gggggcagg cacgtgtgc gccacgccg cccccgagg ggcagcatc ggctcggcc gatggggcc gtctgcaac gacaactgc tgcacggca cggagagca cagaactcc ttgaccgtg gctgtttgt tcgcataaa aacttatct cggagtatc ggtagaatg gaattagac gatctgagg gcggcggcg ccttcgcag ccgccagcc ggcccagca gcagccgtc gccgccgca gcagcgcgc cgccacccc gagcttcgc cagccccaa cactcgccg aaaatccca cccgtccaa agggcggtt tccttgccg gcgacctct cccccagag cgcccccga atgcagccc cgtctgggg tacacccag agcgagagg ctgccaaat cggccgacg ttggctcgg tctttatcg cgcaaagaa atgatcaag aattacata aagaaggaa acggccact tttttcggt gtggatgaa gagagcgag gagaagcag cagaggagg tggctggat cggcggatc aggatggct tccaggacg tatgggccg ttaaaagat gagtacagg acgttgggg tcgacgtat agacgaacc atttccgag atggctccg ggcggccgg atgtcggga cagttacaa gctgaaaga cctgatgtt cttccgggg ccgaacgat gttgtggac accaatcag gagcggctg catgaggtc acggtccgt cggaaggac tcggtggcg aggatgacc tgggacggc ctgtcctac gtcgtcaat accttgacg agacaccgg cccagaagg cgctcacag cagagccaa caatcgagg agttttcca cctgacact ccttacagt tccagtagt gtggaagag gagacattt ttccaaaaa ccgacctct cccacctcg ccccggcca caggaggaa ggcgacacg atagacagg ggtcacgaa gccaccctt cagccagcg tacagggac cggttaacc atccgcccc ggttcaggg ggctggaga agggacccc catgtggtg ctccgacac gaggggcaa aatcggccc gctgttgga ggctcgagg gtgttttcg tcgctgctt gacaatgtt ttggacaat tccaatcgg cggcagtat ggcatgggt tgggtgggg cgaatgttc gggcgttcg atgcggcat tcggtgact caagacgac aagatttta caacaattg gacgacatg gaggaccat agaccgatg ttcacttat tgggtgacc acagtacaa attttagtt ttgttcatt tctttggtg tgttacggg ttggggcct ttcggcatt gacttgcat tcaaggtcg ggacaggtc ctagtgact agtttgtcg ctacagcag gtggattac atggaaccg gcgaatttt tggtttggt ccaagagcc agcgatttg attcatttg ggggcgaaa ttcgcccct tgtatgcgg atagatgat aagatttcg caacagatt gataagatc agggcaaaa gaacgggaa acagcgtgt tgtatcagg aatgacgat tcaggttgt gtgcagtca tcacaggcc gattgttct aaaactata tcgaagtgg aagaagtgg agcgcgggt gaggcgggc cccgggggc cgcatttcg ggctccgtc tgcggtttg gacccaaaa ttttgcgac gcccccgcg agtgtgcac ccgtacgaa tggccggac gacatcaca aaatggccc atttgtcgc aagaccaac acccaattc tccattcag aaacgtagc ggaagtcgc gataaaagc gccgaacac atggtctgc gaagtgatc ggacacccg tgctgtatc ggaatccac ggccagtgc aaaatcacc acgaaggag tactgcgat ttcgtccag gggacgttt cacgaggaa gcttcgctg tgctcgcag gtatcttgt ctaaacgac gtgtgtgga atgctcccg ttctacttc gccgaaacc cccgatcag ttctaccgc ctgtggacc tccctgttc ctccacgct ggagtcctc caactagcc gtcactgtc ctcatccag tacttctta atgcgcgat ttggaaaaa ctgactggg agtctccgc atcggcatc atctacata ggctcgggg gttgccggc aacctcgcc agcgccatc ttcgtgccc tatcgagcc gatgttggt cccgcgggc tcccagttc ggcctgttg gcgtgcctg atcgtggaa gtgctcaac tcgtggccc atgctgaag cacccgaac caggcccta tgtaagctc ctttccatc actctcgtc ctcttcctc atcggcctc ctgccttgg gtcgataat tacgctcat ttgttcggt ttcgtgttc ggttttctg ttatcgtac gcgttgttg ccgtttatt tcgttcggt gtttacgaa agacgcaag aagatcgtc ctgatctgg gtgtgtctg gtgtcggct ggggtgctc ttcatctgt ctggtcctc ctcttctac atcataccg gtctacgac tgcaaaatc tgctcgtac ttcaactgt ataccgttc acgcgcgat ttctgtgct tcgcaaaat attaatttc aaaagggag gaacctata gtatga
- >TC006551 RB
atggataag tgtgggatt aacgctgga ggggccctt tcagtatta gtgaagagc ggaagcggc atcgtcata cctggcgcc gccatcacg gatcaccac ggcgaccgt cacagatgt cctgacagc ggggccagg gcctccaga atgcctgtg gaagaagat tatggcaga agacagatt acgctacag cagcatgct tcccagtcg ccggtaccg ccgtcccta cagtgtccc gttggttgt attcctgcg cctgcagat catcgctat gcaagcgat aagaatttg ctcgatccc tccgaacgc tactcagac aggttcggc tcggagcgc agcaattcg gatcggctg ccgtcggga gaacggtac cagggggtc tcggagcgg tacccgttg ggggagagg cctcagacc tccgtgtcc tccgaacgc ttgcagccc gcggaggaa cgcacgcag ttccagacg gaacggtac cactatgcc acgtgtcag aggtacccc gagaggtac acccccgtc ggcagtctc gataggtac cacacgcac actgtcgat aggtactcg aggacgagc accccgacc gatcggtac cacaccctg tccgacaaa gacaaaact aacgcttcc gatagatac acacccatc gagaagtac caaggggag aacttcagt gccaacgat aggcacaat tccgcacaa agcgtgtgt tccatcgaa cgttacacg ccgacggaa cgccacaga agcaaatcg agcgacatg tacaaaatc cgggagaga tcgtctagt tcggatagg aaaacggaa cggtaccag cagtaccag aaccggtac gaccaatac gttcctgaa cgctttccc gctattccg tgtcctgaa cggttcgcg acgcaggag cggaacgaa cgcgtacct ttcactcaa gtaccttac atggaacca ccttctcca gctccggca agcgataga ttcgtacca ccgccgcct ctatccccc gagaacaca ccaagtccc gactgtttt cctaataac gcttttcct tctcctact aacacggta ccaccccca cccgaacgt ttcatccca ccaccgcca ctatcacct tcgcccaca gaaaagtac tcccctaag aaagtcgaa aggtacgaa aaacagcaa cggtactac gaccggtac caccgcact caatactac ccccagaac gaacgctac catgtaaac cccgatcgc ttcgtcacc aacgaccgc tacatcccg cccaacgcc cacatgccc gtcgaacgc tacgtcccc caacaacag gaaccctac tacaacact taccaaacc tacgaacgg taccccaaa ttcaacgcg aacgacccc tacatgcga cgcgaccta gcgttccac taccgcctc ccgctccct tactcctcc acccagtac cagagaatc cgctatgtg ggtaccccg aaccgcgtc aagtgttgc cagtaccaa gaggggtac caactatcc aaatccagt cccggttcc agttcgagc agttcagtg acgagccaa ggcaaagac ctccaccag aaagaagtg caatgtgtc aacaactcc agtcttcaa gaaatgcag tgccaaagc taccaaacc tgtgcgtac caacaggaa aaggggacg cagtgcggc aaagagtgc gtcggtttt gtgtcgcct aatttacgc gcgaaagga caatgtcgc cacagcatc tgcgccagt ccgagcgtg gagtacgtg ggggcctcc gggggcagg cacgtgtgc gccacgccg cccccgagg ggcagcatc ggctcggcc gatggggcc gtctgcaac gacaactgc tgcacggca cggagagca cagaactcc ttgaccgtg gctgtttgt tcgcataaa aacttatct cggagtatc ggtagaatg gaattagac gatctgagg gcggcggcg ccttcgcag ccgccagcc ggcccagca gcagccgtc gccgccgca gcagcgcgc cgccacccc gagcttcgc cagccccaa cactcgccg aaaatccca cccgtccaa agggcggtt tccttgccg gcgacctct cccccagag cgcccccga atgcagccc cgtctgggg tacacccag agcgagagg ctgccaaat cggccgacg ttggctcgg tctttatcg cgcaaagaa atgatcaag aattacata aagaaggaa acggccact tttttcggt gtggatgaa gagagcgag gagaagcag cagaggagg tggctggat cggcggatc aggatggct tccaggacg tatgggccg ttaaaagat gagtacagg acgttgggg tcgacgtat agacgaacc atttccgag atggctccg ggcggccgg atgtcggga cagttacaa gctgaaaga cctgatgtt cttccgggg ccgaacgat gttgtggac accaatcag gagcggctg catgaggtc acggtccgt cggaaggac tcggtggcg aggatgacc tgggacggc ctgtcctac gtcgtcaat accttgacg agacaccgg cccagaagg cgctcacag cagagccaa caatcgagg agttttcca cctgacact ccttacagt tccagtagt gtggaagag gagacattt ttccaaaaa ccgacctct cccacctcg ccccggcca caggaggaa ggcgacacg atagacagg ggtcacgaa gccaccctt cagccagcg tacagggac cggttaacc atccgcccc ggttcaggg ggctggaga agggacccc catgtggtg ctccgacac gaggggcaa aatcggccc gctgttgga ggctcgagg gtgttttcg tcgctgctt gacaatgtt ttggacaat tccaatcgg cggcagtat ggcatgggt tgggtgggg cgaatgttc gggcgttcg atgcggcat tcggtgact caagacgac aagatttta caacaattg gacgacatg gaggaccat agaccgatg ttcacttat tgggtgacc acagtacaa attttagtt ttgttcatt tctttggtg tgttacggg ttggggcct ttcggcatt gacttgcat tcaaggtcg ggacaggtc ctagtgact agtttgtcg ctacagcag gtggattac atggaaccg gcgaatttt tggtttggt ccaagagcc agcgatttg attcatttg ggggcgaaa ttcgcccct tgtatgcgg atagatgat aagatttcg caacagatt gataagatc agggcaaaa gaacgggaa acagcgtgt tgtatcagg aatgacgat tcaggttgt gtgcagtca tcacaggcc gattgttct ctgagagga ttaagacct acgaaaact atatcgaag tggaagaag tggagcgcg ggtgaggcg ggccccggg ggccgcatt tcgggctcc gtctgcggt ttggaccca aaattttgc gacgccccc gcgagtgtg cacccgtac gaatggccg gacgacatc acaaaatgg cccatttgt cgcaagacc aacacccaa ttctccatt cagaaacgt agcggaagt cgcgataaa agcgccgaa cacatggtc tgcgaagtg atcggacac ccgtgctgt atcggaatc cacggccag tgcaaaatc accacgaag gagtactgc gatttcgtc caggggacg tttcacgag gaagcttcg ctgtgctcg caggtatct tgtctaaac gacgtgtgt ggaatgctc ccgttctac ttcgccgaa acccccgat cagttctac cgcctgtgg acctccctg ttcctccac gctggagtc ctccaacta gccgtcact gtcctcatc cagtacttc ttaatgcgc gatttggaa aaactgact gggagtctc cgcatcggc atcatctac ataggctcg ggggttgcc ggcaacctc gccagcgcc atcttcgtg ccctatcga gccgatgtt ggtcccgcg ggctcccag ttcggcctg ttggcgtgc ctgatcgtg gaagtgctc aactcgtgg cccatgctg aagcacccg aaccaggcc ctatgtaag ctcctttcc atcactctc gtcctcttc ctcatcggc ctcctgcct tgggtcgat aattacgct catttgttc ggtttcgtg ttcggtttt ctgttatcg tacgcgttg ttgccgttt atttcgttc ggtgtttac gaaagacgc aagaagatc gtcctgatc tgggtgtgt ctggtgtcg gctggggtg ctcttcatc tgtctggtc ctcctcttc tacatcata ccggtctac gactgcaaa atctgctcg tacttcaac tgtataccg ttcacgcgc gatttctgt gcttcgcaa aatattaat ttcaaaagg gaggaacct atagtatga
- >TC006551 RA
gcgtataca ggccgtgtt gacacggcg ccccatata acgccaggg ggcccaggg gcgagagac tgggtcggg atttcctcg cagtgagat acagtgggt ttttacatg agtgacgtt tcatggata agtgtggga ttaacgctg gaggggccc tttcagtat tagtgaaga gcggaagcg gcatcgtca tacctggcg ccgccatca cggatcacc acggcgacc gtcacagat gtcctgaca gcggggcca gggcctcca gaatgcctg tggaagaag attatggca gaagacaga ttacgctac agcagcatg cttcccagt cgccggtac cgccgtccc tacagtgtc ccgttggtt gtattcctg cgcctgcag atcatcgct atgcaagcg ataagaatt tgctcgatc cctccgaac gctactcag acaggttcg gctcggagc gcagcaatt cggatcggc tgccgtcgg gagaacggt accaggggg tctcggagc ggtacccgt tgggggaga ggcctcaga cctccgtgt cctccgaac gcttgcagc ccgcggagg aacgcacgc agttccaga cggaacggt accactatg ccacgtgtc agaggtacc ccgagaggt acacccccg tcggcagtc tcgataggt accacacgc acactgtcg ataggtact cgaggacga gcaccccga ccgatcggt accacaccc tgtccgaca aagacaaaa ctaacgctt ccgatagat acacaccca tcgagaagt accaagggg agaacttca gtgccaacg ataggcaca attccgcac aaagcgtgt gttccatcg aacgttaca cgccgacgg aacgccaca gaagcaaat cgagcgaca tgtacaaaa tccgggaga gatcgtcta gttcggata ggaaaacgg aacggtacc agcagtacc agaaccggt acgaccaat acgttcctg aacgctttc ccgctattc cgtgtcctg aacggttcg cgacgcagg agcggaacg aacgcgtac ctttcactc aagtacctt acatggaac caccttctc cagctccgg caagcgata gattcgtac caccgccgc ctctatccc ccgagaaca caccaagtc ccgactgtt ttcctaata acgcttttc cttctccta ctaacacgg taccacccc cacccgaac gtttcatcc caccaccgc cactatcac cttcgccca cagaaaagt actccccta agaaagtcg aaaggtacg aaaaacagc aacggtact acgaccggt accaccgca ctcaatact acccccaga acgaacgct accatgtaa accccgatc gcttcgtca ccaacgacc gctacatcc cgcccaacg cccacatgc ccgtcgaac gctacgtcc cccaacaac aggaaccct actacaaca cttaccaaa cctacgaac ggtacccca aattcaacg cgaacgacc cctacatgc gacgcgacc tagcgttcc actaccgcc tcccgctcc cttactcct ccacccagt accagagaa tccgctatg tgggtaccc cgaaccgcg tcaagtgtt gccagtacc aagaggggt accaactat ccaaatcca gtcccggtt ccagttcga gcagttcag tgacgagcc aaggcaaag acctccacc agaaagaag tgcaatgtg tcaacaact ccagtcttc aagaaatgc agtgccaaa gctaccaaa cctgtgcgt accaacagg aaaagggga cgcagtgcg gcaaagagt gcgtcggtt ttgtgtcgc ctaatttac gcgcgaaag gacaatgtc gccacagca tctgcgcca gtccgagcg tggagtacg tgggggcct ccgggggca ggcacgtgt gcgccacgc cgcccccga ggggcagca tcggctcgg ccgatgggg ccgtctgca acgacaact gctgcacgg cacggagag cacagaact ccttgaccg tggctgttt gttcgcata aaaacttat ctcggagta tcggtagaa tggaattag acgatctga gggcggcgg cgccttcgc agccgccag ccggcccag cagcagccg tcgccgccg cagcagcgc gccgccacc ccgagcttc gccagcccc aacactcgc cgaaaatcc cacccgtcc aaagggcgg tttccttgc cggcgacct ctcccccag agcgccccc gaatgcagc cccgtctgg ggtacaccc agagcgaga ggctgccaa atcggccga cgttggctc ggtctttat cgcgcaaag aaatgatca agaattaca taaagaagg aaacggcca cttttttcg gtgtggatg aagagagcg aggagaagc agcagagga ggtggctgg atcggcgga tcaggatgg cttccagga cgtatgggc cgttaaaag atgagtaca ggacgttgg ggtcgacgt atagacgaa ccatttccg agatggctc cgggcggcc ggatgtcgg gacagttac aagctgaaa gacctgatg ttcttccgg ggccgaacg atgttgtgg acaccaatc aggagcggc tgcatgagg tcacggtcc gtcggaagg actcggtgg cgaggatga cctgggacg gcctgtcct acgtcgtca ataccttga cgagacacc ggcccagaa ggcgctcac agcagagcc aacaatcga ggagttttc cacctgaca ctccttaca gttccagta gtgtggaag aggagacat ttttccaaa aaccgacct ctcccacct cgccccggc cacaggagg aaggcgaca cgatagaca ggggtcacg aagccaccc ttcagccag cgtacaggg accggttaa ccatccgcc ccggttcag ggggctgga gaagggacc cccatgtgg tgctccgac acgaggggc aaaatcggc ccgctgttg gaggctcga gggtgtttt cgtcgctgc ttgacaatg ttttggaca attccaatc ggcggcagt atggcatgg gttgggtgg ggcgaatgt tcgggcgtt cgatgcggc attcggtga ctcaagacg acaagattt tacaacaat tggacgaca tggaggacc atagaccga tgttcactt attgggtga ccacagtac aaattttag ttttgttca tttctttgg tgtgttacg ggttggggc ctttcggca ttgacttgc attcaaggt cgggacagg tcctagtga ctagtttgt cgctacagc aggtggatt acatggaac cggcgaatt tttggtttg gtccaagag ccagcgatt tgattcatt tgggggcga aattcgccc cttgtatgc ggatagatg ataagattt cgcaacaga ttgataaga tcagggcaa aagaacggg aaacagcgt gttgtatca ggaatgacg attcaggtt gtgtgcagt catcacagg ccgattgtt ctaaaacta tatcgaagt ggaagaagt ggagcgcgg gtgaggcgg gccccgggg gccgcattt cgggctccg tctgcggtt tggacccaa aattttgcg acgcccccg cgagtgtgc acccgtacg aatggccgg acgacatca caaaatggc ccatttgtc gcaagacca acacccaat tctccattc agaaacgta gcggaagtc gcgataaaa gcgccgaac acatggtct gcgaagtga tcggacacc cgtgctgta tcggaatcc acggccagt gcaaaatca ccacgaagg agtactgcg atttcgtcc aggggacgt ttcacgagg aagcttcgc tgtgctcgc aggtatctt gtctaaacg acgtgtgtg gaatgctcc cgttctact tcgccgaaa cccccgatc agttctacc gcctgtgga cctccctgt tcctccacg ctggagtcc tccaactag ccgtcactg tcctcatcc agtacttct taatgcgcg atttggaaa aactgactg ggagtctcc gcatcggca tcatctaca taggctcgg gggttgccg gcaacctcg ccagcgcca tcttcgtgc cctatcgag ccgatgttg gtcccgcgg gctcccagt tcggcctgt tggcgtgcc tgatcgtgg aagtgctca actcgtggc ccatgctga agcacccga accaggccc tatgtaagc tcctttcca tcactctcg tcctcttcc tcatcggcc tcctgcctt gggtcgata attacgctc atttgttcg gtttcgtgt tcggttttc tgttatcgt acgcgttgt tgccgttta tttcgttcg gtgtttacg aaagacgca agaagatcg tcctgatct gggtgtgtc tggtgtcgg ctggggtgc tcttcatct gtctggtcc tcctcttct acatcatac cggtctacg actgcaaaa tctgctcgt acttcaact gtataccgt tcacgcgcg atttctgtg cttcgcaaa atattaatt tcaaaaggg aggaaccta tagtatgac aaataaggt tacggtttg taaccgtat tgttttatt tctttattg tttcggttg agagtagtt aagttttta caaatgttt gagttggtc gagttgttt tgtttggga ccctatcca acccggtgg caaaaagcg ttttaaaat caagtgaaa ggagttttc cccgaatgt tagttttgt gataaacag atccatgta aaatattgg gaaaaacag tgttgatat tgcgaactg atctttaaa acatgtgat acaaactat atctcggta tatttaaga cgttaattt atataagat atgtgcaat gaaatttga tttatgttg gcaacgaaa atacagcta cgaacttgg ttcagcccc tcctagtgg acgtttgct tcaataaag ttacgatca aaaagtgcc ctttaactg actctttta gccattctt ttgatttgg aagcgccac agttcggtt accctgcga cttattgaa gcaaattcg cggcaactc gatggatat gtaaccatt aacaataga ctcatttat atgtgttta cttataaat ttatcagaa gatattttc atcactatt atagaagaa aactgtata aattatata agcatgaat gattgtgta catataacg cagtatggt ggtgttaat gttaatttt tgtaaaatt accccgctc tgagaggaa tctttattt gtctacata acagtttca ctttgttgt aattttagg gccggcgct tccgccaac gttctagtc tcgaaaatg gccaaatcg tttccgttt gtttctttc gttgggaac gttgacgta acccgagct aatcgactt aacccacga tatatatta taatgtaat gatcgtaac gtctgtaga agttgcgtt tatcgtttt ggcccctat ttcgcattt ttgacaaat ccacttgtg atttcctgt gaaattctc tccgtatca tagccagtg atgtaaatc gattttata acagtagct atttatatt tgtgtaggc ttatgagtt ttcgacatt tttgactga tactcaaat atcaacgta cactgcaat tgtacaaaa gttgccatt ttcgtttac tacgctttc actcaaacg gctatctga tcggtttcc aagcagacg ttgcattgt gtattacga ctgacgatt acattacgt ttcaacaat tgtacagtt acattgtaa tattataaa atcaggtgt tatcactaa gaccaagcc ttaacatta taataacat aataaatta cttctgcac at
- >TC006551 RB
gccgtgttg acacggcgc cccatataa cgccagggg gcccagggg cgagagact gggtcggga tttcctcgc agtgagata cagtgggtt tttacatga gtgacgttt catggataa gtgtgggat taacgctgg aggggccct ttcagtatt agtgaagag cggaagcgg catcgtcat acctggcgc cgccatcac ggatcacca cggcgaccg tcacagatg tcctgacag cggggccag ggcctccag aatgcctgt ggaagaaga ttatggcag aagacagat tacgctaca gcagcatgc ttcccagtc gccggtacc gccgtccct acagtgtcc cgttggttg tattcctgc gcctgcaga tcatcgcta tgcaagcga taagaattt gctcgatcc ctccgaacg ctactcaga caggttcgg ctcggagcg cagcaattc ggatcggct gccgtcggg agaacggta ccagggggt ctcggagcg gtacccgtt gggggagag gcctcagac ctccgtgtc ctccgaacg cttgcagcc cgcggagga acgcacgca gttccagac ggaacggta ccactatgc cacgtgtca gaggtaccc cgagaggta cacccccgt cggcagtct cgataggta ccacacgca cactgtcga taggtactc gaggacgag caccccgac cgatcggta ccacaccct gtccgacaa agacaaaac taacgcttc cgatagata cacacccat cgagaagta ccaagggga gaacttcag tgccaacga taggcacaa ttccgcaca aagcgtgtg ttccatcga acgttacac gccgacgga acgccacag aagcaaatc gagcgacat gtacaaaat ccgggagag atcgtctag ttcggatag gaaaacgga acggtacca gcagtacca gaaccggta cgaccaata cgttcctga acgctttcc cgctattcc gtgtcctga acggttcgc gacgcagga gcggaacga acgcgtacc tttcactca agtacctta catggaacc accttctcc agctccggc aagcgatag attcgtacc accgccgcc tctatcccc cgagaacac accaagtcc cgactgttt tcctaataa cgcttttcc ttctcctac taacacggt accaccccc acccgaacg tttcatccc accaccgcc actatcacc ttcgcccac agaaaagta ctcccctaa gaaagtcga aaggtacga aaaacagca acggtacta cgaccggta ccaccgcac tcaatacta cccccagaa cgaacgcta ccatgtaaa ccccgatcg cttcgtcac caacgaccg ctacatccc gcccaacgc ccacatgcc cgtcgaacg ctacgtccc ccaacaaca ggaacccta ctacaacac ttaccaaac ctacgaacg gtaccccaa attcaacgc gaacgaccc ctacatgcg acgcgacct agcgttcca ctaccgcct cccgctccc ttactcctc cacccagta ccagagaat ccgctatgt gggtacccc gaaccgcgt caagtgttg ccagtacca agaggggta ccaactatc caaatccag tcccggttc cagttcgag cagttcagt gacgagcca aggcaaaga cctccacca gaaagaagt gcaatgtgt caacaactc cagtcttca agaaatgca gtgccaaag ctaccaaac ctgtgcgta ccaacagga aaaggggac gcagtgcgg caaagagtg cgtcggttt tgtgtcgcc taatttacg cgcgaaagg acaatgtcg ccacagcat ctgcgccag tccgagcgt ggagtacgt gggggcctc cgggggcag gcacgtgtg cgccacgcc gcccccgag gggcagcat cggctcggc cgatggggc cgtctgcaa cgacaactg ctgcacggc acggagagc acagaactc cttgaccgt ggctgtttg ttcgcataa aaacttatc tcggagtat cggtagaat ggaattaga cgatctgag ggcggcggc gccttcgca gccgccagc cggcccagc agcagccgt cgccgccgc agcagcgcg ccgccaccc cgagcttcg ccagcccca acactcgcc gaaaatccc acccgtcca aagggcggt ttccttgcc ggcgacctc tcccccaga gcgcccccg aatgcagcc ccgtctggg gtacaccca gagcgagag gctgccaaa tcggccgac gttggctcg gtctttatc gcgcaaaga aatgatcaa gaattacat aaagaagga aacggccac ttttttcgg tgtggatga agagagcga ggagaagca gcagaggag gtggctgga tcggcggat caggatggc ttccaggac gtatgggcc gttaaaaga tgagtacag gacgttggg gtcgacgta tagacgaac catttccga gatggctcc gggcggccg gatgtcggg acagttaca agctgaaag acctgatgt tcttccggg gccgaacga tgttgtgga caccaatca ggagcggct gcatgaggt cacggtccg tcggaagga ctcggtggc gaggatgac ctgggacgg cctgtccta cgtcgtcaa taccttgac gagacaccg gcccagaag gcgctcaca gcagagcca acaatcgag gagttttcc acctgacac tccttacag ttccagtag tgtggaaga ggagacatt tttccaaaa accgacctc tcccacctc gccccggcc acaggagga aggcgacac gatagacag gggtcacga agccaccct tcagccagc gtacaggga ccggttaac catccgccc cggttcagg gggctggag aagggaccc ccatgtggt gctccgaca cgaggggca aaatcggcc cgctgttgg aggctcgag ggtgttttc gtcgctgct tgacaatgt tttggacaa ttccaatcg gcggcagta tggcatggg ttgggtggg gcgaatgtt cgggcgttc gatgcggca ttcggtgac tcaagacga caagatttt acaacaatt ggacgacat ggaggacca tagaccgat gttcactta ttgggtgac cacagtaca aattttagt tttgttcat ttctttggt gtgttacgg gttggggcc tttcggcat tgacttgca ttcaaggtc gggacaggt cctagtgac tagtttgtc gctacagca ggtggatta catggaacc ggcgaattt ttggtttgg tccaagagc cagcgattt gattcattt gggggcgaa attcgcccc ttgtatgcg gatagatga taagatttc gcaacagat tgataagat cagggcaaa agaacggga aacagcgtg ttgtatcag gaatgacga ttcaggttg tgtgcagtc atcacaggc cgattgttc tctgagagg attaagacc tacgaaaac tatatcgaa gtggaagaa gtggagcgc gggtgaggc gggccccgg gggccgcat ttcgggctc cgtctgcgg tttggaccc aaaattttg cgacgcccc cgcgagtgt gcacccgta cgaatggcc ggacgacat cacaaaatg gcccatttg tcgcaagac caacaccca attctccat tcagaaacg tagcggaag tcgcgataa aagcgccga acacatggt ctgcgaagt gatcggaca cccgtgctg tatcggaat ccacggcca gtgcaaaat caccacgaa ggagtactg cgatttcgt ccaggggac gtttcacga ggaagcttc gctgtgctc gcaggtatc ttgtctaaa cgacgtgtg tggaatgct cccgttcta cttcgccga aacccccga tcagttcta ccgcctgtg gacctccct gttcctcca cgctggagt cctccaact agccgtcac tgtcctcat ccagtactt cttaatgcg cgatttgga aaaactgac tgggagtct ccgcatcgg catcatcta cataggctc gggggttgc cggcaacct cgccagcgc catcttcgt gccctatcg agccgatgt tggtcccgc gggctccca gttcggcct gttggcgtg cctgatcgt ggaagtgct caactcgtg gcccatgct gaagcaccc gaaccaggc cctatgtaa gctcctttc catcactct cgtcctctt cctcatcgg cctcctgcc ttgggtcga taattacgc tcatttgtt cggtttcgt gttcggttt tctgttatc gtacgcgtt gttgccgtt tatttcgtt cggtgttta cgaaagacg caagaagat cgtcctgat ctgggtgtg tctggtgtc ggctggggt gctcttcat ctgtctggt cctcctctt ctacatcat accggtcta cgactgcaa aatctgctc gtacttcaa ctgtatacc gttcacgcg cgatttctg tgcttcgca aaatattaa tttcaaaag ggaggaacc tatagtatg acaaataag gttacggtt tgtaaccgt attgtttta tttctttat tgtttcggt tgagagtag ttaagtttt tacaaatgt ttgagttgg tcgagttgt tttgtttgg gaccctatc caacccggt ggcaaaaag cgttttaaa atcaagtga aaggagttt tccccgaat gttagtttt gtgataaac agatccatg taaaatatt gggaaaaac agtgttgat attgcgaac tgatcttta aaacatgtg atacaaact atatctcgg tatatttaa gacgttaat ttatataag atatgtgca atgaaattt gatttatgt tggcaacga aaatacagc tacgaactt ggttcagcc cctcctagt ggacgtttg cttcaataa agttacgat caaaaagtg ccctttaac tgactcttt tagccattc ttttgattt ggaagcgcc acagttcgg ttaccctgc gacttattg aagcaaatt cgcggcaac tcgatggat atgtaacca ttaacaata gactcattt atatgtgtt tacttataa atttatcag aagatattt tcatcacta ttatagaag aaaactgta taaattata taagcatga atgattgtg tacatataa cgcagtatg gtggtgtta atgttaatt tttgtaaaa ttaccccgc tctgagagg aatctttat ttgtctaca taacagttt cactttgtt gtaatttta gggccggcg cttccgcca acgttctag tctcgaaaa tggccaaat cgtttccgt ttgtttctt tcgttggga acgttgacg taacccgag ctaatcgac ttaacccac gatatatat tataatgta atgatcgta acgtctgta gaagttgcg tttatcgtt ttggcccct atttcgcat ttttgacaa atccacttg tgatttcct gtgaaattc tctccgtat catagccag tgatgtaaa tcgatttta taacagtag ctatttata tttgtgtag gcttatgag ttttcgaca tttttgact gatactcaa atatcaacg tacactgca attgtacaa aagttgcca ttttcgttt actacgctt tcactcaaa cggctatct gatcggttt ccaagcaga cgttgcatt gtgtattac gactgacga ttacattac gtttcaaca attgtacag ttacattgt aatattata aaatcaggt gttatcact aagaccaag ccttaacat tataataac ataataaat tacttctgc acat
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